195 results about "Amygdalin" patented technology

The invention belongs to the technical field of traditional Chinese medicine formula granules and particularly relates to semen armeniacae amarae formula granules as well as preparation and quality control methods thereof. The L-amygdalin and D-amygdalin ratio range considering both the curative effect and safety (low toxicity) is determined through study on the ratio of L-amygdalin to D-amygdalin in classical prescription decoction containing semen armeniacae amarae, semen armeniacae amarae formula granule extraction process parameters capable of guaranteeing the L-amygdalin and D-amygdalin ratio range are determined through process purification, high performance liquid chromatography is used for quantitative analysis in the quality control method, the amygdalin isomerization course is controlled by regulating process parameters such as decoction water amount and decoction time for semen armeniacae amarae decoction pieces during preparation, the semen armeniacae amarae formula granules meeting the quality requirement are prepared and has the same effect with classical prescriptions, and quality control of the semen armeniacae amarae formula granules is realized.

The invention relates to a rapid separation liquid chromatography detection method for naoxintong capsules. The method comprises the chromatographic conditions that octadecyl bonded silica gel columns serve as filling agents, 0.1% formic acid water serves as an A mobile phase, acetonitrile serves as a B mobile phase, the volume ratio of the A mobile phase to the B mobile phase is 10-85:15-90, and linear gradient elution is performed, the flow velocity is set at 0.20 mL.min<-1>, the column temperature is set at 25 DEG C, and the sample introduction volume is 5 microliters. The content of gallic acid, tanshinol, hydroxysafflor yellow A, chlorogenic acid, amygdalin, protocatechualdehyde, epicatechin, caffeic acid, albiflorin std, ononin, paeoniflorin, rutin, salvianolic acid A, cinnamic acid, fermlononetin, dihydrotanshinone I in the naoxintong capsules is detected. The detection method has the advantages of being rapid, stable and accurate.

The invention provides a method for separating the purified amygdalin from the loquat nucleus, comprising: extracting the amygdalin from the loquat nucleus to produce a concrete with a hydrophilic organic water solution; fully dissolving the concrete by ethanol, filtering, depressed dealcoholization of the filtrate, cryogenic crystallization to produce the coarse amygdalin; fully dissolving the amygdalin, filtering, cryogenic recrystallization, washing by ethanol-aether, getting a amygdalin white crystal with a purity not less than 98.7%. The invention purifies the amygdalin from the loquat nucleus which solves the problem that the amygdalin as an original medicine is hydrolyzed by acid and enzyme so as to reduce the curative effect and has a broad extension.

The invention provides a new preparation method and a quality control method of a pill containing cassia twig and tuckahoe, The preparation method have the advantages of few production steps, short production cycle, high production mechanization, stable preparation quality, high content of effective ingredients, fast disintegration, high bioavailability, small dose, convenient administration and the like. The invention also provides the quality control method of cinnamyl aldehyde, cinnamic acid, paeonol, peoniflorin and amygdalin in the pill containing cassia twig and tuckahoe.

The invention provides a content detection method for determining effective components in a longshengzhi capsule by an HPLC-QQQ/MS method. The chromatograph condition is that the chromatographic column takes a octadecyl-bonded silica gel column as a filling agent, 0.1% formic acid-water as a mobile phase A, 0.1% formic acid-acetonitrile as a mobile phase B, and the volume ratio of the mobile phaseA and the mobile phase B is 80% to 0%:20 to 100%, so that gradient elution is performed. According to the content detection method for determining the effective components in the longshengzhi capsuleby the HPLC-QQQ/MS method, the effective substance components of traditional Chinese medicines such as astragaloside A, hydroxysafflor yellow A, calycosin 7-o-glucoside, calycosin, ferulic Acid, syringin, n-butylphthalide, ligustilide, senkyunolide A, senkyunolide I, senkyunolide H, ligustrazine, isofraxidin, dehydrocostus lactone, amygdalin, syringin E, paeoniflorin, oxypaeoniflora, benzoylpaeoniflorin, etc., in the longshengzhi capsule can be determined. The method has the advantages of being simple in operation method, high in sensitivity and accurate in content.

The invention provides a preparation process and a quality control method of a cassia twig poria cocos preparation. The preparation method has the advantages of less production steps, short production period, high production mechanical degree, stable quality of the preparation, high content of effective components, rapid disintegration, high bioavailability, small dose oral administration, convenience in oral administration and the like. The invention also provides a quality control method of cinnamaldehyde, cinnamic acid, paeonol, paeoniflorin and amygdalin in a cassia twig poria cocos pill.

The invention discloses a method for determining content of effective ingredient amygdalin in loquat leaf cough syrup. The method comprises a step of preparation of reference solution, a step of preparation of test solution and a step of high performance liquid chromatographic determination, wherein the condition that content of amygdalin is not less than 1.2mg per 1ml of the loquat leaf cough syrup can be ensured. According to the method, high performance liquid chromatography is used for determining content of the amygdalin controlling quality of loquat leaf cough syrup product, compared with the detection without content determination item in the existing standard, the method has the advantages that the inherent quality of medicine can be directly controlled by determining content of the amygdalin, moreover, in the determination method, the amygdalin is separated well, stability and accuracy both are high and repeatability is excellent, thus, quality of the loquat leaf cough syrup product can be controlled better.

The invention belongs to the technical field of Chinese patent medicines, and relates to a quality control method for a Xuefuzhuyu capsule prepared from traditional Chinese medicinal materials. In the quality control method, Chinese thorowax root and licorice root are utilized as comparison medicinal materials, and whether the formula of the Xuefuzhuyu capsule contains Chinese thorowax root and licorice root components is identified by means of thin-layer chromatography; and amygdalin (c20h27no11), paeoniflorin (c23h28o11) and naringin (c27h32o14) are used as comparison products, and the contents of the amygdalin, paeoniflorin and naringin in the formula of the Xuefuzhuyu capsule are determined by means of high performance liquid chromatography. By utilizing the quality control method provided by the invention, the situation in the original standards that the quality of compound prescription is controlled by controlling the content of a single indicator component is changed, and the controllability of the quality standards of the Xuefuzhuyu capsule is improved, so as to further ensure the inherent quality and curative effect of products; and the revised quality standards are improved, and the quality control of the Xuefuzhuyu capsule is improved.

The invention belongs to the technical field of Chinese patent medicines, and relates to a quality control method for a Chinese medicine preparation Xuefuzhuyu capsule prepared from traditional Chinese medicinal materials. In the quality control method, citrus trifoliata is utilized as a comparison medicinal material, naringin is used as a comparison product, Chinese angelica and szechwan lovage rhizome are used as comparison medicinal materials, Chinese thorowax root is used as a comparison medicinal material, liquorice is used as a comparison medicinal material, and whether the formula of the Xuefuzhuyu capsule contains citrus trifoliata, Chinese angelica szechwan lovage rhizome, Chinese thorowax root and liquorice is identified by means of thin-layer chromatography; and amygdalin is used as a comparison product, and whether peach seed is contained in the Xuefuzhuyu capsule is identified by means of high performance liquid chromatography. By utilizing the quality control method provided by the invention, fraudulent medicaments and inferior medicaments can be identified more accurately, so as to improve the safety in use. By utilizing an inspection method for determining the content of paeoniflorin used as a quantitative indicator by means of high performance liquid chromatography, the controllability of the quality standards of the Xuefuzhuyu capsule is improved, and the inherent quality and curative effect of products are further ensured; and the revised quality standards are improved, and the quality control of the Xuefuzhuyu capsule is improved.

The invention belongs to the technical field of medical food, and discloses an extraction and purification method of amygdalin in Cerasus humilis kernel. The adopted purification method is simple and effective and can extract the amygdalin in Cerasus humilis kernel to the largest extent. The obtained amygdalin not only can be used for relieving cough and resisting inflammatory, but also can be used for treating tumor, thus the method of the invention has wide application prospect in the pharmaceutical field.

The invention relates to an extraction method for laetrile. Hypercritical carbon dioxide is adopted to be added with an absolute ethyl alcohol entraining agent; in hypercritical carbon dioxide equipment, extraction is carried out to bitter almond meal after degreasing in an extraction kettle; then primary separation, secondary separation and low-temperature crystallization are carried out, and a crude product of the laetrile is obtained. After the crude product of the laetrile is dissolved by using absolute ethyl alcohol secondarily, the temperature is reduced to minus 3 DEG C to 4 DEG C, thus obtaining a fine product of the laetrile. The extraction method for the laetrile has simple and convenient extraction procedure, short extraction time, target products with high yield and low overall cost; the products do not have harmful residual solvents.

The invention relates to a method for extracting and purifying amygdalin from peach seeds. The invention provides a method for extracting and purifying the amygdalin by combining a method for heat extraction by use of an ethanol solution with a method for absorption and desorption by use of macroporous absorption resin, and the method has a remarkable advantage that the purity of the obtained amygdalin is higher (more than 60%). Furthermore, the method for extraction and purification provided by the invention further has the advantages of low cost, environment friendliness, easiness in mass production and the like.

The invention discloses a method for preparing a bitter almond extract product. The method provided by the invention comprises the following steps of: 1) drying bitter almond until the water activity of bitter almond reaches 0.10-0.70; 2) crushing to 10-120 meshes; 3) extracting bitter almond oil on the basis of maintaining the water activity unchanged; 4) separating and extracting bitter almond meal by using an ethanol aqueous solution at the concentration of 50-95% to obtain amygdalin and bitter almond proteins. When crushing bitter almond and extracting bitter almond oil by the adoption ofthe method, amygdalase activity is reduced by controlling the water activity; and during the separation and extraction of the amygdalin and bitter almond proteins, the ethanol concentration and the operation temperature are controlled to reduce the amygdalase activity and prevent the bitter almond protein from overdenaturation. Therefore, the method provided by the invention is used to realize the full utilization of bitter almond oil, bitter almond protein and amygdalin and lay a foundation for the refined and deep processing and comprehensive utilization of bitter almond.

The invention provides a method for controlling the quality of Xingsu cough syrup. The method mainly comprises part or all of identification and content determination items, wherein the identification comprises thin-layer chromatography identification of perilla leaves, balloon flower, ephedra, thunberg fritillary bulb and baikal skullcap root; and the content determination means to determine the content of the main active ingredient amygdalin of bitter almonds in a preparation by adopting a high-performance liquid chromatography, and method validation is carried out, so that a reliable and accurate quality control method with strong specificity is established. By adopting the method, the quality control standard of the Xingsu cough syrup is improved, the quality of a product can be effectively controlled, and then the clinical curative effect of the product is definitely ensured.

The invention discloses a preparation method of D-amygdalin and/or neoamygdalin; the amygdalin is extracted from peach kernels or bitter apricot kernels, an organic solvent is used for extraction, chromatography is used for separating D-amygdalin and neoamygdalin isomers and for detecting the purity of the two isomers, and nuclear magnetic resonance is used for detecting the structures of the two isomers. By adoption of the reverse chromatography technique and combination of the NMR technology, high-precision separation preparation of arbitrary one epimer of D-amygdalin and/or neoamygdalin of the amygdalin is realized. The separation operation process is simple, easy to operate and easy to repeat, the separation cycle is short, and the efficiency is high; moreover, the method is low in cost, friendly to the environment, and suitable for large-scale industrialized production; and in addition, the purity and the yield of the prepared D-amygdalin and neoamygdalin are higher.

The invention provides a detection method of cassiabarktree twig tuckahoe capsules. The method comprises the steps that: solid powder in the cassiabarktree twig tuckahoe capsules is adopted as a sample requiring detection; the sample requiring detection is detected by using a near-infrared spectrometer, so that near infra-red spectrum data of the sample is obtained; according to correction models of gallic acid, peoniflorin, benzoic acid, benzoylpaeoniflorin, cinnamaldehyde, paeonol, and amygdalin, and the obtained near infra-red spectrum data, the contents of gallic acid, peoniflorin, benzoic acid, benzoylpaeoniflorin, cinnamaldehyde, paeonol, and amygdalin in the sample are obtained. According to the invention, the sample requiring detection does not need complicated pretreatments such as separation and extraction. The detection time is shortened, the detection efficiency is improved, and work load is reduced. Also, when the detection method provided by the invention is adopted in detections upon large batches of samples, the result is accurate, and the repeatability is good.

The invention discloses a preparation method of bacterial cellulose. The method comprises the following steps of: preparing a soybean residue biological fermentation culture medium by using soybean residues as a basic raw material, namely soybean residues for short, adding 10-18% of a gluconacetobacter xylinum seed solution and amygdalin into the soybean residue biological fermentation culture medium, adding 12-25 mL of a gluconacetobacter xylinum seed solution into every 100 mL of the soybean residue biological fermentation culture medium, wherein the pH is 4.8-6.0, the culture temperature is 28-35 DEG C, the culture time is 12-18 days, the use amount of amygdalin is 150-450 U/mL, and obtaining a bacterial cellulose membrane material. The invention further discloses a bacterial cellulose-chitosan composite gel skin care mask and a preparation method thereof. The mask is good in tensile strength and high in mechanical strength, has a certain inhibition effect on bacteria and fungi, and is good in biocompatibility.

The invention provides a medicated leaven fermentation process on-line quality detection system. A method comprises a step a of obtaining liquid chromatography data of each sample by adopting high performance liquid chromatography; respectively determining contents of artemisinin, quercetin, rutin and amygdalin in each sample; a step c of performing protease enzyme activity prediction: y=0.0021x1+3.1067x2-1.4643x3+0.0116x4+0.2095x5+27.6106; performing amylase enzyme activity prediction: y=0.3054x1+89.2468x2-16.0689x3-0.0748x4+0.2888x5+352.1262, wherein x1 is artemisinin, x2 is quercetin, x3 is rutin, x4 is amygdalin, x5 is fermentation time, and y is protease and/or amylase enzyme activity. The medicated leaven fermentation process on-line quality detection system is favorable for enabling users to learn about dynamic changes in fermentation process intermediate product quality in real time and favorable for optimizing intermediate product quality development direction through process parameter control, and thus the quality pass rate of a final product is improved.

The invention provides a method for quickly identifying authenticity and quality of semen armeniacae amarae based on a hyperspectral imaging technology, the method comprises the following steps: collecting hyperspectral images of semen armeniacae amarae samples of different producing areas and semen armeniacae amarae samples of easy-to-confuse varieties, and combining and comparing in pairs based on different preprocessing methods and classification models to realize classification and authenticity identification of semen armeniacae amarae medicinal materials of different producing areas; further based on a characteristic wave band extraction method of the amygdalin content, establishing a prediction model of the amygdalin content in the bitter apricot kernel medicinal material, and realizing quality identification of the bitter apricot kernel medicinal material is realized; according to the method, the quality of semen armeniacae amarae is comprehensively evaluated from inside to outside based on the hyperspectral imaging technology, a quick and lossless analysis method is provided for selection and identification of high-quality semen armeniacae amarae medicinal materials, and technical support is provided for industrial intelligent control of the quality of traditional Chinese medicinal materials.