In-situ classification and identification method and device of marine phytoplankton by microscopic fluorescence spectral imaging

A spectral imaging and micro-fluorescence technology, which is applied in the field of in-situ classification and identification of marine phytoplankton in micro-fluorescence spectral imaging and devices, can solve the problems of difficulty in automatic image classification and identification, difficulty in species identification, etc. Sensitive effect

Inactive Publication Date: 2017-11-24
WEIFANG UNIVERSITY
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  • Abstract
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  • Claims
  • Application Information

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Problems solved by technology

With the rapid development of various image feature extraction and classifier methods, the research on methods and algorithms for automatic classification and recognition of different forms of algae is a common research idea at present for the image information obtai

Method used

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  • In-situ classification and identification method and device of marine phytoplankton by microscopic fluorescence spectral imaging
  • In-situ classification and identification method and device of marine phytoplankton by microscopic fluorescence spectral imaging
  • In-situ classification and identification method and device of marine phytoplankton by microscopic fluorescence spectral imaging

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Embodiment 1

[0039] Example 1 A method for in-situ classification and identification of marine phytoplankton by microscopic fluorescence spectroscopy

[0040] Such as Figure 1-3 As shown, the present invention provides a method for in-situ classification and identification of marine phytoplankton by microscopic fluorescence spectroscopy, comprising the following steps:

[0041] Step 1, fluorescence microscopy imaging step;

[0042]Step 2, the feature extraction and classification recognition steps of the algae image;

[0043] Step 3, image chromaticity spectrum and fluorescence spectrum correlation analysis step;

[0044] Step 4, in-situ measurement to determine parameters related to sample collection.

[0045] in,

[0046] Step 1, fluorescence microscopic imaging step: including laboratory microscopic fluorescence spectrum imaging step,

[0047] Including laboratory microscopic fluorescent spectrum imaging steps: using xenon lamp as excitation light source, using Leica DMILM fluores...

Embodiment 2

[0057] Example 2: A method for in-situ classification and identification of marine phytoplankton by microscopic fluorescence spectroscopy

[0058] Such as Figure 4 As shown, the present invention provides a method for in-situ classification and identification of marine phytoplankton by microscopic fluorescence spectroscopy, comprising the following steps:

[0059] Step 1, fluorescence microscopy imaging step;

[0060] Step 2, the feature extraction and classification recognition steps of the algae image;

[0061] Step 3, image chromaticity spectrum and fluorescence spectrum correlation analysis step;

[0062] Step 4, in-situ measurement to determine parameters related to sample collection.

[0063] in:

[0064] Step 1, the fluorescent microscopic imaging step: including the underwater fluorescent microscopic imaging step;

[0065] The underwater fluorescence microscopy imaging step uses an in-situ microfluorescence spectroscopy imaging system, using pigtail-LEDs that can...

Embodiment 3

[0076] Example 3: A device for in-situ classification and identification of marine phytoplankton by microscopic fluorescence spectroscopy

[0077] Such as Figure 5 As shown, the present invention provides a microscopic fluorescence spectrum imaging marine phytoplankton in situ classification and identification device, comprising:

[0078] It includes an excitation light source system 1, a flow sample cell 2, and a CCD imaging system 3.

[0079] The excitation light source system 1 includes a light source device 11 and an optical system 12 .

[0080] The light source device 11 includes excitation light source, imaging and image acquisition system.

[0081] The laser light source adopts a xenon lamp as an excitation light source; the excitation light source adopts a surface excitation method. The planar excitation method is to simultaneously excite the entire observation area and detect the fluorescence signal of the entire area at the same time, with fast imaging speed, whi...

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Abstract

The invention discloses an in-situ classification and identification method of marine phytoplankton by microscopic fluorescence spectral imaging, and further discloses an in-situ classification and identification device of the marine phytoplankton by the microscopic fluorescence spectral imaging, and aims at combining a fluorescence microscopy with spectral imaging techniques to develop rapid microscopic fluorescence spectrum detection techniques which can be applied to algae in marine environments, obtain image arrays of similar algae at different wavelengths, take three-dimensional coordinate values of spectrums embodied in colorimetry under different excitation wavelengths as additional information of algal images, and extract features from different images of a same species obtained under the different wavelengths and train a classifier to realize classification and identification and statistics of algae species with similar shapes inside a same class group. An imaging system is divided into a laboratory scheme and an underwater scheme. The two sets of systems take actual requirements which can be complementary and verified to each other into consideration under different conditions and at different precisions.

Description

technical field [0001] The invention relates to a method and a device for in-situ classification and identification of marine phytoplankton by microscopic fluorescence spectrum imaging. Background technique [0002] The monitoring of species and quantity of phytoplankton is a routine basic work in marine scientific research. The exploration of fast, accurate and in-situ automatic identification and analysis methods of phytoplankton and the establishment of the system have important theoretical and practical significance; it can not only enrich the taxonomic research of phytoplankton, but also realize the change, occurrence, Real-time detection of development and extinction process, and can be widely used in marine ecological survey, environmental monitoring, aquaculture, red tide, geology and other fields. This is the common goal pursued by domestic and foreign counterparts. [0003] Among the various automatic analysis and identification methods of phytoplankton that have...

Claims

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Application Information

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IPC IPC(8): G01N21/64
CPCG01N21/64G01N2021/6417G01N21/6458G01N21/6486
Inventor 王春艳樊心民黄小东
Owner WEIFANG UNIVERSITY
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