344results about How to "Accurate quantitative analysis" patented technology

The invention relates to an infrared photoacoustic spectroscopy detection device and method for decomposed components of sulfur hexafluoride under partial discharge, belonging to the technical field of partial discharge on-line monitoring of SF6 gas insulating electrical equipment. The device of the invention mainly comprises an induction voltage regulator, a corona free experimental transformer, a partial discharge free protective resistor, a standard capacitive voltage divider, a non-inductive resistor, a GIS analog element, a wide-frequency high-speed ultrahigh-capacity digital storage oscillograph and an infrared photoacoustic spectroscopy system. In the method of the invention, infrared photoacoustic spectroscopy detection is carried out on the decomposed gas of SF6 under partial discharge in the GIS analog element by the device of the invention. The invention has high sensitivity, little gas consumption, multiple detection components and strong anti-jamming capability, can effectively detect SF6, CF4, SO2F2, SOF2, SO2, HF and the like as low as 0.01 muL/L, and is suitable for on-line detection. The invention can be widely used for detection of partial discharge decomposed gas of SF6 in the SF6 gas insulating electrical equipment, especially the GIS equipment.

The invention relates to a method for measuring alkaloid in tobacco and tobacco products, which is characterized by dissociating alkaloid from the tobacco and tobacco products by using sodium hydroxide solution, extracting the alkaloid from samples by using triethylamine/trichloromethane solution, and detecting content of five kinds of alkaloid with GC-MS ( Gas Chromatograph-Mass Spectrum) quantitative analysis. Compared with other methods for analyzing the alkaloid, the invention has the advantages that alkaloid is separated from the tobacco by DB-35MS chromatographic columns so as to separate nornicotine and myosmine in the tobacco and accurately quantify target matters; batch sampling is carried out on the separation of nicotinamide and other alkaloid so as to detect the nicotinamide with high content in the tobacco and meet the quantitative analysis of other alkaloid with low content; the standard working curves of standard matters are separately compounded so as to avoid influence on quantization of other alkaloid by alkaloid catabolite existing in the standard samples. The method is simple and convenient and has the advantages of good repeatability, high analyzing and measuring sensitivity, and accurate quantization.

The invention which relates to an automatic constant temperature type photoacoustic detection device for SF6 decomposed components and an experiment method thereof belongs to the technical field of SF6 gas insulation device partial discharge online monitoring. The device is characterized in that an automatic constant temperature system is arranged in present infrared photoacoustic spectrum monitoring systems; and according to the method, the device is utilized to detect the SF6 gas partial discharge decomposed components in a GIS (gas insulated substation) simulation element under automatic preset temperature constancy conditions. According to the invention, the influence of external environment can be eliminated, the gas components of SO2, CO2, CF4, SO2F2, SOF2 and the like with the concentrations of low to 0.01muL/L can be effectively detected, the accuracy, the detection precision and the stability are high, and performances of a solid state relay are stable, so the long-term effective working of the automatic constant temperature system can be guaranteed. The device which can be widely applied to the field of the SF6 gas insulation equipment partial discharge online monitoring is especially suitable for detecting the SF6 gas partial discharge decomposed components in the GIS.

The invention discloses a stepped method for detecting the concentration of a hydrogen peroxide solution. The stepped method includes the following steps that 1, a photothermal conversion material reacting with hydrogen peroxide is used for modifying a nanometer material containing a rare earth element, so that a functional rare earth nanometer material is obtained; 2, a series of hydrogen peroxide solution with the standard concentration reacts with the functional rare earth nanometer material to obtain a series of mixed solution, the luminous intensity, ultraviolet-visible light absorption intensity and photo-thermal heating temperature of the mixed solution are detected, and a standard curve of the luminous intensity, ultraviolet-light absorption intensity, heating temperature and concentration is drawn; 3, the hydrogen peroxide solution with unknown concentration reacts with the functional rare earth nanometer material to obtain a mixed solution, a detecting mode is determined, corresponding data is determined, and the concentration can be obtained through comparison with the standard curve. The detection limit is effectively lowered, and meanwhile the linear detection range is promoted. The material is simple, an instrument is low in price, and the stepped method can be used for detecting foods, medicine, living body samples and other samples.

The invention relates to construction of a portable purging and trapping sampling device of volatile formaldehyde, and a method for rapidly detecting volatile formaldehyde by surface enhanced Raman scattering (SERS) and application of the method. The portable purging and trapping sampling device of volatile formaldehyde comprises a gas path purging system and a sampling system. The volatile formaldehyde in an actual sample reacts with a derivatization reagent after being purged by high-purity nitrogen, an enhanced particle is added to a reactant and then the detection is carried out through a Raman spectrometer. The formaldehyde reacts with a phenol reagent under the action of ammonium ferric sulfate under the acidic condition to generate a blue-green compound, and an SERS signal of the blue-green compound is enhanced and is superior to that of the formaldehyde, so that the formaldehyde is subjected to indirect qualitation and quantitation through determining the blue-green compound. The portable purging and trapping sampling device adopts Au/SiO2 as an SERS base material, is suitable for determination of the volatile formaldehyde in complex samples such as aquatic products, foods and environment, and has the advantages of simpleness, rapidness, high selectivity, and the like, and is suitable for field detection.

An X-ray analysis apparatus including: a radiation source configured to irradiate an irradiation point on a sample with radiation; an X-ray detector configured to detect a characteristic X-ray emitted from the sample, and output a signal including energy information about the characteristic X-ray; an analyzer configured to analyze the signal; a sample stage configured to allow placement of the sample thereon; a shifting mechanism being capable of relatively shifting the sample on the sample stage and the radiation source and the X-ray detector with respect to each other; a height measuring mechanism being capable of measuring the height of the irradiation point on the sample; and a controller configured to control the shifting mechanism on the basis of the measured height of the irradiation point on the sample and adjust the distance of the sample with respect to the radiation source and the X-ray detector is used.

The invention discloses an interference-removing dry chemical quantitative test strip. The strip comprises a bottom liner, wherein the bottom liner is provided with a sample addition region and also a starting region; the sample addition region is fixed with interference-removing reagents and signal providing reagents; the starting region is fixed with test reaction starting reagents; and the sample addition region and the starting region are arranged on the test strip in a mode of maintaining a non-contact under a normal state and mutually contacting under a test state. The test method comprises the following steps of: adding a sample solution to be tested into the sample addition region, re-dissolving materials fixed on the sample addition region into the sample solution to be tested, and starting to perform a signal reaction to obtain a background signal value; and then enabling the starting region to contact with the sample addition region, re-dissolving materials fixed on the starting region, starting a test reaction, performing a second-time signal reaction on a product again, and estimating out a content of a biological enzyme to be tested according to a tested second-time characteristic value and the background signal value. The test method provided by the invention has the advantages of convenience for operation, low test cost, accurate test result and high test precision and can specifically be used in the alanine and aspartate aminotransferase quantitative test.

The invention discloses a method for confirming a photoetching technique window through accurate and quantitative defect detection. The method comprises the following steps: providing a wafer used for confirming the photoetching technique window; setting a benchmark chip in the wafer; distributing and arranging non-benchmark chips in the wafer according to a matrix of photoetching energy and focal length; successively exposing the benchmark chip and the non-benchmark chips; successively performing the defect detection on the exposed benchmark chip and the exposed non-benchmark chips; comparing the defect detection result of the exposed benchmark chip with the defect detection result of the exposed non-benchmark chips to obtain the confirmed photoetching technique window, wherein the benchmark chip is a chip used for determining the benchmark photoetching technique conditions. The method provided by the invention can be used for accurately and quantitatively analyzing a photoetching technique, and obtained photoetching technique window confirmation data have the very good accuracy and the accessible high readability.

The invention discloses a method for detecting alkaloids and nitrosamines in tobaccos simultaneously, and belongs to the technical field of tobacco chemical composition detection. The method comprises the working procedures of: sample extraction, purification, analysis and quantification. Firstly an internal standard solution and ultrapure water are added into tobacco leaf powder, extract liquor passes through a filter film after ultrasonic extraction, then methanol is added, protein is removed through centrifugation after shaking, and supernatant is subjected to liquid chromatography-mass spectrometry. After standard solutions of compounds to be detected with different concentration gradients are prepared by adopting a mixing preparation method, and the liquid chromatography-mass spectrometry is carried out, standard curves of instrument responses of the compounds to the actual concentrations of the solutions are drawn, curve equations are fitted, and the detection concentrations are calculated according to measured values of the instrument responses of the compounds to be detected and converted to the actual concentrations of the compounds to be detected in tobacco samples. The detection method provided by the invention can be used for carrying out the qualitative and quantitative analysis on alkaloids and nitrosamines in the tobacco samples simultaneously, and is easy and convenient to operate, good in reproducibility, high in analytic sensitivity and accurate in quantification.

The invention discloses a method for simultaneously detecting multiple anti-tumor drugs in a blood sample. A pretreated sample to be detected is detected by adopting ultra-high performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS). The pretreatment process comprises the following steps: adding serum into a mixed solution of methanol and acetonitrile, oscillating and centrifuging,taking out the centrifuged supernatant, drying, dissolving the dried powder into a methanol aqueous solution, and filtering to obtain a sample to be detected. The method can be used for simultaneously detecting 13 kinds of anti-tumor drugs such as methotrexate, 5-fluorouracil, apatinib, busulfan, carboplatin, cyclophosphamide, docetaxel, gemcitabine, imatinib, illinotecan, lenalidomide, oxaliplatin, paclitaxel and the like in blood.

The invention relates to a mass spectrum qualitative and quantitative analysis method for free fatty acids based on a double-derivatization technology. The mass spectrum qualitative and quantitative analysis method comprises the following steps: 1) a photochemical derivatization reaction of double bonds of unsaturated fatty acids; 2) an N,N-diethylethylenediamine derivatization reaction of carboxyl terminals of the fatty acids; 3) a qualitative method for precise structures of the fatty acids; and 4) a quantitative method for the fatty acids. The method disclosed the invention is used for precise qualitative and quantitative analysis of the free fatty acids in actual samples, so that the problems that double-bond positions of the unsaturated fatty acids, particularly polyunsaturated fattyacids, are difficult to authenticate and the fatty acids are low in ionization efficiency and poor in detection sensitivity under a negative ion mode are solved, and high-sensitivity and accurate qualitative and quantitative analysis for the free fatty acids in the actual samples is realized.

The invention provides an automatic detection method for various retinal focuses in a retinal OCT image. The automatic detection method comprises the following steps: acquiring a retinal 3D OCT image;carrying out detection and preliminary segmentation on the retina 3D OCT image by adopting multiple trained retina focus detection and segmentation models to obtain a focus area subjected to preliminary segmentation; and correcting the preliminarily segmented lesion area to obtain an accurate retinal lesion area. According to the automatic detection method, the retinal focus area in the retinal OCT image can be accurately detected, and various retinal focuses in the retinal OCT image can be quantitatively analyzed, and ideal robustness and accuracy are achieved.

The invention discloses an in-situ classification and identification method of marine phytoplankton by microscopic fluorescence spectral imaging, and further discloses an in-situ classification and identification device of the marine phytoplankton by the microscopic fluorescence spectral imaging, and aims at combining a fluorescence microscopy with spectral imaging techniques to develop rapid microscopic fluorescence spectrum detection techniques which can be applied to algae in marine environments, obtain image arrays of similar algae at different wavelengths, take three-dimensional coordinate values of spectrums embodied in colorimetry under different excitation wavelengths as additional information of algal images, and extract features from different images of a same species obtained under the different wavelengths and train a classifier to realize classification and identification and statistics of algae species with similar shapes inside a same class group. An imaging system is divided into a laboratory scheme and an underwater scheme. The two sets of systems take actual requirements which can be complementary and verified to each other into consideration under different conditions and at different precisions.

The invention provides a method for analyzing non-data-dependent acquisition mode mass spectral data, comprising the steps of mixing standard ones not present and heavily isotope-labeled among a plurality of analysis samples into samples under test; analyzing the samples by jointly using gas or liquid chromatography and mass spectrometry; acquiring the standard samples heavily isotope-labeled from data acquired in various DDA modes, and mass spectral information of ingredients in the samples under test, and making corresponding mass spectral database files; searching for mas spectral information of the standard samples heavily isotope-labeled from data acquired in various DIA modes; making mass spectral database files suitable for the analysis of data files acquired in each DIA mode; searching DIA data by using mass spectral data commercial software to obtain qualitative and quantitative results of the ingredients of each sample. The mass spectral DIA analysis method provided herein is a brand-new method, ingredients can be massively detected in samples, and the ingredients can be more accurately and quantitatively analyzed.

The invention relates to a method of detecting evaporable carbonyl compounds in cigarette filters by the solid-phase extraction and capillary gas chromatography. The cigarette filter is added with the Hydrochloric acidified 2,4-dinitrophenylhydrazine solution, for vibrating and extracting, then the extracted liquid is moved to C18 solid phase extraction columnella, eluted by cyclohexane, and the elutriant analyzed by GC, DB-5MS capillary chromato bar is adopted. The volume of sample injection is 1MuL. N2 is used as carrier gas in splitless sample injection. The flow rate of carrier gas is 1-2.5mL/min and the temperature of injection port is 200-250 DEG C. FID detector and the temperature of detector is 250 DEG C. The heating process is to keep initial temperature at 100 DEG C for 0-1min and heating rate at 15-20 DEG C /min, to 200 DEG C for 5min, then the heat rate from 4-8 DEG C /min, heating to 250 DEG C, keeps the temperature under 250 DEG C for 10min, quantitative analysis is according to the peak area. The invention lowers the operation request and can eliminate interference of nontaget compound which can separate formaldehyde, butanone, acrolein, crotonaldehyde, n-butylaldehyde, acetone, acetaldehyde from the cigarette filters effectively and quantitative analysis the seven evaporable carbonyl compounds in the cigarette filter accurately.

The invention provides a novel color-developing agent luminosity analytical method for measuring hydrogen cyanide content in mainstream smoke quickly, which comprises the following steps of: pretreating a sample in which hydrogen cyanide in the mainstream smoke is collected, adding a color-developing agent, and quantifying by a luminosity analytical method, wherein the color-developing agent is 4-nitrobenzaldehyde and o-dinitrobenzene; preparing potassium cyanide (KCN) solution which is added with the color-developing agent and has different concentrations to make a standard curve; and quantifying the standard curve by an external standard method, adding the color-developing agent in a cigarette absorption liquid sample, and performing photometry detection at wavelength 533 nm by an ultraviolet spectrophotometer. In the method adopting the color-developing agent containing 4-nitrobenzaldehyde-o-dinitrobenzene, the selectivity and sensitivity are high, a reaction is completed instantaneously and the interference resistant capacity is high. In addition, the method is simple in operation, cigarette sidestream smoke can be detected, the HCN in smoke environment also can be analyzed qualitatively and semiquantitatively, and the method is also suitable for field detection.

The invention discloses a method for separating and determining impurities in vitamin E and a preparation thereof with an HPLC (High Performance Liquid Chromatography) method. The method comprises the following steps: chromatograph condition: by taking octadecylsilane bonded silica gel as filler, a mixed solution of acetonitrile, alcohol and water as a mobile phase A and a mixed solution of acetonitrile, alcohol and methanol as a mobile phase B, performing gradient elution, wherein a detection wavelength is 260-310nm, a column temperature is 25-45 DEG C, a flow rate of each mobile phase is 0.5-2.0mL/min, and a sample feeding quantity is 20-150 microlitres; preparation of a sample solution, adopting a polar solvent to prepare a d1-alpha-tocopherol acetate peak identification control solution with the concentration capable of being 0.5-3mg/mL; and determination: pouring the solution in a high performance liquid chromatograph, and recording and analyzing a chromatogram. By utilizing the method provided by the invention, quantitative analysis of related substances of the vitamin E as a crude drug and the preparation thereof can be accurately performed, and thus the quality controllability of the vitamin E and the preparation thereof is ensured.

The invention discloses an method for simultaneously detecting 124 pesticide residues in tea leaves. The method is characterized by comprising the following steps: A) sample pre-treatment; B) mechanical testing solution treatment: rapidly performing a qualitative and quantitative determination analysis, taking an extract in A, and using acetonitrile for dilution and using liquid chromatogram-massspectrometry for determination; performing the accurate qualitative and quantitative determination analysis, adding high anhydrous sodium sulfate in an amino column, using an acetonitrile-toluene solution for prewashing the column before sample addition, when a liquid surface reaches top of sodium sulfate, rapidly absorbing an above mentioned sample extract to transfer to a purifying column, usingan acetonitrile-toluene solution for eluting pesticide, performing rotation condensation on all filtrates in water-bath at temperature of 45 DEG C to a nearly dry state, drying the material with nitrogen at the temperature of 30-35 DEG C, dissolving residues by an acetonitrile-aqueous solution, filtering the material through a 0.2 [mu]m microfiltration membrane, and performing liquid chromatogram-tandem mass spectrometry; and C) mass spectrometry: performing accurate qualitative and quantitative determination analysis on determination liquid in the step B) by a liquid chromatography-mass spectrometer.

The invention relates to a gas-chromatography filler capable of separating and analyzing hydrogen isotopes and a preparation method of the gas-chromatography filler and belongs to the technical field of functional materials. The material takes gamma-Al2O3 as a carrier. The synthesis method comprises the steps of loading a metal-organic skeleton compound to the surface of the gamma-Al2O3, carrying out washing and drying, and then, dipping the gamma-Al2O3 in an aqueous solution of a transition metal salt MXn, thereby obtaining a loaded type composite material. The gas-chromatography fixed-phase material is applied to the separation of hydrogen isotopes H2/D2, the separation effect is good, the quantitative analysis is accurate, and the material can be repeatedly used and is low in price; due to good mechanical properties, the recovery and reuse are very convenient.

The invention discloses a biosensor based on an aptamer and a manufacturing method and application thereof and belongs to the field of biochemical technique. According to the biosensor based on the aptamer, n-octadecyl mercaptan is automatically assembled on a gold film, the n-octadecyl mercaptan is combined with carboxyl group graphene oxide through intermolecular force, activating treatment is carried out on the carboxyl group graphene oxide to enable carboxyl to be converted into an active ester group, and an amido bond is formed through the active ester group and amino acid on molecule of the aptamer to enable the aptamer to be fixed on the surface of the carboxyl group graphene oxide. According to the biosensor based on the aptamer, the electrochemical activity is good, the aptamer can be well fixed, and the biosensor can be used for detection of various kinds of target protein and can be applied to the fields such as biomedicine, food safety and environmental monitoring. The manufacturing method of the biosensor based on the aptamer is simple and easy to operate. Due to the fact that hydroxyl and epoxy group which are on the graphene oxide are converted into the carboxyl due to the adoption of NaOH and ClCH2COONa, the COOH functional group content is high, the good electrochemical activity is achieved, and the aptamer can be well fixed.

The invention discloses a method for displaying M/A island structures inside pipeline steel, and belongs to the technical field of metallographic phase structure displaying and quantifying. Specifically, A sample is grinded and polished into a mirror face to be used later; 1g sodium metabisulfite is added into 100ml distilled water inside a beaker; 3.65g picric acid and 0.65g sodium hydroxide are added into 100ml absolute ethyl alcohol; the two kinds of solutions are mixed according to the proportion of 3: 2 and are heated and stirred to obtain the improved staining reagent; 2-4% of nitric acid ethyl alcohol solution is used for slightly corroding the prepared sample, and the sample is placed into the staining reagent to be immersed for 120-240 seconds and then is washed clean through the ethyl alcohol and is blown to be dried; an M/A island presents bright white and is very different from the surrounding structures in the gray level. The method is easy to operate, the corrosion time is short, and the area content, the size, the shape and other characteristic parameters of the M/A island in the structures can be fast, accurately and quantificationally analyzed through metallographic phase software.

The invention discloses a dry chemical quantitative test strip with interference removal, comprising a substrate, a sampling region which is provided with a fixed interference removing reagent and arranged on the substrate, and a promoter region which is provided with a fixed test reaction promoter and a fixed signal supplying reagent and is arranged on the substrate, wherein, the sampling region and the promoter region are distributed on the test strip under the conditions that: the sampling region keeps non-contact with the promoter region in normal state, and the sampling region contacts with the promoter region in test state. The test method comprises the following steps: adding a sample liquid to the tested in the sampling region, redissolving the substance fixed on the sampling region in the sample liquid to the tested, carrying out reaction for removing interference; then contacting the promoter region with the sampling region, redissolving the substance fixed on the promoter region, and carrying out reaction for test; carrying out signal reaction on the reaction product, and according to the tested signal value, calculating the content of biological enzyme to be tested. The test method has the advantages of simple operation, low test cost, accurate test result, and high test precision, and can be concretely applied for the quantitative test of alanine transaminase or aspartate transaminase.

The invention discloses a detection method of free and combined carboxy methyl lysine in milk and dairy products. According to the detection method, isotope dilution high performance liquid chromatography- tandem ion trap mass spectrometry is adopted to detect free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products. According to the detection method, using of ion-pairing agent is avoided; no derivatization pretreatment is needed; efficiency, accuracy, sensitivity, and reproducibility are high; linearity range is wide; the detection method possesses unique features in analysis of the content of free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products, can be used for rapid and accurate qualitative and quantitative analysis on free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products, and can be used for detection of free and combined carboxy methyl lysine in milk and dairy products preferably.