9results about How to "Fast elution" patented technology

The utility model provides a magnetic bead sorting NK immune cell preparation and extraction device which comprises a base, a control shell is upwards arranged on the base, a magnetic field shell is arranged in the middle of the front side of the control shell, and a plurality of magnetic field grooves are inwards formed in the front side of the magnetic field shell. Each magnetic field groove comprises a first-level magnetic field groove located on the upper portion and a second-level magnetic field groove located on the lower portion, the section of each first-level magnetic field groove is larger than that of the corresponding second-level magnetic field groove, and a first-level magnetic field electromagnet is arranged on the periphery of each first-level magnetic field groove in the magnetic field shell. A secondary magnetic field electromagnet is arranged on the periphery of each secondary magnetic field groove in the magnetic field shell; the chromatographic column is divided into a sample section, a first-stage chromatographic section matched with the first-stage magnetic field groove and a second-stage chromatographic section matched with the second-stage magnetic field groove from top to bottom. The utility model provides a grading magnetic bead sorting structure, which can effectively avoid the blocking phenomenon, effectively improve the sorting efficiency of cells and improve the final yield.

The present invention provides a column-based RNA extraction method, comprising the following steps: ① adding a lysate to a cell sample or tissue sample for lysing; ② adding anhydrous ethanol to the lysed cell sample or tissue sample, blowing and sucking, mixing evenly, transferring the above solution to an adsorption column, and placing the adsorption column in a collection tube, centrifuging, and pouring out the liquid in the collection tube; ③ adding a DNA enzyme and an elution buffer mixture to the adsorption column, standing; ④ adding a washing buffer to the adsorption column, centrifuging, and pouring out the waste liquid in the collection tube; putting the adsorption column back into the collection tube, centrifuging again, and pouring out the waste liquid in the collection tube; ⑤ transferring the adsorption column to a clean centrifuge tube, opening the cover and drying, and obtaining an adsorption film; ⑥ adding an elution buffer to the center of the adsorption film, standing; centrifuging, obtaining an RNA solution and preserving. The lysate, washing buffer, and elution buffer of the present invention use environmentally friendly and safe ingredients, and the extracted RNA has high concentration and high purity.

The invention relates to an analysis and detection method of lipid auxiliary materials for lipidosome, which adopts reversed-phase high performance liquid chromatography, and can detect one or more of phosphatidyl ethanolamine, hydrogenated soybean phosphatidylcholine, cholesterol and sodium distearoyl phosphatidyl glycerol for lipidosome under the same chromatographic condition. The analysis method disclosed by the invention is simple and rapid to operate, relatively low in cost, high in sensitivity, strong in specificity, good in repeatability and suitable for analysis and detection of other products with similar lipid auxiliary materials.

The present invention aims to solve the technical problem that the washing and regeneration process of the oil-containing resin in the carbon fiber oil production process requires the consumption of a large amount of water and hydrochloric acid solution, and provides a new method for treating the oil-containing agent ion exchange resin, which uses an ammonia solution and a mixed acid solution to treat the oil-containing resin. The method has the characteristics of low material consumption and good washing and regeneration effect.

This invention discloses a bismuth-containing composite, its preparation method, and its application in chloride ion elution. The bismuth-containing composite comprises bismuth oxide and a dopant element, which is one or more of titanium, nickel, copper, iron, cobalt, carbon, and nitrogen. As a dechlorination agent, this bismuth-containing composite, by selecting a semiconductor element as the dopant, can reduce the stability of the Bi-O covalent bonds in the sample, making them more susceptible to breakage under hydrogen ion attack, leading to structural collapse and the release of more bismuth ions, thus promoting the dechlorination reaction. Furthermore, after the bismuth-containing composite participates in the dechlorination reaction, the dechlorination product easily forms a heterojunction under light excitation during elution, allowing electrons to transfer to the introduced semiconductor, forming electron-hole pairs. The remaining holes on the bismuth oxychloride undergo photocorrosion, promoting the conversion of bismuth oxychloride to bismuth oxide, facilitating recycling, and improving subsequent dechlorination efficiency.

The utility model provides a chromatographic column and ionization source interface for GC-IMS (gas chromatography-ion mobility spectrometry) coupling technique, which comprises a chromatographic column, an ion migration tube and a GC-IMS interface for connecting the chromatographic column and the ion migration tube, one end of the GC-IMS interface is a screw sleeved on the chromatographic column, and the other end of the GC-IMS interface is a nut fixed on the ion migration tube; the ion migration tube comprises an ionization source, a reaction area, an ion gate, a migration area and a migration tube air outlet, and the migration tube air outlet is located on the side face of the first electrode of the ion migration tube; the chromatographic column is horizontally sealed and fixed on the axis of the ion migration tube, is axially connected with the ion migration tube through a GC-IMS interface and penetrates through the ionization source, a gas outlet of the chromatographic column is positioned in the reaction region and is positioned between the ionization source and the ion gate, and eluate of the chromatographic column enters the reaction region under the driving of carrier gas for ionization analysis. And meanwhile, the eluate is quickly blown out of the ion migration tube under the action of one-way drift gas. According to the technical scheme of the utility model, the problems of GC-IMS flow matching and sample residue are solved, and the GC-IMS response time resolution is improved.

The utility model discloses a kind of low pressure preparation chromatogram sample purification and automatic feeding device, it is related to the field of pharmaceutical synthesis experiment, and it includes: the upper end of extraction column pipe is set open, lower end is connected and is provided with lower interface, and extraction column pipe is filled with adsorption extraction filler layer above lower interface in;Adsorption extraction filler layer is flexible mechanical compressible material with communicating type porous;Vacuum evaporation heater is sleeved in the outside of extraction column pipe, for the target reaction mixture is removed by vacuum heating evaporation;Liquid channel is connected and is provided with in sample rod along the axial direction, and the outer periphery of one end of sample rod is circumferentially sleeved with sealing assembly;The one end of sample rod setting sealing assembly is pushed into extraction column pipe from upper end opening, for compressing adsorption extraction filler layer;The inner wall of extraction column pipe is tightly attached to the outer wall of sealing assembly, for sealing extraction column pipe. The scheme not only does not need to operate the silica gel micro powder of health threat, also greatly speeds up the purification speed of chemical reaction mixture.

The utility model belongs to the technical field of hydrogen sulfide waste gas purification, and particularly relates to a supergravity purification system for high-concentration hydrogen sulfide waste gas, which comprises an asphalt modification tower, a lye tank, a combustion furnace and a supergravity machine, the liquid outlet end of the lye tank is connected with the liquid inlet end of the supergravity machine, and the gas outlet end of the supergravity machine is connected with the gas inlet end of the combustion furnace. According to the utility model, the supergravity machine is additionally arranged, so that the contact surface area of waste gas and liquid is increased, the mass transfer coefficient and the reaction rate are effectively improved, and H2S and part of organic matters in non-condensable gas can be favorably eluted by alkali liquor, so that the content of sulfur in tail gas generated after the non-condensable gas is combusted is reduced.

This invention relates to the field of sample pretreatment technology, and in particular to a rapid extraction method for pesticides in water based on a confined liquid membrane. The method includes the following steps: preparing a composite substrate membrane comprising carbon nanofibers / carbon fibers and carbon nanofibers / carbon spheres; injecting ethanol as a confining solvent through the composite substrate membrane until droplets form beneath the membrane, thus completing the construction of the confined liquid membrane; passing a water sample containing the target pesticide through the confined liquid membrane at a set flow rate; using a desorption solvent at a set flow rate through the confined liquid membrane to elute the extracted target pesticide; collecting the eluent and performing quantitative analysis. This invention, through its ingenious confined liquid membrane design, successfully achieves rapid simultaneous extraction of pesticides with a wide polarity range and multiple categories from water while ensuring high extraction efficiency and good reproducibility.