83results about How to "Short elution time" patented technology

The regeneration process of solid acid catalyst includes making the eluant containing alkyl halide or arane halide contact with solid acid catalyst to be regenerated and subsequent separation of the eluant, and the regeneration process has the temperature of 50-200 deg.c, pressure of 0.1-4.5 MPa, liquid phase volume space velocity of the eluant 0.1-20 and elution time of 1-48 hr. The solid acid catalyst is selected from loaded heteropolyacid and its salt, loaded inorganic acid and its salt, acid oxide and zeolite molecular sieve, cationic exchange resin and solid superstrong acid. The present invention regenerates solid acid catalyst with alkyl halide as eluant, and has less consumption of eluant, short elution period and powerful regeneration capacity to deactivated catalyst.

The invention discloses a rapid and automatic determination method and device for tripolycyanamide content in dairy products, belonging to the field of food analysis. The method comprises the following steps that: in a unit (A) under an operating procedure, a first step, a multifunctional valve is in a state I, a pump A rotates, a pump B stops, a first sample flows into a column 2 for enriching tripolycyanamide, and the eluent flows into a column 1 for regenerating tripolycyanamide; a second step, the pump A stops, the pump B rotates, the cleanout fluid flows into the column 2 for cleaning the impurities therein; a third step, the multifunctional valve is switched to a state II, eluent flows into the column 2 for eluting and regenerating tripolycyanamide; the tripolycyanamide in the column 2 is substituted by the eluent and directly enters into a sampling ring of a unit (B) in the form of a sample plug, so as to perform volume quantification, injection, re-separation and detection, and a response signal is processed by a computer; and meanwhile, a second sample enters into the column 1 for enrichment, and the process of the column 2 is repeated. The method and the device are high in automation degree and excellent in reproducibility, and can analyze about 30 samples per hour; and the unit (A) in the invention can be used with various flow injection analysis systems.

The invention discloses a purification and enzyme digestion transformation method of a recombinant human insulin precursor and belongs to the field of preparation of recombinant human insulin and analogues thereof. In the invention, fermentation supernatant of a secretorily expressed recombinant human insulin precursor is purified by using a cation exchange chromatography column, two different washing solutions are used during washing of chromatography, the elution of target proteins is performed more quickly, the elution time is shortened, the precipitation of the target proteins is reduced and the volume of eluted samples is reduced; an eluting solution with pH 7.0 to 9.0 is used during elution, phase change operation does not need to be performed to eluted products, the eluted products are directly added into trypsin for enzyme digestion transformation and the transformation efficiency is high. The method disclosed by the invention is simple to operate, the primary purification and phase change operation of the fermentation supernatant are completed through one step of chromatography purification, the purity of the samples can be improved from 14 percent to 95 percent, the purified samples are directly subjected to enzyme digestion, the enzyme digestion transformation efficiency is above 95 percent, the production process is simplified and the cost is saved.

The invention provides a preparation method of a Salvia miltiorrhiza soft capsule, comprising the following steps of: completely mixing Salvia miltiorrhiza extract with glycerine and polyethylene glycol 400 so that liposoluble constituents such as tanshinone II A are dissolved in a liquid substrate so as to achieve certain solubilization and improve the biological availability of liposoluble constituents of Salvia miltiorrhiza. In the preparation process of the Salvia miltiorrhiza soft capsule, a method for preparing Salvia miltiorrhiza water-soluble component extract is provided, a macroporous resin separation technology is adopted, and ethanol solution is concentrated, atomized and dried after elution; in the obtained extract, the total salvianolic acid compounds account for more than 60% of total solid, wherein the content of salvianolic acid compound danshinolic acid B is between 45% and 60%, and the content of danshensu is controlled between 10% and 20%. The content of danshensu is controlled while the yield of salvianolic acids is improved so that the proportion of the main active ingredient danshinolic acid B is obviously improved. The preparation method is safe and efficient, low-toxic and environmentally-friendly, can satisfy the requirement of industrial production, and has wide and practical application value.

A process for extracting and separating arecaline in batches includes such steps as breaking areca, mixing with diluted hydrochloric acid, percolating, eluting with diluted hydrochloric acid, regulating pH=8-10, extracting in chloroform, concentrating, dissolving in high-concentration alcohol, filtering and recovering alcohol.

The disclosed method comprises: removing water in glucan microcolumn; adding the liposome on the column for centrifugation; measuring the total content concentration (C0) of capsaicam and the concentration of processed capsicine (C1); finally, obtaining the target value by calculating C0/C1. This invention is fast and low cost.

The invention discloses a fast detecting method for benzopyrene in edible oil. The method comprises the steps of 1, preparation of a solution for a testing product, wherein a proper amount of edible oil sample is accurately weighed and placed in a conical flask with a cover, an organic solvent is added, vibrating and shaking are carried outer after covering, ultrasonic extraction is carried out, supernate is collected after centrifugation, an organic solvent is added into the residual edible oil sample, ultrasonic extraction and centrifugation are carried out, the supernate is mixed, an organic solvent is taken and added into a solid phase extraction column for activation, the mixed supernate is transferred into the activated solid phase extraction column after activation is completed, the organic solvent is used for elution, eluant is collected into a measuring flask, volume metering is carried out, and the eluant passes through a 0.22-micrometer microfiltration membrane and enters a sample bottle for use; 2, preparation of a standard product solution; 3, detection, wherein a benzopyrene standard series solution and the solution for the testing product are sequentially injected into HPLC for detection. Two times of ultrasonic extraction and solid phase extraction column enrichment and purification are adopted, and the method is easy, convenient and safe to operate, low in cost, complete in extraction and accurate, true and reliable in detecting result.

The invention discloses a Biapenem medicine detection method and belongs to the technical field of medicine detection. The method comprises detection of Biapenem content, detection of total impurity content, detection of polymer impurities in medicine, and detection of substance A and substance B contents in medicine. The effective medicine and impurities are highly effectively separated mainly by employing liquid chromatogram technology. The detection method can be employed for highly effective, quick and accurate determination of effective substances and concrete contents of impurities in Biapenem medicine. The detection method is employed to detect quality of Biapenem medicine, and has great application value for production and quality monitoring of the Biapenem medicine.

The invention relates to a method for removing fructus trichosanthis pulp by assist of a biological enzyme, belongs to the field of medicament producing and processing, and aims at solving the technical problem of providing a method for removing fructus trichosanthis pulp by assist of a biological enzyme, and use of amylase and cellulose for removing the fructus trichosanthis pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme comprises the following steps: putting fructus trichosanthis seeds with pulp into a biological enzyme solution; taking the fructus trichosanthis seeds to wash and dry after separating the fructus trichosanthis seeds from the pulp, so as to obtain the fructus trichosanthis seeds. Furthermore, the invention also discloses use of amylase and cellulose for removing pulp. The method for removing the fructus trichosanthis pulp by assist of the biological enzyme has the advantages of being simple in process, convenient to operate, low in cost, high in removal efficiency and the like.

The invention discloses a method for detecting organic tin in a water body. The method specifically comprises the following steps: carrying out liquid-liquid extraction reflux on a to-be-detected sample solution; adding a certain amount of extract liquor into a phosphate citrate buffer solution, normal hexane and a sodium tetraethylborate solution for derivatization; separating the extract liquorthrough a chromatographic column, and comparing with a standard solution by using a flame photometric method to obtain a standard curve, so as to calculate and detect the organic tin in the water body. According to the method, a liquid-liquid extraction gas chromatography flame photometry detector is adopted to determine the concentration of the organic tin compound in the water, derivatization and extraction are performed at the same time in the step S4; therefore, compared with solid-phase extraction in the background art, complex elution steps are not needed, the elution time is saved, theextraction and derivatization time is shortened, operation is easy and rapid. In addition, the actual sample analysis requirements can be met.

The invention relates to a method of preparing spheroid polymer particles having a narrow size distribution by dispersion polymerization. This method comprises the steps of: providing a two-phase system in the form of a dispersion comprising an organic phase of droplets dispersed in an aqueous medium, mixing said organic phase in said aqueous medium under agitation without using a dispersion stabilizing agent to stabilize the dispersion, wherein the organic phase comprises at least a crosslinkable monomer, a polymerization initiator and an organic solvent for said monomer, and allowing the crosslinkable monomers to polymerize, while the two-phase system is agitated.

The present invention discloses a preparation method of a porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent. According to the preparation method, waste ceramic is used as the supporting body of an adsorbent, and perfluorooctane sulfonic acid is used as a template molecule; 58-65% by mass of N,N-dimethylformamide, 15-20% by mass of succinimide, 1.0-3.0% by mass of perfluorooctane sulfonic acid, 12-18% by mass of aminated porous ceramic powder, and 1.0-4.0% by mass of ammonium persulfate are added to a reactor, a stirring reaction is performed for 6 h at a temperature of 75+/-2 DEG C in the absence of oxygen, the obtained product is subjected to stirring washing for 12 h with the mixed solution of ethanol and sodium hydroxide, the template molecule is removed, and drying is performed to obtain the porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent. According to the present invention, the porous ceramic surface perfluorooctane sulfonic acid molecularly imprinted adsorbent can specifically recognize perfluorooctane sulfonic acid, and has advantages of high selectivity, good mechanical property, good chemical stability, fast adsorption, easy elution, and regenerative capacity.

The invention provides a method for determining total fluorine compound precursor substances in an atmospheric particulate matter sampling filter membrane; the method comprises the following steps of1) extracting a sample: carrying out ultrasonic extraction on the atmospheric particulate matter sampling filter membrane, and collecting to obtain an extracting solution; 2) purification of the sample: carrying out purifying, concentrating and purifying on the extracting solution obtained in the step 1) to obtain a sample detection solution; and 3) determination of the sample: detecting the sample detection solution obtained in the step 2) by adopting an ultra-high performance liquid phase chromatography-triple quadrupole series mass spectrometer, and obtaining the content of the total fluorine compound precursor substance by adopting an internal standard method. The invention belongs to the technical field of environmental monitoring, and by virtue of the provided measurement method, full extraction and measurement of six kinds of total fluorine compound precursor substances, such as 4:2FTS and the like in the atmospheric particulate matter sampling filter membrane can be realized, the separation effect is good, and the detection efficiency is improved; and the method has the advantages of being accurate in measurement result, high in repeatability, high in sensitivity, high in recovery rate, low in detection limit, high in substrate interference resistance and the like.

The invention discloses a measuring method of structural isomers of perfluorochemicals in an atmospheric particulate sampling membrane, and belongs to the technical field of environmental monitoring.The measuring method comprises the following steps of 1, sample extracting, wherein the atmospheric particulate sampling membrane is subjected to ultrasonic extraction, and thus an extraction solutionis collected and obtained; 2, sample purifying, wherein the extraction solution obtained in step 1 is purified, concentrated and repurified, and thus a sample detection solution is obtained; 3, sample measuring, wherein the sample detection solution obtained in step 2 is detected by an ultra-performance liquid chromatography-triple quadrupole tandem mass spectrometer, quantitative calculation isconducted through an internal standard method, and thus the content of the structural isomers of the perfluorochemicals in the atmospheric particulate sampling membrane is obtained. By the adoption ofthe measuring method, the structural isomers of the perfluorochemicals in the atmospheric particulate sampling membrane are sufficiently extracted and measured.

The invention provides a purifying method for a high-purity echinocandin B mother nucleus or a salt thereof. The purifying method comprises the following steps: dissolving a crude product of an echinocandin B mother nucleus or a salt thereof to prepare a sample and loading the sample into a preparative high-performance liquid chromatographic column; carrying out elution with mixed liquor of an aqueous solution and an organic solvent as a mobile phase; and fractionally collecting eluates. The purifying method is simple to operate, high in purifying efficiency and easy in process flow; the recovery rate of the high-purity echinocandin B mother nucleus or the salt thereof is more than 85%; a small amount of organic solvents are used, so the method is friendly to environment; the method has good process reproducibility, can realize mass product, is high and stable in recovery rate and saves cost; product purity reaches 99% or above; the process of separation and purification is simple; andthe concentration of an organic phase is low, so elution time is short, and cost is saved.

The invention discloses a method for efficiently and accurately measuring glycidyl esters in vegetable oil. The method comprises the following steps of (1) the pretreatment of a sample to be measuredincluding the steps of dissolving a vegetable oil sample to be measured by using a mixed solvent consisting of methyl tert-butyl ether and ethyl acetate, feeding the dissolved vegetable oil sample tobe measured into a solid phase extraction cartridge, eluting, blowing nitrogen and redissolving to obtain a solution to be measured, and (2) UPLC-MS/MS determination including the steps of carrying out liquid chromatography and mass spectrometry determination on the solution to be measured to obtain the content of the glycidyl esters (GEs). The detection method provided by the invention has strongoperability, good sensitivity and accurate and good reproducibility, compared with the prior art, the accurate and quantitative detection of the GEs in an actual edible vegetable oil sample can be met and the method is more suitable for application of university researchers and industrialization.