The invention relates to the technical field of biomedical sensing and wearable health monitoring, and discloses a photoelectrochemical sensing microneedle, a preparation method thereof, a
monitoring system and a detection method. The sensing microneedle comprises a swellable dual-network hydrogel microneedle substrate, a three-dimensional conductive network and a
semiconductor nanostructure, wherein the three-dimensional conductive network is composed of high-length-
diameter-ratio
metal nanowires with a local surface
plasma resonance effect, and the
semiconductor nanostructure is compounded on the surfaces of the
metal nanowires and forms a Schottky
heterojunction. The mechanical constraint of the rigid
polymer skeleton and the high length-
diameter ratio of the
metal nanowire cooperate to ensure the continuity of the conductive network in a swelling state. Under the
irradiation of exciting light,
hot electrons are migrated to the surface of a
semiconductor through a Schottky junction built-in
electric field to generate reactive
oxygen free radicals, non-enzymatic
catalytic oxidation is carried out on a target
analyte in
interstitial fluid under
zero bias voltage or low bias
voltage, a
photocurrent signal is generated, and integration of minimally invasive sampling and in-situ high-selectivity detection is realized.