Analysis method for simultaneous determination method of 124 pesticide residues in tea leaves

Abstract

The invention discloses an method for simultaneously detecting 124 pesticide residues in tea leaves. The method is characterized by comprising the following steps: A) sample pre-treatment; B) mechanical testing solution treatment: rapidly performing a qualitative and quantitative determination analysis, taking an extract in A, and using acetonitrile for dilution and using liquid chromatogram-massspectrometry for determination; performing the accurate qualitative and quantitative determination analysis, adding high anhydrous sodium sulfate in an amino column, using an acetonitrile-toluene solution for prewashing the column before sample addition, when a liquid surface reaches top of sodium sulfate, rapidly absorbing an above mentioned sample extract to transfer to a purifying column, usingan acetonitrile-toluene solution for eluting pesticide, performing rotation condensation on all filtrates in water-bath at temperature of 45 DEG C to a nearly dry state, drying the material with nitrogen at the temperature of 30-35 DEG C, dissolving residues by an acetonitrile-aqueous solution, filtering the material through a 0.2 [mu]m microfiltration membrane, and performing liquid chromatogram-tandem mass spectrometry; and C) mass spectrometry: performing accurate qualitative and quantitative determination analysis on determination liquid in the step B) by a liquid chromatography-mass spectrometer.

Description

technical field [0001] The invention relates to a method for simultaneously determining 124 kinds of pesticide residues in green tea and belongs to the technical field of detection of pesticide residues in agricultural products. Background technique [0002] In recent years, Guizhou has given full play to its advantages in resources, intensified the adjustment of agricultural structure, and focused on tea and other advantageous and characteristic industries. In 2015, the province's tea planting area was 6.89 million mu, ranking first in the country, with a comprehensive output value of nearly 10 billion yuan. The quality and safety of tea is an important basis for ensuring the sustainable development of the tea industry in our province. The China Food and Drug Administration's notice on further strengthening the supervision of tea quality and safety [Food and Drug Administration [2017] No. 29] points out that the safety of raw materials entering the factory should be strict...

AI Technical Summary

Problems solved by technology

At present, the detection methods of pesticide residues in Chinese tea mainly include gas chromatography-flame photometry and gas chromatography-electron capture method, etc., but there are few detection methods for pesticide residues in green tea raw materials, resulting in the detection of many pesticide residues in green tea Blank, increasing the risk of pesticide residue detection in finished tea

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