144results about How to "Rapid Quantitative Analysis" patented technology

The invention discloses a kind of array sensor for gas and the preparing method. The sensor uses the aurum, platinum or argentum as electrode; the RuO2, MoSi2, W or Pt is as heating membrane material; the random one or several kinds of the ZnO, SnO2 or Fe2O, or ZnO, SnO2 or Fe2O3 and Pt0.1wt%-3wt%, Pd0.1wt%-3wt%, Ni1wt%-30wt%, TiO21wt%-30wt%, WO31wt%-40wt%, Co2O3 1wt%-15wt%, V2O51wt%-30wt%, Al2O31wt%-20wt%, MnO21wt%-30wt% is as the sensing layer material. It gets the gas sensing array on the alumina, aluminium nitride or silicon base sheet with anti high temperature, electric insulating and heat conducting by the screen printing technology. The gas sensing array is fixed on the basal seat by the axis to form the gas sensing array sensor by ultrasonic hot press welding electrode leg wire. The sensor has the low production cost, low power cost, good mechanism stability. Each unit has the good gas sensing character and the process is proper for the big batch production.

The invention relates to construction of a portable purging and trapping sampling device of volatile formaldehyde, and a method for rapidly detecting volatile formaldehyde by surface enhanced Raman scattering (SERS) and application of the method. The portable purging and trapping sampling device of volatile formaldehyde comprises a gas path purging system and a sampling system. The volatile formaldehyde in an actual sample reacts with a derivatization reagent after being purged by high-purity nitrogen, an enhanced particle is added to a reactant and then the detection is carried out through a Raman spectrometer. The formaldehyde reacts with a phenol reagent under the action of ammonium ferric sulfate under the acidic condition to generate a blue-green compound, and an SERS signal of the blue-green compound is enhanced and is superior to that of the formaldehyde, so that the formaldehyde is subjected to indirect qualitation and quantitation through determining the blue-green compound. The portable purging and trapping sampling device adopts Au / SiO2 as an SERS base material, is suitable for determination of the volatile formaldehyde in complex samples such as aquatic products, foods and environment, and has the advantages of simpleness, rapidness, high selectivity, and the like, and is suitable for field detection.

The invention discloses a method for detecting monocyclic aromatic pollutants in engine tail gas. The method comprises the following steps: collecting tail gas to be detected by using an engine tail gas sampling unit, and putting the active-carbon tail gas to be detected in a test tube as the sample; adding dichloromethane into the sample, putting the test tube in an ultrasonic extractor to carry out ultrasonic extraction, filtering the extract liquor, evaporating and setting to the marked volume to concentrate the extract liquor, and preserving at low temperature; carrying out chromatographic analysis on the extract liquor preserved at low temperature; and carrying out linear regression by using the concentration of monocyclic aromatics as the horizontal ordinate and the peak area as the vertical coordinate to calculate a standard operation curvilinear equation, substituting the chromatographic result into a standard operation curvilinear equation, and calculating the concentration of monocyclic aromatics in the sample. The invention can quickly and accurately carry out qualitative and quantitative analysis on monocyclic aromatics and has the advantages of high sensitivity and selectivity and good reproducibility; and the relative standard deviation does not exceed 6.5%, the detection limit is 0.003-0.009 mg / m3, and the recovery rate is 87.5-95.2%.

The invention relates to a method of detecting aflatoxin and pesticide residues in a peanut, in particular to a method of determining aflatoxin and pesticide residues in a peanut by ultra performanceliquid chromatography-quadrupole / high resolution mass spectrometry, which belongs to the field of analytical chemistry. A method of determining four kinds of aflatoxin and eleven kinds of pesticide residues in a peanut by ultra performance liquid chromatography-quadrupole / high resolution mass spectrometry adopts high performance liquid chromatography-quadrupole-electrostatic field orbitrap high resolution mass spectrometry to determine aflatoxin and pesticide residues in a peanut, a sample is extracted through an acetic acid-acetonitrile-water extract and is purified through a PSA and C18 silica gel mixed system, an aqueous solution containing 0.1% of formic acid and methanol are used in a positive ion mode to perform gradient elution on a mobile phase, and high resolution mass spectrometry qualitative and quantitative detection is carried out. The method is accurate, efficient and reproducible, and can simultaneously determine four kinds of aflatoxin and eleven kinds of pesticide residues in a peanut.

The invention discloses a simultaneous determination method for nicotine, 1,2-propylene glycol and glycerol in electronic cigarette smoke. The method comprises the following steps: smoking an electronic cigarette according to a smoking scheme; capturing the smoke by using a glass fiber filter; after extraction oscillation is performed on the fiber filter by adopting internal standard-containing organic solution, taking 1mL of solution in a 2mL chromatogram bottle; detecting extracted solution through a gas chromatography-hydrogen flame detector. According to the method, a DB-ALC1 (30m*0.32mm*1.8 mum) chromatographic column is adopted, so simultaneous analysis of high-concentration 1,2-propylene glycol and glycerol and low-concentration nicotine in the electronic cigarette smoke can be simultaneously analyzed; the internal diameter of the chromatographic column is 0.32 mm; feed detection of the high-concentration 1,2-propylene glycol and glycerol in the extracted solution is not overloaded; meanwhile the chromatographic peak tailing phenomenon of the 1,2-propylene glycol and glycerol with high polarity can be effectively improved by using the 1.8 mum thick liquid membrane column, so the quantification is more accurate.

The invention discloses a high-sensitivity quantitative analysis method based on gas pressure detection, which can be used for high-sensitivity quantitative detection on many targets including inorganic ions, micro-molecules, and bio-macromolecules, such as proteins, DNA, viruses, bacteria and cells. The high-sensitivity quantitative analysis method utilizes an enzyme or nano particles and the like to catalyze hydrogen peroxide to generate a lot of gas; when a detection signal of a target molecule is converted into a gas pressure intensity signal, signal amplification is realized; finally, pressure intensity change is converted into an electric signal by a barometer, and then the electric signal is read so as to finish high-sensitivity quantitative detection. The barometer and three different detection systems including a DNA hydrogel system, a functionalized DNA sensing system and an ELISA (Enzyme-Linked Immuno Sorbent Assay) system are utilized to prove the feasibility, wide applicability and reliability of the high-sensitivity quantitative analysis method.

A biomolecule analysis kit includes a reaction container configured to perform an enzymatic reaction, the reaction container including a base portion which has a container-shaped portion and a low-adsorption structural portion which is provided on at least the inner surface of the container-shaped portion, the low-adsorption structural portion having an adsorption rate lower than the base portion at which at least one of a sample which becomes a target of analysis in the enzymatic reaction and a reagent for the enzymatic reaction is adsorbed thereonto, wherein a signal resulting from the enzymatic reaction is configured to be detected when the enzymatic reaction is performed in the reaction container.

The invention discloses an in-situ classification and identification method of marine phytoplankton by microscopic fluorescence spectral imaging, and further discloses an in-situ classification and identification device of the marine phytoplankton by the microscopic fluorescence spectral imaging, and aims at combining a fluorescence microscopy with spectral imaging techniques to develop rapid microscopic fluorescence spectrum detection techniques which can be applied to algae in marine environments, obtain image arrays of similar algae at different wavelengths, take three-dimensional coordinate values of spectrums embodied in colorimetry under different excitation wavelengths as additional information of algal images, and extract features from different images of a same species obtained under the different wavelengths and train a classifier to realize classification and identification and statistics of algae species with similar shapes inside a same class group. An imaging system is divided into a laboratory scheme and an underwater scheme. The two sets of systems take actual requirements which can be complementary and verified to each other into consideration under different conditions and at different precisions.

The invention discloses a quick classification and identification method for Ganpu tea (orange pu-erh tea). The method comprises the following steps of measuring characteristic volatile organic compounds in different standard samples by using a gas-phase ion migration analysis method, to acquire response strength data of flavor compounds of different types of standard samples; clustering the response strength data of the standard samples by using a statistical analysis method, and building a classification and identification model; measuring the characteristic volatile organic compound in an unknown sample by using the gas-phase ion migration analysis method having the same parameter with the previous step, thus acquiring a sample fingerprint spectrum, and putting the sample fingerprint spectrum into the classification and identification model acquired in the previous step for analysis, so as to judge the category and authenticity of the sample. According to the method provided by theinvention, the flavor quality is quantified and standardized, and the classification and identification model is built, so that the Ganpu tea can be quickly, objectively and accurately assessed, the standardization of the quality of the Ganpu tea can be effectively ensured, the product quality and market competitiveness can be improved, and the blank that sensory analysis cannot be accurate and standard is filled.

The invention provides a novel color-developing agent luminosity analytical method for measuring hydrogen cyanide content in mainstream smoke quickly, which comprises the following steps of: pretreating a sample in which hydrogen cyanide in the mainstream smoke is collected, adding a color-developing agent, and quantifying by a luminosity analytical method, wherein the color-developing agent is 4-nitrobenzaldehyde and o-dinitrobenzene; preparing potassium cyanide (KCN) solution which is added with the color-developing agent and has different concentrations to make a standard curve; and quantifying the standard curve by an external standard method, adding the color-developing agent in a cigarette absorption liquid sample, and performing photometry detection at wavelength 533 nm by an ultraviolet spectrophotometer. In the method adopting the color-developing agent containing 4-nitrobenzaldehyde-o-dinitrobenzene, the selectivity and sensitivity are high, a reaction is completed instantaneously and the interference resistant capacity is high. In addition, the method is simple in operation, cigarette sidestream smoke can be detected, the HCN in smoke environment also can be analyzed qualitatively and semiquantitatively, and the method is also suitable for field detection.

The invention relates to a method for determining the content of hydrocarbons and oxygen-containing compounds in Fischer-Tropsch synthetic oil. The method comprises the following steps: separating theFischer-Tropsch synthetic oil through a solid-phase extraction method to obtain a hydrocarbon mixed component and an oxygen-containing compound mixed component; carrying out gas chromatography-massspectrometry analysis, determining a reference component in an obtained spectrogram, calculating a retention index of each peak, determining hydrocarbons or oxygen-containing compounds corresponding to each peak according to the retention indexes and a compound qualitative database, and obtaining the content of each hydrocarbon or oxygen-containing compound according to the peak area. Compared with the prior art, according to the invention, the method has the advantages that rapid separation of hydrocarbons and oxygen-containing compounds in the Fischer-Tropsch synthetic oil is realized through a solid phase extraction method, mutual interference of hydrocarbons and oxygen-containing compounds in the Fischer-Tropsch synthetic oil in retention time is effectively eliminated through GC-MS, and rapid qualitative and quantitative analysis of each component is realized in combination with qualitative advantages of GC-MS and a simple data processing algorithm.

The invention discloses a method for displaying M / A island structures inside pipeline steel, and belongs to the technical field of metallographic phase structure displaying and quantifying. Specifically, A sample is grinded and polished into a mirror face to be used later; 1g sodium metabisulfite is added into 100ml distilled water inside a beaker; 3.65g picric acid and 0.65g sodium hydroxide are added into 100ml absolute ethyl alcohol; the two kinds of solutions are mixed according to the proportion of 3: 2 and are heated and stirred to obtain the improved staining reagent; 2-4% of nitric acid ethyl alcohol solution is used for slightly corroding the prepared sample, and the sample is placed into the staining reagent to be immersed for 120-240 seconds and then is washed clean through the ethyl alcohol and is blown to be dried; an M / A island presents bright white and is very different from the surrounding structures in the gray level. The method is easy to operate, the corrosion time is short, and the area content, the size, the shape and other characteristic parameters of the M / A island in the structures can be fast, accurately and quantificationally analyzed through metallographic phase software.

The invention discloses a detection method of free and combined carboxy methyl lysine in milk and dairy products. According to the detection method, isotope dilution high performance liquid chromatography- tandem ion trap mass spectrometry is adopted to detect free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products. According to the detection method, using of ion-pairing agent is avoided; no derivatization pretreatment is needed; efficiency, accuracy, sensitivity, and reproducibility are high; linearity range is wide; the detection method possesses unique features in analysis of the content of free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products, can be used for rapid and accurate qualitative and quantitative analysis on free carboxy methyl lysine and carboxy methyl lysine combined with protein in milk and dairy products, and can be used for detection of free and combined carboxy methyl lysine in milk and dairy products preferably.

The invention discloses a sample treatment method adopting SERS to detect bacteria. The method comprises the following steps: 1) taking a to-be-detected sample, collecting a thallus, and dispersing with water to obtain a bacterial solution; 2) adding silver nitrate into the bacterial solution obtained in the step 1), uniformly mixing, then, adding sodium borohydride, and generating silver nanoparticles to obtain a sol in which the bacteria and the silver nanoparticles coexist; and 3) taking the sol obtained in the step 2), dropping the sol on a hydrophobic silicon wafer, and carrying out natural evaporation. The invention further discloses a method for detecting the bacteria by using a SERS-LIBS coupling technology. According to the sample treatment method in the invention, the reproducibility and stability of the Raman spectrum can be improved, and the method is good in application prospect.

The invention relates to a method for rapidly determining the content of hyaluronic acid in fermentation liquor by using near infrared spectroscopy in the technical field of determining the hyaluronic acid content in hyaluronic acid. The method comprises the following steps: selecting the hyaluronic acid fermentation liquor with same fermentation condition; determining the practical content of hyaluronic acid at regular intervals, determining the original near infrared spectroscopy of different batches of fermentation liquor by using a near infrared spectroscopy analyzer under the same temperature condition; pretreating the original near infrared spectroscopy by using a first order derivative+Norris5 site smooth method, in a wave band scope of 7312.7cm<-1>-10000cm<-1>, associating the practical content of hyaluronic acid in fermentation liquor with pretreated near infrared spectroscopy and establishing a mathematics model by using a partial least square method; taking the fermentationliquor with the hyaluronic acid content to be detected, determining the spectra of the near infrared spectroscopy and predicting the hyaluronic acid content according to the mathematics model. The method of the invention has the advantages of environmental protection without pollution, simple operation, and enables a rapid and reliable determination of the content of hyaluronic acid in fermentation liquor; the error is small.

The invention discloses a photoelectric double-pulse laser induced breakdown spectrograph, comprising a pulse laser, a focusing lens, a movable platform, a discharge electrode, an optical diode, a pulse time-delay controller, a high-voltage pulse power supply, an optical collection system of optical radiation, a monochromator or a spectrograph, a photoelectric conversion element, a data collection unit and an electronic computer. The invention also discloses a spectral analysis method of the photoelectric double-pulse laser induced breakdown spectrograph. The photoelectric double-pulse laser induced breakdown spectrograph can greatly enhance the spectral analysis sensitivity.

The invention provides an analysis method for electrochemical detection of lithium precipitation and a corresponding module device thereof, and the method comprises the following steps: charging a to-be-detected battery, then carrying out standing treatment, and collecting an electrochemical signal of the battery; characterizing whether the sample battery generates lithium precipitation or not inthe previous charging process by analyzing the collected electrochemical signal, and grading the lithium precipitation degree of the sample battery; and if the semi-quantitative analysis needs to be carried out, carrying out discharge treatment on the lithium-separated battery and collecting an electrochemical signal. The battery semi-quantitative lithium precipitation amount is obtained by analyzing the discharge and the previous charge electrochemical signal. According to the method, the defects of complicated operation, low precision, toxicity, harm and the like of the traditional method for analyzing lithium precipitation by disassembling the battery are overcome, and the lithium precipitation of the battery is quickly, accurately and quantitatively analyzed by utilizing an electrochemical means and a modular device.

The invention discloses an optical fiber LIBS(Laser Induced Breakdown Spectroscopy) detection device and a method used in a main pipeline of a nuclear power plant. The device comprises two parts of afiber LIBS detector and a master control detection system. The master control detection system is installed in a mater control room of the nuclear power plant, and the optical fiber LIBS detector enters the pipeline for detection, wherein the two parts are connected through transmission optical fibers and control signal lines. The master control detection system and the optical fiber LIBS detectorare connected through the high temperature resistant high-power transmission optical fibers and control signal lines, so that a remote online detection for a fixed point can be performed on a designated area of the inner wall of the main pipeline in the main control room of the nuclear power plant; the to-be-detected pipeline does not need to be pretreated, so that the inner wall of the pipelineis slightly damaged or even undamaged; and multiple elements can be rapidly and quantitatively analyzed at the same time, and the whole detection process is safe and reliable in operation and high inefficiency.

The invention discloses a method for detecting distribution and the content of residual austenite in low alloy structure steel. Water is used as cooling liquid, metallographic waterproof abrasive paper is used for rough grinding and fine grinding of a test surface of a sample, the sample subjected to fine grinding is polished, sodium metabisulfite, picric acid, sodium hydroxide, distilled water, hydrochloric acid and absolute ethyl alcohol are mixed, stirred and heated to prepare a corrosion reagent, the polished sample is subjected to electrolytic polishing treatment, the sample subjected toelectrolytic polishing treatment is placed into prepared corrosion solvents to remove interference phases, and distribution and the content of the residual austenite of the sample are detected by metallographic observation and representation. The method is simple in operation and short in polishing corrosion time. After the low alloy structure steel is corroded, a residual austenite structure in the low alloy structure steel can be clearly displayed. Characteristic parameters such as the content, the size, the shape and distribution of the residual austenite can be rapidly, accurately and quantitatively analyzed by metallographic software.

The invention provides a high-throughput detection device and method for rapid analysis of Raman spectrum. The high-throughput detection device for rapid analysis of the Raman spectrum comprises an optical-disc-shaped micropore plate, a rotary slide table and a sealing cover, wherein a plurality of micropores for accommodating samples are arranged on the optical-disc-shaped micropore plate, and the micropores are arranged in a uniform circular array mode from inside to outside on the optical-disc-shaped micropore plate; the optical-disc-shaped micropore plate is connected to the rotary slide table in a threaded mode; and the size of the sealing cover is matched with the size of the optical-disc-shaped micropore plate and is in sealing with the optical-disc-shaped micropore plate. The high-throughput detection method rapid analysis of the Raman spectrum can be widely applied to multi-throughput detection and analysis of food and environmental pollutants. The micropore plate is combinedwith the precision rotary slide table, the qualitative and quantitative analysis of different components are achieved by collecting SERS spectral data of each sampling point without repeatedly adjusting the focal length. The high-throughput rapid analysis of components of a plurality of samples to be detected can be achieved, and rapid on-site detection application is facilitated.

The invention discloses a primer for quickly and quantitatively detecting Wolbachia in tissues of a mosquito and a reagent box and a method thereof. According to the invention, different tissues containing Wolbachia are separated and obtained to extract Wolbachia genomic DNA; and then the Wolbachia DNA is subjected to Real-time PCR (Polymerase Chain Reaction) detection by using a specific PCR primer of Wolbachia. The realization of the invention is beneficial for quickly and quantitatively analyzing a large quantity of tissue samples containing Wolbachia, so that the distribution of Wolbachiain the tissues of the mosquito can be conveniently monitored in a large territorial scope on a large scale.

The invention discloses primers and a probe for rapidly and quantitatively detecting duck adenovirus type 4 and a detection method and application of the primers and the probe. The sequences of the primers are as shown in SEQ ID NO.1-2, and the sequence of the probe is as shown in SEQ ID NO.3. The fluorescent PCR method capable of rapidly and quantitatively detecting the duck adenovirus type 4 inthe clinical sample is established for the first time; the detection method is simple to operate, the whole operation process does not exceed 3 hours, the sensitivity is high, the specificity is good,the repeatability is good, quantitative analysis can be accurately and rapidly carried out with high flux, and the detection method is beneficial to popularization and application in clinical practice.

The invention relates to a method for determining wax contents in asphalt, and belongs to the field of road engineering asphalt quality detection. The method comprises the steps of: preparing a sample, collecting an infrared spectrum of the sample by using an infrared spectrometer, and obtaining an average spectrum figure through an ATR manner; selecting an appropriate spectral segment as a modeling interval, carrying out baseline correction processing and peak processing on the spectrum by utilizing relevant software, and carrying out regression or fitting analysis on data of the modeling interval by using a mathematic method so as to establish a regression model; and predicting wax content in the unknown sample by utilizing the model. The method is low in error rate and is capable of carrying out real-time, in-situ, non-destructive, rapid and quantitative analysis on asphalt wax contents.

The patent refers to the field of 'pharmaceutical preparations'. The invention discloses a method for measuring flavone glycoside compounds in herba epimedii, which relates to the field of herba epimedii measurement methods. The method comprises the following steps: (1) crushing leaves of herba epimedii, sieving the crushed material, placing the sieved material in a container, adding 50 to 70 percent aqueous solution of ethanol, extracting the flavone glycoside compounds, collecting supernate, filtering and obtaining samples to be analyzed; and (2) analyzing the obtained samples to be analyzed by using high-performance liquid chromatography and tandem mass spectrometry. The method has the advantages that: the flavone glycoside compounds in herba epimedii can be effectively and completely extracted; analysis is performed through the separation by ultra performance liquid chromatography and specific scanning by the tandem mass spectrometry; and thus, quick, accurate and quantitative analysis is realized.

The invention discloses application of a molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial in glutathione assay. The method comprises the following steps: a series of glutathione solutions with normal concentrations respectively react with the molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial, so that a series of mixed liquors are obtained. Under the irradiation of near-infrared light, the changes of fluorescence intensities are detected; after a standard curve of fluorescence and glutathione concentrations is drawn, glutathione solution with an unknown concentration reacts with the molybdenum-based heteropolyacid-modified rare-earth fluorescent nanomaterial, so that a mixed liquor is obtained; under the irradiation of near-infrared light, the change of fluorescence intensity is detected and compared with the standard curve, and thereby the concentration of the glutathione solution is obtained. The method can quickly, sensitively and portably carry out quantitative assay on the glutathione, such as the quantitative assay of food, medicines, living samples and the like, moreover, the method is easy to operate, the linear effect is good, the limit of detection is low, the cost is low, instruments are convenient to carry, and movable assay can be implemented.

The invention discloses a method for detecting a content of ethyl carbamate in soybean-flavor Baijiu. The method comprises a sample preprocessing step and an LC-MS detection step, wherein in the sample preprocessing step, a sample is prepared by the following steps: sucking 1mL of sample, placing the sample into a 10mL bottle, adding 100 microliters of 1000 microgram / L ethyl carbamate inner marker, ensuring the volume to 10mL by using distilled water, uniformly mixing, filtering by a 0.22 micrometer organic filter membrane, and performing the LC-MS determination for filtrate; and in the LC-MS detection step, detecting the content of the ethyl carbamate in the filtrate by using a liquid chromatography-mass spectrometer. In the detection method, the sample is directly loaded onto a machine after being diluted by using the distilled water and then filtered by the microporous filter membrane, so that the preprocessing process of the sample is simplified, and the recovery rate of the method and the stability of a detection result are improved.

The invention discloses a sea urchin-shaped MOFs@COFs core-shell structure material as well as a preparation method and application thereof, and relates to the technical field of synthetic materials. The sea urchin-shaped MOFs@COFs core-shell structure material disclosed by the invention is prepared from NH2-MIL-125 (Ti) and COFs; according to the COFs, an imine bond connecting structure of 2, 3, 5, 6-tetrafluoro p-dibenzaldehyde and 2, 4, 6-tri (4-aminophenyl)-1, 3, 5-triazine is used as a structural unit. The material presents a sea urchin-like morphology with an NH2-MIL-125 (Ti) nanowafer as a core and a COFs nanowire as a shell layer, the material can be used as an adsorbent and an auxiliary matrix to effectively enrich fluoroquinolone antibiotics in a complex environment and a biological liquid sample, and then surface-assisted laser desorption plasma mass spectrometry is used for analyzing, relatively high analysis speed and relatively high sensitivity are achieved.

The invention discloses a bisphenol A content detecting method relating to bisphenol A and provides a synchronous fluorescence spectrum and partial least squares combined bisphenol A content detecting method. The bisphenol A content detecting method comprises the following steps: (1) preparing a mixed standard solution as a sample calibration set and scanning a constant-wavelength synchronous fluorescence spectrum of the sample calibration set, wherein the mixed standard solution is composed of bisphenol A, phenol and octyl phenol in different concentration ratios; (2) establishing a quantitative calibration model between the content of the bisphenol A and the strength of the constant-wavelength synchronous fluorescence spectrum by using partial least squares; (3) predicting the artificially-synthesized sample calibration set by using the quantitative calibration model obtained in the step (2), and solving the linear fitting relationship between a predicted value of each sample and a known reference value; and (4) scanning the constant-wavelength synchronous fluorescence spectrum of the sample to be detected, and predicting through the quantitative calibration model to obtain the content of the bisphenol A in the sample to be detected. The bisphenol A content detecting method is good in effectiveness, high in reliability and good in predicting effect for the bisphenol A in the sample to be detected.

The invention provides an urban emergency capability assessment method based on an entropy weight matter element extension model, and belongs to the technical field of urban emergency capability assessment. The method comprises the following steps: 1, establishing an urban emergency capability evaluation index system; 2, determining the weight of each index by using an entropy weight method; 3, designing an expert scoring table; 4, performing scoring through experts in different industries, and performing statistics on scoring data; 5, establishing a matter element extension model, and evaluating the urban emergency capacity by using the model; and 6, performing same-level index sorting according to a result. The method can be used as a basic framework structure of urban emergency capability evaluation index systems in different regions, can comprehensively analyze related factors influencing the urban emergency capability, and can quickly, accurately and quantitatively analyze the urban emergency capability.

The invention relates to the field of biotechnology, and in particular, relates to a method for knocking out a pig GOT1 gene by a CRISPR / Cas9 system. The invention provides an sgRNA specifically targeting to the pig GOT1 gene, a CRISPR / Cas9 gene knock-out vector containing the sgRNA, a method for knocking out the pig GOT1 gene by the vector, and a detection method for the pig GOT1 gene. The CRISPR / Cas9 knockout vector provided by the invention can achieve high efficiency of the pig GOT1 gene knockout, significantly improve the construction efficiency of GOT1 gene knockout cells and GOT1 gene knockout pigs, and provide an effective method and basis for the function research and application of the pig GOT1 gene.