48 results about "Chorioallantoic membrane" patented technology

The present invention relates to a novel in vivo assay for quantitating intravasation of cancer cells based on a highly sensitive polymerase chain reaction (PCR) utilized in combination with a chick embryo chorioallantoic membrane (CAM) assay. The assay of the invention provides a method for measuring the metastatic potential of cancer cells. The assay also provides a drug screening assay for identification of agents having anti-metastatic activity.

The invention discloses a tuna ground meat polypeptide angiogenesis inhibiting factor as well as a preparation method and an application thereof. The amino acid sequence of the polypeptide angiogenesis inhibiting factor is Trp-Val-Thr-Gly-Ile (WVTGI), and ESI / MS (electrospray ionization / mass spectrometry) detection shows that a molecular ion peak (m / z) is 575.42Da ([M+H]+). The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for effectively inhibiting angiogenesis of chorioallantoic membrane (CAM) of chicken embryo and multiplication of prostate cancer cell DU-145. The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for preparing a medicine used for inhibiting angiogenesis and preventing and treating prostate cancer.

The invention provides a novel medical application of arctigenin, belonging to the field of medicine. According to the application of arctigenin in treating angiogenesis diseases provided by the invention, the arctigenin is applied to treating angiogenesis diseases, a good angiogenesis inhibiting effect is shown in a chorioallantoic membrane model test of a chicken embryo, which reminds that the arctigenin can be applied to treatment or auxiliary treatment of angiogenetic diseases. The medical application of arctigenin which inhibits angiogenesis reminds that the arctigenin has wide medical application prospect in treating diseases such as tumor and other pathological angiogenesis diseases such as diabetic retinopathy, psoriasis, atherosclerosis, rheumatoid arthritis and the like.

The invention discloses a method for detecting a cosmetic and raw materials of the cosmetic. The method includes the following steps that a plurality of animal fertilized egg embryos are taken, an opening is formed in the air chamber end of each egg shell, normal saline is added into the openings, and air chamber membranes are torn to expose chorioallantoic membranes; teflon rings are fixedly attached to the chorioallantoic membranes of the animal fertilized egg embryos, and tested regions are formed by in-ring parts of the teflon rings; a plurality of to-be-tested samples are taken and added to the tested regions, then the outer sides of the animal fertilized egg embryos are covered with preservative films, and then the animal fertilized egg embryos are arranged in an incubator; after a period of time, the animal fertilized egg embryos are taken out of the incubator, changes of blood vessels of the in-ring chorioallantoic membranes and changes of blood vessels of the out-ring chorioallantoic membranes are observed and compared, injury grades are determined, and stimulation grades of the to-be-tested samples are determined according to the injury grades. According to the method, the human body is not treated as an experimental subject, safety risks are reduced, and repeated testing is convenient.

The invention provides a preparation method for avian reovirus virus water-in-oil-in-water type inactivated vaccines. The preparation method comprises the following steps: preparation of chicken embryo fibroblast, cultivation of viruses, inactivation of the viruses, emulsification of the vaccines, and the like. Compared with the prior art, locally separated avian reoviruses are adopted, so that the immunogenicity is good, and the relatively high protection rate is adopted; the chicken embryo fibroblast is adopted to replace the utilization of a chicken embryo chorioallantoic membrane in a inoculation method for breeding the avian reoviruses, so that the vaccine preparation cost is saved, the mass discrepancy between each batch is lowered, the yield is high, and the viral titer and the vaccine quality are improved; the vaccines are subjected to emulsification by using a homogenizer, the prepared vaccines are water-in-oil-in-water type; compared with traditional water-in-oil type vaccines, the vaccines provided by the invention have low viscosity, small granularity, and easy absorption; surface active agent compositions are not required to be added in the preparation method by selecting and using an adjuvant, so that the prepared vaccines have good safety, and avoid side effects caused by difficult absorption of a traditional oil adjuvant.

The invention discloses a method for detecting and evaluating conjunctival irritation and mucous membrane irritation by using chicken embryo vascular system, comprising the following steps: (1) preparation of chorioallantoic membrane; (2) conjunctival irritation test; (3) sticking mold Stimulation test; (4) prediction model and classification, the present invention uses the standardized chicken embryo as the test system, and its vascular system at different stages of development is similar to the human eye conjunctiva and oral submucosa tissue, and the chicken embryo has a high degree of standardization, stable source and batch The difference between blood vessels is small, the vascular system has biological activity and function, the test system is simple and cheap, no special equipment is required, and the detection method is fast, sensitive and universal. The invention can detect and evaluate the conjunctival irritation and mucous membrane irritation of the substance to be tested from both qualitative and quantitative aspects. The method of the present invention can also replace living animals and human volunteers, and be directly used for the detection of conjunctival irritation and mucosal irritation of substances such as chemicals or cosmetics, and rapid screening, classification and identification thereof.

The invention belongs to an application of dandelion polysaccharides in preparation of drugs for inhibiting angiogenesis of hepatocellular carcinoma cells; the effects of the dandelion polysaccharideson angiogenesis are observed by scratch tests, eanaliculization tests and chick embryo chorioallantoic membrane angiogenesis tests, and the effects of the dandelion polysaccharides on the angiogenesis of subcutaneous tumors of H22 hepatocellular carcinoma cell-bearing mice are studied by immunohistochemistry; qPCR, Western blot and ELISA are used for detecting the expression of angiogenesis-related factor mRNA and proteins at cellular and animal levels respectively; the dandelion polysaccharides can be effectively proved to play an anti-tumor role by inhibiting tumor angiogenesis, and tumor angiogenesis can be inhibited in vivo and in vitro by down-regulating the expression of tumor cells VEGF and VEGF-2. The dandelion polysaccharides have the advantage of effectively inhibiting tumor angiogenesis to achieve anti-tumor effect.

The present invention relates to an amidopyridinol derivative or a pharmaceutically acceptable salt thereof, and a pharmaceutical composition including an amidopyridinol derivative or a pharmaceutically acceptable salt thereof as an active component. An amidopyridinol derivative represented by Chemical Formula 1 or a pharmaceutically acceptable salt thereof has an excellent antiangiogenic effect in a chorioallantoic membrane model, and thus is useful as an agent for preventing or treating diseases associated with angiogenesis, such as macular degeneration or arthritis, and also has an excellent colitis-inhibitory effect in a model of inflammatory bowel diseases, and thus is useful as an agent for preventing or treating inflammatory bowel diseases. Upon inoculation of lung cancer cells in a chorioallantoic membrane model, the amidopyridinol derivative of Chemical Formula 1 or the pharmaceutically acceptable salt thereof inhibits angiogenesis and tumor growth that are caused by tumorigenesis, and also inhibits the activity of cathepsin S that plays an important role in metastasis and invasion of cancer, and thus is useful as an inhibitor of cancer growth and metastasis.

Disclosed is a pharmaceutical composition including a bicyclic pyridinol derivative as an active ingredient for preventing or treating diseases caused by angiogenesis. The bicyclic pyridinol derivative expressed by Chemical Formula 1 can be effectively used as a drug for preventing and treating diseases caused by angiogenesis since it inhibits neovascularization in a chorioallantoic membrane model.

The invention discloses application of a fulvic acid salt in preparation of a medicament for promoting angiogenesis. A clinical research shows that angiogenesis opens up a new therapy pathway for revascularization of ischemic angiopathy. An experiment result shows that the fulvic acid salt disclosed by the invention has a human umbilical vein endothelial cell proliferation promoting activity and a chick embryo chorioallantoic membrane angiogenesis promoting activity, and can be applied to promoting angiogenesis therapy of ischemic angiopathy (for example, a peripheral arterial ischemic vascular disease, thromboangiitis obliterans, a foot disease in diabetes and the like).

The invention relates to application of Pluronic F-127 solution in chick embryo chorioallantoic membrane experiment as medicine carrying material. The application includes the steps of setting the Pluronic F-127 solution at 4 DEG C through still-setting method to dissolve the solution, configurating biological isotonic solution of certain concentration, and thus the medicine carrying material is obtained. The Pluronic F-127 solution is in liquid state in low temperature, and thus the solution can be mixed with tested medicine conveniently, and possibility of temperature-sensitive medicine degeneration is reduced to the greatest extent. The Pluronic F-127 solution is transparent when the solution is in solid state in high temperature, and thus chick embryo chorioallantoic membrane does not deform when the Pluronic F-127 solution is used and observation is convenient.

The invention discloses a chicken embryo culture method of Portunus tritubereulatus reovirus and is characterized by comprising the following steps of: using SPF chicken embryo as an in vitro culture system of Portunus tritubereulatus reovirus, selecting healthy chicken embryo of 3 and 4 days-old, inoculating a prepared Portunus tritubereulatus reovirus inoculated solution onto a chorioallantoic membrane of the chicken embryo, continuously culturing until the chicken embryo is dead, collecting dead chicken embryo cultured for 3-10 days, separating the chorioallantoic membrane and amniotic fluid, mixing the chorioallantoic membrane and the amniotic fluid, homogenizing the mixture, centrifuging and filtering to obtain a supernatant of Portunus tritubereulatus reovirus. The invention has the following advantages: a more efficient and adaptive Portunus tritubereulatus reovirus in vitro culture method is provided for rapid and effective in vitro subcultring of the virus; and the method overcomes the defect that there is no adaptive culture system during the culture process of Portunus tritubereulatus reovirus, fills the blank of chicken embryo culture system of crab virus and has laid a foundation for further research and application.

The invention relates to the field of medicinal chemistry and particularly relates to a class of N-[1, 3, 6-tri-O-acetyl-4-O-(2, 3, 4, 6-quadri-O-acetyl)-beta-D-galactosyl]-2-deoxy-beta-D-glucosyl group]-3-(substituted phenyl) acrylamide (I), as well as a preparation method and an application thereof in restriction of vascular endothelial cell proliferation and chick embryo chorioallantoic membrane neovascularization.

The invention relates to the technical field of biology, in particular to a MDPV (Muscovy duck parvovirus) infectious clone construction and saving method. The method includes: cloning an intact MDPV genome into vector plasmids pBSKN to obtain recombinant plasmids; mixing the recombinant plasmids with a transfection agent, and performing inoculation of nonimmune muscovy duck's embryon through the chorioallantoic membrane to save the recombinant plasmids in the muscovy duck's embryon, wherein a pBSKN vector is obtained by replacement of original EcoRV enzyme digestion sites of commercialized plasmid pBluescript II (SK) multiple clone sites with NcoI sites. Generated infectious viruses are lethal to the muscovy duck's embryon, and saving viruses and parent viruses are similar in lethality rate of young muscovy ducks. The saving method is simple, convenient and efficient, problems of low efficiency and complexity caused by transfection of primary cells are avoided, and potential application values in MDPV reverse genetic research and vaccine creation are achieved.

A method or composition including 6-aminopyridin-3-ol derivatives or pharmaceutically acceptable salts thereof for treating a disease caused by angiogenesis, the 6-aminopyridin-3-ol derivatives represented by Formula 1 or the pharmaceutically acceptable salts thereof have excellent neoangiogenesis inhibition effects in the chorioallantoic membrane model, and are suitable for use as a drug for the treatment of disease caused by angiogenesis.

The present invention provides a novel angiogenesis inhibitor, LK68 whose amino acid sequence is identical with the human apolipoprotein (a) kringle domains IV36, IV37 and V38, a cDNA sequence encoding the LK68, a recombinant expression vector comprising the cDNA, a recombinant microorganism transformed with the recombinant expression vector and a novel use of the LK68 as an anticancer agent and a method for treating angiogenesis-mediated disease. LK68, LK6, LK7 and LK8 exhibit inhibitory activities on the cultured endothelial cell proliferation as well as on the endothelial cell migration. LK68 and its single kringles also inhibit the normal development of capillaries in the chick embryo chorioallantoic membrane (CAM). It was also showed that systemic administration of LK68 causes the inhibition of primary tumor growth, which is correlated with a suppression of tumor-induced angiogenesis. Accordingly, LK68 protein, its single kringles or their functional equivalents may be applied for the development of a potent anti-cancer agent, which is highly effective for angiogenesis-mediated diseases covering cancer, rheumatoid arthritis, psoriasis, ocular angiogenic disease, etc.

The invention relates to the technical field of biochemical engineering, in particular to cardiac glycoside with anti-angiogenesis activity as well as a preparation method and application thereof. The cardiac glycoside with the anti-angiogenesis activity is a compound extracted and separated from digitalis lanceolata leaves. The compound has nano-molar concentration level proliferation inhibition activity on non-small cell lung cancer cells A549, cervical cancer cells Hela and breast cancer cells MCF-7. In addition, the compound can remarkably inhibit new angiogenesis on a chick embryo chorioallantoic membrane model, so that candidate drugs can be provided for developing novel anti-tumor angiogenesis drugs.

The invention relates to the technical field of biochemical engineering, in particular to cardiac glycoside with anti-angiogenesis activity and a preparation method and application thereof. According to the invention, the cardiac glycoside compound with anti-angiogenesis activity is separated from a medicinal plant digitalis lanata, and the compound has nano-molar concentration level proliferation inhibition activity on non-small cell lung cancer cells, cervical cancer cells and breast cancer cells, and can be used for preparing anti-tumor drugs; moreover, the compound can significantly inhibit new angiogenesis on a chick embryo chorioallantoic membrane model, and can be used for preparing anti-angiogenesis drugs; meanwhile, the compound is directly extracted and separated from medicinal plants which can be planted on a large scale, the raw materials are easy to obtain, large-scale production can be achieved, the social benefit is remarkable, and the cost is low.

The present invention relates to a composition and its application, said composition comprising peach gum extract and cactus extract. The composition of the present invention has a significant effect of antagonizing the irritation of surfactants. Efficacy evaluation experiments confirmed that the composition of the present invention has a significant inhibitory effect on erythrocyte hemolysis caused by surfactants, significantly reduces the irritation of the chicken embryo chorioallantoic membrane caused by surfactants, and can effectively improve the skin texture caused by surfactants. It can significantly improve the disorder of skin lipid arrangement caused by surfactants, and significantly reduce the adverse reactions of different surfactants to human skin.

The present invention relates to uses of a Sphyrna lewini cartilage angiogenesis inhibitory factor, namely, the Sphyrna lewini cartilage angiogenesis inhibitory factor is used to prepare health foods or functional additives for angiogenesis inhibition and tumor prevention. The amino acid sequence and ESI-MS detected molecular weight of the angiogenesis inhibitory factor is Pro-Asp-Tyr-Lys-Phe-Lys and 796.90 Da, respectively. The angiogenesis inhibitor factor can effectively inhibit angiogenesis in chick embryo chorioallantoic membrane, and significantly inhibits the expressions of three angiogenic factors including vascular endothelial growth factor, basic fibroblast growth factor and platelet derived growth factor in lung cancer tissues of mice bearing Lewis lung cancer, so that the angiogenesis inhibitor factor can be used to prepare health foods or functional additives for angiogenesis inhibition and tumor prevention.

The present invention relates to novel application of a protease tablysin in medicine. The protease tablysin can obviously inhibit adhesion and proliferation effects of human dermal microvascular endothelial cells (MVEC) and the angiogenesis in chicken embryo chorioallantoic membrane. According to research on three common tumor models, the protease tablysin has good antitumor effects, especially for liver cancer, lung cancer and gastric cancer, and can be used in preparation of antineoplastic medicine.

The invention belongs to the technical field of tumor treatment, and particularly relates to a synthetic peptide NK3R-A2 based on an NK3 receptor. The synthetic peptide NK3R-A2 based on the NK3 receptor has the tumor angiogenesis resisting effect. The molecular weight of the synthetic peptide is 1536.70 Da, and the specific sequence of the synthetic peptide is CNGRCGGDFF(MeF)GLM-NH2. The synthetic peptide is prepared through an Fmoc solid-phase polypeptide synthesis method and can be used for preparing anti-tumor preparations. Through in-vitro cell viability experiments, cell scratch experiments and cell migration experiments, the angiogenesis resisting effect of the synthetic peptide is verified; meanwhile, through further chicken chorioallantoic membrane carrier experiments and euangiotic mice S180 transplantation tumor model experiments on the synthetic peptide, it is verified that the synthetic peptide is obvious in tumor inhibiting effect, has the tumor angiogenesis resisting effect and has no obvious toxic or side effect; the synthetic peptide has good medical application prospects and also provides a targeting sequence with great potential for tumor treatment.

The invention discloses a blood vessel holder for chick embryo blood collection. The blood vessel holder comprises a blood vessel holding block and a supporting rod, the blood vessel holding block comprises a puncture portion, a holding portion and a mounting portion which are sequentially connected, a plurality of arc-shaped grooves for holding blood vessels are formed in the holding portion, theextending direction of the arc-shaped grooves is perpendicular to the length extending direction of the blood vessel holding block, and the mounting portion of the blood vessel holding block is fixedly connected with the supporting rod. According to the blood vessel holder, the puncture portion pierces a chorioallantoic membrane, the arc-shaped grooves of the holding portion are positioned belowthe blood vessels, the supporting rod is lifted to clamp the blood vessels into the arc-shaped grooves, so that the blood vessels are positioned and prevented from sliding when a needle draws blood bypuncture, blood drawing success rate is ensured, and operation efficiency is improved.

The invention relates to the technical field of bio-engineering and pharmaceuticals, in particular to a process for producing targeting anti-tumor antiangiogenesis fusion protein RT-X in colibacillus as well as a separation and purification method for the fusion protein RT-X. The fusion protein RT-X is soluble, has biological activity, and comprises RGD and Maspin protein. Through the adoption of the expressed and purified antiangiogenesis agent RT-X with the biological activity, endotheliocyte can be inhibited from migration according to specificity and dose dependence, and an effect for inhibiting angiogenesis is realized in a chicken embryo chorioallantoic membrane animal experiment. The process and the separation and purification method are applicable to research in bio-engineering pharmacy and gene engineering, biochemistry, molecular biology and other fields.

The invention discloses a tuna ground meat polypeptide angiogenesis inhibiting factor as well as a preparation method and an application thereof. The amino acid sequence of the polypeptide angiogenesis inhibiting factor is Trp-Val-Thr-Gly-Ile (WVTGI), and ESI / MS (electrospray ionization / mass spectrometry) detection shows that a molecular ion peak (m / z) is 575.42Da ([M+H]+). The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for effectively inhibiting angiogenesis of chorioallantoic membrane (CAM) of chicken embryo and multiplication of prostate cancer cell DU-145. The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for preparing a medicine used for inhibiting angiogenesis and preventing and treating prostate cancer.

The present invention belongs to the field of life sciences, and in particular to a preparation method of tissue engineering vascularization model from chicken embryo chorioallantoic membrane. After hatching of fertilized eggs, a culture system outside or inside shell is prepared; and when the allantoic membrane is mature, and has a large number of capillaries, an implant is placed next to the vessel for re-hatching. The chicken embryo chorioallantoic membrane model used by the method has rapid growth of blood vessels, and meets the blood vessel growth requirement of the implant; the model in a stationary state is easy for observation and experiment; and the most important thing is that the model does not have immunogenicity, can complete implantation of biological scaffold or living cells from all kinds of sources. The method has the advantages of easily obtained materials, low cost, easy operation, and no ethical limit of animal experiments.

The invention provides a preparation method for avian reovirus virus water-in-oil-in-water type inactivated vaccines. The preparation method comprises the following steps: preparation of chicken embryo fibroblast, cultivation of viruses, inactivation of the viruses, emulsification of the vaccines, and the like. Compared with the prior art, locally separated avian reoviruses are adopted, so that the immunogenicity is good, and the relatively high protection rate is adopted; the chicken embryo fibroblast is adopted to replace the utilization of a chicken embryo chorioallantoic membrane in a inoculation method for breeding the avian reoviruses, so that the vaccine preparation cost is saved, the mass discrepancy between each batch is lowered, the yield is high, and the viral titer and the vaccine quality are improved; the vaccines are subjected to emulsification by using a homogenizer, the prepared vaccines are water-in-oil-in-water type; compared with traditional water-in-oil type vaccines, the vaccines provided by the invention have low viscosity, small granularity, and easy absorption; surface active agent compositions are not required to be added in the preparation method by selecting and using an adjuvant, so that the prepared vaccines have good safety, and avoid side effects caused by difficult absorption of a traditional oil adjuvant.

The invention belongs to the technical field of tumor treatment, and particularly relates to a synthetic peptide NK3R-A1 based on an NK3 receptor. The synthetic peptide NK3R-A1 based on the NK3 receptor has the tumor angiogenesis resisting effect. The molecular weight of the synthetic peptide is 1330.46, and the specific sequence of the synthetic peptide is NGRGGDFF(MeF)GLM-NH2. The synthetic peptide is prepared through an Fmoc solid-phase polypeptide synthesis method and can be used for preparing anti-tumor preparations. Through in-vitro cell viability experiments, cell scratch experiments and cell migration experiments, the angiogenesis resisting effect of the synthetic peptide is verified; meanwhile, through further chicken chorioallantoic membrane carrier experiments and euangiotic mice S180 transplantation tumor model experiments on the synthetic peptide, it is verified that the synthetic peptide is obvious in tumor inhibiting effect, has the tumor angiogenesis resisting effect and has no obvious toxic or side effect; the synthetic peptide has good medical application prospects and also provides a targeting sequence with great potential for tumor treatment.

A method or composition including 6-aminopyridin-3-ol derivatives or pharmaceutically acceptable salts thereof for treating a disease caused by angiogenesis, the 6-aminopyridin-3-ol derivatives represented by Formula 1 or the pharmaceutically acceptable salts thereof have excellent neoangiogenesis inhibition effects in the chorioallantoic membrane model, and are suitable for use as a drug for the treatment of disease caused by angiogenesis.

The invention relates to the field of A61K8 / 60, in particular to a moisturizing composition as well as a preparation method and application thereof, and the moisturizing composition is prepared from the following raw materials: an aquatic algae extract, a pyrrolidinyl derivative, a humectant and a solvent. The composition prepared by the invention is added with the unique hydrolyzed red algae extract and sodium pyrrolidone carboxylate, the moisturizing property is good, the moisturizing rate within 2 hours can reach 116.30%, the long-acting water locking effect of the composition can be improved through compounding of the moisturizing agent, the moisturizing rate after 8 hours can reach 58.92%, and the composition is non-irritant to human skin through chick embryo chorioallantoic membrane tests, and has a good moisturizing effect. And the safety is high.