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Method for extracting 6-hydroxy kaempferol monomer compound from marigold

A compound, the technology of marigold, is applied in the field of extracting 6-hydroxykaempferol monomer compound from marigold, which can solve the difficulty of ensuring clinical drug effectiveness, safety, sustainability, lack of drug effect material basis and drug effect Research, lack of quality control standards and other issues, to achieve the effect of shortening the preparation time, broadening the source of raw materials, and reducing the column elution time

Active Publication Date: 2017-12-19
TIANJIN YAOYU BIOLOGICAL TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the ingredients of current marigold preparations are generally mixtures. For example, the whole marigold flower is air-dried and used as medicine, which lacks the necessary material basis and efficacy research, so it lacks reasonable quality control standards, and it is difficult to ensure the effectiveness of clinical medication. safety, sustainability

Method used

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  • Method for extracting 6-hydroxy kaempferol monomer compound from marigold
  • Method for extracting 6-hydroxy kaempferol monomer compound from marigold
  • Method for extracting 6-hydroxy kaempferol monomer compound from marigold

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Embodiment 1

[0034] The present embodiment extracts the method for 6-hydroxy kaempferol monomer compound from marigold, comprises the following steps:

[0035] Take dried marigold flowers as raw materials, grind them into powder, extract and rotary steam with ethanol to obtain dried marigold coarse powder, weigh 5g, dissolve in 50mL of 95% methanol-water solution, and prepare a marigold extract solution with a concentration of 100mg / mL, passed through a 0.45 μm microporous membrane, and prepared by one-dimensional liquid chromatography. One-dimensional liquid chromatography using reversed-phase column DAISO C 18 (250mm×20mmi.d., 8μm), the mobile phase is a mixture of organic phase and water phase, wherein phase A is water, phase B is methanol, isocratic elution method: concentration of phase B is 53% isocratic for 20min. Using UV detector 210nm and 260nm as dual selective absorption wavelengths, preparation temperature is room temperature, injection volume is 500μL / needle, mobile phase f...

Embodiment 2

[0039] The present embodiment extracts the method for 6-hydroxy kaempferol monomer compound from marigold, comprises the following steps:

[0040] Take dried marigold flowers as raw materials, grind them into powder, extract and rotary steam with ethanol to obtain dried marigold coarse powder, weigh 5g, dissolve it in 100mL 95% methanol-water solution, and prepare a marigold extract solution with a concentration of 25mg / mL, passed through a 0.45 μm microporous membrane, and prepared by one-dimensional liquid chromatography. One-dimensional liquid chromatography using reversed-phase column DAISO C 18 (250mm×20mmi.d., 8μm), the mobile phase is a mixture of organic phase and water phase, wherein A phase is water, B phase is methanol, isocratic elution method: B phase concentration 55% isocratic 20min. Adopting ultraviolet detector 210nm and 260nm as dual selective absorption wavelengths, preparation temperature is room temperature, injection volume is 500 μ L / needle, mobile pha...

Embodiment 3

[0043] The present embodiment extracts the method for 6-hydroxy kaempferol monomer compound from marigold, comprises the following steps:

[0044] Take dried marigold flowers as raw materials, grind them into powder, extract and rotary steam with ethanol to obtain dried marigold coarse powder, weigh 5g, dissolve in 25mL 95% methanol-water solution, and prepare marigold extract solution with a concentration of 200mg / mL, passed through a 0.45 μm microporous membrane, and prepared by one-dimensional liquid chromatography. One-dimensional liquid chromatography using reversed-phase column DAISO C 18 (250mm×20mmi.d., 8μm), the mobile phase is a mixture of organic phase and water phase, wherein A phase is water, B phase is methanol, isocratic elution method: B phase concentration 50% isocratic 20min. Adopting ultraviolet detector 210nm and 260nm as dual selective absorption wavelengths, preparation temperature is room temperature, injection volume is 500 μ L / needle, mobile phase fl...

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Abstract

The invention provides a method for extraction of a 6-hydroxy kaempferol monomer compound from Tagetes erecta. The method includes: taking Tagetes erecta dried flower, grinding the Tagetes erecta into powder, then subjecting the powder to ethanol soaking or reflux extraction, evaporating the filtrate to dryness, and then dissolving the extract in a methanol-water solvent; conducting one-dimensional liquid chromatography separation: employing a DAISO C18 chromatographic column and a water-methanol binary mobile phase, conducting isocratic elution with phase B at a concentration of 50-55%, collecting the 13-18min eluent, performing evaporation concentration to dryness; then dissolving the component in a methanol-water solvent; conducting two-dimensional liquid chromatography separation: employing an Acchrom X-Amide chromatographic column and a water-acetonitrile binary mobile phase, conducting isocratic elution with phase B at a concentration of 80-90%, collecting the 8-10min eluent, and carrying out evaporation concentration to dryness so as to obtain a target product. The method provided by the invention separates 6-hydroxy kaempferol from Tagetes erecta for the first time, and can reach purity up to more than 98%, thus providing more accurate guiding basis for quality control of Tagetes erecta and its preparations and rational clinical use of drugs.

Description

technical field [0001] The invention relates to the technical field of extraction of effective plant components, in particular to a method for extracting 6-hydroxykaempferol monomer compounds from marigolds. Background technique [0002] Marigold, also known as golden chrysanthemum, scientific name Tagetes erecta, belongs to Asteraceae Tagetes, is an annual herb plant, in addition to having ornamental value, it also has good medicinal value, such as its root has detoxification and detumescence effect, used for treatment Respiratory tract infection, whooping cough, bronchitis, stomatitis, mastitis, etc., its leaves are used for carbuncles, sores, furuncles, furuncles, and unknown swollen toxins, and its flowers are used to calm the liver, relieve heat, dispel wind and resolve phlegm, and are used to treat dizziness and head wind Eye pain, convulsions in children, cold and cough, breast pain and other symptoms. However, the ingredients of current marigold preparations are gen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07D311/30C07D311/40
CPCC07D311/30C07D311/40
Inventor 张耀洲陆洪韩朝杨珊珊马红庞莉王欢欢李上文盖其静杜思邈
Owner TIANJIN YAOYU BIOLOGICAL TECH
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