A method for simultaneously determining arsenic forms of roxarsone, arsanilic acid and degradation products of same in soil

Abstract

The invention discloses a method for simultaneously determining arsenic forms of roxarsone, arsanilic acid and degradation products of same in soil, which comprises the following steps: adding a mixedextracting solution of NaH2PO4 and H3PO4 in a volume ratio of 9: 1 into a soil sample, uniformly mixing same, heating and extracting in a microwave digestion instrument, and centrifugally filtering the obtained extracting solution for high performance liquid chromatography-inductively coupled plasma-tandem mass spectrometry determination. The arsenic forms of roxarsone, arsanilic acid and degradation products of the same in the soil can be simultaneously and quickly determined; the determination accuracy, precision and stability all meet the requirements of environmental residue analysis anddetection; an adding standard recovery rate of roxarsone, arsanilic acid, monomethyl arsenic, dimethyl arsenic and inorganic arsenic ranges from 73.4% to 105.0%; the instrument detection limit rangesfrom 0.85 to 1.59 microgram / L, the precision ranges from 1.7% to 3.8%, and the requirement for polluted soil detection is met.

Description

Technical field: [0001] The invention relates to a method for simultaneously measuring the arsenic forms of roxarsone, arsanic acid and their degradation products in soil. Background technique: [0002] Roxarsone and arsanic acid are a class of organic arsine compounds, which are widely used in livestock and poultry breeding as feed additives because of their antibacterial, growth-promoting and livestock-coloring-promoting effects. Most of them cannot be absorbed by the intestines of animals. They are excreted from the animal feces and released into the soil environment in the form of livestock wastewater or animal feces, causing arsenic pollution in farmland soil and groundwater, and posing a serious threat to the health of the ecological environment. The toxicity of roxarsone and arsanic acid is not high, but they are easily transformed into forms such as methyl arsenic and inorganic arsenic in the soil environment, and the toxicity of inorganic arsenic is much higher than...

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Problems solved by technology

[0004] The purpose of the present invention is to provide a method for simultaneously measuring roxarsone, arsanic acid and its degradation product arsenic form in soil, which is fast, efficient, sensitive and accurate, and solves the problem of separation and detection in the prior art, long extraction time and easy to cause Interconversion and inaccuracy of arsenic forms

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