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Method for measuring seven functional components of liquorice in cosmetics

A technology for functional components and cosmetics, which is applied in the field of simultaneous determination of seven functional components of licorice root, can solve the problems that the determination of functional components of licorice root is still blank, cannot be analyzed and measured at the same time, and has no detection standards, and achieves a simple sample processing method, short time, and high efficiency. sharp peaks

Inactive Publication Date: 2014-05-28
LIAONING PROVINCE INST FOR FOOD & DRUG CONTROL
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  • Abstract
  • Description
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  • Application Information

AI Technical Summary

Problems solved by technology

Glycyrrhizae inspection standard, only one of the Chinese Pharmacopoeia 2010 edition, Glycyrrhiza glycosides and Glycyrrhizic acid are determined, and this method cannot simultaneously analyze and determine the above seven functional components of Glycyrrhiza licorice
In the field of cosmetics, so far, the determination of functional components of licorice is still blank, and no relevant testing standards have been issued. However, functional components of licorice are used more and more in cosmetics. All deserve our more and more attention

Method used

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  • Method for measuring seven functional components of liquorice in cosmetics
  • Method for measuring seven functional components of liquorice in cosmetics
  • Method for measuring seven functional components of liquorice in cosmetics

Examples

Experimental program
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Effect test

Embodiment 1

[0068]The sample to be processed is the facial mask in cosmetics (sample number is HC20120259): Squeeze the facial mask to get its liquid, accurately weigh two parallel samples of the liquid, put 0.8g (accurate to 0.01g) of each in a 15mL graduated centrifuge tube, add 70 % methanol to 12mL, ultrasonic extraction for 45min, temperature 40°C, centrifuge at 5000r / min for 15min, transfer the extract to a rotary evaporator, then add 70% methanol to a 15mL graduated centrifuge tube to 12mL, ultrasonic extraction for 30min, temperature 40°C , centrifuge at 5000r / min for 15 minutes, combine the two extracts, rotary evaporate to 2-5mL, transfer to a 10mL graduated tube, dilute with methanol, filter through a 0.45μm filter membrane, and perform qualitative and quantitative analysis on the filtrate according to 3. Chromatographic conditions , as a result, glycyrrhizic acid (dipotassium glycyrrhizinate) was detected, and the component collected for 16.445 minutes was glycyrrhizic acid (di...

Embodiment 2

[0074] The sample to be processed is the essence cream in cosmetics (sample number is HC20120366): Accurately weigh two parallel samples of the essence cream sample, each 1.0g (accurate to 0.01g) in a 50mL graduated centrifuge tube, add 70% methanol to 30mL , ultrasonic extraction for 45 minutes, temperature 40°C, centrifuge at 5000r / min for 15 minutes, transfer the extract to a rotary evaporator, then add 70% methanol to a 50mL graduated centrifuge tube to 30mL, ultrasonic extraction for 30min, temperature 40°C, 5000r / min Centrifuge for 15 minutes, combine the two extracts, rotary evaporate to 2-5mL, transfer to a 10mL graduated tube, dilute with methanol, filter through a 0.45μm filter membrane, perform qualitative and quantitative analysis on the filtrate according to 3. Glycyrrhetinic acid, the component collected for 28.936min is glycyrrhetinic acid, see image 3 .

[0075] Example 2 Glycyrrhetinic acid was detected in the essence cream, and the peak area was substituted...

Embodiment 3

[0077] The sample to be processed is essence milk in cosmetics (sample number is HC20120363): Accurately weigh two parallel samples of essence milk sample, each 0.7g (accurate to 0.01g) into a 50mL graduated centrifuge tube, add 70% methanol to 20mL , ultrasonic extraction for 45 minutes, temperature 40°C, centrifuge at 5000r / min for 15 minutes, transfer the extract to a rotary evaporator, then add 70% methanol to a 50mL graduated centrifuge tube to 20mL, ultrasonic extraction for 30min, temperature 40°C, 5000r / min Centrifuge for 15 minutes, combine the two extracts, rotary evaporate to 2-5mL, transfer to a 10mL graduated tube, dilute with methanol, filter through a 0.45μm filter membrane, perform qualitative and quantitative analysis on the filtrate according to 3. Dipotassium glycyrrhizinate (glycyrrhizic acid) and glabridin, the component collected for 16.457 minutes is dipotassium glycyrrhizinate (glycyrrhizic acid), and the component collected for 23.257 minutes is glabrid...

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Abstract

The invention relates to a method for measuring seven functional components of liquorice in cosmetics. The method comprises the following steps: adding 60 to 80 percent of methyl alcohol into a sample to be tested, performing repeated ultrasonic extraction and centrifugation, combining centrifugal supernate, adding the supernate into a rotary evaporating flask, performing rotary evaporation till constant volume is not exceeded, transferring the methyl alcohol and the sample into a graduated tube, metering the volume through methyl alcohol, filtering through a filter membrane, performing liquid chromatography measurement on filtrates, adopting a diode array detector, using octadecylsilane chemically bonded silica as a filler column, detecting at 190 to 400 nm, using an acetonitrile-phosphoric acid solution as a flowing phase, performing gradient elution, and collecting seven functional components which are liquiritin, isoliquiritin, glycyrrhizin, dipotassium glycyrrhizinate (glycyrrhizic acid), licochalcone A, glabridin and glycyrrhetinic acid. The method is high in resolution ratio and sensitivity, good in selectivity, short in time, simple in sample treatment method, and suitable for the micro analysis of seven functional components of liquorice in the cosmetics, and fills a gap of the measurement of seven functional components of liquorice in the cosmetics.

Description

technical field [0001] The invention relates to a method for simultaneously determining seven functional components of licorice, in particular to a method for determining seven active components of licorice in cosmetics. Background technique [0002] In recent years, the natural plant licorice has been widely used in various cosmetics as a functional ingredient, and has various functions such as whitening, sun protection, anti-inflammation, sebum regulation, hair growth and hair care. The growth of natural plant cosmetics far exceeds that of ordinary cosmetics, with an increase of 58% from 2008 to 2010. Among them, cosmetics containing Chinese herbal medicine ingredients are the fastest growing. International brand cosmetics have also begun to intervene in the field of Chinese medicine. At present, some Chinese herbal medicines ( Such as honeysuckle, wild chrysanthemum, ginseng, licorice, Shouwu and ginger). [0003] Licorice is a perennial herb of leguminous family. It is ...

Claims

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Application Information

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IPC IPC(8): G01N30/02G01N30/34G01N30/06
Inventor 陆军王嘉仡佟晓波庞燕军
Owner LIAONING PROVINCE INST FOR FOOD & DRUG CONTROL
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