73 results about "Uv detector" patented technology

The invention relates to the technical field of drug analysis and detection, and provides a detection method of moxifloxacin hydrochloride related substances. The related substances of moxifloxacin hydrochloride are detected by adopting a high performance liquid chromatography method, and the detection conditions are as follows: octadecylsilane chemically bonded silica is taken as a filling agent of a chromatographic column; the wavelength of a UV detector is 296 nm; the column temperature is 35-45 DEG C; the flow velocity of the mobile phase is 1.6-1.8 ml / min; the sample injection volume is 10-50 [mu] l; the mobile phase A is phosphate buffer solution-acetonitrile-methanol; the mobile phase B is phosphate buffer solution-acetonitrile-methanol; the elution mode is gradient elution; the diluent is a mobile phase A; according to the method provided by the invention, effective separation between moxifloxacin hydrochloride and various impurities can be realized, the sensitivity is high, the accuracy is good, the reproducibility is relatively good, a good linear relation is shown in a certain range, and qualitative and quantitative determination can be carried out on various impurities in moxifloxacin hydrochloride bulk drugs.

Provided is a curing agent for epoxy resins which comprises an aromatic amine compound (A-1) and one or more aromatic amine adducts (A-2) that are products of reaction between an aromatic amine compound and a reactive compound having a functional group capable of reacting with the aromatic amine compound, wherein the aromatic amine adducts (A-2) include an aromatic amine adduct (A-2-1) formed by uniting one molecule of the reactive compound to one molecule of the aromatic amine compound (A-1) by addition reaction. The ratio of the aromatic amine compound to the aromatic amine adduct (A-2-1), (A-1) / (A-2-1), obtained by analysis by liquid chromatography (UV detector), is 0.50-2.00.

The invention belongs to the field of cosmetics, and particularly relates to a blue-copper peptide related substance detection method which adopts a high performance liquid chromatograph for detection, and the chromatographic conditions are as follows: a chromatographic column is a reversed-phase C18 or C8 reversed-phase chromatographic column; a mobile phase: phosphate; the wavelength of an ultraviolet detector is 220nm; the column temperature is 25-40 DEG C; the flow velocity of the mobile phase is 0.5-1.0 ml / min; the elution procedure is equal-gradient elution. The chromatographic column in the detection method of the blue-copper peptide related substance can realize separation of the ligand compound and the blue-copper peptide, the separation reaches 1.5, and qualitative and quantitative accurate detection of the blue-copper peptide related substance can be realized based on the excellent separation effect of the chromatographic column.

The invention relates to the technical field of polypeptide detection, and particularly discloses a method for determining the content of human epidermal growth factors in sodium hyaluronate composite gel. The method comprises the following steps: S1, carrying out enzymolysis on sodium hyaluronate in the sodium hyaluronate composite gel by using an enzyme to obtain a test solution; s2, measuring a blank matrix solution containing no human epidermal growth factors, and adding enzyme for enzymolysis to obtain a blank matrix solution; s3, measuring the human epidermal growth factor, and diluting the human epidermal growth factor with the blank matrix solution to obtain a reference substance solution; and S4, detecting the test solution and the reference solution by using a high performance liquid chromatography and an ultraviolet detector, and determining the content of the human epidermal growth factor by using an external standard method. According to the method, hyaluronidase is used for pre-treating the test sample, macromolecular and high-viscosity sodium hyaluronate is subjected to enzymolysis to form micromolecular sugar, the method is compatible with the same liquid chromatography separation system, the content of the human epidermal growth factors in the test sample is measured by adopting an HPLC (High Performance Liquid Chromatography) combined ultraviolet detector, and the method is high in specificity, high in accuracy, low in detection limit and good in repeatability.

The invention belongs to the technical field of medicine analysis, and particularly relates to a method for detecting an impurity A, an impurity B, an impurity C and an impurity D. High performance liquid chromatography is adopted for detection, and chromatographic conditions are as follows: a Titank Phenyl-Hexyl chromatographic column is adopted as a chromatographic column; acetonitrile and a buffer solution with the ratio of 4: 96 v / v are used as a mobile phase for isocratic elution, and every 1 L of the buffer solution contains 8.00 g of sodium 1-octanesulfonate, 37.00 mg of EDTA-disodium, 10 mL of perchloric acid and 2 mL of phosphoric acid; the flow velocity of the mobile phase is 0.6-1.0 mL / min, the column temperature is 15-25 DEG C, the sample size is 5-15 mL, an ultraviolet detector is used for detection, and the detection wavelength is 210-220 nm; the method can detect impurities A, B, C and D in the zoledronic acid bulk drug, and can be used for quality control of the zoledronic acid bulk drug.

The invention discloses a method for detecting an N-nitroso compound in vortioxetine hydrobromide, and the N-nitroso compound is 2, 2 '-(nitroso amino) bis-acetonitrile. According to the method, a high performance liquid chromatography is adopted, an octadecyl silane bonded silica gel filler chromatographic column and an ultraviolet detector are adopted, a mobile phase A is 0.05% phosphoric acid aqueous solution, a mobile phase B is methanol, and gradient elution is adopted. The method has good specificity and sensitivity.

The invention relates to the technical field of analysis and detection, and discloses a construction method of a fingerprint spectrum of relevant characteristic peaks of rhizoma coptidis paste and radix rehmanniae recen, and the construction method comprises the following steps: S1, preparing a reference solution and a test solution; and S2, injecting the reference substance solution and the test solution into a high performance liquid chromatograph, adopting a chromatographic column with octadecylsilane chemically bonded silica as a filler, taking acetonitrile as a mobile phase A, taking a 0.0125 mol / L monopotassium phosphate solution as a mobile phase B, eluting according to a specified gradient program, and detecting by adopting an ultraviolet detector to obtain the fingerprint spectrum. By means of the provided construction method of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen, the sequence and the mutual relation of the characteristic peaks can be indicated, and the effect of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen is effectively represented.

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

The utility model relates to a large-scale pharmaceutical production level nucleic acid synthesizer which comprises a monomer pipeline unit, a reagent pipeline unit and a solvent pipeline unit, each single pipeline unit comprises a first inlet valve group, a first flow cell, a first flow meter, a single pump, a first switching valve, a second switching valve, a second flow meter, a second flow cell, a column position valve and an outlet valve which are communicated in sequence, a first UV detector and a second UV detector are arranged at the two ends of the column position valve, and a third flow cell is arranged between the second UV detector and the outlet valve; the reagent pipeline unit comprises a second inlet valve group, a reagent pump, a fourth flow cell, a third flow meter and a T-shaped valve which are communicated in sequence, and the T-shaped valve is communicated with the first switching valve; the solvent pipeline comprises a third inlet valve group, a circulating pump and a fifth flow cell, and the output end of the fifth flow cell is communicated with the second switching valve. The device is simple in overall structure, small in size and short in synthesis pipeline, and the reaction speed and the detection efficiency of each detector are improved.

A photodetector and method of making the same including a semiconductor and a 2D material. The space charge region is located directly at the surface of the semiconductor. A photon with a small wavelength, once it enters the semiconductor, is immediately inside the space charge region in which an electron-hole pair generated by the photon can contribute to a photocurrent. This makes the photodetector an efficient UV detector. Interdigitated oxide structures between the semiconductor and the 2D material further enhance the sensitivity of the photodetector to, for example, UV light.

The present invention relates to a method for identifying buckwheat honey, the method comprising: (1) extracting norleucine (Nle) in a honey sample and adding phenyl isothiocyanate for derivatization reaction; (2) using a high performance liquid chromatography-ultraviolet detector to quantitatively detect norleucine derivatives in the honey sample; (3) performing comparative analysis on the norleucine content in the honey sample, and determining the type of the sample to be tested according to the norleucine content; when norleucine is detected in the honey sample to be tested, it is determined that the sample to be tested contains buckwheat honey.

This invention relates to the field of pharmaceutical analysis technology, specifically to a method for determining the content of deoxycholic acid injection. The detection method employs high-performance liquid chromatography (HPLC) with an ultraviolet (UV) detector. The HPLC chromatographic conditions are as follows: the column is an octadecylsilane-bonded silica column; the column dimensions are 4.6 × 150 mm, and the packing diameter is 3 μm; the detection wavelength is 220 nm; the mobile phase is 0.1% formic acid aqueous solution – 0.1% formic acid acetonitrile solution (42:58). Compared with existing technologies, UV detectors are more readily available than CAD detectors. This invention achieves quantitative detection of deoxycholic acid injection content by controlling the HPLC conditions of the UV detector. This method exhibits baseline stability, good specificity, accuracy, and precision, and is of significant importance for the quality control of deoxycholic acid injection.

Provided is an epoxy resin which contains a compound X represented by formula (1), and which has a content ratio of an oligomer comprising the compound X of 0.01-19.0 area% when the total area of detected peaks is taken as 100 area% in a reversed-phase HPLC measurement using a 280 nm UV detector. In formula (1), the plurality of R (s) may be the same as or different from each other, are hydrogen atoms or hydrocarbon groups, and may contain heteroatoms, the plurality of R (s) may be bonded to each other to form a ring, and at least one of the plurality of R (s) is a hydrocarbon group. # imgabs0 #

This invention belongs to the field of traditional Chinese medicine preparation detection technology, specifically relating to a method for constructing a fingerprint spectrum of Shuangling Zhixie granules and performing quality detection. Utilizing ultra-high performance liquid chromatography (UHPLC), specifically, it includes using octadecylsilane-bonded silica gel as the column packing material, acetonitrile as mobile phase A, and phosphoric acid solution as mobile phase B, performing gradient elution, and using a UV detector to detect and obtain chromatograms. The chromatograms are then processed using fingerprinting software to obtain fingerprint spectra of multiple batches of Shuangling Zhixie granules. The fingerprint spectra of Shuangling Zhixie granules are then imported into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, and after multi-point correction, a control fingerprint spectrum of Shuangling Zhixie granules is generated using the average method. The fingerprint spectrum of the Shuangling Zhixie granule sample to be tested is compared with the control fingerprint spectrum of Shuangling Zhixie granules, and the similarity is calculated. This method can more comprehensively and effectively evaluate and control the quality of Shuangling Zhixie granules; the method is simple, stable, and has good repeatability.

This invention relates to the field of pharmaceutical analysis and detection technology, and proposes a method for detecting related substances in methylcobalamin tablets. The method employs reversed-phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet (UV) detector. The chromatographic conditions of the RHPLC-UV detector method include: mobile phases A and B; mobile phase A is a 92:8 mixture of potassium dihydrogen phosphate solution and acetonitrile, and mobile phase B is a 92:8 mixture of acetonitrile and water; gradient elution is used. This technical solution solves the problem of inaccurate detection of related substances in methylcobalamin tablets in related technologies.

The utility model relates to the technical field of recombinant protein chromatography detection, in particular to recombinant protein batch chromatography detection equipment. A batch chromatography detection mechanism composed of a material tank, a material injection pump, a batch conveying pipe frame, a supporting plate, a sealing cover, a chromatographic column, a UV detector and a collecting tank is jointly mounted among a placement seat, a cross-shaped supporting plate frame I, a cross-shaped supporting plate frame II and a support; according to the present invention, the three chromatographic columns are arranged, such that the equipment can simultaneously perform separation detection on the recombinant protein in the three chromatographic columns during the one-time separation and purification process so as to effectively reduce the separation and purification time of the batch recombinant protein; the large-batch conveying pipe frame is communicated with the chromatographic columns on the different branch plates on the second cross-shaped supporting plate frame, it can be guaranteed that one set of chromatographic columns can be continuously used when the other set of chromatographic columns are blocked, and therefore the continuity of the large-batch recombinant protein separation and purification process is guaranteed, and the large-batch recombinant protein separation and purification efficiency is higher.

The invention provides a method for separating and determining related substances of a remegapam intermediate by using HPLC (High Performance Liquid Chromatography), which comprises the following steps: carrying out gradient elution by using octadecylsilane chemically bonded silica as a chromatographic column filler, 0.01 mol / L potassium hexafluorophosphate solution as a mobile phase A and a solution obtained by uniformly mixing methanol and acetonitrile according to a volume ratio of (55-65): (35-45) as a mobile phase B at the flow velocity of 0.8-1.2 ml / min and the column temperature of 25-40 DEG C, the method comprises the following steps: detecting related substances of the remegapam intermediate by adopting an ultraviolet detector, wherein the detection wavelength of the ultraviolet detector is 260-270nm; the related substances of the remegapam intermediate are one or more of an impurity I, an impurity II and an impurity III. According to the present invention, the impurity I, the impurity II and the impurity III in the remegapam intermediate can be rapidly and efficiently separated under the same chromatographic condition so as to effectively control the quality of the bulk drug, and the detection method has advantages of rapidness, simpleness, convenience, high sensitivity, strong specificity, high precision, accuracy, reliability and convenient operation.

The present invention discloses a high performance liquid chromatography method for detecting free chiral amino acid fingerprints of protein products and its application. First, free amino acids are extracted from protein products, and pre-column derivatization treatment is performed using Nα-(2,4-dinitro-5-fluorophenyl)-L-aminopropionamide, and separation and detection are performed using a high performance liquid chromatography-ultraviolet detector equipped with an Acclaim C18 chromatographic column, and finally the content of each amino acid is calculated using a standard curve. In this method, the derivative product has good stability within 15 hours; the analysis time for a single sample is 75 minutes; it makes up for the defects of the current high equipment cost of such methods, low stability of derivatized products, and long detection time for a single sample; it realizes the qualitative and quantitative characterization of the free chiral amino acid fingerprints of protein products, and provides key information for research on the nutritional metabolism and physiological functions of food-borne D-amino acids.

The present application relates to a food detection method, and particularly to a method for determining calcium beta-hydroxy-beta-methylbutyrate in high-protein food. 18 The sample is extracted by methanol water ultrasonic extraction, purified by PXC solid phase extraction column, separated by HPLC, eluted by acetonitrile-0.02 mol / L potassium dihydrogen phosphate solution as the mobile phase at a flow rate of 1.0 mL / min, detected by a UV detector, and quantified by an external standard method. The mass concentration of calcium beta-hydroxy-beta-methylbutyrate in the range of 0.020-2.00 mg / mL has a good linear relationship with the peak area, the correlation coefficient is 0.9993, the quantitative limit of special medical purpose formula food is 0.4 g / 100g, and the detection limit is 0.2 g / 100g; the quantitative limit of sports nutrition food is 1.0 g / 100g, and the detection limit is 0.5 g / 100g. The standard addition recovery is between 92.9% and 104%, and the relative standard deviation is 0.94%-3.93%. The method has simple pretreatment, good purification effect and high specificity, and can meet the content determination of calcium beta-hydroxy-beta-methylbutyrate in special medical purpose formula food and sports nutrition food.

The invention relates to a quantitative analysis method for intermediates in the synthesis process of brassinolide compounds. The analysis method is a gas chromatography method, a sterol series product is used as an internal standard, an SH-Rtx-5 (30m*0.25mm*0.1um) capillary column and an FID detector are used, and a heating program is as follows: a starting temperature of 150-200°C is maintained for 1-5min, and then the temperature is increased to 230-300°C at a heating rate of 10°C / min-20°C / min, and the temperature is maintained for 10min-20min. The invention conducts qualitative and quantitative analysis on ergosteroid-2,22-diene-6-one, an intermediate in the synthesis process of brassinolide, and solves the problems that ergosteroid-2,22-diene-6-one has low sensitivity to ultraviolet detectors and DAD detectors, and that evaporative light detectors have poor reproducibility in quantitative detection.

A photodetector and method of making the same including a semiconductor and a 2D material. The space charge region is located directly at the surface of the semiconductor. A photon with a small wavelength, once it enters the semiconductor, is immediately inside the space charge region in which an electron-hole pair generated by the photon can contribute to a photocurrent. This makes the photodetector an efficient UV detector. Interdigitated oxide structures between the semiconductor and the 2D material further enhance the sensitivity of the photodetector to, for example, UV light.

The invention provides a method for detecting related components in dipalmitoyl phosphatidylcholine and application of the method, and relates to the technical field of substance analysis. Wherein the related components comprise one or more of palmitic acid, 1-palmitoyl-sn-glycerol-3-phosphorylcholine, glycerophosphorylcholine and imidazole. The invention further discloses a preparation method of the pharmaceutical composition. According to the detection method, related components are separated by adopting a high performance liquid chromatography, a chromatographic column is a hydrophilic interaction chromatographic column, n-hexane and isopropanol form a mobile phase A, methanol is taken as a mobile phase B, and the related components are separated and determined by adopting gradient elution and an evaporative light-scattering detector connected in series with an ultraviolet detector. And obtaining a chromatogram and calculating the content of related components by using an external standard method. The detection method has the characteristics of strong specificity, good separation degree, good precision, high accuracy, simplicity, convenience and rapidness in operation and the like, and can be effectively used for controlling related substances of process intermediates and final products of a dipalmitoyl phosphatidylcholine production process.

The present invention provides a dissolution and detection method for long peptides, which mainly solves the technical problem that the polypeptide samples have poor sample solubility due to a large number of amino acid compositions (more than 50AA) or a large number of hydrophobic amino acids in the sequence, resulting in abnormal test results. The present invention first dissolves the sample with trifluoroacetic acid and acetonitrile at appropriate concentrations, and then injects it into a high performance liquid chromatography analyzer, using C3 bonded silica gel as a chromatographic column filler, using an ultraviolet detector, selecting acetonitrile and water as mobile phases, trifluoroacetic acid as an ion pair reagent, and gradient elution; the detection wavelength is 220 nm; the flow rate is 0.4 ml / min; the injection volume is 1 ul; and the column temperature is 35 ° C. This method effectively solves the detection problem caused by the difficulty in dissolving long peptides and improves the quality control of samples.

The invention discloses an analysis method for precolumn derivatization of a hydrogenation impurity of a melogabalin besylate intermediate, the melogabalin besylate intermediate is MB-2, and the hydrogenation impurity in the melogabalin besylate intermediate is MB-2A, the analysis method comprises the following steps: (1) using a derivatization reagent to perform precolumn derivatization on the melogabalin besylate intermediate; carrying out derivative reaction on the melogabalin besylate intermediate under the catalysis of alkali to obtain derivative reaction liquid; the derivatization reagent is o-phthalaldehyde, fluorenyl methyl chloroformate or 2, 4-dinitrofluorobenzene; and (2) detecting the derivative reaction liquid by using a high performance liquid chromatography-UV detector to obtain the content of the hydrogenated impurities in the melogabalin besylate intermediate. According to the analysis method, the content of MB-2A in MB-2 can be effectively separated and accurately quantified, the accuracy is high, the detection line is low, and the method is high in durability. # imgabs0 #

This invention relates to the field of pharmaceutical analysis technology, specifically to a method for determining the content of L-2-aminobutyric acid (GABA), a degradation impurity, in a concentrated solution of levetiracetam for injection. The method includes the following steps: diluting the concentrated levetiracetam for injection with a 50% acetonitrile aqueous solution, and then determining the content using high-performance liquid chromatography (HPLC) with a UV detector. C0.05 is also used. 18 The chromatographic column uses an aqueous solution of ammonium dihydrogen phosphate and acetonitrile as the mobile phase. This invention provides an accurate method for determining the degradation impurity L-2-aminobutyric acid in levetiracetam, which is highly specific, sensitive, accurate, and easy to operate, effectively controlling the quality of concentrated levetiracetam injection solutions.

The invention discloses a method for analyzing and determining 4-nonylphenol, bisphenol A and pentachlorophenol in water, which comprises the following steps of: eluting impurities in a solid-phase extraction column by using a reagent, adding a water sample into the solid-phase extraction column, leaving a compound to be detected in a filler of the solid-phase extraction column, discarding water, eluting the compound to be detected in the filler by using an organic reagent, and detecting the content of 4-nonylphenol, bisphenol A and pentachlorophenol in the solid-phase extraction column. The method comprises the following steps of: adding 4-nonylphenol and bisphenol A into an organic solvent, concentrating, separating through liquid chromatography, detecting pentachlorophenol through an ultraviolet detector, detecting 4-nonylphenol and bisphenol A through a fluorescence detector, and determining nature and quantifying peak area through retention time of an ultraviolet spectrogram and a fluorescence spectrogram. The method overcomes the defect that three traditional compounds need to be independently analyzed and determined, is simple and convenient to operate and easy to implement, does not need expensive materials, can be used for obtaining reagent consumables in situ, saves the manpower and material resource cost, and improves the efficiency of analysis and determination operation.

The invention discloses a method for determining aldehyde compounds in epoxypropane, which belongs to the field of liquid chromatography detection, and is characterized by comprising the following four steps: step 1, equipment selection: a high performance liquid chromatograph is provided with a UV detector, step 2, standard curve drawing: (formaldehyde, acetaldehyde and propionaldehyde), step 3, formaldehyde, acetaldehyde and propionaldehyde content determination in a sample, and step 4, aldehyde compound content determination. And 4, taking the to-be-detected solution and the blank solution epoxypropane as important chemical raw materials, solving the problems of low response, incomplete analysis types and low formaldehyde gas chromatography accuracy of the existing gas chromatography for detecting aldehydes, and being mainly applied to the aspect of detecting aldehyde compounds in epoxypropane.

The invention mainly relates to the technical field of drug analysis and detection, in particular to an impurity detection method of a compound amino acid injection. High performance liquid chromatography is adopted for detection, and chromatographic conditions are as follows: a chromatographic column adopts octadecylsilane chemically bonded silica as a filler; the wavelength of a UV detector is 210 to 225 nm; mobile phases: a mobile phase A: a sodium dihydrogen phosphate solution with a pH value of 4.0-6.5 and methanol; a mobile phase B: acetonitrile and water; the elution mode is gradient elution; the impurities comprise a tryptophan impurity B, a tryptophan impurity C, a tryptophan impurity D, a tryptophan impurity E, a tryptophan impurity H, a tryptophan impurity G and indole-3-formaldehyde. The detection method disclosed by the invention is good in specificity and high in accuracy; different detection methods do not need to be adopted for separate detection, multiple impurities in the preparation can be detected at the same time, and the detection frequency is reduced; the detection time is shortened and the detection cost is saved; a reliable method is provided for the preparation process of the compound amino acid injection and the quality control of the product, and the medication safety of a patient is improved.

The application discloses a detection method of indole-3-methanal in compound amino acid injection, and belongs to the field of analytical chemistry. The detection method adopts high performance liquid chromatography, and the chromatographic conditions include: a chromatographic column with octadecylsilane bonded silica as a filler, isocratic elution with a mobile phase, detection by a UV detector with a detection wavelength of 301-311 nm, a flow rate of the mobile phase of 0.6-1.0 mL / min, a sample injection amount of 50-150 muL, a column temperature of 30-40 DEG C, and a mobile phase of a mixed solution of acetonitrile and phosphate buffer solution. The application selects the proportion of the mobile phase, pH, flow rate, column temperature, wavelength and sample injection amount and other chromatographic conditions in the light of the low response and small polarity of the impurity by using high performance liquid chromatography, and the method is verified systematically, and has the advantages of strong specificity, high sensitivity, good precision, strong durability and the like, and can qualitatively or quantitatively detect indole-3-methanal in the compound amino acid injection, so that the effectiveness and safety of the related products are improved.

A curing agent for epoxy resins, which contains an aromatic amine compound (A-1) and an aromatic amine adduct (A-2) that is a reaction product of an aromatic amine compound and a reactive compound having a functional group capable of reacting with the aromatic amine compound, the aromatic amine adduct (A-2) contains an aromatic amine adduct (A-2-1) obtained by adding one molecule of a reactive compound to one molecule of an aromatic amine compound (A-1), and the ratio (A-1 / A-2-1) of the aromatic amine compound to the aromatic amine adduct (A-2-1) obtained by liquid chromatography (UV detector) is 0.50-2.00.