17 results about "Uv detector" patented technology

The invention relates to the technical field of analysis and detection, and discloses a construction method of a fingerprint spectrum of relevant characteristic peaks of rhizoma coptidis paste and radix rehmanniae recen, and the construction method comprises the following steps: S1, preparing a reference solution and a test solution; and S2, injecting the reference substance solution and the test solution into a high performance liquid chromatograph, adopting a chromatographic column with octadecylsilane chemically bonded silica as a filler, taking acetonitrile as a mobile phase A, taking a 0.0125 mol / L monopotassium phosphate solution as a mobile phase B, eluting according to a specified gradient program, and detecting by adopting an ultraviolet detector to obtain the fingerprint spectrum. By means of the provided construction method of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen, the sequence and the mutual relation of the characteristic peaks can be indicated, and the effect of the fingerprint spectrum of the relevant characteristic peaks of the coptis chinensis paste and the radix rehmanniae recen is effectively represented.

This invention discloses a method and application for extracting soluble collagen from bones unearthed from archaeological sites, belonging to the field of archaeological sample processing technology. Specifically: 1) After pretreatment, the blocky ancient bone sample is dried at low temperature and ground into bone powder; the bone powder is added to an acidic solution for ultrasonic treatment, centrifuged, and the supernatant is filtered to obtain the sample to be loaded onto a column; 2) The sample to be loaded onto the column is subjected to chromatographic separation, using a gradient elution with equilibration buffer and elution buffer, and a UV detector is used to monitor protein peaks, collecting the target protein solution that elutes within 20-30 minutes; 3) Finally, the target protein solution is concentrated and purified using an ultrafiltration tube to obtain soluble collagen. This invention can obtain a sufficient amount of collagen from ancient deteriorated bone samples, significantly improving sample utilization and providing reliable technical support for bioarchaeological research.

This invention relates to the field of pharmaceutical analysis technology, specifically to a method for determining the content of deoxycholic acid injection. The detection method employs high-performance liquid chromatography (HPLC) with an ultraviolet (UV) detector. The HPLC chromatographic conditions are as follows: the column is an octadecylsilane-bonded silica column; the column dimensions are 4.6 × 150 mm, and the packing diameter is 3 μm; the detection wavelength is 220 nm; the mobile phase is 0.1% formic acid aqueous solution – 0.1% formic acid acetonitrile solution (42:58). Compared with existing technologies, UV detectors are more readily available than CAD detectors. This invention achieves quantitative detection of deoxycholic acid injection content by controlling the HPLC conditions of the UV detector. This method exhibits baseline stability, good specificity, accuracy, and precision, and is of significant importance for the quality control of deoxycholic acid injection.

This invention belongs to the field of traditional Chinese medicine preparation detection technology, specifically relating to a method for constructing a fingerprint spectrum of Shuangling Zhixie granules and performing quality detection. Utilizing ultra-high performance liquid chromatography (UHPLC), specifically, it includes using octadecylsilane-bonded silica gel as the column packing material, acetonitrile as mobile phase A, and phosphoric acid solution as mobile phase B, performing gradient elution, and using a UV detector to detect and obtain chromatograms. The chromatograms are then processed using fingerprinting software to obtain fingerprint spectra of multiple batches of Shuangling Zhixie granules. The fingerprint spectra of Shuangling Zhixie granules are then imported into a traditional Chinese medicine chromatographic fingerprint similarity evaluation system, and after multi-point correction, a control fingerprint spectrum of Shuangling Zhixie granules is generated using the average method. The fingerprint spectrum of the Shuangling Zhixie granule sample to be tested is compared with the control fingerprint spectrum of Shuangling Zhixie granules, and the similarity is calculated. This method can more comprehensively and effectively evaluate and control the quality of Shuangling Zhixie granules; the method is simple, stable, and has good repeatability.

This invention relates to the field of pharmaceutical analysis and detection technology, and proposes a method for detecting related substances in methylcobalamin tablets. The method employs reversed-phase high-performance liquid chromatography (RP-HPLC) with an ultraviolet (UV) detector. The chromatographic conditions of the RHPLC-UV detector method include: mobile phases A and B; mobile phase A is a 92:8 mixture of potassium dihydrogen phosphate solution and acetonitrile, and mobile phase B is a 92:8 mixture of acetonitrile and water; gradient elution is used. This technical solution solves the problem of inaccurate detection of related substances in methylcobalamin tablets in related technologies.

The present application relates to a food detection method, and particularly to a method for determining calcium beta-hydroxy-beta-methylbutyrate in high-protein food. 18 The sample is extracted by methanol water ultrasonic extraction, purified by PXC solid phase extraction column, separated by HPLC, eluted by acetonitrile-0.02 mol / L potassium dihydrogen phosphate solution as the mobile phase at a flow rate of 1.0 mL / min, detected by a UV detector, and quantified by an external standard method. The mass concentration of calcium beta-hydroxy-beta-methylbutyrate in the range of 0.020-2.00 mg / mL has a good linear relationship with the peak area, the correlation coefficient is 0.9993, the quantitative limit of special medical purpose formula food is 0.4 g / 100g, and the detection limit is 0.2 g / 100g; the quantitative limit of sports nutrition food is 1.0 g / 100g, and the detection limit is 0.5 g / 100g. The standard addition recovery is between 92.9% and 104%, and the relative standard deviation is 0.94%-3.93%. The method has simple pretreatment, good purification effect and high specificity, and can meet the content determination of calcium beta-hydroxy-beta-methylbutyrate in special medical purpose formula food and sports nutrition food.

This invention relates to the field of pharmaceutical analysis technology, specifically to a method for determining the content of L-2-aminobutyric acid (GABA), a degradation impurity, in a concentrated solution of levetiracetam for injection. The method includes the following steps: diluting the concentrated levetiracetam for injection with a 50% acetonitrile aqueous solution, and then determining the content using high-performance liquid chromatography (HPLC) with a UV detector. C0.05 is also used. 18 The chromatographic column uses an aqueous solution of ammonium dihydrogen phosphate and acetonitrile as the mobile phase. This invention provides an accurate method for determining the degradation impurity L-2-aminobutyric acid in levetiracetam, which is highly specific, sensitive, accurate, and easy to operate, effectively controlling the quality of concentrated levetiracetam injection solutions.

The application discloses a detection method of indole-3-methanal in compound amino acid injection, and belongs to the field of analytical chemistry. The detection method adopts high performance liquid chromatography, and the chromatographic conditions include: a chromatographic column with octadecylsilane bonded silica as a filler, isocratic elution with a mobile phase, detection by a UV detector with a detection wavelength of 301-311 nm, a flow rate of the mobile phase of 0.6-1.0 mL / min, a sample injection amount of 50-150 muL, a column temperature of 30-40 DEG C, and a mobile phase of a mixed solution of acetonitrile and phosphate buffer solution. The application selects the proportion of the mobile phase, pH, flow rate, column temperature, wavelength and sample injection amount and other chromatographic conditions in the light of the low response and small polarity of the impurity by using high performance liquid chromatography, and the method is verified systematically, and has the advantages of strong specificity, high sensitivity, good precision, strong durability and the like, and can qualitatively or quantitatively detect indole-3-methanal in the compound amino acid injection, so that the effectiveness and safety of the related products are improved.

The invention provides a method for preparing high-purity isoquercitrin from polygonum viviparum, which comprises the following steps: sequentially carrying out water extraction impurity removal and alcohol extraction on a polygonum viviparum raw material to obtain an alcohol extract; decolorizing the alcohol extract, then adjusting the pH value to 9-11 to precipitate and remove tannin, and then carrying out vacuum concentration to obtain a general flavone crude product; performing acid hydrolysis on the crude product, performing separation and purification by adopting high-pressure preparative liquid chromatography, performing online monitoring by utilizing an ultraviolet detector, and obtaining a fraction rich in isoquercitrin by accurately controlling starting and ending points of fraction collection; and concentrating under reduced pressure, washing with water and drying to obtain the product. By optimizing the pretreatment and chromatographic separation conditions, the purity and yield of the product are synchronously improved, the HPLC (High Performance Liquid Chromatography) purity of the obtained isoquercitrin reaches 98% or above, and the method is low in process operation cost, environment-friendly and suitable for large-scale production.

This invention belongs to the field of pharmaceutical analysis technology, specifically relating to a method for constructing characteristic chromatograms of Dajianzhong Decoction and a method for determining the content of its effective components. The construction method includes the preparation of a test solution, and under specific conditions, characteristic chromatograms 1 and 2 are obtained using UPLC-UV-ELSD chromatography. Characteristic chromatogram 1, obtained under ultra-high performance liquid chromatography and a UV detector, is specific to dried ginger and roasted Sichuan pepper, with characteristic peaks mainly consisting of gingerol components in dried ginger and amide alkaloids in roasted Sichuan pepper. Characteristic chromatogram 2, obtained under ultra-high performance liquid chromatography and an evaporative light scattering detector, is specific to ginseng, consisting entirely of ginsenosides, thus solving the problem of large differences in detection wavelengths and mutual interference between the two major categories of components.

This invention provides an optical device for real-time detection of H2O2 concentration in air, belonging to the field of gas detection technology. The device uses a deep ultraviolet LED as the light source, and the beam is split by a prism to form a dual-path: beam 1 passes directly through the detection cell, while beam 2 enters the detection cell after forming a fixed optical path difference through a sample cell consisting of a quartz crystal. The two beams share the same UV detector to collect light intensity, and the intensity ratio is used to offset systematic errors such as light source fluctuations and window contamination. The concentration is calculated using Lambert-Beer's law. This device has a detection accuracy of ≤±1.5%, a response time of ≤1s, a core component lifespan of ≥10,000 hours, and a calibration cycle extended to 6 months. It can achieve real-time continuous monitoring in industrial workshops, environmental monitoring, and other scenarios, and features high specificity, strong stability, and low maintenance costs.

The invention relates to the technical field of chemical analysis, and discloses a high performance liquid chromatography analysis method of N-acetyl-glucosamine and N-trifluoroacetyl-glucosamine, which comprises the following steps: dissolving a test sample by using high-proportion acetonitrile as a solvent, using silica gel bonded diol group as a stationary phase, using acetonitrile, methanol and water as mobile phases, and carrying out gradient elution to obtain the high performance liquid chromatography analysis method of N-acetyl-glucosamine and N-trifluoroacetyl-glucosamine. And the ultraviolet detector is used for detection. According to the method for detecting the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine, the retention of the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine is good, the chromatographic peak pattern is symmetrical, and the method is good in specificity, high in precision and accuracy and capable of being efficiently and visually used for identifying the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine and measuring the content of the N-acetyl-glucosamine and the N-trifluoroacetyl-glucosamine.

The invention relates to the field of drug detection and analysis, in particular to a method for detecting degraded impurities such as carboxylic acids and aldehydes of chloral hydrate. According to the detection method, high performance liquid chromatography is adopted, octadecyl silane bonded silica gel is adopted as a filling agent, an ultraviolet detector is adopted, and the detection wavelength is 195 nm + / -2 nm. According to the detection method, effective separation of auxiliary material peaks and related substances can be achieved, peak patterns of all peaks are good, the separation degree between impurity peaks and main peaks and the separation degree between the impurity peaks meet the requirements of pharmacopeia, and specificity is good. The method is suitable for drug registration quality research of chloral hydrate and preparations thereof.

The invention relates to the technical field of pharmaceutical analysis, in particular to a liquid phase detection method for simultaneously detecting 13 ginsenosides. According to the method, chromatographic conditions are controlled: acetonitrile is used as a mobile phase A, a 0.03%-0.08% phosphoric acid aqueous solution is used as a mobile phase B, and gradient elution is carried out; the chromatographic column is GS-120-5-C18-AP 4.6 mm * 250 mm, the column temperature is 30 DEG C to 35 DEG C, and the detection wavelength is 200 nm to 210 nm; according to the method, 13 ginsenosides can be detected at the same time in a short time by matching a common liquid chromatograph with an ultraviolet detector, the peak pattern and separation effect of each saponin are good, the detection efficiency is remarkably improved, and an efficient detection method is provided for screening the content of ginsenoside raw materials and monitoring the synthesis and conversion content of ginsenosides.

This invention relates to the field of detection and analysis technology, and discloses a method for detecting perfluorooctanoic acid (PFOA) in water. The method includes the following steps: filtering a water sample through a filter membrane and collecting the filtrate; using a C18 reversed-phase chromatographic column, with an aqueous solution containing tetrabutylammonium bisulfate as mobile phase A and acetonitrile as mobile phase B, isocratic elution is performed at a detection wavelength of 208-215 nm, and detection is performed using a UV detector or a diode array detector; qualitative analysis is performed based on chromatographic retention time, and quantitative analysis is performed using the external standard method based on chromatographic peak area. This invention, by optimizing the detection wavelength and introducing tetrabutylammonium bisulfate as an ion-pairing reagent, effectively improves the chromatographic peak shape and resolution of PFOA, achieving a correlation coefficient R0 within a wide linear range of 0.5-200 mg / L. 2 The detection limit is >0.999, which meets the detection requirements of most water bodies; the method has good repeatability, with a spiked recovery rate of up to 98.30%-108.35% in complex matrices; it has strong anti-interference ability, and the detection results are stable in the pH range of 4-10, making it suitable for actual water samples under different acid and alkaline conditions.

A photodetector comprising a semiconductor and a 2D material and a method of manufacturing the same. A space charge region is located directly at the surface of the semiconductor. Photons with a small wavelength are immediately within the space charge region once they enter the semiconductor, in which space charge region electron-hole pairs generated by the photons can contribute to the photocurrent. This makes the photodetector a highly efficient UV detector. An interdigitated oxide structure between the semiconductor and the 2D material further enhances the sensitivity of the photodetector to, for example, UV light.

An ultraviolet (UV) room-sterilization device includes an upper portion with germicidal lamps and movable shield walls mounted by multi-hinged arms. The walls move from a closed position to selected open positions to form a shadow area that protects occupants or equipment while permitting disinfection elsewhere. Hinge-position sensors and / or UV detectors generate a live emission map used to classify motion detected by onboard sensors: movement within the shadow is permitted, movement toward its boundary triggers a warning, and movement in exposed regions causes immediate lamp shutoff. A timer provides delayed start and cycle limits with code-based authorization. Embodiments include circumferential or sector lamps, retractable walls slotted into the housing, and floor, tabletop, or handheld form factors, enabling safe, targeted disinfection in occupied spaces.