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A method for measuring ddt in water by molecular imprinting selective fluorescence quenching method

A fluorescence quenching method and molecular imprinting technology, applied in the field of environmental chemical monitoring, can solve the problems of complicated analysis process, inability to work on site, secondary pollution, etc.

Active Publication Date: 2021-04-30
OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI
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Problems solved by technology

[0006] Aiming at the deficiencies in the prior art, the present invention provides a method for measuring DDT in water by the molecular imprinting selective fluorescence quenching method, which can solve the problems existing in the prior art that it cannot work on site, the analysis duration is long, the analysis process is complicated, the conditions are harsh, Large energy consumption, especially secondary pollution and other issues

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  • A method for measuring ddt in water by molecular imprinting selective fluorescence quenching method
  • A method for measuring ddt in water by molecular imprinting selective fluorescence quenching method

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Embodiment Construction

[0040] The present invention will be further described below in conjunction with the accompanying drawings and specific embodiments.

[0041] Such as figure 2 As shown, the device used in the present invention includes a three-way sampling valve 10, an enrichment column 11, a fluorescent flow cell 13, an excitation light source 15, a photoelectric detection device 16, a control device 18, a data processing device 17, a water sample pump 3, Buffer solution pump 5, sodium ethylenediamine tetramethylene phosphate pump 6, and eluent pump 9. Water sample 1 and blank solution 2 can be passed into the pipeline under the action of water sample pump 3, buffer solution 4 can be passed into the pipeline under the action of buffer solution pump 5, complexing agent 6 can be passed through under the action of complexing agent pump 7 Into the pipeline, the eluent 8 can be passed into the pipeline under the action of the eluent pump 9, the pumps used are peristaltic pumps, the three-way sam...

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Abstract

The invention discloses a method for measuring DDT in water by molecular imprinting selective fluorescence quenching method. First, sodium ethylenediaminetetramethylene phosphate forms a complex with DDT in water samples, and then enriches the complexes sequentially. , eluted, and finally detected by fluorescence technology. The formed complex has an obvious quenching effect on the fluorescence system, and the degree of fluorescence signal quenching has a linear relationship with the concentration of DDT in water. The method has the characteristics of being on-site, fast, simple and sensitive, and solves the problems existing in the prior art such as inability to work on-site, long analysis duration, complicated analysis process, harsh conditions, high energy consumption, and especially secondary pollution.

Description

technical field [0001] The invention belongs to the technical field of environmental chemical monitoring, and relates to a device and a method for measuring DDT in water bodies, in particular to a method for measuring DDT in water bodies by a molecular imprint selective fluorescence quenching method. Background technique [0002] DDT is also called DDT (four isomers), and its chemical name is bis-p-chlorophenyltrichloroethane. It is one of the important contents in water sample environmental monitoring. At present, the commonly used method for the determination of DDT in water is gas chromatography. The method is characterized by long duration, complicated analysis process, harsh conditions, large consumption of reagents, and the introduction of organic solvents harmful to human body to varying degrees in the pretreatment process of the method. , resulting in a large amount of waste of reagents and secondary pollution. At the same time, the method requires expensive and larg...

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/64
Inventor 高杨刘岩王昭玉王茜程岩曹煊孔祥峰郭翠莲曹璐张艳敏高楠
Owner OCEANOGRAPHIC INSTR RES INST SHANDONG ACAD OF SCI
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