The invention discloses a quantitative detection kit for melamine. The quantitative detection kit comprises a calibration material, a quality control material, a melamine antibody solution labeled by fluorescein isothiocyanate, a melamine antibody solution labeled by alkaline phosphatase, a sample diluent, a magnetic separation reagent, cleaning fluid and a substrate solution. According to the detection method, magnetic bead separation chemiluminescence immune detection is adopted as the detection method, and an enzyme labeling technology, a magnetic separation technology and a chemiluminescence technology are combined; operation is easy and convenient, the reaction conditions are mild, the luminescence values are stable, and the outside condition influences are few; compared with an existing detection method, the magnetic bead separation chemiluminescence immune detection method has the advantages of being simple in sample processing process, low in detection cost, rapid to detect, accurate in detection result, good in repeatability and the like.