48 results about "Liquiritin" patented technology

The invention discloses a determination method of a UPLC fingerprint spectrum of children heat-clearing and cough-relieving granules, and belongs to the technical field of pharmaceutical analysis. According to the invention, the children's heat-clearing and cough-relieving granules are subjected to UPLC chromatographic analysis, and a UPLC fingerprint spectrum containing 16 common characteristic peaks including ephedrine hydrochloride, pseudoephedrine hydrochloride, amygdalin, liquiritin, baicalin and baicalein is established, so that the overall quality of the children's heat-clearing and cough-relieving granules can be evaluated more comprehensively; a new technical thought is provided for quality control and quality evaluation of the heat-clearing and cough-relieving granules for children.

The invention discloses a substance-based quality detection method for Xiaohuang powder, and belongs to the technical field of quality detection of traditional Chinese medicines. According to the method, standard control of a classic famous prescription is realized by constructing a multi-dimensional quality control system. The method comprises the following steps: firstly, carrying out specific identification on gardenia, divaricate saposhnikovia root, liquorice and pogostemon cablin in the prescription by adopting a thin-layer chromatography; meanwhile, a multi-index component content determination method is established, and the contents of five components including geniposide, cimicifugin, 5-O-methylvisammioside, liquiritin and glycyrrhizic acid are synchronously determined by applying a high performance liquid chromatography (HPLC). Through a system quality control strategy combining qualitative identification and quantitative analysis, not only is a quality standard system of the substance reference of the Xiaohuang powder perfected, but also reliable technical guarantee is provided for ensuring the stability and consistency of product quality. The establishment of the method establishes a standardized method system for the substance-based quality control of the classical famous prescription Xiaohuang powder.

The invention provides a method for detecting the content of liquiritin and glycyrrhizic acid in liquorice decoction pieces based on hyperspectrum, and relates to a method for rapidly and nondestructively predicting the content of index components of the liquorice decoction pieces by using a hyperspectrum technology. The method comprises the following implementation steps: acquiring a hyperspectral image of the liquorice decoction piece, determining the liquid phase content of the liquorice decoction piece, preprocessing the hyperspectral image, establishing a content prediction model, and quickly and nondestructively detecting the liquorice decoction piece. The method comprises the following steps: collecting licorice decoction pieces in a short-wave infrared band by using a hyperspectral imaging technology, determining the contents of liquiritin and glycyrrhizic acid in a collected sample through liquid chromatography, extracting characteristic wavelengths according to the content measurement data, comparing the advantages and disadvantages of different characteristic variable extraction methods and content prediction models, and determining the content of glycyrrhizic acid in the licorice decoction pieces. Therefore, rapid nondestructive detection of the liquiritin and glycyrrhizic acid content in the licorice decoction pieces is realized, an analysis method is provided for rapid grading and screening of the traditional Chinese medicine decoction pieces, digitization, intelligence and greenization of the traditional Chinese medicine industry are promoted, and improvement of the equipment manufacturing level of the pharmaceutical industry is promoted.

The invention discloses a dysphagia pill fingerprint spectrum establishment method and an established fingerprint spectrum, belongs to the technical field of analysis and detection of traditional Chinese medicine preparations, and provides a dysphagia pill fingerprint spectrum establishment method which comprises the following steps: mixing a dysphagia pill test sample with petroleum ether, performing Soxhlet extraction, mixing medicine residues with a methanol aqueous solution, performing heating reflux, performing column chromatography, and performing freeze drying to obtain a dysphagia pill fingerprint spectrum. Taking filtrate to obtain a test solution; mixing ellagic acid, liquiritin, ammonium glycyrrhizinate and sesamin reference substances with methanol to obtain a mixed reference substance solution; sucking the test solution and the reference solution, injecting into a liquid chromatograph, and collecting a chromatogram; introducing the chromatogram of the test sample into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system, selecting common chromatographic peaks as common peaks, generating a dysphagia pill contrast fingerprint spectrum, and calculating the relative retention time and the relative peak area of each common peak to obtain the HPLC fingerprint spectrum of the dysphagia pill. The quality of the dysphagia pill can be comprehensively evaluated by using the map, and the quality stability, consistency and controllability of the dysphagia pill are ensured.

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

The invention discloses a tissue regeneration promoting jade red gel and a quality detection method thereof.The tissue regeneration promoting jade red gel is characterized in that on the basis of a single-factor test, the dosage of carbomer 940, medicine and triethanolamine is used as influence factors, and the optimal proportion of all components of the gel is determined through an orthogonal test method: the dosage of carbomer 940 is 1%, the medicine loading capacity is 11%, and the dosage of triethanolamine is 1%; the results of accelerated stability and long-term stability tests show that the preparation has good stability in the investigation period. According to the method, the optimal thin-layer chromatography development condition is screened out, the radix angelicae sinensis, the radix arnebiae seu lithospermi and the sangusis draconis in the tissue regeneration promoting jade red gel are subjected to qualitative identification research, and chromatographic conditions such as the optimal flowability composition and a gradient elution mode are screened out through a large number of experiments; according to the present invention, with the high performance liquid chromatography method, the contents of the effective components such as ferulic acid, liquiritin, imperatorin, ligustilide, isoimperatorin and the like can be simultaneously determined, and the methodological detection results show that the high performance liquid chromatography method has characteristics of high precision, high accuracy, good repeatability and good stability.

The invention discloses a heart-protecting granule identification and quality control method based on thin layer chromatography, and belongs to the technical field of medicine detection. The thin-layer chromatography identification method comprises the following steps: identifying astragalus membranaceus by taking astragaloside as a reference substance, identifying wine-treated rhubarb by taking chrysophanol and emodin as reference substances, identifying leech by taking a leech medicinal material as a reference substance, identifying liquorice by taking liquiritin and a liquorice medicinal material as reference substances, and identifying radix rehmanniae by taking catalpol as a reference substance. And identifying salvia miltiorrhiza by taking salvianolic acid B and salvia miltiorrhiza medicinal materials as reference substances, and identifying fructus aurantii by taking naringin and neohesperidin as reference substances. By establishing the thin-layer chromatography identification method of the heart-protecting granules, an exclusive developing solvent and a developing mode are adopted, accurate identification is achieved according to the characteristics of spot colors, fluorescence characteristics and the like of corresponding positions of a test substance chromatography, a reference substance and a reference medicinal material chromatography, and the method is easy and convenient to operate, low in detection cost and high in repeatability and specificity; and quality control can be effectively carried out in the production process of the heart-protecting granules.

The present invention discloses a method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills, and belongs to the technical field of chemical detection. The method for simultaneously detecting seven effective ingredients in Yangyin Qingfei Pills described in the present invention comprises the following steps: injecting the Yangyin Qingfei Pill sample solution into a liquid chromatograph, and using a segmented wavelength conversion method to perform chromatographic detection to achieve qualitative and quantitative detection of the seven effective ingredients. The present invention uses the UPLC segmented wavelength conversion method to achieve simultaneous determination of the contents of harpagine, paeoniflorin, liquiritin, verbascoside, harpagine, paeonol and glycyrrhizic acid in Yangyin Qingfei Pills, and all seven effective ingredients can be well separated without interference from other ingredients. Moreover, each component has a good linear relationship within its own linear range, and the sample recovery rate is between 95% and 105%. The detection method of the present invention is simple, accurate and effective, and can be used for quality control and overall quality evaluation of Yangyin Qingfei Pills.

The invention belongs to the technical field of medicine detection, and particularly relates to a quality evaluation method for phlegm and fluid retention pills based on HPLC fingerprint spectrum and multi-index component content determination. The method comprises the following steps: determining the contents of nine active components in the phlegm-drinking pill and constructing an HPLC fingerprint spectrum of the phlegm-drinking pill based on a high performance liquid chromatography-high resolution mass spectrometry technology; wherein the nine active ingredients are sinapine, chlorogenic acid, cryptochlorogenic acid, 3, 6 '-dimustard acyl sucrose, rosmarinic acid, apiose isoliquiritin, isoliquiritin, glycyrrhizic acid and atractylenolide I. The invention further discloses a preparation method of the pharmaceutical composition. According to the method, the fingerprint spectrum and multi-index determination are integrated, a reproducible technical framework is provided for modern quality evaluation of the traditional Chinese medicine compound preparation with lower instrument cost and higher universality, and the industry is promoted to be transformed from'single component control 'to'overall quality management and control'.

The invention discloses an establishment method and an application method of a fingerprint spectrum of a honeysuckle and fructus forsythiae mixture, and belongs to the technical field of detection of effective components of traditional Chinese medicine, and the establishment method comprises the following steps: S1, determining chromatographic conditions; s2, preparing a test solution and a mixed reference solution; s3, collecting chromatograms of the test sample and the mixed reference substance; and S4, matching common peaks, calculating the relative retention time and the relative peak area of each common peak, comparing with the chromatogram of the reference substance, identifying the common peaks, and establishing the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture. According to the establishment method of the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture, reference and basis are provided for quality control of the honeysuckle and fructus forsythiae mixture, and the establishment method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like. The fingerprint spectrum has 11 effectively separated common peaks, identifies the characteristic peaks of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin and forsythiaside A, can reflect the condition of the effective components of the honeysuckle and fructus forsythiae mixture, and effectively characterizes the quality of the honeysuckle and fructus forsythiae mixture.

The present invention relates to a method for establishing a fingerprint spectrum of a Kemin capsule. By examining different sample solution preparations and chromatographic conditions, the present invention determines the sample solution preparation method and detection conditions for the product's fingerprint spectrum, identifies four characteristic peaks, namely, liquiritin, benzoylmonaconitine, ammonium glycyrrhizate, and ephedra hydrochloride, and effectively reflects the material basis of the Kemin capsule. This method can serve as a basis for quality control of the Kemin capsule and is used to evaluate the authenticity, excellence, and stability of the preparation.

The invention discloses a construction method of a trichosanthes kirilowii maxim and burdock soup fingerprint spectrum, and relates to the technical field of analytical chemistry. The determination method disclosed by the invention is simple and has the characteristics of high precision, stability and repeatability, and through determination, seven components including geniposide, liquiritin, hesperidin, arctiin, baicalin, oroxylin A-7-O-beta-D-glucuronide and wogonoside can be used as common characteristic peaks of the fingerprint spectrum of the trichosanthes kirilowii Maxim and arctium lappa decoction for identification and research.

The invention belongs to the field of analysis of traditional Chinese medicine preparations, and particularly relates to a method for determining the contents of seven active ingredients in Xiangsha Liujunzi decoction of an ancient classical famous prescription. According to the method, the contents of liquiritin, apiose liquiritin, narirutin, hesperidin, nobiletin, costunolide and dehydrocostus lactone in the Xiangsha Liujunzi decoction are simultaneously separated and measured under the same experimental condition by utilizing a high performance liquid chromatography (HPLC). Experimental results show that the method for simultaneously determining the contents of a plurality of different index components in the preparation through HPLC is simple, rapid, accurate, reliable and good in repeatability, stability and the like, and can be used as a quality control method of the preparation.

The invention provides a quality detection method of bulbus fritillariae cirrhosae lung-heat-clearing syrup. According to the quality detection method, the contents of amygdalin, sipeimine, liquiritin and platycodin in the bulbus fritillariae cirrhosae lung-heat-clearing syrup are detected by adopting an HPLC-ELSD (High Performance Liquid Chromatography-Evaporative Light Scattering Detector) chromatography. According to the method disclosed by the invention, the contents of four components, namely amygdalin, sipeimine, liquiritin and platycodin D, in the bulbus fritillariae cirrhosae lung-heat-clearing syrup are determined by adopting an HPLC-ELSD method, so that the quality of the bulbus fritillariae cirrhosae lung-heat-clearing syrup can be relatively rapidly evaluated, and reference is expected to be provided for quality control of a preparation. According to the method, interference of strong ultraviolet absorption substances is effectively avoided, the research cost is reduced, the operation process is simplified, an economic and efficient analysis means is provided for determining the content of saponin compounds, and meanwhile, a reference is provided for formulating a quality control standard of the bulbus fritillariae cirrhosae lung-heat-clearing syrup.

The invention discloses a construction method of an eight-treasure paste fingerprint spectrum, the fingerprint spectrum and application. The construction method comprises the following steps: weighing eight-treasure paste, and adding the weighed eight-treasure paste into a methanol solution to obtain a test sample; 5-hydroxymethylfurfural, paeoniflorin, chlorogenic acid, baicalin, ammonium glycyrrhizinate and liquiritin are weighed and added into a methanol solution, and a reference substance is obtained; respectively carrying out high performance liquid chromatography on the test sample and the reference substances to respectively obtain chromatograms of the test sample and the reference substances; determining a common peak according to the chromatogram of the test sample, comparing the common peak with the chromatogram of the reference substance, identifying the chemical components of the common peak, then selecting a reference peak, calculating the relative retention time of the common peak, and constructing to obtain the eight-treasure cream fingerprint spectrum. The construction method of the Bazhen cream fingerprint spectrum is beneficial to obtaining of more chromatographic peaks so as to realize overall detection of multiple components of the Bazhen cream, and the number of the chromatographic peaks of a liquid chromatogram obtained under the condition of gradient elution is large and the degree of separation is good.

The invention relates to a fingerprint spectrum of traditional Chinese medicine granules for treating nasosinusitis as well as a construction method and a detection method thereof, and belongs to the technical field of traditional Chinese medicine analysis. Comprising the following steps: step 1, preparing a test solution; 2, preparing a mixed reference substance solution; and step 3, performing high performance liquid chromatography detection. The invention further discloses a fingerprint spectrum and a detection method of the traditional Chinese medicine granules for treating nasosinusitis. According to the method disclosed by the invention, the characteristic chromatogram of the traditional Chinese medicine granules for treating nasosinusitis, which is good in separation degree, large in information amount, more in peak identification information and stable in base line, can be obtained, seven common characteristic peaks are determined, and the characteristic peaks of ferulic acid, chlorogenic acid, taxifolin, baicalin, liquiritin, ammonium glycyrrhizinate and quercitrin are successfully identified; the oneness and one-sidedness of quality control are avoided, and the method can be used for quality control and evaluation of the traditional Chinese medicine granules for treating nasosinusitis.

The present invention provides a high performance liquid chromatography (HPLC) detection method for index components in a Qinzhu Liangxue mixture. The HPLC method is used to detect nine components in the traditional Chinese medicine composition, namely, liquiritin, cimicifugin, 5-O-methylvisamin glycoside, liquiritigenin, baicalin, ethyl p-hydroxybenzoate, paeonol, baicalein and wogonin, so as to more comprehensively characterize the quality of the traditional Chinese medicine composition.

The invention discloses a method for simultaneously determining the contents of five components in a medicinal and edible beverage and application thereof. The method for simultaneously determining the contents of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage prepared from mulberry leaves, lotus leaves, mustard seeds and licorice roots under the same chromatographic condition by adopting a high performance liquid chromatography comprises the following steps: firstly preparing a water extract, a mixed reference solution and a test solution, and then detecting under a specific chromatographic condition to realize determination, so that the content of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage is determined. The method is simple and easy to operate, strong in specificity, repeatable, good in precision and stability, and capable of comprehensively reflecting the quality of the product. The method can also be used for standardized extraction and preparation of the medicinal and edible beverage. Through simultaneous determination of multiple indexes, an important method guarantee is provided for effective extraction and quality control of the medicinal and edible beverage with lower cost, higher popularity and more flexible operation space, the blank of quality control of multiple components in the medicinal and edible beverage is made up, and a preferable extraction process can be applied to production and has a wide application prospect. The practical application and popularization value is realized.

The present invention belongs to the field of analysis of traditional Chinese medicine preparations, and specifically relates to a method for determining the contents of seven active ingredients in the classic ancient prescription Xiangsha Liujunzi Decoction. This method utilizes high-performance liquid chromatography (HPLC) under the same experimental conditions to simultaneously separate and determine the contents of liquiritin, apigenin, naringin, hesperidin, nobiletin, costunolide, and dehydrocostuslactone in Xiangsha Liujunzi Decoction. Experimental results demonstrate that the HPLC method established herein for simultaneously determining the contents of multiple different index components in a preparation is simple, rapid, accurate, reliable, and exhibits good repeatability and stability, making it suitable for use as a quality control method for the preparation.

The invention discloses a miRNA marker for diagnosing and treating colorectal cancer and application of the miRNA marker in preparation of medicines for preventing and treating colorectal cancer, and belongs to the technical field of biological medicines. The miRNA is selected from one or more of the following components: hsa-miR-4488, hsa-miR-4525, hsa-miR-3130-3p, hsa-miR-6805-5p, hsa-miR-365b-3p, hsa-miR-3529-3p, hsa-miR-1255b-5p, hsa-miR-23b-3p, hsa-miR-652-5p, and hsa-miR-514b-5p, and the miRNA is selected from one or more of the following components: a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, a, the invention also provides application of the miRNA as a colorectal cancer detection or treatment target. The novel miRNA has the beneficial effects that the novel miRNA can be used as a colorectal cancer detection or treatment target, and whether a subject suffers from the colorectal cancer or is at the risk of colorectal cancer can be diagnosed by measuring the expression level of the miRNA. The diagnosis efficiency of the colorectal cancer is improved, and a new treatment target is provided for treating the colorectal cancer. The invention provides the application of liquiritin in preparation of medicines for preventing and treating colorectal cancer by regulating the expression quantity of miRNA or miR-23b, provides a new medical application of liquiritin, has better application prospect and medical value, and provides a new research direction for treatment of colorectal cancer.

The present invention provides a method for determining the fingerprint and multi-component content of Xiao Chaihu Granules. Using optimized pretreatment and high-performance liquid chromatography, the method enables qualitative analysis of 18 components from three medicinal herbs in Xiao Chaihu Granules using the same chromatographic conditions. Simultaneously, it quantitatively analyzes seven chemical components in Xiao Chaihu Granules: liquiritin, baicalin, wogonin, baicalein, glycyrrhizic acid, saikosaponin B2, and saikosaponin B1. This method addresses the technical issues of Xiao Chaihu Granules' complex chemical composition, the mutual interference of component chromatographic peaks, and the difficulty in comprehensively controlling drug quality. This method allows for more effective quality control of Xiao Chaihu Granules.

The invention belongs to the technical field of traditional Chinese medicine preparation component detection, and particularly relates to a method for detecting the content of main active components in ginger and liquorice decoction. According to the content detection method disclosed by the invention, flavonoid, triterpenoid saponin and phenol components in the ginger and liquorice decoction are detected by adopting an HPLC (High Performance Liquid Chromatography). Through specific sample pretreatment and chromatographic conditions, main active ingredients such as liquiritin, apiose isoliquiritin, isoliquiritin, liquiritigenin, mono-ammonium glycyrrhizinate and 6-gingerol in the main medicines ginger and liquorice in the ginger and liquorice decoction can be quantitatively detected at the same time, so that the clinical efficacy of the ginger and liquorice decoction preparation is guaranteed; and a more comprehensive method is provided for quality control of the ginger and licorice root soup.

The invention discloses an establishment method and application of a fingerprint spectrum of a Sanyone-fish-dragon mixture. The establishment method mainly comprises the steps of preparation of a test solution, preparation of a mixed reference substance solution, high performance liquid chromatography detection, establishment of a standard fingerprint spectrum and the like. According to the invention, the standard fingerprint spectrum of the Sapocorrhiza mixture is established, and the method is high in sensitivity, strong in specificity, easy to operate and accurate in result. The standard fingerprint spectrum constructed by the method has 36 characteristic peaks, 8 chemical components such as hesperidin, naringin, ammonium glycyrrhizinate, liquiritin, amygdalin, ferulic acid, hyperoside, quercetin and the like are effectively identified, the problem that the evaluation index of the Sanyone mixture is single is solved, the overall quality condition can be efficiently and comprehensively reflected, and the method has good application prospects. And a basis is provided for quality control and identification of the Sapococalyx mixture.

The invention provides a construction method and application of a fingerprint spectrum of kidney-tonifying, blood-enriching and fetus-consolidating pills, and belongs to the technical field of medicines. The method comprises the following steps: extracting the kidney-tonifying, blood-enriching and fetus-consolidating pill by adopting a methanol aqueous solution, and taking the kidney-tonifying, blood-enriching and fetus-consolidating pill extracting solution as a test solution; taking a methanol solution of paeoniflorin, ferulic acid, liquiritin, baicalin, quercetin, baicalein and schizandrin as a reference substance solution; respectively carrying out high performance liquid chromatography analysis on the reference substance solution and the test solution, and taking the chromatographic peak of the reference substance solution as the reference to obtain the fingerprint spectrum of the kidney-tonifying, blood-enriching and fetus-consolidating pill. The fingerprint spectrum of the kidney-tonifying, blood-enriching and fetus-consolidating pills is constructed by adopting the high performance liquid chromatography, the quality of the kidney-tonifying, blood-enriching and fetus-consolidating pills can be comprehensively reflected, and the method can be used for quality control of the kidney-tonifying, blood-enriching and fetus-consolidating

The invention provides an establishment method and application of a fingerprint spectrum of a children cough and asthma oral liquid, and belongs to the technical field of traditional Chinese medicine, the establishment method comprises the following steps: S1, dissolving the children cough and asthma oral liquid in an ethanol aqueous solution, extracting, filtering, taking filtrate, drying to obtain an extract, and dissolving the extract in a methanol aqueous solution to obtain a test solution; s2, respectively taking chlorogenic acid reference substances, cryptochlorogenic acid reference substances, liquiritin reference substances and ammonium glycyrrhizinate reference substances to prepare reference substance solutions; and S3, respectively and precisely sucking the reference solution and the test solution, performing sample introduction, performing determination by using a high performance liquid chromatograph, and establishing the fingerprint spectrum of the infantile cough and asthma oral liquid. The method is good in separation degree, accurate, reliable, simple to operate, high in precision, good in specificity, stability and repeatability, the established fingerprint spectrum of the children cough and asthma oral liquid is good in stability, and the quality of the children cough and asthma oral liquid can be more perfectly and scientifically controlled.

The present invention provides an analytical detection method for Ling Gui Zhu Gan Decoction. The method uses ultra-high performance liquid chromatography (UPLC) for detection and can be used for fingerprint detection of 14 components in Ling Gui Zhu Gan Decoction, including apiosyl liquiritin, liquiritin, apiosyl isoliquiritin, isoliquiritin, glycyrrhizin, cinnamic acid, cinnamaldehyde, o-methoxycinnamic acid, glycyrrhizic acid, atractylodes lactone III, atractylodes lactone II, pachymosin B, policocoric acid C and dehydropachymosic acid. The method can also be used for content control of liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizic acid. The method has strong applicability and is of great significance for comprehensive and objective evaluation of Ling Gui Zhu Gan Decoction.

The invention relates to the technical field of traditional Chinese medicine analysis, in particular to a construction method of a fingerprint spectrum of infantile seven-star tea granules and the fingerprint spectrum of the infantile seven-star tea granules. The construction method comprises the following steps: preparation of a test solution and preparation of a reference solution; respectively injecting the reference substance solution and the test substance solution into a high performance liquid chromatograph for determination to obtain a reference substance spectrum and a test substance spectrum; liquiritin is selected as a reference peak in the atlas of the test sample; importing the fingerprint spectrum of the test sample into a traditional Chinese medicine chromatographic fingerprint spectrum similarity evaluation system to generate a control fingerprint spectrum, and establishing a common mode to obtain 46 common peaks; and calculating the relative retention time and the relative peak area of each common peak according to the selected reference peak. The construction method disclosed by the invention is high in sensitivity, good in accuracy, strong in repeatability and rapid in analysis, 46 common peaks are determined for 15 batches of samples, and the similarity between the samples and a control fingerprint spectrum is high.

The invention provides a supercritical fluid chromatography technology-based traditional Chinese medicine compound multi-component extraction method, which comprises: (1) carrying out pretreatment on traditional Chinese medicine compound Xiaoyao powder to obtain a sample to be detected; (2) preparing standard solutions of apiose liquiritin, apiose isoliquiritin, paeoniflorin, albiflorin, saikoside A and saikoside D; and (3) carrying out supercritical fluid chromatographic analysis on the to-be-detected sample, and extracting. According to the extraction method of the traditional Chinese medicine compound multi-component based on the supercritical fluid chromatography technology, the simultaneous preparation of the multi-component (apiose liquiritin, apiose isoliquiritin, paeoniflorin, albiflorin, saikoside A and saikoside D) in the traditional Chinese medicine compound preparation Xiaoyao powder can be realized.

The invention discloses application of PTPRG as a marker and a therapeutic target for diagnosing colorectal cancer, and belongs to the technical field of biological medicines, reagents for detecting the expression level of PTPRG comprise a reagent for detecting the expression level of PTPRG gene mRNA and / or a reagent for detecting the expression level of PTPRG protein. The invention also provides application of PTPRG as a regulation target spot in preparation of medicines for treating colorectal cancer. The application has the beneficial effects that a new therapeutic target for colorectal cancer is provided, a new thought is provided for treatment of colorectal cancer, it is revealed that liquiritin inhibits miR-23b to promote expression of PTPRG in a targeted mode, then the effect of preventing and treating colorectal cancer is achieved, and the application prospect and economic value are wide.

The invention relates to the technical field of medicine quality control, in particular to a method for simultaneously determining multiple important components in a heart-nourishing and palpitation-calming capsule based on LC-MS / MS. The method can be used for synchronously determining the contents of five components such as liquiritigenin, liquiritin, rehmannia glutinosa liboside D, ginsenoside Re and ginsenoside Rg1 in the heart-nourishing and palpitation-calming capsule. In the detection process, extraction conditions, chromatographic conditions, ionization modes and the like are optimized, so that chromatographic peaks of LC-MS / MS in the detection method are good in linear relation (r is greater than or equal to 0.9992) in respective ranges, the average sample adding recovery rate is 98.91%-100.55%, and RSD is 1.37%-1.78%. The methodological investigation proves that the method disclosed by the invention is good in repeatability and stability, has the characteristics of accuracy, sensitivity, strong specificity and the like, and can be used for controlling the quality of the heart-nourishing and palpitation-calming capsules.