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Method for analyzing non-data-dependent acquisition mode mass spectral data and application thereof

A technology of mass spectrometry data and acquisition mode, which is applied in the field of detection of biological and other sample components, can solve the problems of influx of non-specific mass spectrometry data, inaccurate DIA quantitative data, and reduction of sample components, etc., to achieve a small data search time window and improve comprehensive Sex and accuracy, precise results

Active Publication Date: 2017-01-04
PROTEINT (TIANJIN) BIOTECHNOLOGY CO LTD
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Problems solved by technology

The disadvantages of this DIA Library production method are: (1) Due to the large span of RT, the search time window needs to be expanded during the DIA data search process, causing a large amount of non-specific mass spectrometry data to flood in, resulting in inaccurate DIA quantitative data; ( 2) Even when the analyzed DIA is quite different from the previous DDA RT, the components in the sample will be missed, resulting in the reduction of DIA qualitative sample components

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  • Method for analyzing non-data-dependent acquisition mode mass spectral data and application thereof
  • Method for analyzing non-data-dependent acquisition mode mass spectral data and application thereof
  • Method for analyzing non-data-dependent acquisition mode mass spectral data and application thereof

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Embodiment Construction

[0032] The present invention will be further described below in conjunction with the accompanying drawings.

[0033] For methods for analyzing mass spectrometry data in data-independent acquisition mode, see figure 1 , including the following steps:

[0034] (1) Mixing heavy isotope-labeled standards that do not exist in multiple analytical samples into the tested sample;

[0035] (2) Utilize gas phase or liquid chromatography and mass spectrometry to analyze sample;

[0036] (2.1) adopt DDA mode to collect the mass spectrum information of all components in the tested sample and the heavy isotope-labeled standard; the mass spectrum information includes mass spectrum retention time (RT) and its mass spectrum characteristics;

[0037](2.2) adopt DIA mode to collect the mass spectrum information of all components in the tested sample and the heavy isotope-labeled standard; the mass spectrum information includes mass spectrum retention time (RT) and its mass spectrum characteris...

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Abstract

The invention provides a method for analyzing non-data-dependent acquisition mode mass spectral data, comprising the steps of mixing standard ones not present and heavily isotope-labeled among a plurality of analysis samples into samples under test; analyzing the samples by jointly using gas or liquid chromatography and mass spectrometry; acquiring the standard samples heavily isotope-labeled from data acquired in various DDA modes, and mass spectral information of ingredients in the samples under test, and making corresponding mass spectral database files; searching for mas spectral information of the standard samples heavily isotope-labeled from data acquired in various DIA modes; making mass spectral database files suitable for the analysis of data files acquired in each DIA mode; searching DIA data by using mass spectral data commercial software to obtain qualitative and quantitative results of the ingredients of each sample. The mass spectral DIA analysis method provided herein is a brand-new method, ingredients can be massively detected in samples, and the ingredients can be more accurately and quantitatively analyzed.

Description

technical field [0001] The invention belongs to the field of component detection of biological and other samples, and in particular relates to an analysis method for mass spectrometry data in a data-independent acquisition (DIA) mode, and also relates to mass spectrometry data collected at different time points and having different degrees of collection differences by the method Applications that can be analyzed simultaneously. Background technique [0002] Liquid phase and gas chromatography coupled with mass spectrometer (LC / GC-MS / MS) technology is developing rapidly, and it is the most advanced sensitive and accurate material composition analysis platform in the world today. Among them, the data-independent acquisition (Data-Independent Acquisition, DIA) mode developed in recent years can collect all the mass spectrometry data about the components of the sample in the sample without depending on the components of the sample to be tested. Collect without omission. Compar...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/88
CPCG01N30/88G01N2030/8831
Inventor 李捷唐琦
Owner PROTEINT (TIANJIN) BIOTECHNOLOGY CO LTD
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