The invention discloses a method for detecting and evaluating conjunctival irritation and mucous membrane irritation by using chicken embryo vascular system, comprising the following steps: (1) preparation of chorioallantoic membrane; (2) conjunctival irritation test; (3) sticking mold Stimulation test; (4) prediction model and classification, the present invention uses the standardized chicken embryo as the test system, and its vascular system at different stages of development is similar to the human eye conjunctiva and oral submucosa tissue, and the chicken embryo has a high degree of standardization, stable source and batch The difference between blood vessels is small, the vascular system has biological activity and function, the test system is simple and cheap, no special equipment is required, and the detection method is fast, sensitive and universal. The invention can detect and evaluate the conjunctival irritation and mucous membrane irritation of the substance to be tested from both qualitative and quantitative aspects. The method of the present invention can also replace living animals and human volunteers, and be directly used for the detection of conjunctival irritation and mucosal irritation of substances such as chemicals or cosmetics, and rapid screening, classification and identification thereof.