52 results about "Magnetic Bead Separation" patented technology

The invention relates to the technical field of nano magnetic bead preparation, and discloses a magnetic bead continuous separation device. The magnetic bead continuous separation device comprises a magnetic bead separator, a magnetic field generating device and a driving device, the driving device is used for driving the magnetic field generating device to be close to or away from the side wall of the magnetic bead separator, the magnetic bead separator is hollow, one end of the magnetic bead separator is provided with a first liquid inlet and a second liquid inlet, and the other end of the magnetic bead separator is provided with a first liquid outlet and a second liquid outlet. According to the invention, a plurality of separation processes can be integrated in the same structure, so that the system configuration is simplified, and the requirements of automation and large-scale continuous treatment in the magnetic bead purification process are effectively met.

The invention relates to the technical field of nano magnetic bead preparation, and discloses a magnetic bead continuous separation system and a control method. The system comprises a magnetic bead separation device which is of a hollow pipe structure, the pipe wall of the magnetic bead separation device can be magnetized and demagnetized, one end of the magnetic bead separation device is provided with a first liquid inlet and a second liquid inlet, and the other end of the magnetic bead separation device is provided with a first liquid outlet and a second liquid outlet; the stock solution tank is connected to the first liquid inlet through a first flow channel; the waste liquid tank is connected to the first liquid outlet through a fifth flow channel, and a first magnetic bead sensor is arranged on the fifth flow channel; a liquid inlet of the product tank is connected to the second liquid outlet through a sixth flow channel, and a second magnetic bead sensor is arranged on the sixth flow channel; a liquid inlet of the stirring kettle is connected to the second liquid outlet through a seventh flow channel, and a liquid outlet of the stirring kettle is connected to the first liquid inlet through a second flow channel; a liquid outlet of the washing liquid tank is connected to the first liquid inlet through a third flow channel and is connected to the second liquid inlet through a fourth flow channel. The system has the advantage of meeting the requirements of continuous, automatic and infinite batch separation and purification of the magnetic beads.

The invention discloses a magnetic bead continuous separation process and device, and the process comprises the following steps: firstly applying a magnetic field to a magnetic bead separator, then continuously adding a stock solution into the magnetic bead separator, forming a magnetic bead capturing area and a magnetic bead separation area under the action of the magnetic field, capturing magnetic beads to the capturing area, discharging impurities to a waste liquid tank from a liquid outlet, and separating the impurities from the waste liquid tank. The concentration of the magnetic beads at the liquid outlet exceeds a set value; after the magnetic field is relieved, washing liquid is continuously added into the magnetic bead separator, and the magnetic beads are flushed out and collected into an intermediate tank; applying the magnetic field again, and continuously adding the collected liquid into the magnetic bead separator for separation again; after the magnetic field is relieved, washing liquid is injected into an inlet of the magnetic bead capturing area, so that the magnetic beads are flushed out until the concentration of the magnetic beads at a liquid outlet is lower than a set value; and circulating the steps until the treatment is finished. The process is automatically operated through a PLC (Programmable Logic Controller) control system. The magnetic bead separation device has the advantages that automatic feeding and discharging are achieved, multiple operation steps are completed in the same device, and multi-round repeated separation and automatic completion of magnetic beads are achieved.

The invention provides a lysosome extraction method. The lysosome extraction method comprises the following steps: (1) extracting cells; (2) tissue extraction; (3) crude separation of lysosome; and (4) separating the lysosome by magnetic beads. Through EDTA-free digestion optimization, lysis solution and buffer solution formula optimization, a filter membrane interception-magnetic bead purification combined technology, a double-antibody synergistic capture strategy and a competitive mild elution system, the four core problems of low purity, large activity damage, tedious operation and strong equipment dependence in a traditional lysosome separation technology are systematically solved. Experimental data show that the lysosome yield and functional integrity of the method are obviously superior to those of a density gradient centrifugation method, and the method is suitable for trace samples and conventional equipment and has high conversion value. In the future, the technical barrier can be further optimized through automation equipment integration and multi-dimensional function verification, and the application of the method in basic research and clinical diagnosis is promoted.

A solid-core ring-magnet having one or more cavities is provided. The magnet can have an overall cylindrical shape or a rectangular-prism shape. In either case, a portion of cavity walls of the magnet are ring shaped, causing the magnetic field lines to emanate from the magnet so that the bead formation is in the shape of a ring. A bead separation magnet having a discontinuous or segmented cavity wall is also provided. The segmented cavity wall causes bead formation to form in a segmented or gapped ring to allow for easier manual pipetting. Also provided are systems and kits having the inventive magnets. Methods of purifying a macromolecule using the inventive magnets are also provided.

The present application belongs to the technical field of fly ash separation, and particularly relates to a fly ash separation device based on fly ash extraction of glass microbeads. The present application comprises a magnetic bead separation assembly, a floating bead separation assembly and a carbon particle flotation assembly, the tailing channel of the carbon particle flotation assembly is communicated with the sink bead discharge pipeline; the discharge port of the magnetic bead separation assembly comprises a magnetic bead outlet and a dry material outlet, the dry material outlet is communicated with the negative pressure cavity of the negative pressure chamber, the water cavity of the negative pressure chamber is communicated with the water tank via the first pressure pump; the floating bead separation assembly comprises a floating bead flotation tank and a liquid inlet tank, the upper impeller and the lower impeller are arranged in the liquid inlet tank, the jet path of the nozzle is horizontally directed to the blade of the upper impeller, the nozzle is arranged at the bottom of the liquid inlet tank, and the overflow port of the floating bead flotation tank constitutes the floating bead outlet. The present application has the advantages of clear and reasonable process flow, effectively ensures the separation efficiency, and can sequentially separate the high-content magnetic beads, floating beads, carbon particles and sink beads according to the process flow, so as to facilitate subsequent processing.

A pretreatment method of an automatic analyzer, in which pretreatments of a plurality of substances to be measured can be performed by a series of treatments using a plurality of magnetic beads that can bind to the plurality of substances to be measured, is achieved. In the pretreatment method of an automatic analyzer, a magnetic bead is added to a sample containing a substance to be measured, the substance to be measured is bound to the magnetic bead, the magnetic bead is extracted from the sample, and the substance to be measured is separated from the magnetic bead by an eluate. A plurality of magnetic beads that bind to a plurality of types of substances to be measured are added to a sample, and the plurality of types of substances to be measured are extracted from the magnetic bead by an eluate.

The application relates to the technical field of nanometer magnetic bead preparation, and discloses a magnetic bead continuous separation system and a control method. The system comprises a magnetic bead separation device, which is in a hollow tube structure and can be magnetized and demagnetized at the tube wall, one end of the magnetic bead separation device is provided with a first liquid inlet and a second liquid inlet, the other end is provided with a first liquid outlet and a second liquid outlet, a raw liquid tank is connected to the first liquid inlet through a first flow channel, a waste liquid tank is connected to the first liquid outlet through a fifth flow channel, the fifth flow channel is provided with a first magnetic bead sensor, a product tank is connected to the second liquid outlet through a sixth flow channel, the sixth flow channel is provided with a second magnetic bead sensor, a stirring kettle is connected to the second liquid outlet through a seventh flow channel, and the liquid outlet of the stirring kettle is connected to the first liquid inlet through the second flow channel; a washing liquid tank is connected to the first liquid inlet through a third flow channel and connected to the second liquid inlet through a fourth flow channel. The system has the advantages of meeting the requirements of continuous separation and purification of magnetic beads in an automatic and unlimited batch mode.

An automated nucleic acid processing system includes a plurality of independent, asynchronously operating processing bays, each receiving a single biological sample. Each bay contains a robotic liquid handling module performing addition-only liquid transfers, a thermal cycling module executing programmable temperature profiles, a magnetic bead separation module, and a gripper mechanism transporting sample vessels between modules. A controller independently controls each processing bay, initiates sample processing without batch accumulation, and dynamically schedules workflow steps across all bays. Each processing bay receives a single-use closed consumable cartridge containing pre-measured reagents for nucleic acid extraction, library preparation, CRISPR-mediated depletion, and magnetic bead-based normalization. The system generates a sequencing-ready nucleic acid library from each biological sample without intermediate fluorometric quantification of nucleic acid concentration. An output interface provides a normalized sequencing library directly loadable onto a high-throughput sequencing instrument.

The invention discloses a precise construction strategy of a high-sensitivity DNA circulation inducible fluorescent nanorobot, which specifically comprises the following steps: A1, constructing a DNA nanostructure framework which is composed of a modularly designed recognition domain, a signal conversion domain and a circulation trigger domain, the recognition domain comprises an aptamer or a hairpin structure and is used for specifically combining with a target molecule, and the circulation trigger domain is used for specifically combining with the target molecule; the invention relates to the technical field of gene engineering. According to the precise construction strategy for the high-sensitivity DNA circulating inducible fluorescent nano-robot, through integration of a modular DNA tetrahedral framework and an enzyme digestion-strand displacement synergistic amplification system, cyclic utilization and signal exponential gain (such as CHA cascade amplification combined with CRISPR-Cas12a trans-cleavage) of a target object are achieved, the detection limit breaks through the 0.1 am level, and the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is greatly improved, so that the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is improved, and the accuracy of the high-sensitivity DNA circulating inducible fluorescent nano-robot is improved. Compared with a traditional single enzyme digestion or linear amplification method, the sensitivity is improved by at least three orders of magnitude. Meanwhile, time-resolved upconversion nanoparticles (UCNPs) are combined with a magnetic bead separation-lock type probe noise reduction technology.

The present invention eliminates the problems of conventional techniques caused by impurities or aggregates, and provides a new method for detecting an analyte or estimating the concentration thereof. The method for detecting an analyte or estimating the concentration thereof is characterized by comprising estimating the concentration of an analyte in a test sample using: a pore sensor having one pore, two chambers connected to both surfaces of the pore, and a pair of electrodes each installed in each of the two chambers; and a reaction reagent including adhesion-type magnetic beads having a magnetic bead surface to which a first adherent that specifically binds to the analyte is adhered, and adhesion-type detection beads having a non-magnetic bead surface to which a second adherent that specifically binds to the analyte is adhered. When the test sample and the reaction reagent are mixed to form a reaction liquid, bonding occurs between the adhesion-type magnetic beads and the adhesion-type detection beads via the analyte in accordance with the concentration of the analyte in the reaction liquid. A magnetic field is applied to a container accommodating the test sample including sandwich complexes, generated by the bonding, of an adhesion-type magnetic bead, the analyte, and an adhesion-type detection bead, to thereby fix the sandwich complexes including the adhesion-type magnetic beads in the container, and in this state, the reaction liquid is replaced with a cleaning liquid. Then, the cleaning liquid is removed, and further an eluate is introduced into the container to cause the adhesion-type detection beads to be released from the sandwich complexes into the eluate, and at the same time, the adhesion-type magnetic beads are fixed in the container by means of the magnetic field, thereby separating the adhesion-type detection beads from the adhesion-type magnetic beads. The eluate containing the separated adhesion-type detection beads is introduced into one of the two chambers of the pore sensor, and an electrolyte is introduced into the other of the two chambers, thereby providing electrical communication between the pair of electrodes. A voltage is applied to the pair of electrodes, and the number of pulse signals due to a current transient change caused in the pair of electrodes each time an adhesion-type detection bead passes through the pore is measured, thereby measuring the presence or absence or the concentration of the analyte in the test sample.

A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

The present invention relates to the field of sequence quality assessment, and in particular to a method for sequence quality assessment after magnetic bead-nucleic acid separation. The method comprises: sequencing the nucleic acid sample generated after magnetic bead separation to obtain a nucleic acid sequence set and its corresponding quality score; pre-processing the nucleic acid sequence set to obtain a pre-processed nucleic acid sequence set, calculating the integrity score of the nucleic acid sequence by a sequence integrity assessment algorithm; and calculating the sequence consistency score by a sequence consistency scoring algorithm; calculating a comprehensive quality score by combining the integrity score and consistency score of the nucleic acid sequence, and dividing the nucleic acid sequence into different quality levels according to the comprehensive quality score. The method solves the technical problem that the traditional method only relies on the quality score threshold when identifying contamination and errors in the sequence, but fails to comprehensively consider the base distribution uniformity and entropy value of the sequence.

The utility model discloses a magnetic separation frame which comprises a magnetic plate, a pipe frame and a base, a rotating mechanism for driving the magnetic plate to rotate is arranged on the base, and the pipe frame is movably connected with the magnetic plate. In the magnetic bead separation process of the sample tube, the rotating mechanism is additionally arranged on the base, and the tube frame and the magnetic plate are designed to be of a detachable connection structure, so that the magnetic frame has the characteristics that the installation is simple, waste liquid is convenient to pour, and a large number of samples can be quickly treated.

The invention relates to the field of molecular diagnosis equipment, and discloses an NGS library preparation instrument based on a digital microfluidic technology. Comprising a rack and a top plate fixedly arranged on the rack. And the substrate is provided with an opening penetrating through the thickness direction. And the chip bearing platform is arranged in the opening and is used for bearing the digital micro-fluidic chip. And the driving plate is fixed below the substrate, is positioned below the opening area, is provided with a pin array and is in contact with a bottom electrode of the chip. The pressing and releasing mechanism comprises a first pressing and releasing part and a second pressing and releasing part, elastic abutting force is applied through an elastic assembly, so that the digital micro-fluidic chip is electrically connected with the pin array, and the abutting force can be released to release the chip. A temperature control module; and a magnetic bead control module. The preparation instrument executes multiple steps of sample treatment, temperature control, magnetic bead separation and the like in the NGS library preparation process.

The present application relates to a kind of VSIG4 based on reversible immunological affinity magnetic bead separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation The isolated cells of the present application can reduce liver inflammation and injury, and effectively treat acute liver injury and acute liver failure. The isolation method of the present application is fast, mild and efficient, and the obtained cells are functional and suitable for adoptive cell therapy, which has a broad clinical application prospect.

The invention discloses a glyphosate fluorescence detection method based on RNase H cycle amplified magnetic bead-aptamer-Cas9 / deoxyribozyme, belongs to the technical field of environmental monitoring and biological detection, and can realize high-sensitivity and rapid quantitative detection of glyphosate residues in soil, water bodies and agricultural products. According to the method, the glyphosate is combined with an aptamer to release complementary DNA (cDNA) combined with the aptamer, then signal cascade amplification is realized through RNase H-mediated cDNA cyclic utilization, the anti-interference advantage brought by magnetic bead separation is combined, and the known characteristics of Cas9 endonuclease and DNAzyme in the aspects of nucleic acid recognition and cutting are used as signal amplification and transduction means, so that the detection sensitivity of the glyphosate is improved, and the detection sensitivity of the glyphosate is improved. Therefore, trace detection of glyphosate is realized. According to the invention, sensitive detection of other targets can be realized by replacing nucleic acid aptamers capable of specifically recognizing other targets and correspondingly adjusting the sequence of cDNA to maintain base pairing.

The invention relates to the technical field of sperm enrichment, in particular to a method for separating and enriching sperms by adopting IgY antibody immunomagnetic beads, which comprises the steps of sample carrier separation and sperm enrichment, and is characterized in that the sample carrier separation comprises the steps of digestion, low-position centrifugation and high-position centrifugation, and the sperm enrichment comprises the steps of immunomagnetic bead combination, magnetic separation and cleaning. The immunomagnetic bead coupled with the IgY antibody has the characteristics of strong specificity, high temperature resistance and acid and alkali resistance, belongs to a food-grade safety reagent, and is low in production cost, complete DNA can be detected only by injecting the immunomagnetic bead coupled with the IgY antibody for immunization of a hen for multiple times, separating and purifying the antibody after regular egg taking, wrapping the immunomagnetic bead coupled with the IgY antibody and tightening sperms, and 30 sperms. Compared with the prior art, an obvious experiment effect is achieved, laboratory operation is more convenient, the immunomagnetic beads can be combined with an instrument to achieve automatic operation, and sperm enrichment operation of mixed spot detection materials is greatly simplified.

The utility model discloses a microfluidic chip liquid absorption and magnetic bead attraction device and a liquid analysis system, and the device comprises a device body which is provided with a placing bayonet for placing a microfluidic chip and an injector fixing groove for fixing an injector; the liquid suction assembly enables the injector to be connected with the output port of the micro-fluidic chip, enables the input port of the micro-fluidic chip to be connected with liquid needing to flow in, and is used for adjusting the suction force of the micro-fluidic chip to the liquid needing to flow in; the magnetic bead attraction assembly is used for attracting magnetic beads, needing to flow into liquid, in the micro-fluidic chip to move; the detection assembly is used for detecting the liquid level and the magnetic bead position in the micro-fluidic chip and outputting a corresponding liquid level signal and a corresponding magnetic bead position signal; and the controller is used for receiving the liquid level signal, outputting a first driving signal to drive the liquid suction assembly to work, receiving the magnetic bead position signal and outputting a second driving signal to drive the magnetic bead attraction assembly to work. The utility model aims to realize accurate liquid suction and magnetic bead separation of the micro-fluidic chip at the same time.

The application relates to the technical field of nanometer magnetic bead preparation, and discloses a magnet ring surrounding pipe wall with assembled magnetic tiles, which comprises a round pipe-shaped embedded magnetic plate and a plurality of magnetic tiles, the inner diameter of the embedded magnetic plate is matched with the outer diameter of a magnetic bead separation pipe, a plurality of limiting through grooves are arranged on the pipe wall of the embedded magnetic plate in an array mode, the limiting through grooves are matched with the shapes of the magnetic tiles, and the magnetic tiles can be detachably embedded in the corresponding limiting through grooves. The application has the advantages of simple and beautiful structure, large magnetic attraction area, easy processing of the hexagonal magnetic tiles and adjustable magnetic field strength through cooperation of different magnetic tiles.

The utility model relates to the technical field of immunological separation, and provides a magnetic bead separator which comprises a base, an electromagnetic component, a positioning component and a fixing piece, the base is provided with a placing area for placing an elisa plate; the electromagnetic assembly comprises a circuit board and a plurality of electromagnetic elements, the circuit board is arranged in the placement area, the plurality of electromagnetic elements are electrically connected with the circuit board and are arranged on the circuit board in an array arrangement mode, and the circuit board is configured to be electrically connected with an external power supply; the positioning assembly is arranged on the base and surrounds the placement area, and the positioning assembly is used for limiting the placement posture of the elisa plate in the placement area, so that the plurality of electromagnetic elements and the plurality of slots in the elisa plate are arranged in a one-to-one correspondence manner; the fixing piece is arranged on the base and is used for fixing the elisa plate; the magnetic bead separator disclosed by the utility model not only facilitates the installation and fixation of the elisa plate, but also can ensure the separation effect of target magnetic beads, and is beneficial to ensuring the accuracy and stability of detection results in subsequent immune multivariate detection.

The invention discloses a preparation method of threose nucleic acid based on a self-pairing primer template. The method comprises the following operation steps: synthesizing a hairpin-shaped DNA template modified by biotin and hypoxanthine, and carrying out annealing treatment to realize template self-pairing; preparing a TNA sequence in a reaction system compatible with TNA extension and TNA cutting by taking the self-pairing DNA hairpin as a template; the TNA product and the template are separated through the magnetic beads, and the DNA template combined with the magnetic beads is recycled, so that the TNA product is recycled in multiple rounds of reactions, and the target TNA product is prepared in batches; the molecular weight of the product is detected through electrospray ionization mass spectrometry, and the prepared TNA is determined as an expected product. The invention provides a method for rapidly synthesizing a large amount of TNA sequences, the preparation period of TNA is shortened, and technical support is provided for application of TNA in the fields of biotechnology and biomedicine.

The utility model relates to the technical field of magnetic bead separation device, specifically a kind of magnetic bead separation device for separating adenovirus particles, comprising: device main body;Electromagnet, electromagnet is fixedly connected in device main body;Cover plate, cover plate is located in the upside of device main body by lifting assembly;Stirring mechanism, stirring mechanism is located in cover plate;Thread, thread is located in the lower part of the circumferential surface of device main body;And recovery bottle, recovery bottle is screw-connected on the surface of thread;By using the present device, the volume of device overall is small, easy to move, suitable for carrying, can be applied in clinical site, field research and emergency medical scene, and applicability is strong.

The invention discloses a carrier for acquiring life information and a method for applying the carrier, and belongs to the technical field of biomedicine. The method solves the problem that a required sample is difficult to quickly obtain during practical application of an existing method, realizes accurate extraction and detection of a life information carrier by integrating a magnetic bead separation technology and fluorescent quantitative detection, and performs intelligent classified storage and dynamic management based on data volume and data type, so that the life information carrier can be accurately extracted and detected during practical application. According to the method, quick retrieval and application can be performed in the specified storage area according to the data volume of the life information required to be applied, so that the management efficiency and retrieval speed of the life information are remarkably improved; the system can realize rapid biomarker analysis in disease diagnosis, supports dynamic data tracking in treatment monitoring, promotes accurate scheme formulation in personalized medical treatment, and achieves the comprehensive effects of improving medical detection efficiency, reducing storage cost and enhancing clinical application flexibility.

The invention provides magnetic bead separation equipment and a protein purification device.The magnetic bead separation equipment comprises a separation tank body and a magnetic field separator, the magnetic field separator is cylindrical, a magnetic field is formed on the radial inner side of the magnetic field separator, and the magnetic field separator can be arranged on the radial outer side of the separation tank body in a surrounding and sleeving manner; the magnetic field separator can move in the vertical direction relative to the separation tank body, so that the magnetic field separator moves between a first position and a second position, when the magnetic field separator is in the first position, the magnetic field separator and the separation tank body are located at different heights, and the separation tank body is located outside a magnetic field space formed by the magnetic field separator; when the magnetic field separator is in the second position state, the magnetic field separator and the separation tank body are located at the same height, the magnetic field separator surrounds the separation tank body, and the separation tank body is located in a magnetic field space formed by the magnetic field separator.

The invention belongs to the technical field of biological immunodetection and nano sensing, and particularly discloses a colorimetric / SERS (Surface Enhanced Raman Scattering) dual-mode detection method for a digestive tract tumor marker based on gold coral at platinum nano enzyme, which comprises the following steps: preparing an Au NCs at Pt nano enzyme probe labeled with a labeled antibody (Ab2); the method comprises the following steps: mixing and incubating a sample to be detected, a nano-enzyme probe and a magnetic bead coated with a capture antibody (Ab1) in an optimized incubation solution, and forming an immune sandwich compound through a specific immune reaction; carrying out magnetic separation and washing on the immune sandwich compound, and finally concentrating and fixing the volume to a small volume; adding an enzyme substrate TMB (Tetramethylbenzidine) solution, and catalyzing TMB to be oxidized into oxTMB by utilizing peroxidase-like activity of the nano-enzyme; and S5, observing the color change of the oxTMB by adopting a colorimetric method and detecting a characteristic Raman signal by adopting SERS (Surface Enhanced Raman Scattering), so as to realize rapid, high-sensitivity and high-specificity dual-mode detection on the CA19-9. According to the method, the detection sensitivity and efficiency are remarkably improved by utilizing the excellent catalysis and SERS enhancement dual effects of the Au NCs (at) Pt nano-enzyme and combining a magnetic bead separation and enrichment technology.

The present invention provides a small molecule probe for extracting and purifying mitochondria, as well as its preparation method and application. This approach addresses the current problem of using magnetic beads to extract mitochondria, which often results in ineffective separation of mitochondria from the beads and low biocompatibility. It provides an effective approach for mitochondrial implantation and future industrial development. The small molecule probe designed in the present invention includes a mitochondrial targeting group, a polyethylene glycol linker, a photo-controlled cleavage group, and an azide group, all bonded in sequence. The probe is connected to DBCO-magnetic beads via non-copper-catalyzed click chemistry. Upon irradiation with ultraviolet light at a wavelength of 365-400 nm, the photo-controlled cleavage group cleaves, thereby separating the mitochondria from the beads.

The invention relates to the technical field of biology, in particular to a lysis solution for rapidly extracting bacterial nucleic acid and a rapid nucleic acid extraction method based on the bacterial lysis solution and magnetic bead separation and enrichment. The lysate is prepared from 50m of Mtris (pH = 7.5), 20mM of EDTA (Ethylene Diamine Tetraacetic Acid), 0.7 M of NaCl, 1.5 M of guanidine isothiocyanate, 1M of guanidine hydrochloride, 0.2 percent of SDS (Sodium Dodecyl Sulfate), 1.5 percent of Tween-20, 1.5 percent of Triton X-100 and 1 percent of PEPPG F68. The extraction method comprises the steps of sample pretreatment and cracking, magnetic bead combination, magnetic bead washing, magnetic bead redissolution and magnetic bead separation, and can be used for treating Gram-positive bacterium and Gram-negative bacterium samples. Silicon hydroxyl magnetic beads with specific particle sizes are adopted, a 2mM NaOH solution is used for washing, and a Tris-HCl (pH = 7.9) solution is used for redissolving. The lysate can be used for preparing a reagent for rapidly extracting bacterial nucleic acid, the extraction method can be applied to bacterial detection or molecular biology experiments, the nucleic acid extraction time is effectively shortened to be within 10 minutes, and an efficient and reliable technical means is provided for related research and detection work.

To provide a technique for detecting biomolecules by releasing minute amounts of biomolecules into a region where the biomolecules can be effectively drawn in by electrical attraction from nanopores in order to lower a detectable concentration.SOLUTION: A biomolecule detection device provided herein comprises a laser element of a wavelength that generates heat in a nanomembrane and magnetic beads, and is configured to position the magnetic beads bound to biomolecules on the nanomembrane surface using magnetic force, then place the magnetic beads in a range near nanopores where electrical attraction acts, heat the magnetic beads with a laser to separate the biomolecules, and detect changes in an electrical signal when the biomolecules separated from the magnetic beads pass through the nanopores.SELECTED DRAWING: Figure 1

The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a simple magnetic bead separation nucleic acid extraction device which comprises a test tube placing plate, test tube placing holes are formed in the surface of the test tube placing plate, nucleic acid extraction test tubes are arranged in the test tube placing holes, and supporting plates are installed at the front end and the rear end of the lower surface of the test tube placing plate. The bottom end of the supporting plate is mounted on one side of the top end of the box body, a plate type electromagnet is arranged on one side of the nucleic acid extraction test tube and mounted on the outer wall of one side of a mounting plate, and the bottom end of the mounting plate is mounted at the top end of the box body. The nucleic acid extraction test tube is simple in overall structure, an operator can conveniently adsorb magnetic beads on the inner wall of one side of the nucleic acid extraction test tube to perform washing and elution operation, the operator does not need to manually operate a magnet to enable the magnetic beads to be adsorbed on the inner wall of one side of the nucleic acid extraction test tube, the operator can conveniently perform washing and elution operation, and the practicability is high.