12 results about "Liquiritin" patented technology

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

The invention discloses an establishment method and an application method of a fingerprint spectrum of a honeysuckle and fructus forsythiae mixture, and belongs to the technical field of detection of effective components of traditional Chinese medicine, and the establishment method comprises the following steps: S1, determining chromatographic conditions; s2, preparing a test solution and a mixed reference solution; s3, collecting chromatograms of the test sample and the mixed reference substance; and S4, matching common peaks, calculating the relative retention time and the relative peak area of each common peak, comparing with the chromatogram of the reference substance, identifying the common peaks, and establishing the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture. According to the establishment method of the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture, reference and basis are provided for quality control of the honeysuckle and fructus forsythiae mixture, and the establishment method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like. The fingerprint spectrum has 11 effectively separated common peaks, identifies the characteristic peaks of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin and forsythiaside A, can reflect the condition of the effective components of the honeysuckle and fructus forsythiae mixture, and effectively characterizes the quality of the honeysuckle and fructus forsythiae mixture.

The invention discloses a method for simultaneously determining the contents of five components in a medicinal and edible beverage and application thereof. The method for simultaneously determining the contents of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage prepared from mulberry leaves, lotus leaves, mustard seeds and licorice roots under the same chromatographic condition by adopting a high performance liquid chromatography comprises the following steps: firstly preparing a water extract, a mixed reference solution and a test solution, and then detecting under a specific chromatographic condition to realize determination, so that the content of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage is determined. The method is simple and easy to operate, strong in specificity, repeatable, good in precision and stability, and capable of comprehensively reflecting the quality of the product. The method can also be used for standardized extraction and preparation of the medicinal and edible beverage. Through simultaneous determination of multiple indexes, an important method guarantee is provided for effective extraction and quality control of the medicinal and edible beverage with lower cost, higher popularity and more flexible operation space, the blank of quality control of multiple components in the medicinal and edible beverage is made up, and a preferable extraction process can be applied to production and has a wide application prospect. The practical application and popularization value is realized.

The invention belongs to the technical field of traditional Chinese medicine preparation component detection, and particularly relates to a method for detecting the content of main active components in ginger and liquorice decoction. According to the content detection method disclosed by the invention, flavonoid, triterpenoid saponin and phenol components in the ginger and liquorice decoction are detected by adopting an HPLC (High Performance Liquid Chromatography). Through specific sample pretreatment and chromatographic conditions, main active ingredients such as liquiritin, apiose isoliquiritin, isoliquiritin, liquiritigenin, mono-ammonium glycyrrhizinate and 6-gingerol in the main medicines ginger and liquorice in the ginger and liquorice decoction can be quantitatively detected at the same time, so that the clinical efficacy of the ginger and liquorice decoction preparation is guaranteed; and a more comprehensive method is provided for quality control of the ginger and licorice root soup.

The invention provides a construction method and application of a fingerprint spectrum of kidney-tonifying, blood-enriching and fetus-consolidating pills, and belongs to the technical field of medicines. The method comprises the following steps: extracting the kidney-tonifying, blood-enriching and fetus-consolidating pill by adopting a methanol aqueous solution, and taking the kidney-tonifying, blood-enriching and fetus-consolidating pill extracting solution as a test solution; taking a methanol solution of paeoniflorin, ferulic acid, liquiritin, baicalin, quercetin, baicalein and schizandrin as a reference substance solution; respectively carrying out high performance liquid chromatography analysis on the reference substance solution and the test solution, and taking the chromatographic peak of the reference substance solution as the reference to obtain the fingerprint spectrum of the kidney-tonifying, blood-enriching and fetus-consolidating pill. The fingerprint spectrum of the kidney-tonifying, blood-enriching and fetus-consolidating pills is constructed by adopting the high performance liquid chromatography, the quality of the kidney-tonifying, blood-enriching and fetus-consolidating pills can be comprehensively reflected, and the method can be used for quality control of the kidney-tonifying, blood-enriching and fetus-consolidating

The invention discloses a fingerprint spectrum of pills for strengthening body resistance and treating stranguria as well as a construction method and application thereof, and belongs to the technical field of quality detection of traditional Chinese medicine preparations. The method comprises the following steps: respectively injecting a test solution, a reference solution and a negative test solution into an ultra-high performance liquid chromatograph for determination by adopting ultra-high performance liquid chromatography, recording chromatograms, establishing a fingerprint spectrum of the pills for strengthening the body resistance and treating stranguria under the same chromatographic condition, calibrating 17 characteristic peaks, and determining the quality of the pills for strengthening the body resistance and treating stranguria. The five components of calycosin-7-glucoside, liquiritin, bisdemethoxycurcumin, demethoxycurcumin and curcumin in the pills for strengthening the body resistance and treating stranguria are quantitatively analyzed, so that content determination and quality detection are realized. The fingerprint spectrum of the pills for strengthening the body resistance and treating stranguria established on the basis of the ultra-high performance liquid chromatography and the multi-index component content determination method are simple, convenient and efficient, and the quality of the pills for strengthening the body resistance and treating stranguria can be comprehensively, systematically and objectively evaluated.

A quality control method for a Ramulus cinnamomi decoction with Pueraria lobata, which method is applied to quality control over the Ramulus cinnamomi decoction with Pueraria lobata by using puerarin, paeoniflorin and liquiritin as detection markers. The quality control method provided by the present invention can more effectively and comprehensively detect and monitor the quality of the Ramulus cinnamomi decoction with Pueraria lobata, and can better distinguish Ramulus cinnamomi decoctions with Pueraria lobata having different qualities, thereby avoiding the quality of the Ramulus cinnamomi decoctions with Pueraria lobata being uneven. In addition, the quality control method provides a scientific quality control and evaluation system for standardized production in the pharmaceutical industry.

The invention discloses a Ganhai Weikang capsule specific chromatogram based on a multi-component material basis as well as a construction method and application thereof, and belongs to the technical field of quality control and evaluation of Chinese patent medicines. A high performance liquid chromatography-high resolution mass spectrometry method is adopted for detection, and glycyrrhizic acid, liquiritin, liquiritigenin, apiose liquiritin, naringenin, chlorogenic acid, syringic acid, protocatechuic acid, hesperidin, narirutin, p-coumaric acid and kaempferide are taken as reference substances; the specific chromatogram is successfully constructed by detecting different batches of Ganhai Weikang capsules. The method for detecting the specific chromatogram of the Ganhai Weikang capsule, established by the invention, is simple and convenient to operate, high in precision and rapid in analysis, can realize efficient and synchronous detection of 12 characteristic components, and has good stability, accuracy and reproducibility. According to the method, synchronous and rapid analysis of multiple components is realized, the detection efficiency is remarkably improved, and reliable scientific basis and technical support are provided for quality control and clinical curative effect guarantee of the Ganhai Weikang capsules.

The invention discloses a method for simultaneously determining multiple indexes of a Mongolian medicine, i.e., Noutler-Wudahler, belongs to the technical field of medicine analysis, and solves the technical problem that various effective components and heavy metal elements in the Mongolian medicine cannot be rapidly and accurately determined at the same time by a traditional method. According to the technical scheme, the method is characterized by comprising the following steps: simultaneously determining the contents of eucalyptol, linalool, eugenol, alantolactone, isoalantolactone and dehydrocostunolide in a sample by adopting a gas chromatography-mass spectrometry method, wherein mass spectrometry detection adopts a multi-reaction monitoring mode and optimizes monitoring ion pairs and collision energy; simultaneously determining the contents of liquiritin, glycyrrhizic acid, gallic acid, piperine and alantolactone in the sample by adopting a liquid chromatography-mass spectrometry method; and simultaneously determining the contents of lead, cadmium, arsenic, mercury and copper heavy metal elements in the sample by adopting inductively coupled plasma mass spectrometry. The method is mainly used for controlling the quality of the Mongolian medicine, namely the frontalon-Wusunhler, so that the safety and effectiveness of the medicine are ensured.

The application discloses a quality control method of Houpu Qiwu Decoction, which comprises the following steps: S1, preparation of a test sample solution; S2, preparation of a control sample solution; S3, determination: respectively sucking the control sample solution and the test sample solution into a liquid chromatograph to perform high performance liquid chromatography analysis, recording chromatographic peaks, and obtaining a fingerprint spectrum of the Houpu Qiwu Decoction; high performance liquid chromatography conditions comprise using octadecylsilane bonded silica gel as a filler, using acetonitrile as a mobile phase A, and using a 0.1% phosphoric acid solution by volume fraction as a mobile phase B to perform gradient elution, and the fingerprint spectrum is determined by using double wavelengths: the detection wavelength is 320 nm at 0-32 min, and the detection wavelength is 254 nm at 32-70 min. The fingerprint spectrum of a Houpu Qiwu Decoction reference sample is established by using an ultra-high performance liquid chromatography method, and the contents of 12 kinds of index components such as glycyrrhizin are determined, so as to reflect the quality characteristics of the Houpu Qiwu Decoction reference sample.

The present application relates to a method for detecting the contents of paeoniflorin, glycyrrhizin, glycyrrhizic acid, paeonol and ginsenoside in a sample and application in quality control of Wenjing Decoction. The detection method comprises: performing first extraction treatment on the sample, collecting the first extraction liquid for first liquid chromatography detection, and determining the contents of paeoniflorin, glycyrrhizin, glycyrrhizic acid and / or paeonol in the sample based on the detection result; and / or performing second extraction treatment on the sample, collecting the second extraction liquid for second liquid chromatography detection, and determining the content of ginsenoside in the sample based on the detection result. The detection method of the present application can accurately detect the contents of paeoniflorin, glycyrrhizin, glycyrrhizic acid, paeonol and / or ginsenoside contained in the sample, has high accuracy, good precision, high sensitivity, good reproducibility, simple operation, short time, etc. The quality control method of Wenjing Decoction of the present application has the advantages of stability, controllability, good reproducibility, strong operability, etc., and the quality transfer traceability in the decoction process is strong.

The invention relates to the technical field of biological medicine, and provides application of liquiritin in preparation of a medicine for preventing / treating allergic asthma aiming at the problem that the existing medicine for treating allergic asthma can generate large side effects after being used for a long time. It is verified that liquiritin can improve balance through regulation and control of a channel, plays an anti-inflammatory role, has the effect of remarkably inhibiting inflammatory factors of allergic asthma and has a great industry prospect in preparation of the medicine for preventing / treating allergic asthma.