27 results about "Uv detector" patented technology

The invention relates to the technical field of polypeptide detection, and particularly discloses a method for determining the content of human epidermal growth factors in sodium hyaluronate composite gel. The method comprises the following steps: S1, carrying out enzymolysis on sodium hyaluronate in the sodium hyaluronate composite gel by using an enzyme to obtain a test solution; s2, measuring a blank matrix solution containing no human epidermal growth factors, and adding enzyme for enzymolysis to obtain a blank matrix solution; s3, measuring the human epidermal growth factor, and diluting the human epidermal growth factor with the blank matrix solution to obtain a reference substance solution; and S4, detecting the test solution and the reference solution by using a high performance liquid chromatography and an ultraviolet detector, and determining the content of the human epidermal growth factor by using an external standard method. According to the method, hyaluronidase is used for pre-treating the test sample, macromolecular and high-viscosity sodium hyaluronate is subjected to enzymolysis to form micromolecular sugar, the method is compatible with the same liquid chromatography separation system, the content of the human epidermal growth factors in the test sample is measured by adopting an HPLC (High Performance Liquid Chromatography) combined ultraviolet detector, and the method is high in specificity, high in accuracy, low in detection limit and good in repeatability.

The invention relates to the technical field of analysis and detection, and discloses a construction method of a fingerprint spectrum of relevant characteristic peaks of rhizoma coptidis paste and radix rehmanniae recen, and the construction method comprises the following steps: S1, preparing a reference solution and a test solution; and S2, injecting the reference substance solution and the test solution into a high performance liquid chromatograph, adopting a chromatographic column with octadecylsilane chemically bonded silica as a filler, taking acetonitrile as a mobile phase A, taking a 0.0125 mol / L monopotassium phosphate solution as a mobile phase B, eluting according to a specified gradient program, and detecting by adopting an ultraviolet detector to obtain the fingerprint spectrum. By means of the provided construction method of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen, the sequence and the mutual relation of the characteristic peaks can be indicated, and the effect of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen is effectively represented.

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

This invention relates to the field of pharmaceutical analysis technology, specifically to a method for determining the content of deoxycholic acid injection. The detection method employs high-performance liquid chromatography (HPLC) with an ultraviolet (UV) detector. The HPLC chromatographic conditions are as follows: the column is an octadecylsilane-bonded silica column; the column dimensions are 4.6 × 150 mm, and the packing diameter is 3 μm; the detection wavelength is 220 nm; the mobile phase is 0.1% formic acid aqueous solution – 0.1% formic acid acetonitrile solution (42:58). Compared with existing technologies, UV detectors are more readily available than CAD detectors. This invention achieves quantitative detection of deoxycholic acid injection content by controlling the HPLC conditions of the UV detector. This method exhibits baseline stability, good specificity, accuracy, and precision, and is of significant importance for the quality control of deoxycholic acid injection.

This invention belongs to the field of traditional Chinese medicine preparation detection technology, specifically relating to a method for constructing a fingerprint spectrum of Shuangling Zhixie granules and performing quality detection. Utilizing ultra-high performance liquid chromatography (UHPLC), specifically, it includes using octadecylsilane-bonded silica gel as the column packing material, acetonitrile as mobile phase A, and phosphoric acid solution as mobile phase B, performing gradient elution, and using a UV detector to detect and obtain chromatograms. The chromatograms are then processed using fingerprinting software to obtain fingerprint spectra of multiple batches of Shuangling Zhixie granules. The fingerprint spectra of Shuangling Zhixie granules are then imported into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, and after multi-point correction, a control fingerprint spectrum of Shuangling Zhixie granules is generated using the average method. The fingerprint spectrum of the Shuangling Zhixie granule sample to be tested is compared with the control fingerprint spectrum of Shuangling Zhixie granules, and the similarity is calculated. This method can more comprehensively and effectively evaluate and control the quality of Shuangling Zhixie granules; the method is simple, stable, and has good repeatability.

This invention relates to the field of pharmaceutical analysis and detection technology, and proposes a method for detecting related substances in methylcobalamin tablets. The method employs reversed-phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet (UV) detector. The chromatographic conditions of the RHPLC-UV detector method include: mobile phases A and B; mobile phase A is a 92:8 mixture of potassium dihydrogen phosphate solution and acetonitrile, and mobile phase B is a 92:8 mixture of acetonitrile and water; gradient elution is used. This technical solution solves the problem of inaccurate detection of related substances in methylcobalamin tablets in related technologies.

The utility model relates to the technical field of recombinant protein chromatography detection, in particular to recombinant protein batch chromatography detection equipment. A batch chromatography detection mechanism composed of a material tank, a material injection pump, a batch conveying pipe frame, a supporting plate, a sealing cover, a chromatographic column, a UV detector and a collecting tank is jointly mounted among a placement seat, a cross-shaped supporting plate frame I, a cross-shaped supporting plate frame II and a support; according to the present invention, the three chromatographic columns are arranged, such that the equipment can simultaneously perform separation detection on the recombinant protein in the three chromatographic columns during the one-time separation and purification process so as to effectively reduce the separation and purification time of the batch recombinant protein; the large-batch conveying pipe frame is communicated with the chromatographic columns on the different branch plates on the second cross-shaped supporting plate frame, it can be guaranteed that one set of chromatographic columns can be continuously used when the other set of chromatographic columns are blocked, and therefore the continuity of the large-batch recombinant protein separation and purification process is guaranteed, and the large-batch recombinant protein separation and purification efficiency is higher.

The present application relates to a food detection method, and particularly to a method for determining calcium beta-hydroxy-beta-methylbutyrate in high-protein food. 18 The sample is extracted by methanol water ultrasonic extraction, purified by PXC solid phase extraction column, separated by HPLC, eluted by acetonitrile-0.02 mol / L potassium dihydrogen phosphate solution as the mobile phase at a flow rate of 1.0 mL / min, detected by a UV detector, and quantified by an external standard method. The mass concentration of calcium beta-hydroxy-beta-methylbutyrate in the range of 0.020-2.00 mg / mL has a good linear relationship with the peak area, the correlation coefficient is 0.9993, the quantitative limit of special medical purpose formula food is 0.4 g / 100g, and the detection limit is 0.2 g / 100g; the quantitative limit of sports nutrition food is 1.0 g / 100g, and the detection limit is 0.5 g / 100g. The standard addition recovery is between 92.9% and 104%, and the relative standard deviation is 0.94%-3.93%. The method has simple pretreatment, good purification effect and high specificity, and can meet the content determination of calcium beta-hydroxy-beta-methylbutyrate in special medical purpose formula food and sports nutrition food.

The invention provides a method for detecting related components in dipalmitoyl phosphatidylcholine and application of the method, and relates to the technical field of substance analysis. Wherein the related components comprise one or more of palmitic acid, 1-palmitoyl-sn-glycerol-3-phosphorylcholine, glycerophosphorylcholine and imidazole. The invention further discloses a preparation method of the pharmaceutical composition. According to the detection method, related components are separated by adopting a high performance liquid chromatography, a chromatographic column is a hydrophilic interaction chromatographic column, n-hexane and isopropanol form a mobile phase A, methanol is taken as a mobile phase B, and the related components are separated and determined by adopting gradient elution and an evaporative light-scattering detector connected in series with an ultraviolet detector. And obtaining a chromatogram and calculating the content of related components by using an external standard method. The detection method has the characteristics of strong specificity, good separation degree, good precision, high accuracy, simplicity, convenience and rapidness in operation and the like, and can be effectively used for controlling related substances of process intermediates and final products of a dipalmitoyl phosphatidylcholine production process.

This invention relates to the field of pharmaceutical analysis technology, specifically to a method for determining the content of L-2-aminobutyric acid (GABA), a degradation impurity, in a concentrated solution of levetiracetam for injection. The method includes the following steps: diluting the concentrated levetiracetam for injection with a 50% acetonitrile aqueous solution, and then determining the content using high-performance liquid chromatography (HPLC) with a UV detector. C0.05 is also used. 18 The chromatographic column uses an aqueous solution of ammonium dihydrogen phosphate and acetonitrile as the mobile phase. This invention provides an accurate method for determining the degradation impurity L-2-aminobutyric acid in levetiracetam, which is highly specific, sensitive, accurate, and easy to operate, effectively controlling the quality of concentrated levetiracetam injection solutions.

The invention discloses a method for analyzing and determining 4-nonylphenol, bisphenol A and pentachlorophenol in water, which comprises the following steps of: eluting impurities in a solid-phase extraction column by using a reagent, adding a water sample into the solid-phase extraction column, leaving a compound to be detected in a filler of the solid-phase extraction column, discarding water, eluting the compound to be detected in the filler by using an organic reagent, and detecting the content of 4-nonylphenol, bisphenol A and pentachlorophenol in the solid-phase extraction column. The method comprises the following steps of: adding 4-nonylphenol and bisphenol A into an organic solvent, concentrating, separating through liquid chromatography, detecting pentachlorophenol through an ultraviolet detector, detecting 4-nonylphenol and bisphenol A through a fluorescence detector, and determining nature and quantifying peak area through retention time of an ultraviolet spectrogram and a fluorescence spectrogram. The method overcomes the defect that three traditional compounds need to be independently analyzed and determined, is simple and convenient to operate and easy to implement, does not need expensive materials, can be used for obtaining reagent consumables in situ, saves the manpower and material resource cost, and improves the efficiency of analysis and determination operation.

The invention mainly relates to the technical field of drug analysis and detection, in particular to an impurity detection method of a compound amino acid injection. High performance liquid chromatography is adopted for detection, and chromatographic conditions are as follows: a chromatographic column adopts octadecylsilane chemically bonded silica as a filler; the wavelength of a UV detector is 210 to 225 nm; mobile phases: a mobile phase A: a sodium dihydrogen phosphate solution with a pH value of 4.0-6.5 and methanol; a mobile phase B: acetonitrile and water; the elution mode is gradient elution; the impurities comprise a tryptophan impurity B, a tryptophan impurity C, a tryptophan impurity D, a tryptophan impurity E, a tryptophan impurity H, a tryptophan impurity G and indole-3-formaldehyde. The detection method disclosed by the invention is good in specificity and high in accuracy; different detection methods do not need to be adopted for separate detection, multiple impurities in the preparation can be detected at the same time, and the detection frequency is reduced; the detection time is shortened and the detection cost is saved; a reliable method is provided for the preparation process of the compound amino acid injection and the quality control of the product, and the medication safety of a patient is improved.

The application discloses a detection method of indole-3-methanal in compound amino acid injection, and belongs to the field of analytical chemistry. The detection method adopts high performance liquid chromatography, and the chromatographic conditions include: a chromatographic column with octadecylsilane bonded silica as a filler, isocratic elution with a mobile phase, detection by a UV detector with a detection wavelength of 301-311 nm, a flow rate of the mobile phase of 0.6-1.0 mL / min, a sample injection amount of 50-150 muL, a column temperature of 30-40 DEG C, and a mobile phase of a mixed solution of acetonitrile and phosphate buffer solution. The application selects the proportion of the mobile phase, pH, flow rate, column temperature, wavelength and sample injection amount and other chromatographic conditions in the light of the low response and small polarity of the impurity by using high performance liquid chromatography, and the method is verified systematically, and has the advantages of strong specificity, high sensitivity, good precision, strong durability and the like, and can qualitatively or quantitatively detect indole-3-methanal in the compound amino acid injection, so that the effectiveness and safety of the related products are improved.

A curing agent for epoxy resins, which contains an aromatic amine compound (A-1) and an aromatic amine adduct (A-2) that is a reaction product of an aromatic amine compound and a reactive compound having a functional group capable of reacting with the aromatic amine compound, the aromatic amine adduct (A-2) contains an aromatic amine adduct (A-2-1) obtained by adding one molecule of a reactive compound to one molecule of an aromatic amine compound (A-1), and the ratio (A-1 / A-2-1) of the aromatic amine compound to the aromatic amine adduct (A-2-1) obtained by liquid chromatography (UV detector) is 0.50-2.00.

The invention provides a method for preparing high-purity isoquercitrin from polygonum viviparum, which comprises the following steps: sequentially carrying out water extraction impurity removal and alcohol extraction on a polygonum viviparum raw material to obtain an alcohol extract; decolorizing the alcohol extract, then adjusting the pH value to 9-11 to precipitate and remove tannin, and then carrying out vacuum concentration to obtain a general flavone crude product; performing acid hydrolysis on the crude product, performing separation and purification by adopting high-pressure preparative liquid chromatography, performing online monitoring by utilizing an ultraviolet detector, and obtaining a fraction rich in isoquercitrin by accurately controlling starting and ending points of fraction collection; and concentrating under reduced pressure, washing with water and drying to obtain the product. By optimizing the pretreatment and chromatographic separation conditions, the purity and yield of the product are synchronously improved, the HPLC (High Performance Liquid Chromatography) purity of the obtained isoquercitrin reaches 98% or above, and the method is low in process operation cost, environment-friendly and suitable for large-scale production.

This invention belongs to the field of pharmaceutical analysis technology, specifically relating to a method for constructing characteristic chromatograms of Dajianzhong Decoction and a method for determining the content of its effective components. The construction method includes the preparation of a test solution, and under specific conditions, characteristic chromatograms 1 and 2 are obtained using UPLC-UV-ELSD chromatography. Characteristic chromatogram 1, obtained under ultra-high performance liquid chromatography and a UV detector, is specific to dried ginger and roasted Sichuan pepper, with characteristic peaks mainly consisting of gingerol components in dried ginger and amide alkaloids in roasted Sichuan pepper. Characteristic chromatogram 2, obtained under ultra-high performance liquid chromatography and an evaporative light scattering detector, is specific to ginseng, consisting entirely of ginsenosides, thus solving the problem of large differences in detection wavelengths and mutual interference between the two major categories of components.

This invention provides an optical device for real-time detection of H2O2 concentration in air, belonging to the field of gas detection technology. The device uses a deep ultraviolet LED as the light source, and the beam is split by a prism to form a dual-path: beam 1 passes directly through the detection cell, while beam 2 enters the detection cell after forming a fixed optical path difference through a sample cell consisting of a quartz crystal. The two beams share the same UV detector to collect light intensity, and the intensity ratio is used to offset systematic errors such as light source fluctuations and window contamination. The concentration is calculated using Lambert-Beer's law. This device has a detection accuracy of ≤±1.5%, a response time of ≤1s, a core component lifespan of ≥10,000 hours, and a calibration cycle extended to 6 months. It can achieve real-time continuous monitoring in industrial workshops, environmental monitoring, and other scenarios, and features high specificity, strong stability, and low maintenance costs.

The invention relates to the technical field of chemical analysis, and discloses a high performance liquid chromatography analysis method of N-acetyl-glucosamine and N-trifluoroacetyl-glucosamine, which comprises the following steps: dissolving a test sample by using high-proportion acetonitrile as a solvent, using silica gel bonded diol group as a stationary phase, using acetonitrile, methanol and water as mobile phases, and carrying out gradient elution to obtain the high performance liquid chromatography analysis method of N-acetyl-glucosamine and N-trifluoroacetyl-glucosamine. And the ultraviolet detector is used for detection. According to the method for detecting the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine, the retention of the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine is good, the chromatographic peak pattern is symmetrical, and the method is good in specificity, high in precision and accuracy and capable of being efficiently and visually used for identifying the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine and measuring the content of the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine.

The invention relates to the field of drug detection and analysis, in particular to a method for detecting degraded impurities such as carboxylic acids and aldehydes of chloral hydrate. According to the detection method, high performance liquid chromatography is adopted, octadecyl silane bonded silica gel is adopted as a filling agent, an ultraviolet detector is adopted, and the detection wavelength is 195 nm + / -2 nm. According to the detection method, effective separation of auxiliary material peaks and related substances can be achieved, peak patterns of all peaks are good, the separation degree between impurity peaks and main peaks and the separation degree between the impurity peaks meet the requirements of pharmacopeia, and specificity is good. The method is suitable for drug registration quality research of chloral hydrate and preparations thereof.

The invention discloses an intelligent fully-closed operation CO2 supercritical continuous liquid chromatography system which comprises the following steps: S1, selecting pure CO2 in a supercritical state or CO2 which contains an entrainer and is in a supercritical state as a mobile phase according to the property of a sample; s2, setting a purification process route according to a spectrogram under the basic conditions of walking a single column and reading the spectrogram, correcting software parameters in a man-machine conversation mode, and then starting a program; s3, when the post-column liquid enters the corresponding first-stage tank body, a precise pressure regulating valve between the first-stage tank and the second-stage tank is automatically adjusted according to the pressure set by a program, so that the supercritical solution containing sample components enters the second-stage tank, meanwhile, clean liquid appears in the first-stage tank, and the clean liquid is discharged into the clean liquid first-stage tank. A closed operation process method is applied, an LED strong penetrating UV detector is adopted, intelligent control is achieved, and automatic operation, continuous sample injection and automatic sample injection time and sample injection port selection can be achieved. Due to the two-stage pressure release design, automatic control can be achieved.

The application discloses a quality control method for a swelling and pain relieving composition, which comprises the following steps: A, preparing a receiving side solution; B, using a YUS chamber to prepare a test sample solution, fixing rat skin in a 0.5 cm2 diffusion chamber, with the outer epidermis side as a drug administration side, adding a swelling and pain relieving extract to the drug administration side container, with the inner epidermis side as a receiving side, adding a receiving liquid to the receiving side container to two-thirds of the container, finally covering the top of the two containers with a fresh-keeping film, opening a constant temperature water bath to maintain a constant temperature of 37.5 DEG C, waiting for 36-48 hours, passing through a 0.22 mu m microporous filter membrane, taking the filtrate, and obtaining the test sample solution; C, using a liquid chromatography program to separate and then detecting by a UV detector. The method relies on a high performance liquid chromatograph, extracts transdermal components through a YUS chamber through an isolated rat skin, and forms a new type of quality control method for a swelling and pain relieving composition.

The invention relates to the technical field of pharmaceutical analysis, in particular to a liquid phase detection method for simultaneously detecting 13 ginsenosides. According to the method, chromatographic conditions are controlled: acetonitrile is used as a mobile phase A, a 0.03%-0.08% phosphoric acid aqueous solution is used as a mobile phase B, and gradient elution is carried out; the chromatographic column is GS-120-5-C18-AP 4.6 mm * 250 mm, the column temperature is 30 DEG C to 35 DEG C, and the detection wavelength is 200 nm to 210 nm; according to the method, 13 ginsenosides can be detected at the same time in a short time by matching a common liquid chromatograph with an ultraviolet detector, the peak pattern and separation effect of each saponin are good, the detection efficiency is remarkably improved, and an efficient detection method is provided for screening the content of ginsenoside raw materials and monitoring the synthesis and conversion content of ginsenosides.

This invention relates to the field of detection and analysis technology, and discloses a method for detecting perfluorooctanoic acid (PFOA) in water. The method includes the following steps: filtering a water sample through a filter membrane and collecting the filtrate; using a C18 reversed-phase chromatographic column, with an aqueous solution containing tetrabutylammonium bisulfate as mobile phase A and acetonitrile as mobile phase B, isocratic elution is performed at a detection wavelength of 208-215 nm, and detection is performed using a UV detector or a diode array detector; qualitative analysis is performed based on chromatographic retention time, and quantitative analysis is performed using the external standard method based on chromatographic peak area. This invention, by optimizing the detection wavelength and introducing tetrabutylammonium bisulfate as an ion-pairing reagent, effectively improves the chromatographic peak shape and resolution of PFOA, achieving a correlation coefficient R0 within a wide linear range of 0.5-200 mg / L. 2 The detection limit is >0.999, which meets the detection requirements of most water bodies; the method has good repeatability, with a spiked recovery rate of up to 98.30%-108.35% in complex matrices; it has strong anti-interference ability, and the detection results are stable in the pH range of 4-10, making it suitable for actual water samples under different acid and alkaline conditions.

The invention relates to the technical field of pharmaceutical analysis, discloses an integrated method for one-time determination of main components and purity of tolperidone hydrochloride and p-methyl propiophenone by using reversed-phase high performance liquid chromatography, and is suitable for quality control of raw materials, intermediate products and finished products. According to the method, a mixed phosphate solution-methanol mixed system is taken as a mobile phase A, diethylamine-methanol is taken as a mobile phase B, a gradient elution procedure is adopted on a C18 reversed-phase chromatographic column, and an ultraviolet detector or a diode array detector (DAD) is matched, so that the determination of the main content of tolperidone hydrochloride and the monitoring of the content or residual quantity of methyl propiophenone can be realized. Compared with the prior art, the method has the advantages that the problem of synchronous analysis of main components and key impurities in a tolperidone hydrochloride process and the problem of content and purity determination of a p-methyl propiophenone raw material are solved for the first time, the method has the characteristics of strong specificity, high sensitivity, economy and environmental protection, and reliable technical support is provided for formulation of a medicine quality standard and process optimization.

The present invention addresses the problem of providing a novel form of a phenol compound represented by chemical formula (A1) and having a methoxymethyl group, which can be produced by a production method suitable for industrial mass production, and with which it is possible to reduce the energy required when the same is used by heating and melting a solid thereof. As a means for solving said problem, provided is a powder of a composition containing a phenol compound represented by chemical formula (A1) and having a methoxymethyl group, said powder containing, at the compositional ratio below, a phenol compound represented by chemical formula (A1) and having a methoxymethyl group as a component A1, a compound represented by chemical formula (A2) as a component A2, and a compound represented by chemical formula (A3) as a component A3, wherein the onset temperature of an endothermic peak according to a differential scanning calorimetry analysis is in the range of 50-65°C. [Compositional ratio] The following ranges are the detection area proportions of the components relative to the detection areas of all components detected by liquid chromatography analysis of the composition using a 280-nm wavelength UV detector. Component A1: 83.0-88.0 area%, component A2: 3.5-6.5 area%, and component A3: 0.01-3.0 area%

A photodetector comprising a semiconductor and a 2D material and a method of manufacturing the same. A space charge region is located directly at the surface of the semiconductor. Photons with a small wavelength are immediately within the space charge region once they enter the semiconductor, in which space charge region electron-hole pairs generated by the photons can contribute to the photocurrent. This makes the photodetector a highly efficient UV detector. An interdigitated oxide structure between the semiconductor and the 2D material further enhances the sensitivity of the photodetector to, for example, UV light.

An ultraviolet (UV) room-sterilization device includes an upper portion with germicidal lamps and movable shield walls mounted by multi-hinged arms. The walls move from a closed position to selected open positions to form a shadow area that protects occupants or equipment while permitting disinfection elsewhere. Hinge-position sensors and / or UV detectors generate a live emission map used to classify motion detected by onboard sensors: movement within the shadow is permitted, movement toward its boundary triggers a warning, and movement in exposed regions causes immediate lamp shutoff. A timer provides delayed start and cycle limits with code-based authorization. Embodiments include circumferential or sector lamps, retractable walls slotted into the housing, and floor, tabletop, or handheld form factors, enabling safe, targeted disinfection in occupied spaces.