26 results about "Amygdalin" patented technology

The invention discloses wine peach kernel decoction pieces and a preparation method thereof, and the method comprises the following steps: adding 10-20% by mass of yellow wine into peeled and cleaned peach kernels, uniformly stirring, and moistening at room temperature for 20-40 minutes to enable the yellow wine to be soaked and absorbed; and placing the moistened peach kernels in a drum-type herbal medicine roaster, frying at 100-140 DEG C for 10-20 minutes until the water content is 3-12%, and cooling to obtain the wine peach kernel decoction pieces with wine aroma. The wine peach kernel decoction pieces prepared by the method disclosed by the invention have the extract content of 21-31% and the amygdalin content of 2.4-3.6%, and are suitable for large-scale stable production.

The invention relates to a traditional Chinese medicine composition which comprises more than 1.09 permillage of ephedrine hydrochloride, more than 0.86 permillage of pseudoephedrine hydrochloride, more than 0.17 permillage of peiminine, more than 0.03 permillage of peimine, more than 0.17 permillage of chrysin-7-O-glucuronide, more than 0.002 permillage of glycyrrhetinic acid, more than 11.87 permillage of amygdalin and more than 0.01 permillage of neotuberostemonine. The oral liquid is prepared from more than 2.16 per mill of glycyrrhizic acid, more than 1.97 per mill of oroxylin A, more than 1.97 per mill of oroxylin B, more than 1.23 per mill of liquiritin, more than 0.59 per mill of apiose liquiritin, more than 0.55 per mill of baicalin, more than 0.23 per mill of chlorogenic acid, more than 0.18 per mill of praeruptorin A, more than 0.15 per mill of wild melanosin, more than 0.14 per mill of methyephedrine hydrochloride, more than 0.13 per mill of isoliquiritin, more than 0.13 per mill of neochlorogenic acid and more than 0.13 per mill of cryptochlorogenic acid. The composition is prepared from more than 0.10% of peimine, more than 0.08% of baicalein, more than 0.05% of praeruptorin B, more than 0.04% of praeruptorin C, more than 0.011% of xanthoxylin and more than 0.0006% of formononetin.

The application provides a preparation method of a semi-solid pure loquat whole fruit wine, comprising separation of loquat pulp and loquat kernel, loquat pulp beating, loquat kernel processing, mixing, fermentation and distillation. The application makes full use of rich carbon sources such as starch in loquat kernel and the amygdalin component with the effect of relieving cough, utilizes the aroma and nutrition of loquat fresh fruit, and absorbs the medicinal active components of loquat whole fruit. The semi-solid fermentation mode is adopted to produce the loquat whole fruit wine containing the antioxidant and cough-relieving medicinal active components, realizes efficient and high-value utilization of loquat resources, and the produced loquat whole fruit wine has a high yield and a unique loquat fragrance.

The application discloses a method for lime-free and acid-free copper-sulfur flotation separation, wherein a combined inhibitor for copper-sulfur separation comprises ferrous sulfate and amygdalin, and is used as a pyrite inhibitor in flotation separation of chalcopyrite and pyrite.The combined inhibitor ferrous sulfate and amygdalin, a collector and a foaming agent are added to a raw ore after crushing and grinding to perform a copper flotation operation, and after the copper flotation operation, a combined activator sodium percarbonate and copper sulfate, a combined collector and a foaming agent are added to the ore pulp to perform a sulfur flotation operation.The combined inhibitor ferrous sulfate and amygdalin has a strong inhibiting effect on pyrite, and can effectively realize flotation of chalcopyrite; the combined activator sodium percarbonate and copper sulfate has a good de-inhibiting and activating effect on pyrite, and can effectively realize flotation of pyrite, thereby reducing the mineral processing cost while enhancing the copper-sulfur separation effect, and having a good application prospect.

This invention belongs to the technical field of qualitative determination of ephedra, licorice, and bitter almond in traditional Chinese medicine compound preparations. This invention discloses a method for simultaneously identifying ephedra, licorice, and bitter almond in Ma Xing Shi Gan oral liquid. The test sample is placed in a Soxhlet extractor and treated with reagents of different properties to prepare a fractional extraction solution. Ephedra and licorice reference materials are then processed to obtain a reference material solution. Amygdalin reference standard is prepared by adding methanol to prepare a reference solution. The above test sample solution, reference material solution, and reference solution are used in a thin-layer chromatography system to simultaneously identify ephedra, licorice, and bitter almond. The results show that the chromatographic spots are well separated and clear, with no interference from the negative control. This method has simple pretreatment, is environmentally friendly, has high sensitivity, and its repeatability and robustness meet the requirements of the Chinese Veterinary Pharmacopoeia, thus enabling better quality control of Ma Xing Shi Gan oral liquid.

The goal is to artificially ripen unripe green plums in a short period of time, thereby providing plums that are equivalent in quality to naturally ripened, fully mature plums. [Solution] In the decomposition step S4, the decomposition of cyanogenic glycosides such as amygdalin contained in the pulp of raw plums promotes the production of hydrogen cyanide and benzaldehyde. In the detoxification step S5, the produced hydrogen cyanide and benzaldehyde are detoxified. In the high-temperature sterilization step S7, the detoxified plums are sterilized at high temperature. In the ultraviolet irradiation step S8, the plums are further ripened by ultraviolet irradiation. As a result, unripe green plums can be artificially ripened in a short period of time, such as a few days, and plums equivalent to naturally ripened yellow plums that mature over about a month can be obtained.

The application discloses a mandelonitrile-degrading bacterium SYY40 and a culture method and application thereof, relates to the technical field of microbial pollution degradation and environmental pollution, and provides the mandelonitrile-degrading bacterium SYY40 which is identified as Pseudomonas sp. SYY40 and named Pseudomonas sp. SYY40, and is preserved in the China General Microbiological Culture Collection Center on May 19, 2025, and the preservation number of the strain is CGMCC NO. 34596. The strain can realize efficient removal of mandelonitrile, and the degradation rate of the mandelonitrile reaches 100% within 48 hours.

The invention relates to a synthetic flora for degrading amygdalin and application of the synthetic flora in relieving amygdalin stress of peach trees. Through rhizosphere microorganism screening and functional verification, a strain, namely Sinomonas A7, which is enriched under the stress of mandelate in peach seedlings and has efficient degradation capability is identified, and the strain, Pristia megaterium C8 and Caballaria turbo P16 jointly form a synthetic flora with a synergistic effect. Experimental results show that the synthetic flora can effectively relieve the amygdalin-mediated autotoxicity effect of the plants, enhance the tolerance of the plants to stress and promote growth. The invention provides a technical route of the multifunctional complex microbial inoculant, and the multifunctional complex microbial inoculant has potential application values of removing autotoxic substances and improving crop resistance and productivity, and is suitable for rhizosphere improvement and health management of peach tree seedlings.

The invention discloses a gene related to synthesis of amygdalin from crop seed endosperm and application of the gene, and belongs to the technical field of gene engineering. According to the invention, gene sequences of CYP79D16, CYP71AN24, UGT94AF3 and UGT94AF2 are optimized, CYP79D16 and CYP71AN24 are connected by using a linker peptide I to obtain a fusion gene I, and the fusion gene I is fused with a breeding crop endosperm strong expression promoter to construct a gene expression cassette I; connecting UGT94AF3 and UGT94AF2 by using a connecting peptide II to obtain a fusion gene II, and fusing the fusion gene II with a breeding crop endosperm strong expression promoter to construct a gene expression cassette II; transferring the gene expression cassette into an expression vector to construct a recombinant vector, and introducing the recombinant vector into a plant to obtain a transgenic crop. According to the invention, synthesis of amygdalin in crop seed endosperm is realized, the storability of crop seeds is improved, and the product can be used as a raw material for producing amygdalin, and has guiding significance and production application value for crop breeding.

The invention discloses a method for detecting effective components of a cervicitis treatment composition, which adopts high performance liquid chromatography for detection, and the chromatographic conditions are as follows: a chromatographic column is a C18 chromatographic column; the detection wavelength is 207 nm; a mobile phase A is a 0.2% phosphoric acid aqueous solution, a mobile phase B is acetonitrile, gradient elution is adopted, the mobile phase proportion is volume percent, and the elution procedure is as follows: 0-5 min, the mobile phase A is 100%, and the mobile phase B is 0%; the mobile phase A accounts for 100%-96% and the mobile phase B accounts for 0%-4% in 5-40 min; 40-60 min, the mobile phase A is 88%-58%, and the mobile phase B is 12%-42%; the mobile phase A accounts for 92% and the mobile phase B accounts for 8% in 60-80 min. The method disclosed by the invention can be used for simultaneously determining the components such as sophocarpidine, sophoridine, oxymatrine and amygdalin in the cervicitis composition, is convenient to operate, accurate in detection result and good in stability, and has very high practicability.

The application discloses a kind of shentong zhuyu decoction fingerprint construction method and application, belong to the technical field of drug detection.The application is detected by high performance liquid chromatography to several batches of shentong zhuyu decoction by the selection of mobile phase and the cooperation of chromatographic column, the high performance liquid chromatography of different batches of shentong zhuyu decoction is compared, obtains the common peak containing loganic acid, amygdalin, hydroxyl safflower yellow pigment A, aniseed acid, qianghuo alcohol, isoeucommarin, ammonium glycyrrhizinate, kaempferol characteristic peak, and the fingerprint of shentong zhuyu decoction is constructed according to the common peak.The fingerprint constructed by the application can better detect 12 medicinal materials, shorten the detection time of single sample to 125 min or less, improve efficiency, and improve the quality control level of product.

The application discloses a method for establishing HPLC fingerprint spectrum of Lianhuaqingwen capsules based on chemical identification method, and is characterized in that the method adopts high performance liquid chromatography and specifically comprises the following steps: 1) preparation of reference solution: precisely take new chlorogenic acid, quercitrin, forsythia glycoside, amygdalin, emodin, chrysophanol, chlorogenic acid, forsythia glycoside A, rhein, glycyrrhizic acid reference, and add methanol to prepare a reference solution; 2) preparation of test sample solution: 3) chromatographic conditions: the mobile phase is 0.1% phosphoric acid aqueous solution (A)-acetonitrile (B), gradient elution; the detection wavelength is 207 nm, the column temperature is 30 DEG C, and the flow rate is 1.0 mL·min ‑1 ; 4) determination: precisely take the test sample solution and inject it into a high performance liquid chromatograph, and the HPLC fingerprint spectrum is obtained by HPLC determination. The HPLC fingerprint spectrum established by the application is helpful for further improving the quality evaluation system of Lianhuaqingwen capsules and can provide a reference for the quality control of Lianhuaqingwen capsules.

The present application relates to a kind of amygdalin hapten, hybridoma cell strain, antibody and application, belong to the field of immunochemistry.The hybridoma cell strain DMN has been preserved in China Microbial Culture Collection Center CGMCC, address Beijing Chaoyang District Beichen West Road No. 1 Yard No. 3, Institute of Microbiology, Chinese Academy of Sciences, preservation date is April 18, 2024, and the preservation number is CGMCC No.45923.The monoclonal antibody secreted by the cell strain provided in the present application has good detection sensitivity (IC 50 Values are 8.65 ng / mL) and specificity, which can realize the rapid detection of amygdalin, provide raw materials for the immunodetection of amygdalin in amygdalin and products, and have practical application value.

This invention discloses a method for detecting a Shenqi Ma Rong Tang composition. The method employs high-performance liquid chromatography (HPLC), including the preparation of a reference solution, a test solution, and a mixed reference solution, as well as HPLC detection steps. By detecting the test solution and the mixed reference solution, a characteristic chromatogram of the Shenqi Ma Rong Tang composition is established. Nine common chromatographic peaks are selected as characteristic peaks, with echinacoside as the reference peak. The relative retention time and relative peak area of ​​each characteristic peak are calculated, and the corresponding components are identified. The components corresponding to the nine characteristic peaks are adenosine, amygdalin, echinacoside, erythritol, verbascoside, gentianin, gentianin, and cassia seed extract. This method can comprehensively reflect the overall chemical characteristics of the Shenqi Ma Rong Tang composition and can be used for its quality control.

The application discloses a kind of amygdalin / liquorice acid self-assembled hydrogel and its preparation method and application, preparation method includes the following steps: amygdalin solution and liquorice acid are mixed and dispersed uniformly, stationary, obtain amygdalin / liquorice acid self-assembled hydrogel;The concentration of the amygdalin solution is 0.015~0.040g / mL, and the concentration of liquorice acid in amygdalin / liquorice acid self-assembled hydrogel is 0.015~0.040g / mL.Amygdalin / liquorice acid self-assembled hydrogel microstructure presents three-dimensional porous network structure, can be used to prepare drug for reducing or treating inflammatory encephalopathy, neuron damage encephalopathy, blood-brain barrier destruction disease, complement activation disease, the preparation of the application is simple, safe and aimed at complement activation inhibition.

The invention relates to the field of quality control of traditional Chinese medicine preparations, in particular to a construction method of an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of Xiangyanping granules and a quantitative detection method of seven components. According to the method, an HPLC (High Performance Liquid Chromatography) one-needle multi-detection method is adopted, a plurality of index components are detected at the same time by optimizing a gradient elution program and multi-wavelength scanning (212 nm / 236 nm / 283 nm / 326 nm), synchronous quantification of various active components such as amygdalin, paeoniflorin, ferulic acid, hesperidin and chlorogenic acid is realized, the efficiency is improved, and the cost is saved. The established method has the advantages of strong specificity, high precision, good durability and good repeatability, and can be used for quality control of Xiangyanping granules.

The application discloses a characteristic spectrum method for Juhong Tanke preparation and application, wherein (1) the preparation of the test sample solution: the Juhong Tanke preparation is weighed, dissolved in a 50% methanol solution, filtered, and the filtrate is obtained; (2) determination: the test sample solution is taken and injected into a high performance liquid chromatograph for determination, so as to obtain the characteristic spectrum of the Juhong Tanke preparation. The application first establishes a detection method which can simultaneously identify four main medicinal materials in the Juhong Tanke preparation. The characteristic spectrum contains seven characteristic peaks, corresponding to amygdalin, naringin, wild lacquer tree glycoside, orange peel lactone hydrate, isoscutellarein, schisandrol A and a component derived from licorice. Four characteristic components belong to the Juhong Tanke preparation, one characteristic component belongs to the bitter almond, one characteristic component belongs to the Schisandra chinensis, and one characteristic component belongs to the licorice. Through the characterization of the multi-medicinal material components, the system comprehensive evaluation of the overall quality of the Juhong Tanke preparation is realized.

The invention relates to a traditional Chinese medicine composition which is prepared from active ingredients extracted from 1-3 parts of roasted ephedra, 1-10 parts of semen armeniacae amarae, 1-6 parts of platycodon grandiflorum, 1-6 parts of radix peucedani, 1-6 parts of thunberg fritillary bulb, 1-6 parts of radix stemonae, 1-3 parts of oroxylum indicum, 1-10 parts of radix glehniae and 1-3 parts of liquorice in percentage by mass as follows: more than 1.09% o of ephedrine hydrochloride, more than 0.86% o of pseudoephedrine hydrochloride and the balance of water. The traditional Chinese medicine composition is prepared from more than 0.17 per mill of peiminine, more than 0.03 per mill of peimine, more than 0.17 per mill of chrysin-7-O-glucuronide, more than 0.002 per mill of glycyrrhetinic acid, more than 11.87 per mill of amygdalin and more than 0.01 per mill of neotuberostemonine.

The invention discloses a glycoside sterol component lipid nanoparticle for regulating and controlling mRNA transfection as well as a preparation method and application of the glycoside sterol component lipid nanoparticle, and belongs to the technical field of pharmaceutical preparation preparation. The nanoparticle is prepared from DLin-MC3-DMA, DOPE, different glycosides or sterols and DSPE-PEG-GSH. The different glycosides or sterols comprise amygdalin Amy, beta-sitosterol, stigmasterol Sti, ginsenoside Rg1 and salidroside Sal. Stigmasterol can improve enrichment of LNP in the spleen, ginsenoside can adjust immunity and enhance the anti-tumor immune effect, the preparation method is simple, needed equipment is conventional equipment, large-scale production can be achieved, and therefore the stigmasterol and ginsenoside compound has good industrial application value and market prospects.

This invention discloses a pharmaceutical composition for treating acute soft tissue injuries. Its active ingredients consist of geniposide and amygdalin in a mass ratio of 1:1-3, preferably 1:2. It can be prepared as a gel or other topical formulation and contains pharmaceutically acceptable excipients. This pharmaceutical composition is developed based on traditional sprain powder. Through the synergistic effect of geniposide and amygdalin, its therapeutic effect is significantly superior to sprain powder and single-component formulations, effectively improving symptoms such as swelling and bruising at the injury site. Its components are clearly defined, and its quality is easily controlled, overcoming the shortcomings of traditional Chinese medicine compound formulas, which are characterized by complex components and inconsistent quality. Furthermore, topical administration causes no gastrointestinal irritation, making it suitable for a wide range of patients and applicable to the preparation of drugs for treating acute soft tissue injuries.

The invention discloses an application of amygdalin in preparation of a medicine for preventing and / or treating non-small cell lung cancer, and belongs to the technical field of biological medicines. It is found for the first time that the natural compound amygdalin can effectively inhibit growth of EGFR mutant lung cancer by promoting lysosome degradation of EGFR protein and inhibiting downstream signal channels of the EGFR protein; in addition, the C797S mutation-mediated tyrosine kinase inhibitor has a remarkable treatment potential on the drug resistance of the C797S mutation-mediated tyrosine kinase inhibitor. In-vivo research further proves that the combination of amygdalin and osimertinib can generate a synergistic effect and enhance the anti-tumor curative effect. It is worth noting that the amygdalin has no obvious cytotoxicity under the effective action concentration and has good safety. Results show that the amygdalin is expected to become a novel EGFR targeted degradation agent, and a new treatment strategy is provided for non-small cell lung cancer patients with tyrosine kinase inhibitor drug resistance.

The present application belongs to the preparation method of hydrocyanic acid, and relates to a method for enriching hydrocyanic acid from kernel apricot thinning fruit, which comprises the following steps: S1, softening; S2, crushing-decomposition-pressing: the kernel apricot thinning fruit after softening is crushed by adding water, then is placed to make amygdalin in the kernel apricot thinning fruit decompose to generate hydrocyanic acid, and then is pressed, filtered and the filtrate is collected; S3, freezing concentration: the filtrate of step S2 is subjected to freezing concentration under ultrasonic vacuum condition, and thinning fruit juice concentrate is obtained; S4, distillation recovery. The present application takes kernel apricot thinning fruit as raw material, and mainly enriches high-concentration hydrocyanic acid product through the processes of softening, crushing-decomposition-pressing, freezing concentration and distillation recovery. The method of the present application has relatively mild reaction condition, and the reaction yield of hydrocyanic acid is high; no chemical reagent is added, and the method is green and environment-friendly.

This invention relates to the field of biomedicine, specifically to a DNA ligase III (LIG3) inhibitor and its applications. The inhibitor contains amygdalin, with the chemical formula C0. 29 H 30 O 13 The structure is that of an inhibitor that can cause DNA damage and apoptosis in tumor cells and can be used to treat breast cancer, but not limited to breast cancer tumors and related diseases.

The invention discloses a liquid chromatography-mass spectrometry detection method of bulbus fritillariae cirrhosae lung-heat-clearing syrup, which comprises the following steps: respectively taking a reference substance and the bulbus fritillariae cirrhosae lung-heat-clearing syrup, adding methanol for dissolving to obtain a reference substance solution and a test solution, and injecting the reference substance solution and the test solution into an HPLC-QQQ / MS liquid chromatography-mass spectrometer for detection. The detection method disclosed by the invention can be used for detecting rehmannia glutinosa-D, catalpol, amygdalin, platycodin-D, ophiopogonin-C and ophiopogonin-D in the fritillaria cirrhosa lung-heat-clearing syrup, is used for identifying active ingredients in the fritillaria cirrhosa lung-heat-clearing syrup, and perfects the quality control standard of the fritillaria cirrhosa lung-heat-clearing syrup. According to methodology verification, the detection method is high in accuracy and sensitivity, can realize specific detection of the characteristic components of the bulbus fritillariae cirrhosae lung-heat-clearing syrup, and has practical application value.

The invention provides a construction method of a children cough specific chromatogram based on a UPLC (Ultra Performance Liquid Chromatography) instrument. The children cough specific chromatogram established based on the UPLC method has eight common peaks, and the specified values are as follows: the oleanolic acid is 0.34 (peak 1), the ephedrine hydrochloride is 0.38 (peak 2), the pseudoephedrine hydrochloride is 0.42 (peak 3), the amygdalin is 0.54 (peak 4), the liquiritin is 1 (peak 5), the peak 6 is 1.36 (peak 6), the praeruptorin A is 1.64 (peak 7), and the ammonium glycyrrhizinate is 1.82 (peak 8). According to the invention, verification of precision, repeatability and stability experiments is carried out on the constructed children cough specific chromatogram method, the RSD value of the relative retention time of each peak in each experiment result is less than or equal to 1.0%, and the RSD value of the relative peak area is less than or equal to 5.0%. The specific chromatogram method provided by the invention is good, and the method can reflect various chemical components in the children cough liquid; moreover, the detection time is only 10 minutes per sample, and the overall quality of the children cough liquid can be controlled more efficiently and quickly.

The invention relates to a traditional Chinese medicine composition, which is an active component extracted from cassia twig, poria cocos, moutan bark, peach kernel and white paeony root, and the active component comprises the following components in percentage by mass: more than 0.214% of gallic acid, more than 0.008% of 4-hydroxybenzoic acid, more than 0.083% of oxypaeoniflorin, more than 1.112% of paeoniflorin, more than 0.012% of sodium hyaluronate and more than 0.012% of sodium hyaluronate. The content of ethyl gallate is more than 0.010%, the content of 1, 2, 3, 4, 6-O-pentagalloylglucose is more than 0.155%, the content of benzoic acid is more than 0.041%, the content of cinnamic acid is more than 0.015%, the content of benzoylpaeoniflorin is more than 0.093%, the content of cinnamaldehyde is more than 0.037%, the content of paeonol is more than 0.622%, and the content of amygdalin is more than 1.001%. The content of large-class components, namely water-soluble polysaccharide, is 1.743% or above, the content of alkali-soluble polysaccharide is 29.671% or above, and the total content of monosaccharide and disaccharide is 9.231% or above.