The invention provides a method for measuring a water sulfide concentration through flow injection chemiluminescence. The method is performed according to the following steps of: 1. inputting a measured water sample into a water sample reaction chamber in a reaction chamber; 2. delivering ozone into the water sample reaction chamber in the reaction chamber, performing a gas-liquid phase reaction for 30-50s, carrying out ozone oxidation to the sulfide in the water sample so as to generate sulfur dioxide; 3. transporting the completely reacted measured water sample solution, which is mixed with phosphoric acid in a phosphoric acid pipeline; 4. then mixing the mixed solution mixed with the phosphoric acid with a potassium permanganate solution, and letting a photomultiplier tube detect a chemiluminescent signal generated during oxidation of the sulfur dioxide by potassium permanganate in the solution; and 5. allowing the photomultiplier tube to collect and amplify an optical signal emitted by a solution flowing through, converting the signal into an electric signal, which is then sent into a microcomputer data processing system, then quantizing the signal, and calculating the concentration of the sulfide in the water. The invention has the advantages of high sensitivity, wide linear range, rapidity, good reproducibility, as well as high degree of automation, etc.