27 results about "Liquiritin" patented technology

The invention discloses a substance-based quality detection method for Xiaohuang powder, and belongs to the technical field of quality detection of traditional Chinese medicines. According to the method, standard control of a classic famous prescription is realized by constructing a multi-dimensional quality control system. The method comprises the following steps: firstly, carrying out specific identification on gardenia, divaricate saposhnikovia root, liquorice and pogostemon cablin in the prescription by adopting a thin-layer chromatography; meanwhile, a multi-index component content determination method is established, and the contents of five components including geniposide, cimicifugin, 5-O-methylvisammioside, liquiritin and glycyrrhizic acid are synchronously determined by applying a high performance liquid chromatography (HPLC). Through a system quality control strategy combining qualitative identification and quantitative analysis, not only is a quality standard system of the substance reference of the Xiaohuang powder perfected, but also reliable technical guarantee is provided for ensuring the stability and consistency of product quality. The establishment of the method establishes a standardized method system for the substance-based quality control of the classical famous prescription Xiaohuang powder.

The invention provides a method for detecting the content of liquiritin and glycyrrhizic acid in liquorice decoction pieces based on hyperspectrum, and relates to a method for rapidly and nondestructively predicting the content of index components of the liquorice decoction pieces by using a hyperspectrum technology. The method comprises the following implementation steps: acquiring a hyperspectral image of the liquorice decoction piece, determining the liquid phase content of the liquorice decoction piece, preprocessing the hyperspectral image, establishing a content prediction model, and quickly and nondestructively detecting the liquorice decoction piece. The method comprises the following steps: collecting licorice decoction pieces in a short-wave infrared band by using a hyperspectral imaging technology, determining the contents of liquiritin and glycyrrhizic acid in a collected sample through liquid chromatography, extracting characteristic wavelengths according to the content measurement data, comparing the advantages and disadvantages of different characteristic variable extraction methods and content prediction models, and determining the content of glycyrrhizic acid in the licorice decoction pieces. Therefore, rapid nondestructive detection of the liquiritin and glycyrrhizic acid content in the licorice decoction pieces is realized, an analysis method is provided for rapid grading and screening of the traditional Chinese medicine decoction pieces, digitization, intelligence and greenization of the traditional Chinese medicine industry are promoted, and improvement of the equipment manufacturing level of the pharmaceutical industry is promoted.

This invention discloses a method for determining the content of target components in Qingning Powder. Stir-fried Lepidium apetalum seeds, stir-fried Plantago asiatica seeds, and stir-fried Glycyrrhiza uralensis are dissolved in methanol solution, shaken well, filtered, and the filtrate is used to obtain a test solution for detection by high-performance liquid chromatography (HPLC). The three target components in the test solution are identified as: quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside (C...). 33 H 40 O 22 ), genipinic acid (C 16 H 22 O 10 ), glycyrrhizin (C 21 H 22 The content of O9); the detection method using high performance liquid chromatography includes the following steps; Step 1: Take appropriate amounts of reference standards for quercetin-3-O-β-D-glucose-7-O-β-D-gentiopicroside, genipin glycoside, verbascoside, and glycyrrhizin, respectively, and weigh them accurately. This method for determining the content of target components in Qingning Powder, through monomer analysis combined with specific reference standards, breaks through the mixed bottleneck of the whole formula fingerprint spectrum and quickly locates the responsible medicinal materials of abnormal batches.

The invention discloses a tissue regeneration promoting jade red gel and a quality detection method thereof.The tissue regeneration promoting jade red gel is characterized in that on the basis of a single-factor test, the dosage of carbomer 940, medicine and triethanolamine is used as influence factors, and the optimal proportion of all components of the gel is determined through an orthogonal test method: the dosage of carbomer 940 is 1%, the medicine loading capacity is 11%, and the dosage of triethanolamine is 1%; the results of accelerated stability and long-term stability tests show that the preparation has good stability in the investigation period. According to the method, the optimal thin-layer chromatography development condition is screened out, the radix angelicae sinensis, the radix arnebiae seu lithospermi and the sangusis draconis in the tissue regeneration promoting jade red gel are subjected to qualitative identification research, and chromatographic conditions such as the optimal flowability composition and a gradient elution mode are screened out through a large number of experiments; according to the present invention, with the high performance liquid chromatography method, the contents of the effective components such as ferulic acid, liquiritin, imperatorin, ligustilide, isoimperatorin and the like can be simultaneously determined, and the methodological detection results show that the high performance liquid chromatography method has characteristics of high precision, high accuracy, good repeatability and good stability.

The invention discloses a heart-protecting granule identification and quality control method based on thin layer chromatography, and belongs to the technical field of medicine detection. The thin-layer chromatography identification method comprises the following steps: identifying astragalus membranaceus by taking astragaloside as a reference substance, identifying wine-treated rhubarb by taking chrysophanol and emodin as reference substances, identifying leech by taking a leech medicinal material as a reference substance, identifying liquorice by taking liquiritin and a liquorice medicinal material as reference substances, and identifying radix rehmanniae by taking catalpol as a reference substance. And identifying salvia miltiorrhiza by taking salvianolic acid B and salvia miltiorrhiza medicinal materials as reference substances, and identifying fructus aurantii by taking naringin and neohesperidin as reference substances. By establishing the thin-layer chromatography identification method of the heart-protecting granules, an exclusive developing solvent and a developing mode are adopted, accurate identification is achieved according to the characteristics of spot colors, fluorescence characteristics and the like of corresponding positions of a test substance chromatography, a reference substance and a reference medicinal material chromatography, and the method is easy and convenient to operate, low in detection cost and high in repeatability and specificity; and quality control can be effectively carried out in the production process of the heart-protecting granules.

The invention belongs to the technical field of medicine detection, and particularly relates to a quality evaluation method for phlegm and fluid retention pills based on HPLC fingerprint spectrum and multi-index component content determination. The method comprises the following steps: determining the contents of nine active components in the phlegm-drinking pill and constructing an HPLC fingerprint spectrum of the phlegm-drinking pill based on a high performance liquid chromatography-high resolution mass spectrometry technology; wherein the nine active ingredients are sinapine, chlorogenic acid, cryptochlorogenic acid, 3, 6 '-dimustard acyl sucrose, rosmarinic acid, apiose isoliquiritin, isoliquiritin, glycyrrhizic acid and atractylenolide I. The invention further discloses a preparation method of the pharmaceutical composition. According to the method, the fingerprint spectrum and multi-index determination are integrated, a reproducible technical framework is provided for modern quality evaluation of the traditional Chinese medicine compound preparation with lower instrument cost and higher universality, and the industry is promoted to be transformed from'single component control 'to'overall quality management and control'.

The invention discloses an establishment method and an application method of a fingerprint spectrum of a honeysuckle and fructus forsythiae mixture, and belongs to the technical field of detection of effective components of traditional Chinese medicine, and the establishment method comprises the following steps: S1, determining chromatographic conditions; s2, preparing a test solution and a mixed reference solution; s3, collecting chromatograms of the test sample and the mixed reference substance; and S4, matching common peaks, calculating the relative retention time and the relative peak area of each common peak, comparing with the chromatogram of the reference substance, identifying the common peaks, and establishing the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture. According to the establishment method of the fingerprint spectrum of the honeysuckle and fructus forsythiae mixture, reference and basis are provided for quality control of the honeysuckle and fructus forsythiae mixture, and the establishment method has the advantages of being simple, convenient, stable, high in precision, good in reproducibility and the like. The fingerprint spectrum has 11 effectively separated common peaks, identifies the characteristic peaks of neochlorogenic acid, chlorogenic acid, cryptochlorogenic acid, liquiritin and forsythiaside A, can reflect the condition of the effective components of the honeysuckle and fructus forsythiae mixture, and effectively characterizes the quality of the honeysuckle and fructus forsythiae mixture.

The invention relates to a fingerprint spectrum of traditional Chinese medicine granules for treating nasosinusitis as well as a construction method and a detection method thereof, and belongs to the technical field of traditional Chinese medicine analysis. Comprising the following steps: step 1, preparing a test solution; 2, preparing a mixed reference substance solution; and step 3, performing high performance liquid chromatography detection. The invention further discloses a fingerprint spectrum and a detection method of the traditional Chinese medicine granules for treating nasosinusitis. According to the method disclosed by the invention, the characteristic chromatogram of the traditional Chinese medicine granules for treating nasosinusitis, which is good in separation degree, large in information amount, more in peak identification information and stable in base line, can be obtained, seven common characteristic peaks are determined, and the characteristic peaks of ferulic acid, chlorogenic acid, taxifolin, baicalin, liquiritin, ammonium glycyrrhizinate and quercitrin are successfully identified; the oneness and one-sidedness of quality control are avoided, and the method can be used for quality control and evaluation of the traditional Chinese medicine granules for treating nasosinusitis.

The invention discloses a method for simultaneously determining the contents of five components in a medicinal and edible beverage and application thereof. The method for simultaneously determining the contents of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage prepared from mulberry leaves, lotus leaves, mustard seeds and licorice roots under the same chromatographic condition by adopting a high performance liquid chromatography comprises the following steps: firstly preparing a water extract, a mixed reference solution and a test solution, and then detecting under a specific chromatographic condition to realize determination, so that the content of rutin, nuciferine, sinapine, liquiritin and glycyrrhizic acid in the medicinal and edible beverage is determined. The method is simple and easy to operate, strong in specificity, repeatable, good in precision and stability, and capable of comprehensively reflecting the quality of the product. The method can also be used for standardized extraction and preparation of the medicinal and edible beverage. Through simultaneous determination of multiple indexes, an important method guarantee is provided for effective extraction and quality control of the medicinal and edible beverage with lower cost, higher popularity and more flexible operation space, the blank of quality control of multiple components in the medicinal and edible beverage is made up, and a preferable extraction process can be applied to production and has a wide application prospect. The practical application and popularization value is realized.

The present invention belongs to the field of analysis of traditional Chinese medicine preparations, and specifically relates to a method for determining the contents of seven active ingredients in the classic ancient prescription Xiangsha Liujunzi Decoction. This method utilizes high-performance liquid chromatography (HPLC) under the same experimental conditions to simultaneously separate and determine the contents of liquiritin, apigenin, naringin, hesperidin, nobiletin, costunolide, and dehydrocostuslactone in Xiangsha Liujunzi Decoction. Experimental results demonstrate that the HPLC method established herein for simultaneously determining the contents of multiple different index components in a preparation is simple, rapid, accurate, reliable, and exhibits good repeatability and stability, making it suitable for use as a quality control method for the preparation.

The present invention provides a method for determining the fingerprint and multi-component content of Xiao Chaihu Granules. Using optimized pretreatment and high-performance liquid chromatography, the method enables qualitative analysis of 18 components from three medicinal herbs in Xiao Chaihu Granules using the same chromatographic conditions. Simultaneously, it quantitatively analyzes seven chemical components in Xiao Chaihu Granules: liquiritin, baicalin, wogonin, baicalein, glycyrrhizic acid, saikosaponin B2, and saikosaponin B1. This method addresses the technical issues of Xiao Chaihu Granules' complex chemical composition, the mutual interference of component chromatographic peaks, and the difficulty in comprehensively controlling drug quality. This method allows for more effective quality control of Xiao Chaihu Granules.

The invention belongs to the technical field of traditional Chinese medicine preparation component detection, and particularly relates to a method for detecting the content of main active components in ginger and liquorice decoction. According to the content detection method disclosed by the invention, flavonoid, triterpenoid saponin and phenol components in the ginger and liquorice decoction are detected by adopting an HPLC (High Performance Liquid Chromatography). Through specific sample pretreatment and chromatographic conditions, main active ingredients such as liquiritin, apiose isoliquiritin, isoliquiritin, liquiritigenin, mono-ammonium glycyrrhizinate and 6-gingerol in the main medicines ginger and liquorice in the ginger and liquorice decoction can be quantitatively detected at the same time, so that the clinical efficacy of the ginger and liquorice decoction preparation is guaranteed; and a more comprehensive method is provided for quality control of the ginger and licorice root soup.

The invention provides a construction method and application of a fingerprint spectrum of kidney-tonifying, blood-enriching and fetus-consolidating pills, and belongs to the technical field of medicines. The method comprises the following steps: extracting the kidney-tonifying, blood-enriching and fetus-consolidating pill by adopting a methanol aqueous solution, and taking the kidney-tonifying, blood-enriching and fetus-consolidating pill extracting solution as a test solution; taking a methanol solution of paeoniflorin, ferulic acid, liquiritin, baicalin, quercetin, baicalein and schizandrin as a reference substance solution; respectively carrying out high performance liquid chromatography analysis on the reference substance solution and the test solution, and taking the chromatographic peak of the reference substance solution as the reference to obtain the fingerprint spectrum of the kidney-tonifying, blood-enriching and fetus-consolidating pill. The fingerprint spectrum of the kidney-tonifying, blood-enriching and fetus-consolidating pills is constructed by adopting the high performance liquid chromatography, the quality of the kidney-tonifying, blood-enriching and fetus-consolidating pills can be comprehensively reflected, and the method can be used for quality control of the kidney-tonifying, blood-enriching and fetus-consolidating

The present invention provides an analytical detection method for Ling Gui Zhu Gan Decoction. The method uses ultra-high performance liquid chromatography (UPLC) for detection and can be used for fingerprint detection of 14 components in Ling Gui Zhu Gan Decoction, including apiosyl liquiritin, liquiritin, apiosyl isoliquiritin, isoliquiritin, glycyrrhizin, cinnamic acid, cinnamaldehyde, o-methoxycinnamic acid, glycyrrhizic acid, atractylodes lactone III, atractylodes lactone II, pachymosin B, policocoric acid C and dehydropachymosic acid. The method can also be used for content control of liquiritin, cinnamic acid, cinnamaldehyde and glycyrrhizic acid. The method has strong applicability and is of great significance for comprehensive and objective evaluation of Ling Gui Zhu Gan Decoction.

The invention relates to the technical field of component analysis, in particular to a method for determining the content of three chemical components in a liver-soothing and stomach-calming pill by adopting a high-efficiency capillary electrophoresis method, which comprises a content determination method for simultaneously determining one or more of liquiritin, naringin and neohesperidin. Different from an HPLC (High Performance Liquid Chromatography) method which is high in consumption cost, long in analysis time and large in organic solvent dosage, the method is simple and convenient, low in cost, rapid and accurate in determination result and good in repeatability, and a practical and simple method can be provided for quality control of the liver-soothing and stomach-calming pill.

The invention discloses a fingerprint spectrum of pills for strengthening body resistance and treating stranguria as well as a construction method and application thereof, and belongs to the technical field of quality detection of traditional Chinese medicine preparations. The method comprises the following steps: respectively injecting a test solution, a reference solution and a negative test solution into an ultra-high performance liquid chromatograph for determination by adopting ultra-high performance liquid chromatography, recording chromatograms, establishing a fingerprint spectrum of the pills for strengthening the body resistance and treating stranguria under the same chromatographic condition, calibrating 17 characteristic peaks, and determining the quality of the pills for strengthening the body resistance and treating stranguria. The five components of calycosin-7-glucoside, liquiritin, bisdemethoxycurcumin, demethoxycurcumin and curcumin in the pills for strengthening the body resistance and treating stranguria are quantitatively analyzed, so that content determination and quality detection are realized. The fingerprint spectrum of the pills for strengthening the body resistance and treating stranguria established on the basis of the ultra-high performance liquid chromatography and the multi-index component content determination method are simple, convenient and efficient, and the quality of the pills for strengthening the body resistance and treating stranguria can be comprehensively, systematically and objectively evaluated.

The present invention discloses a method for establishing a fingerprint spectrum of Yege Pills and the fingerprint spectrum obtained by establishing the fingerprint spectrum, belonging to the technical field of analysis and detection of traditional Chinese medicine preparations. The method for establishing a fingerprint spectrum of Yege Pills is provided, comprising: mixing a Yege Pill test sample with petroleum ether, performing Soxhlet extraction, mixing the drug residue with a methanol-water solution, heating and refluxing, and taking the filtrate to obtain a test solution; mixing ellagic acid, liquiritin, ammonium glycyrrhizate, and sesamin reference substances with methanol to obtain a mixed reference solution; aspirating the test solution and the reference solution, injecting them into a liquid chromatograph, and collecting a chromatogram; importing the test sample chromatogram into a "Chinese Medicine Chromatographic Fingerprint Similarity Evaluation System", selecting common chromatographic peaks as common peaks, generating a Yege Pill reference fingerprint spectrum, calculating the relative retention time and relative peak area of ​​each common peak, and obtaining an HPLC fingerprint spectrum of Yege Pills. The spectrum can be used to more comprehensively evaluate the quality of Yege Pills, ensuring the quality stability, consistency, and controllability of Yege Pills.

A quality control method for a Ramulus cinnamomi decoction with Pueraria lobata, which method is applied to quality control over the Ramulus cinnamomi decoction with Pueraria lobata by using puerarin, paeoniflorin and liquiritin as detection markers. The quality control method provided by the present invention can more effectively and comprehensively detect and monitor the quality of the Ramulus cinnamomi decoction with Pueraria lobata, and can better distinguish Ramulus cinnamomi decoctions with Pueraria lobata having different qualities, thereby avoiding the quality of the Ramulus cinnamomi decoctions with Pueraria lobata being uneven. In addition, the quality control method provides a scientific quality control and evaluation system for standardized production in the pharmaceutical industry.

The invention discloses a Ganhai Weikang capsule specific chromatogram based on a multi-component material basis as well as a construction method and application thereof, and belongs to the technical field of quality control and evaluation of Chinese patent medicines. A high performance liquid chromatography-high resolution mass spectrometry method is adopted for detection, and glycyrrhizic acid, liquiritin, liquiritigenin, apiose liquiritin, naringenin, chlorogenic acid, syringic acid, protocatechuic acid, hesperidin, narirutin, p-coumaric acid and kaempferide are taken as reference substances; the specific chromatogram is successfully constructed by detecting different batches of Ganhai Weikang capsules. The method for detecting the specific chromatogram of the Ganhai Weikang capsule, established by the invention, is simple and convenient to operate, high in precision and rapid in analysis, can realize efficient and synchronous detection of 12 characteristic components, and has good stability, accuracy and reproducibility. According to the method, synchronous and rapid analysis of multiple components is realized, the detection efficiency is remarkably improved, and reliable scientific basis and technical support are provided for quality control and clinical curative effect guarantee of the Ganhai Weikang capsules.

The invention discloses a method for simultaneously determining multiple indexes of a Mongolian medicine, i.e., Noutler-Wudahler, belongs to the technical field of medicine analysis, and solves the technical problem that various effective components and heavy metal elements in the Mongolian medicine cannot be rapidly and accurately determined at the same time by a traditional method. According to the technical scheme, the method is characterized by comprising the following steps: simultaneously determining the contents of eucalyptol, linalool, eugenol, alantolactone, isoalantolactone and dehydrocostunolide in a sample by adopting a gas chromatography-mass spectrometry method, wherein mass spectrometry detection adopts a multi-reaction monitoring mode and optimizes monitoring ion pairs and collision energy; simultaneously determining the contents of liquiritin, glycyrrhizic acid, gallic acid, piperine and alantolactone in the sample by adopting a liquid chromatography-mass spectrometry method; and simultaneously determining the contents of lead, cadmium, arsenic, mercury and copper heavy metal elements in the sample by adopting inductively coupled plasma mass spectrometry. The method is mainly used for controlling the quality of the Mongolian medicine, namely the frontalon-Wusunhler, so that the safety and effectiveness of the medicine are ensured.

The invention belongs to the technical field of traditional Chinese medicine preparation and quality control and detection of components thereof, and relates to a quality evaluation method of Chinese herbaceous peony and liquorice decoction. The invention provides the fingerprint spectrum of the Chinese herbaceous peony and liquorice decoction, and the variety, the transfer rule and the content of the internal chemical substances of the Chinese herbaceous peony and liquorice decoction are evaluated on the whole; the invention also provides a method for simultaneously detecting the contents of albiflorin, paeoniflorin, liquiritin and glycyrrhizic acid in the paeoniflorin and glycyrrhizic acid decoction and a rapid thin-layer chromatography identification method for white paeony root and glycyrrhizic acid in the paeoniflorin and glycyrrhizic acid decoction, which can objectively, comprehensively and accurately evaluate the quality of the paeoniflorin and glycyrrhizic acid decoction. Therefore, the purpose of more comprehensively and effectively controlling the product quality of the Chinese herbaceous peony and liquorice decoction composition preparation can be achieved, and the method has important significance for controlling the quality of Chinese herbaceous peony and liquorice decoction and ensuring the clinical curative effect.

The invention provides a fingerprint spectrum construction method of an ancient-Chinese health-preserving essence oral liquid and a multi-index component detection method, and relates to the technical field of analysis and detection. The fingerprint spectrum of the ancient-Chinese health-preserving essence oral liquid constructed by adopting a high-performance liquid chromatography has 20-50 common peaks, 9 chemical components are determined, ginsenoside Rb1 is a component in a monarch drug ginseng, calycosin-7-glucoside is a component in a monarch drug astragalus membranaceus, specnuezhenide is a component in a ministerial drug glossy privet fruit, and the fingerprint spectrum of the ancient-Chinese health-preserving essence oral liquid is a component in a ministerial drug glossy privet fruit. Epimedin A, Epimedin B, Epimedin C and icariin are all components in a ministerial drug of herba epimedii, paeoniflorin is a component in an adjuvant drug of radix paeoniae alba, and liquiritin is a component in a conductant drug of liquorice. According to the fingerprint spectrum of the ancient-Chinese health-preserving essence oral liquid, a plurality of substances in monarch, minister, assistant and guide are taken as quality indexes of the ancient-Chinese health-preserving essence oral liquid, so that accurate and comprehensive quality control on the quality of the ancient-Chinese health-preserving essence oral liquid can be realized, and too simple currently executed quality standards of the ancient-Chinese health-preserving essence oral liquid are perfected.

The invention discloses a method for constructing an HPLC (High Performance Liquid Chromatography) fingerprint spectrum of a spleen-tonifying, heat-clearing and stasis-dissipating drink and determining the content of multi-index components in the HPLC fingerprint spectrum, which comprises the following steps: detecting different batches of samples by taking 11 components such as tanshinol, liquiritin, hesperidin, lobetyolin, alkannic acid and the like as reference substances, adopting liquid chromatography and taking an acetonitrile-0. 3 vol% phosphoric acid aqueous solution as a mobile phase, and determining the content of the multi-index components in the fingerprint spectrum of the spleen-tonifying, heat-clearing and stasis-dissipating drink. And chemical component attribution and linear fitting are carried out on common peaks in the HPLC fingerprint spectrum, so that quantitative detection of the contents of the 11 components is realized. The method can realize qualitative and quantitative research on the spleen-tonifying, heat-clearing and blood-stasis-dissipating drink under the same chromatographic condition, and has a good application prospect in quality standard control of the spleen-tonifying, heat-clearing and blood-stasis-dissipating drink.

The invention discloses a Bazheng tablet HPLC (High Performance Liquid Chromatography) fingerprint spectrum as well as a construction method and application thereof, and belongs to the technical field of quality control of traditional Chinese medicine preparations. According to the method, high performance liquid chromatography and mass spectrometry are combined, and geniposidic acid, geniposide, liquiritin and the like are used as reference substances to accurately identify characteristic peaks in a fingerprint spectrum; by designing a gradient elution program, the resolution and accuracy of a chromatogram are improved; a plurality of batches of samples are systematically analyzed, the fingerprint spectrum is successfully constructed, the fingerprint spectrum realizes the characterization of 30 chromatographic common peaks, 10 main chromatographic peaks are clearly and chemically identified, a plurality of medicinal flavors such as liquorice and fructus gardeniae are covered, the chemical component information of the Bazheng tablets is comprehensively and accurately reflected, and the fingerprint spectrum has the advantages of reliability, high sensitivity, high accuracy and the like. Products of different batches and different manufacturers can be rapidly and accurately identified, the preparation quality is comprehensively evaluated, the product quality is ensured, and a powerful guarantee is provided for controlling the quality and ensuring the clinical curative effect.

The application discloses a quality control method of Houpu Qiwu Decoction, which comprises the following steps: S1, preparation of a test sample solution; S2, preparation of a control sample solution; S3, determination: respectively sucking the control sample solution and the test sample solution into a liquid chromatograph to perform high performance liquid chromatography analysis, recording chromatographic peaks, and obtaining a fingerprint spectrum of the Houpu Qiwu Decoction; high performance liquid chromatography conditions comprise using octadecylsilane bonded silica gel as a filler, using acetonitrile as a mobile phase A, and using a 0.1% phosphoric acid solution by volume fraction as a mobile phase B to perform gradient elution, and the fingerprint spectrum is determined by using double wavelengths: the detection wavelength is 320 nm at 0-32 min, and the detection wavelength is 254 nm at 32-70 min. The fingerprint spectrum of a Houpu Qiwu Decoction reference sample is established by using an ultra-high performance liquid chromatography method, and the contents of 12 kinds of index components such as glycyrrhizin are determined, so as to reflect the quality characteristics of the Houpu Qiwu Decoction reference sample.

The present application relates to a method for detecting the contents of paeoniflorin, glycyrrhizin, glycyrrhizic acid, paeonol and ginsenoside in a sample and application in quality control of Wenjing Decoction. The detection method comprises: performing first extraction treatment on the sample, collecting the first extraction liquid for first liquid chromatography detection, and determining the contents of paeoniflorin, glycyrrhizin, glycyrrhizic acid and / or paeonol in the sample based on the detection result; and / or performing second extraction treatment on the sample, collecting the second extraction liquid for second liquid chromatography detection, and determining the content of ginsenoside in the sample based on the detection result. The detection method of the present application can accurately detect the contents of paeoniflorin, glycyrrhizin, glycyrrhizic acid, paeonol and / or ginsenoside contained in the sample, has high accuracy, good precision, high sensitivity, good reproducibility, simple operation, short time, etc. The quality control method of Wenjing Decoction of the present application has the advantages of stability, controllability, good reproducibility, strong operability, etc., and the quality transfer traceability in the decoction process is strong.

The invention relates to the technical field of biological medicine, and provides application of liquiritin in preparation of a medicine for preventing / treating allergic asthma aiming at the problem that the existing medicine for treating allergic asthma can generate large side effects after being used for a long time. It is verified that liquiritin can improve balance through regulation and control of a channel, plays an anti-inflammatory role, has the effect of remarkably inhibiting inflammatory factors of allergic asthma and has a great industry prospect in preparation of the medicine for preventing / treating allergic asthma.