This invention relates to the field of
glucose detection technology, and discloses a method based on Au-MnO X A method for
glucose detection using
nanocomposite materials and its application. The
glucose detection method provided by this invention utilizes Au-MnO4. X The
nanocomposite material possesses a dual-
enzyme cascade catalytic activity similar to
glucose oxidase and
catalase, avoiding the drawbacks of poor stability, easy inactivation, and high preparation cost of natural enzymes. The material exhibits good stability and can be stored for a long time. The
oxygen bubbles generated by
catalysis can be directly observed with the
naked eye, and the bubble volume can be quantified by combining photography and
image processing software, eliminating the need for large instruments and simplifying the operation. The method demonstrates good sensitivity and selectivity, showing a good
linear relationship between bubble volume and concentration within the glucose concentration range of 4-8 mmol / L. It also exhibits excellent resistance to common interfering substances such as
fructose,
starch, and
sucrose, and can be directly used for rapid quantitative analysis of glucose in liquid samples such as beverages, blood, and body fluids, meeting the needs of on-site real-time detection.