33 results about "Magnetic Bead Separation" patented technology

A solid-core ring-magnet having one or more cavities is provided. The magnet can have an overall cylindrical shape or a rectangular-prism shape. In either case, a portion of cavity walls of the magnet are ring shaped, causing the magnetic field lines to emanate from the magnet so that the bead formation is in the shape of a ring. A bead separation magnet having a discontinuous or segmented cavity wall is also provided. The segmented cavity wall causes bead formation to form in a segmented or gapped ring to allow for easier manual pipetting. Also provided are systems and kits having the inventive magnets. Methods of purifying a macromolecule using the inventive magnets are also provided.

The present application belongs to the technical field of fly ash separation, and particularly relates to a fly ash separation device based on fly ash extraction of glass microbeads. The present application comprises a magnetic bead separation assembly, a floating bead separation assembly and a carbon particle flotation assembly, the tailing channel of the carbon particle flotation assembly is communicated with the sink bead discharge pipeline; the discharge port of the magnetic bead separation assembly comprises a magnetic bead outlet and a dry material outlet, the dry material outlet is communicated with the negative pressure cavity of the negative pressure chamber, the water cavity of the negative pressure chamber is communicated with the water tank via the first pressure pump; the floating bead separation assembly comprises a floating bead flotation tank and a liquid inlet tank, the upper impeller and the lower impeller are arranged in the liquid inlet tank, the jet path of the nozzle is horizontally directed to the blade of the upper impeller, the nozzle is arranged at the bottom of the liquid inlet tank, and the overflow port of the floating bead flotation tank constitutes the floating bead outlet. The present application has the advantages of clear and reasonable process flow, effectively ensures the separation efficiency, and can sequentially separate the high-content magnetic beads, floating beads, carbon particles and sink beads according to the process flow, so as to facilitate subsequent processing.

The application relates to the technical field of nanometer magnetic bead preparation, and discloses a magnetic bead continuous separation system and a control method. The system comprises a magnetic bead separation device, which is in a hollow tube structure and can be magnetized and demagnetized at the tube wall, one end of the magnetic bead separation device is provided with a first liquid inlet and a second liquid inlet, the other end is provided with a first liquid outlet and a second liquid outlet, a raw liquid tank is connected to the first liquid inlet through a first flow channel, a waste liquid tank is connected to the first liquid outlet through a fifth flow channel, the fifth flow channel is provided with a first magnetic bead sensor, a product tank is connected to the second liquid outlet through a sixth flow channel, the sixth flow channel is provided with a second magnetic bead sensor, a stirring kettle is connected to the second liquid outlet through a seventh flow channel, and the liquid outlet of the stirring kettle is connected to the first liquid inlet through the second flow channel; a washing liquid tank is connected to the first liquid inlet through a third flow channel and connected to the second liquid inlet through a fourth flow channel. The system has the advantages of meeting the requirements of continuous separation and purification of magnetic beads in an automatic and unlimited batch mode.

An automated nucleic acid processing system includes a plurality of independent, asynchronously operating processing bays, each receiving a single biological sample. Each bay contains a robotic liquid handling module performing addition-only liquid transfers, a thermal cycling module executing programmable temperature profiles, a magnetic bead separation module, and a gripper mechanism transporting sample vessels between modules. A controller independently controls each processing bay, initiates sample processing without batch accumulation, and dynamically schedules workflow steps across all bays. Each processing bay receives a single-use closed consumable cartridge containing pre-measured reagents for nucleic acid extraction, library preparation, CRISPR-mediated depletion, and magnetic bead-based normalization. The system generates a sequencing-ready nucleic acid library from each biological sample without intermediate fluorometric quantification of nucleic acid concentration. An output interface provides a normalized sequencing library directly loadable onto a high-throughput sequencing instrument.

A sample preparation workflow to facilitate deep N-glycomics analysis of human serum by capillary electrophoresis with laser induced fluorescence (CE-LIF) detection accommodates the higher sample concentration requirement of electrospray ionization mass spectrometry connected to capillary electrophoresis (CE-ESI-MS). A temperature gradient denaturing protocol is applied on amine functionalized magnetic bead partitioned glycoproteins to avoid precipitation. This also results in the free sugar content of the serum being significantly decreased which allows PNGase F mediated release of the N-linked carbohydrates. The liberated oligosaccharides were tagged with aminopyrene-trisulfonate, utilizing a modified evaporative labeling protocol. This workflow provides appropriate amounts of material for example for use in CE-ESI-MS analysis in negative ionization mode.

The utility model discloses a magnetic separation frame which comprises a magnetic plate, a pipe frame and a base, a rotating mechanism for driving the magnetic plate to rotate is arranged on the base, and the pipe frame is movably connected with the magnetic plate. In the magnetic bead separation process of the sample tube, the rotating mechanism is additionally arranged on the base, and the tube frame and the magnetic plate are designed to be of a detachable connection structure, so that the magnetic frame has the characteristics that the installation is simple, waste liquid is convenient to pour, and a large number of samples can be quickly treated.

The invention relates to the field of molecular diagnosis equipment, and discloses an NGS library preparation instrument based on a digital microfluidic technology. Comprising a rack and a top plate fixedly arranged on the rack. And the substrate is provided with an opening penetrating through the thickness direction. And the chip bearing platform is arranged in the opening and is used for bearing the digital micro-fluidic chip. And the driving plate is fixed below the substrate, is positioned below the opening area, is provided with a pin array and is in contact with a bottom electrode of the chip. The pressing and releasing mechanism comprises a first pressing and releasing part and a second pressing and releasing part, elastic abutting force is applied through an elastic assembly, so that the digital micro-fluidic chip is electrically connected with the pin array, and the abutting force can be released to release the chip. A temperature control module; and a magnetic bead control module. The preparation instrument executes multiple steps of sample treatment, temperature control, magnetic bead separation and the like in the NGS library preparation process.

The present application relates to a kind of VSIG4 based on reversible immunological affinity magnetic bead separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation + The present application provides a method for isolating VSIG4-positive macrophages, which uses reversible immunological affinity magnetic beads coupled with anti-VSIG4 antibodies to contact with a sample, and enriches VSIG4-positive macrophages by magnetic separation The isolated cells of the present application can reduce liver inflammation and injury, and effectively treat acute liver injury and acute liver failure. The isolation method of the present application is fast, mild and efficient, and the obtained cells are functional and suitable for adoptive cell therapy, which has a broad clinical application prospect.

The invention discloses a glyphosate fluorescence detection method based on RNase H cycle amplified magnetic bead-aptamer-Cas9 / deoxyribozyme, belongs to the technical field of environmental monitoring and biological detection, and can realize high-sensitivity and rapid quantitative detection of glyphosate residues in soil, water bodies and agricultural products. According to the method, the glyphosate is combined with an aptamer to release complementary DNA (cDNA) combined with the aptamer, then signal cascade amplification is realized through RNase H-mediated cDNA cyclic utilization, the anti-interference advantage brought by magnetic bead separation is combined, and the known characteristics of Cas9 endonuclease and DNAzyme in the aspects of nucleic acid recognition and cutting are used as signal amplification and transduction means, so that the detection sensitivity of the glyphosate is improved, and the detection sensitivity of the glyphosate is improved. Therefore, trace detection of glyphosate is realized. According to the invention, sensitive detection of other targets can be realized by replacing nucleic acid aptamers capable of specifically recognizing other targets and correspondingly adjusting the sequence of cDNA to maintain base pairing.

The application relates to the technical field of nanometer magnetic bead preparation, and discloses a magnet ring surrounding pipe wall with assembled magnetic tiles, which comprises a round pipe-shaped embedded magnetic plate and a plurality of magnetic tiles, the inner diameter of the embedded magnetic plate is matched with the outer diameter of a magnetic bead separation pipe, a plurality of limiting through grooves are arranged on the pipe wall of the embedded magnetic plate in an array mode, the limiting through grooves are matched with the shapes of the magnetic tiles, and the magnetic tiles can be detachably embedded in the corresponding limiting through grooves. The application has the advantages of simple and beautiful structure, large magnetic attraction area, easy processing of the hexagonal magnetic tiles and adjustable magnetic field strength through cooperation of different magnetic tiles.

The invention discloses a preparation method of threose nucleic acid based on a self-pairing primer template. The method comprises the following operation steps: synthesizing a hairpin-shaped DNA template modified by biotin and hypoxanthine, and carrying out annealing treatment to realize template self-pairing; preparing a TNA sequence in a reaction system compatible with TNA extension and TNA cutting by taking the self-pairing DNA hairpin as a template; the TNA product and the template are separated through the magnetic beads, and the DNA template combined with the magnetic beads is recycled, so that the TNA product is recycled in multiple rounds of reactions, and the target TNA product is prepared in batches; the molecular weight of the product is detected through electrospray ionization mass spectrometry, and the prepared TNA is determined as an expected product. The invention provides a method for rapidly synthesizing a large amount of TNA sequences, the preparation period of TNA is shortened, and technical support is provided for application of TNA in the fields of biotechnology and biomedicine.

The utility model relates to the technical field of magnetic bead separation device, specifically a kind of magnetic bead separation device for separating adenovirus particles, comprising: device main body;Electromagnet, electromagnet is fixedly connected in device main body;Cover plate, cover plate is located in the upside of device main body by lifting assembly;Stirring mechanism, stirring mechanism is located in cover plate;Thread, thread is located in the lower part of the circumferential surface of device main body;And recovery bottle, recovery bottle is screw-connected on the surface of thread;By using the present device, the volume of device overall is small, easy to move, suitable for carrying, can be applied in clinical site, field research and emergency medical scene, and applicability is strong.

The invention discloses a carrier for acquiring life information and a method for applying the carrier, and belongs to the technical field of biomedicine. The method solves the problem that a required sample is difficult to quickly obtain during practical application of an existing method, realizes accurate extraction and detection of a life information carrier by integrating a magnetic bead separation technology and fluorescent quantitative detection, and performs intelligent classified storage and dynamic management based on data volume and data type, so that the life information carrier can be accurately extracted and detected during practical application. According to the method, quick retrieval and application can be performed in the specified storage area according to the data volume of the life information required to be applied, so that the management efficiency and retrieval speed of the life information are remarkably improved; the system can realize rapid biomarker analysis in disease diagnosis, supports dynamic data tracking in treatment monitoring, promotes accurate scheme formulation in personalized medical treatment, and achieves the comprehensive effects of improving medical detection efficiency, reducing storage cost and enhancing clinical application flexibility.

The invention provides magnetic bead separation equipment and a protein purification device.The magnetic bead separation equipment comprises a separation tank body and a magnetic field separator, the magnetic field separator is cylindrical, a magnetic field is formed on the radial inner side of the magnetic field separator, and the magnetic field separator can be arranged on the radial outer side of the separation tank body in a surrounding and sleeving manner; the magnetic field separator can move in the vertical direction relative to the separation tank body, so that the magnetic field separator moves between a first position and a second position, when the magnetic field separator is in the first position, the magnetic field separator and the separation tank body are located at different heights, and the separation tank body is located outside a magnetic field space formed by the magnetic field separator; when the magnetic field separator is in the second position state, the magnetic field separator and the separation tank body are located at the same height, the magnetic field separator surrounds the separation tank body, and the separation tank body is located in a magnetic field space formed by the magnetic field separator.

The invention belongs to the technical field of biological immunodetection and nano sensing, and particularly discloses a colorimetric / SERS (Surface Enhanced Raman Scattering) dual-mode detection method for a digestive tract tumor marker based on gold coral at platinum nano enzyme, which comprises the following steps: preparing an Au NCs at Pt nano enzyme probe labeled with a labeled antibody (Ab2); the method comprises the following steps: mixing and incubating a sample to be detected, a nano-enzyme probe and a magnetic bead coated with a capture antibody (Ab1) in an optimized incubation solution, and forming an immune sandwich compound through a specific immune reaction; carrying out magnetic separation and washing on the immune sandwich compound, and finally concentrating and fixing the volume to a small volume; adding an enzyme substrate TMB (Tetramethylbenzidine) solution, and catalyzing TMB to be oxidized into oxTMB by utilizing peroxidase-like activity of the nano-enzyme; and S5, observing the color change of the oxTMB by adopting a colorimetric method and detecting a characteristic Raman signal by adopting SERS (Surface Enhanced Raman Scattering), so as to realize rapid, high-sensitivity and high-specificity dual-mode detection on the CA19-9. According to the method, the detection sensitivity and efficiency are remarkably improved by utilizing the excellent catalysis and SERS enhancement dual effects of the Au NCs (at) Pt nano-enzyme and combining a magnetic bead separation and enrichment technology.

The invention relates to the technical field of biology, in particular to a lysis solution for rapidly extracting bacterial nucleic acid and a rapid nucleic acid extraction method based on the bacterial lysis solution and magnetic bead separation and enrichment. The lysate is prepared from 50m of Mtris (pH = 7.5), 20mM of EDTA (Ethylene Diamine Tetraacetic Acid), 0.7 M of NaCl, 1.5 M of guanidine isothiocyanate, 1M of guanidine hydrochloride, 0.2 percent of SDS (Sodium Dodecyl Sulfate), 1.5 percent of Tween-20, 1.5 percent of Triton X-100 and 1 percent of PEPPG F68. The extraction method comprises the steps of sample pretreatment and cracking, magnetic bead combination, magnetic bead washing, magnetic bead redissolution and magnetic bead separation, and can be used for treating Gram-positive bacterium and Gram-negative bacterium samples. Silicon hydroxyl magnetic beads with specific particle sizes are adopted, a 2mM NaOH solution is used for washing, and a Tris-HCl (pH = 7.9) solution is used for redissolving. The lysate can be used for preparing a reagent for rapidly extracting bacterial nucleic acid, the extraction method can be applied to bacterial detection or molecular biology experiments, the nucleic acid extraction time is effectively shortened to be within 10 minutes, and an efficient and reliable technical means is provided for related research and detection work.

To provide a technique for detecting biomolecules by releasing minute amounts of biomolecules into a region where the biomolecules can be effectively drawn in by electrical attraction from nanopores in order to lower a detectable concentration.SOLUTION: A biomolecule detection device provided herein comprises a laser element of a wavelength that generates heat in a nanomembrane and magnetic beads, and is configured to position the magnetic beads bound to biomolecules on the nanomembrane surface using magnetic force, then place the magnetic beads in a range near nanopores where electrical attraction acts, heat the magnetic beads with a laser to separate the biomolecules, and detect changes in an electrical signal when the biomolecules separated from the magnetic beads pass through the nanopores.SELECTED DRAWING: Figure 1

The utility model belongs to the technical field of nucleic acid extraction equipment, and particularly relates to a simple magnetic bead separation nucleic acid extraction device which comprises a test tube placing plate, test tube placing holes are formed in the surface of the test tube placing plate, nucleic acid extraction test tubes are arranged in the test tube placing holes, and supporting plates are installed at the front end and the rear end of the lower surface of the test tube placing plate. The bottom end of the supporting plate is mounted on one side of the top end of the box body, a plate type electromagnet is arranged on one side of the nucleic acid extraction test tube and mounted on the outer wall of one side of a mounting plate, and the bottom end of the mounting plate is mounted at the top end of the box body. The nucleic acid extraction test tube is simple in overall structure, an operator can conveniently adsorb magnetic beads on the inner wall of one side of the nucleic acid extraction test tube to perform washing and elution operation, the operator does not need to manually operate a magnet to enable the magnetic beads to be adsorbed on the inner wall of one side of the nucleic acid extraction test tube, the operator can conveniently perform washing and elution operation, and the practicability is high.

Analysis of cell populations for both research and clinical applications such as cell therapy is often performed on whole blood that is close to 24 hours old. The reason for this is that the analysis site is removed from the blood draw site so that blood must be shipped overnight to the analysis site. During the 24-hour shipping period, granulocytes and other blood components break down into undesirable cell debris including nucleic acids that are released into the blood. Such debris is known to interfere with immunological assays and with thawing following freezing of samples. The present invention solves this issue by removing granulocytes (and optionally other components) using an anti-CD15 molecule (or other appropriate molecule) bound to metallic magnetic particles. Following mixing, the granulocytes (or other components) are removed and prepared for shipment to the analysis site or for other reasons.

The utility model discloses a multiplex fluorescence immunomagnetic bead separation reagent card, and relates to the technical field of biological detection. The reagent card comprises an upper cover and a shell, wherein the upper cover is provided with a sample adding hole; the lower part of the sample adding hole is connected with a first microfluidic pipeline arranged in the shell; a reaction bin, a detection bin, a quality control bin, a first capturing bin and a second capturing bin are also arranged in the shell; the first microfluidic pipeline is communicated with the reaction bin; the reaction bin connects the quality control bin and the first capture bin in series through a second microfluidic pipeline; the reaction bin connects the detection bin and the second capture bin in series through a third microfluidic pipeline; each detection bin is communicated with the corresponding second capture bin through an independent third microfluidic pipeline; and magnetic strips are arranged in the first capturing bin and the second capturing bin. According to the scheme, a to-be-detected sample only needs to be dropwise added into the sample adding hole, filtering treatment, mixing, reaction and multi-item detection of the sample can be achieved, different target objects are shunted through the independent third microfluidic pipeline, and the detection sensitivity is improved.

The invention discloses a four-channel digital micro-fluidic chip for library construction, which comprises a public reagent injection area, a temperature control area, a magnetic bead mixing area, a magnetic bead separation area and a waste liquid area, and realizes parallel four channels and low connecting line number through electrode connecting line multiplexing and symmetrical layout; injection is performed according to a volume shunting strategy, magnetic bead separation is completed in a separation area under the action of a magnetic control system along a set flow direction, and an amplification reaction is completed in a temperature control area. The waste liquid area adsorbs waste liquid to reduce the pollution risk. The chip and the method are adaptive to various library building volumes and reaction systems, and have the advantages of high throughput, low volume, low pollution, easiness in integration and the like.

The present application relates to a kind of rapid in-situ detection technology based on the interaction between photocatalysis-based nanoparticles and protein, a method for separating and extracting protein interacting with nanoparticles, the method steps are as follows: preparation contains photocatalysis probe and labeled reaction substrate nanoparticles, or preparation contains photocatalysis probe nanoparticles and labeled reaction substrate is added to the sample containing protein;2) the nanoparticles of step 1 and the sample containing protein are contacted to form the interaction between nanoparticles and protein;the labeled reaction substrate in nanoparticles is activated by the laser irradiation of the wavelength corresponding to photocatalysis probe, and the protein interacting with nanoparticles is obtained;3) the labeled protein interacting with nanoparticles is separated using streptavidin-coated magnetic beads.

The invention provides a method for separating and extracting amniotic mesenchymal stem cells from a placenta. The method comprises the following steps: S1, pretreating the placenta; s2, amniotic membrane tissue separation; s3, tissue crushing; s4, enzymolysis digestion: softening the fragments, and mixing the softened fragments with a compound enzyme solution according to a mass-volume ratio of 1: (8-12) to obtain an enzymolysis product; s5, centrifugal separation: performing gradient centrifugation on the enzymolysis product to obtain cell precipitate; s6, cell culture: inoculating cell precipitates obtained by centrifugation to a compound culture medium; and step S7, immune sorting: separating target cells by adopting CD90 antibody coated magnetic beads. According to the method disclosed by the invention, three defects of cell injury, insufficient purity and low yield are synchronously overcome through a softening liquid protection mechanism, multi-enzyme synergistic dissociation, gradient centrifugal layering and other core technologies; and standardized parameter control (such as thickness cutting and temperature gradient) ensures the process reproducibility, and provides high-activity and high-purity functional stem cells for clinical application.

The invention relates to the technical field of gynaecology and obstetrics, and discloses a model for predicting the PE risk of pregnancy in early and middle pregnancy, which comprises the following steps: collecting a sample from peripheral blood of a pregnant woman, and separating plasma; the method comprises the following steps: extracting free DNA from plasma by using a magnetic bead separation method, and performing purification and quantitative treatment; performing high-throughput sequencing on the extracted free DNA to obtain sequencing data; through bioinformatics analysis, sequencing data is compared to a human genome, and a gene sequence related to PE is identified; and on the basis of the identified biomarker, in combination with clinical data of the pregnant woman, constructing a model for predicting the PE risk. According to the method, the prediction model is constructed by combining the free DNA sequencing data and the clinical data, so that the accuracy and the stability of predicting the PE risk can be improved. And gene sequences and variations related to PE can be identified through bioinformatics analysis and genome data comparison, so that more reliable prediction information is provided.

The present invention provides a novel method for determining a long-chain non-coding ribonucleic acid interaction protein. The present invention provides a fusion protein formed by BASU and dCasRx, a mammalian expression vector for expressing said fusion protein. The method for determining the lncRNA interaction protein according to the present invention comprises: co-transfecting a mammalian expression vector that expresses the fusion protein and a gRNA that specifically targets the target lncRNA into target cells, thereby BASU specifically biotin-labeling effector proteins nearby; isolating the biotinylated proteins by using a streptavidin affinity coupled magnetic bead and then eluting, and digesting by trypsin and quantitatively analyzing by a label-free mass spectrometry. The present invention can highly credibly determine the proteins that interact with lncRNA.

The utility model relates to a kind of magnetic bead marking device, including pedestal, stand, crossbar, lifting mechanism, two-way liquid conveying mechanism, piezoelectric ceramic transducer, electromagnet and measuring cup;Stand is vertically arranged on pedestal, crossbar is movably arranged on stand by lifting mechanism;Two-way liquid conveying mechanism includes first pipeline, second pipeline, liquid suction needle and water pump, liquid suction needle is vertically arranged below the front end of crossbar, the front end of first pipeline and second pipeline is built into crossbar and is respectively communicated with the upper end of liquid suction needle, piezoelectric ceramic transducer is arranged at the lower end needle mouth of liquid suction needle;The upper end surface of pedestal is provided with placing groove, measuring cup is arranged in placing groove, the outside of measuring cup is provided with electromagnet;Through multiple modular combination, the integration of magnetic bead separation, liquid delivery, mixing function is realized, two-way liquid conveying mechanism and electromagnet work cooperatively, can complete magnetic bead adsorption, remove supernatant, flush and other steps, reduce equipment volume and operation complexity.

The utility model relates to a magnetic bead separation and purification device which comprises a mixing container, a stirring assembly and a plurality of magnets. The magnetic field of the magnet can cover the interior of the mixing container to adsorb the magnetic beads, and can not cover the interior of the mixing container to enable the magnetic beads to move freely. Thus, the stirring head extends into the mixing container to stir liquid in the mixing container, and samples can be fully mixed in cooperation with the freely moving magnetic beads; in the waste liquid discharging process of the mixing container, the magnet can be used for adsorbing the magnetic beads, so that the magnetic beads are conveniently separated when the waste liquid is discharged; in the cleaning process, the stirring head can be used for stirring to accelerate magnetic bead cleaning, the magnet can also be used for adsorbing the magnetic beads, and magnetic bead separation is facilitated when purified products are discharged.

The invention discloses a magnetic bead sorting device which comprises a sorter which is provided with a clamping plate bin, a liquid storage bin and a touch screen controller. An upper clamping edge, a lower clamping groove, a liquid injection opening, a centrifugal tube insertion hole, a first electromagnet and a second electromagnet are arranged on the clamping plate bin; a magnetic bead sorting box is clamped in the clamping plate bin, and a liquid receiving opening and a conical sorting opening are formed in the magnetic bead sorting box; a liquid injection pump is arranged on the liquid storage bin, and a magnetic field adjusting module and a pump body switch module are arranged on the touch screen controller. A magnetic bead sample purification method comprises the following steps: 1, loading a magnetic bead sorting box, starting a sorter, and filling a buffer solution; 2, uniformly mixing the magnetic bead stock solution, measuring an unseparated control group, and injecting the rest of the magnetic bead stock solution into a magnetic bead separation box; thirdly, the first electromagnet and the second electromagnet are adjusted, and magnetic beads are separated; 4, magnetic beads with medium particle sizes are selected for magnetic separation; and 5, injecting the medium-particle-size magnetic beads into the sample, and removing impurities to obtain a purified sample. The magnetic bead sorting device has the advantages that the magnetic beads with different particle sizes are easily and accurately sorted, and the sample detection precision is improved.

The invention relates to the technical field of biological detection, and particularly discloses a kanamycin colorimetric sensor based on magnetic beads and G-quadruplex aptamer / hemin, and application of the sensor in kanamycin detection in food. The sensor comprises an aptamer which can be specifically combined with kanamycin and is as shown in SEQ ID: 1 and a cDNA sequence which is complementary to the kanamycin aptamer and is as shown in SEQ ID: 2, cDNA fixed on a magnetic bead is used as a capture probe, a G-quadruplex aptamer is used as a recognition probe, chlorhematin / G-quadruplex aptamer DNA enzyme is used as a signal probe, a nano magnetic bead separation technology is combined, and the detection sensitivity is high. The qualitative and quantitative detection of kanamycin is realized. The kanamycin colorimetric sensor provided by the invention is used for detecting kanamycin in food, the operation is simple, no professional instrument is needed, the detection cost is low, and the detection efficiency is high.

The application discloses a magnetic bead continuous separation process and device, which comprises the following steps: firstly, a magnetic field is added to a magnetic bead separator; then, a raw solution is continuously added to the magnetic bead separator; under the action of the magnetic field, a magnetic bead capture area and a magnetic bead separation area are formed; the magnetic beads are captured to the capture area; impurities are discharged from a discharge port to a waste liquid tank until the concentration of the magnetic beads at the discharge port exceeds a set value; after the magnetic field is removed, a washing solution is continuously added to the magnetic bead separator, the magnetic beads are flushed out and collected to an intermediate tank; the magnetic field is applied again, the collected liquid is continuously added to the magnetic bead separator for separation again; after the magnetic field is removed, the washing solution is injected into the inlet of the magnetic bead capture area to flush out the magnetic beads until the concentration of the magnetic beads at the discharge port is lower than the set value; the above steps are recycled until the treatment is completed. The above process is automatically operated through a PLC control system. The application has the advantages of automatic feeding and discharging, completion of multiple operation steps in the same device, realization of multiple rounds of repeated separation of the magnetic beads and automatic completion.