18 results about "Echinacoside" patented technology

The invention discloses a cistanche deserticola extract for improving bone mineral density, the total content of echinacoside and verbascoside in the cistanche deserticola extract is greater than 25wt%, and the cistanche deserticola extract is obtained by adding distilled water into cistanche tubulosa coarse powder for extraction, filtration, filtrate centrifugation, concentration, extraction, concentration and impurity removal, dilution, after-treatment, concentration and drying. The total content of echinacoside and verbascoside in the cistanche deserticola extract for improving the bone mineral density is 25-30 wt%, and the cistanche deserticola extract is rich in shikimic acid, phenylpropionic acid and alkaloid components, so that the cistanche deserticola extract can stably play a role in improving the bone mineral density.

The invention relates to the technical field of medicinal chemistry, and particularly discloses application of echinacoside in preparation of a medicine for improving autism social and cognitive impairment, and the application comprises the following steps: constructing a recombinant saccharomyces cerevisiae strain, and knocking out a precursor competition pathway gene; performing metabolic flow reprogramming fermentation on the recombinant strain; centrifuging the fermentation liquor at 7000-9000 r / min and pretreating the fermentation liquor with an 8-12 kDa ultrafiltration membrane; carrying out dual-targeting affinity chromatography purification on the pretreatment liquid; the preparation method comprises the following steps: preparing echinacoside into a liposome containing pH sensitive poly-histidine; and adding a freeze-drying protective agent into the suspension to carry out gradient pre-freezing and vacuum freeze-drying. According to the application, a mode of combining recombinant saccharomyces cerevisiae strain construction and metabolic flow reprogramming fermentation is adopted, targeted enrichment of echinacoside is realized through affinity chromatography coupled with autism brain region specific double ligands, then the echinacoside is prepared into the liposome containing pH sensitive poly-histidine, the double ligands are modified, and the effects of precise targeting and efficient effect are achieved.

The application belongs to the technical field of medicine, and particularly relates to application of echinacoside in preparation of a medicine for preventing or treating a demyelination disease; the echinacoside can promote differentiation and maturation of OPCs to OLs, promote proliferation of OLs, promote myelin repair, improve pathological changes of myelin ultrastructure, and promote myelin thickness to return to a normal level; the scheme provides a new treatment means for preventing or treating the demyelination disease, and is expected to reverse disability caused by myelin shedding or damage.

The invention discloses health-preserving tea prepared by mixing cistanche and fresh tea leaves and a processing method of the health-preserving tea, and belongs to the technical field of tea processing and health-preserving food. The health-preserving tea is prepared by taking fresh Arabic 3-5-year-old cistanche deserticola (containing more than or equal to 1.2% of echinacoside and more than or equal to 0.8% of verbascoside) and high-quality fresh tea leaves (tea polyphenol is more than or equal to 15%) as raw materials, mixing the raw materials according to a fresh weight ratio of (10-40%): (60-90%), and carrying out processes of pretreatment, mixed rolling, adaptive fixation, segmented drying, fragrance extraction / aging, molding packaging and the like. Through precise raw material ratio, clear component index and process parameter optimization, deep fusion of cistanche health-preserving components and tea flavor is realized, and the problems that pure cistanche tea is heavy in fishy smell and bitter taste and poor in brewing resistance, and common tea is single in health-preserving function and unstable in quality are solved. The product has the double effects of rich tea fragrance, sweet and sweet taste (the score of fishy taste and bitter taste is less than or equal to 2), oxidation resistance and nourishing and conditioning, and the process is compatible with existing tea processing equipment, is low in industrialization threshold, is suitable for multiple consumption scenes, and has remarkable market competitiveness and popularization value.

Provided is an agent for improving dysfunction caused by spinal cord compression, wherein said agent contains at least one selected from the group consisting of Cistanche deserticola, Acteoside, and Echinacoside.

The application discloses a kind of Cistanche multi-field coupling combined drying method and preparation process, it is related to the technical field of traditional Chinese medicinal materials processing, including the way of temperature field, humidity field, microwave field, vacuum field and airflow field five field synergic coupling, design raw material pretreatment, low-temperature air field pre-drying, multi-field coupling main drying, low-temperature vacuum final drying and post-processing ladder type continuous process, realize the rapid, uniform, low-temperature dehydration of Cistanche.The process greatly shortens drying period, reduces unit product energy consumption, can maximize the core active ingredients such as echinacoside, verbascoside in desert Cistanche, the obtained finished product color is uniform, no burnt paste no hard core, rehydration is excellent.The drying efficiency and energy consumption of the application are considered, the ladder type drying design of the process through five field synergic coupling, greatly shortens the drying period of Cistanche, and realizes the efficient use of energy, compared with traditional drying process unit product energy consumption reduces more than 30%, adapts to the cost control demand of industrialized large-scale production.

To provide a permanent wave pretreatment agent, a permanent wave solution, and a permanent wave method that offer excellent wave retention in permanent waves. [Solution] The permanent wave pretreatment agent contains one or more polyphenols selected from chicoric acid, gallic acid, tannic acid, tyriloside, and echinacoside; the permanent wave agent contains one or more polyphenols selected from chicoric acid, gallic acid, tyriloside, echinacoside, and quercetin, and a reducing agent; and the permanent wave method uses the above permanent wave pretreatment agent and / or permanent wave agent.

The invention discloses wine-processed cistanche deserticola as well as a processing technology and application thereof. The preparation method comprises the following steps: removing impurities from a dried cistanche medicinal material, cleaning, softening, slicing, adding yellow wine and Lactobacillus plantarum JY003 viable bacteria, fermenting at 25-35 DEG C for 3-5 hours, steaming, and drying to prepare the wine-processed cistanche decoction pieces. According to the method, Lactobacillus plantarum JY003 with the preservation number of CCTCC NO: M 20252157 is taken as a fermentation engineering bacterium, and the dissolution amount of the core active ingredient echinacoside of the wine-processed cistanche deserticola prepared after fermentation is obviously increased, so that the efficacy of the wine-processed cistanche deserticola is improved, and especially the efficacy related to echinacoside is improved.

This invention discloses a method for detecting a Shenqi Ma Rong Tang composition. The method employs high-performance liquid chromatography (HPLC), including the preparation of a reference solution, a test solution, and a mixed reference solution, as well as HPLC detection steps. By detecting the test solution and the mixed reference solution, a characteristic chromatogram of the Shenqi Ma Rong Tang composition is established. Nine common chromatographic peaks are selected as characteristic peaks, with echinacoside as the reference peak. The relative retention time and relative peak area of ​​each characteristic peak are calculated, and the corresponding components are identified. The components corresponding to the nine characteristic peaks are adenosine, amygdalin, echinacoside, erythritol, verbascoside, gentianin, gentianin, and cassia seed extract. This method can comprehensively reflect the overall chemical characteristics of the Shenqi Ma Rong Tang composition and can be used for its quality control.

The invention discloses a process for affinity separation of active components in inonotus obliquus by using a VEGFR2 extracellular domain and application, and relates to the technical field of active component extraction.The process comprises the steps that eight active components including echinacoside, trilliin, typhaenoside, hederagenin, asiaticoside, lanosterol, betulin and aster ketone are selected; the inhibition effect of the compound on cervical cancer cells is studied. The method comprises the following steps: taking a sample to be detected, taking a detection solution, carrying out lauryl sodium sulfate-polyacrylamide gel electrophoresis and Western blot analysis, determining the protein expression quantity of VEGFR-2 related pathways, and researching the regulating effects of trillioside, betulin, hederagenin and lanosterol on a PI3K / AKT signal pathway, a PLC gamma / ERK signal pathway, Bad protein, Caspase-9 protein and Cleaved Caspase-9 protein of SiHa cells.

The invention relates to the field of gene engineering, in particular to saccharomyces cerevisiae gene engineering bacteria for producing verbascoside and echinacoside through de novo synthesis and application of the saccharomyces cerevisiae gene engineering bacteria. The genetically engineered bacterium contains a coded chorismate synthase mutant, a chorismate mutase mutant, shikimic acid kinase, cytochrome b5, glucose-6-phosphate-dehydrogenase, aromatic acetaldehyde synthase, UDP (User Datagram Protocol) glucosyltransferase, tyrosine ammonia lyase, phenylalanine ammonia lyase, cinnamate hydroxylase, 4-coumaric acid: coenzyme A ligase and acyltransferase. The invention relates to a gene encoding a UDP glucosyltransferase mutant, and more preferably, the gene encoding the UDP glucosyltransferase mutant comprises genes of a UDP glucosyltransferase mutant, a UDP glucosyltransferase mutant, a UDP glucosyltransferase mutant and a UDP glucosyltransferase mutant. Under a shake flask condition, verbascoside produced by the strain reaches 231 mg / L, echinacoside produced by the strain reaches 184 mg / L, and the strain has industrial application potential.

The invention discloses a cistanche Fuzhuan tea beverage as well as a preparation method and a shelf life prediction method thereof, belongs to the technical field of food processing, and mainly solves the problems that in the prior art, Fuzhuan tea prebiotics and cistanche phenylethanoid glycoside active ingredients are low in extraction efficiency, easy to degrade and poor in product stability. Comprising the following steps: respectively carrying out enzymolysis on Fuzhuan tea and cistanche deserticola through a compound enzyme preparation, and combining instantaneous pressure difference treatment to improve the extraction rate; embedding the cistanche deserticola extract by a microcapsule technology, mixing the cistanche deserticola extract with the Fuzhuan tea leach liquor, the sweetening agent and the acidulant, homogenizing, filling and sterilizing to obtain a final product. According to the method, efficient extraction and protection of active ingredients can be achieved, the stability and functionality of the product are remarkably improved, and the method is suitable for developing the cistanche Fuzhuan tea drink with the health-care function. According to the shelf life prediction method, through accelerated test and Arrhenius model fitting, the shelf life of the product is rapidly predicted through echinacoside degradation kinetics.

The application discloses a process for affinity separation of active ingredients in Inonotus obliquus by using an extramembrane region of VEGFR2 and application thereof, and relates to the technical field of active ingredient extraction. Eight active ingredients, namely echinacoside, strophanthin, typhonioside, hederagenin, asiaticoside, lanosterol, betulinol and shionin, are selected to study the inhibiting effect of the active ingredients on cervical cancer cells. The detection liquid is taken to perform sodium dodecyl sulfate-polyacrylamide gel electrophoresis and Western blot analysis, the expression amount of VEGFR-2 related pathway proteins is determined, and the regulation effects of strophanthin, betulinol, hederagenin and lanosterol on the PI3K / AKT signal pathway, the PLC gamma / ERK signal pathway, Bad protein, Caspase-9 protein and Cleaved Caspase-9 protein of SiHa cells are studied.

The invention discloses a cistanche deserticola phenylethanoid glycoside extraction process optimization method. The method comprises the following steps: extracting and preparing a cistanche deserticola phenylethanoid glycoside dry extract; weighing a proper amount of echinacoside reference substance to prepare a standard substance solution, and calculating the content of phenylethanoid glycoside in the desert cistanche deserticola extract by combining the echinacoside standard curve; the method comprises the following steps: determining the content of the extracted cistanche deserticola phenylethanoid glycoside for multiple times by taking the ethanol concentration, the solid-liquid ratio, the extraction temperature, the extraction time and the extraction times of an extracting solution as dependent variables and taking the cistanche deserticola phenylethanoid glycoside yield as a measurement index, and determining the process condition under the maximum extraction amount as the optimal extraction condition; the optimal extraction process is subjected to reproducible operation. According to the method, the optimal process parameters are determined by measuring the content of the cistanche deserticola phenylethanoid glycoside solution and through a uniform design test, and finally, the reproducible operation is performed under the condition of the optimal process parameters, so that the overall optimization is accurate and convenient.

The application discloses application of echinacoside in preparation of a medicine for treating oral squamous cell carcinoma and a medicine composition, relates to the technical field of biological medicine, and discloses, for the first time, that echinacoside (ECH) is applied to treatment of oral squamous cell carcinoma (OSCC), and through network pharmacology, in-vitro cell experiments and in-vivo animal experiments, the significant anti-tumor effect is verified, and a new candidate drug for treatment of OSCC is provided.

The invention discloses application of echinacoside in preparation of an anti-caries agent and an oral care product, and belongs to the technical field of crossing of oral medicine and food science. Under the condition of not influencing the survival of streptococcus mutans, echinacoside can inhibit the biofilm forming ability, acidity production and acid resistance of streptococcus mutans, reduce enamel demineralization, and prevent and delay the development of caries; the echinacoside is derived from medicinal and edible traditional Chinese medicine cistanche, has no obvious influence on oral flora of normal people, and can be used as a medicine for preventing caries and an oral care product.

The invention belongs to the technical field of bioengineering, and particularly relates to a plant lactobacillus XD117, application of the plant lactobacillus XD117 in cistanche fermentation, a cistanche fermentation product and a preparation method of the cistanche fermentation product. According to the preparation method disclosed by the invention, the cistanche is subjected to liquid-state fermentation by adopting the plant lactobacillus XD117, and compared with a cistanche water extract and leavening of other microbial fermentation strains, the prepared leavening has the advantages that on one hand, the echinacoside content and the total saponin content are obviously improved; on the other hand, through an in-vitro simulated digestion test, the biological accessibility of echinacoside and verbascoside is improved, and digestion and absorption of a human body are facilitated. The invention not only provides an effective, simple and convenient cistanche deserticola fermentation method, but also provides a wide foundation for further application of cistanche deserticola.

The present application relates to a kind of application of echinacoside in the preparation of anti-liver senescence drugs, belong to the technical field of medicine.The present application is found through small molecule drug screening, echinacoside is combined with WDR6 protein that plays an important role in resisting aging in liver.Echinacoside plays the role of improving liver senescence phenotype by being combined with liver WDR6 protein.The present application is verified through in vivo and in vitro experiment, echinacoside can improve age-related serum albumin decrease by being combined with liver WDR6 protein, reduce the number of senescent cells in liver tissue, and delay the liver fibrosis process intensified with age.The detection of molecular mechanism level finds that the long-term administration of echinacoside improves the down-regulation of longevin SIRT1 protein in senescent liver, and inhibits the expression of senescence marker proteins p21 and p16.