48 results about "Chorioallantoic membrane" patented technology

A method or composition including 6-aminopyridin-3-ol derivatives or pharmaceutically acceptable salts thereof for treating a disease caused by angiogenesis, the 6-aminopyridin-3-ol derivatives represented by Formula 1 or the pharmaceutically acceptable salts thereof have excellent neoangiogenesis inhibition effects in the chorioallantoic membrane model, and are suitable for use as a drug for the treatment of disease caused by angiogenesis.

The present invention relates to uses of a Sphyrna lewini cartilage angiogenesis inhibitory factor, namely, the Sphyrna lewini cartilage angiogenesis inhibitory factor is used to prepare health foods or functional additives for angiogenesis inhibition and tumor prevention. The amino acid sequence and ESI-MS detected molecular weight of the angiogenesis inhibitory factor is Pro-Asp-Tyr-Lys-Phe-Lys and 796.90 Da, respectively. The angiogenesis inhibitor factor can effectively inhibit angiogenesis in chick embryo chorioallantoic membrane, and significantly inhibits the expressions of three angiogenic factors including vascular endothelial growth factor, basic fibroblast growth factor and platelet derived growth factor in lung cancer tissues of mice bearing Lewis lung cancer, so that the angiogenesis inhibitor factor can be used to prepare health foods or functional additives for angiogenesis inhibition and tumor prevention.

The invention discloses a tuna ground meat polypeptide angiogenesis inhibiting factor as well as a preparation method and an application thereof. The amino acid sequence of the polypeptide angiogenesis inhibiting factor is Trp-Val-Thr-Gly-Ile (WVTGI), and ESI / MS (electrospray ionization / mass spectrometry) detection shows that a molecular ion peak (m / z) is 575.42Da ([M+H]+). The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for effectively inhibiting angiogenesis of chorioallantoic membrane (CAM) of chicken embryo and multiplication of prostate cancer cell DU-145. The tuna ground meat polypeptide angiogenesis inhibiting factor Trp-Val-Thr-Gly-Ile (WVTGI) can be used for preparing a medicine used for inhibiting angiogenesis and preventing and treating prostate cancer.

The invention provides a preparation method for avian reovirus virus water-in-oil-in-water type inactivated vaccines. The preparation method comprises the following steps: preparation of chicken embryo fibroblast, cultivation of viruses, inactivation of the viruses, emulsification of the vaccines, and the like. Compared with the prior art, locally separated avian reoviruses are adopted, so that the immunogenicity is good, and the relatively high protection rate is adopted; the chicken embryo fibroblast is adopted to replace the utilization of a chicken embryo chorioallantoic membrane in a inoculation method for breeding the avian reoviruses, so that the vaccine preparation cost is saved, the mass discrepancy between each batch is lowered, the yield is high, and the viral titer and the vaccine quality are improved; the vaccines are subjected to emulsification by using a homogenizer, the prepared vaccines are water-in-oil-in-water type; compared with traditional water-in-oil type vaccines, the vaccines provided by the invention have low viscosity, small granularity, and easy absorption; surface active agent compositions are not required to be added in the preparation method by selecting and using an adjuvant, so that the prepared vaccines have good safety, and avoid side effects caused by difficult absorption of a traditional oil adjuvant.

The invention belongs to the technical field of tumor treatment, and particularly relates to a synthetic peptide NK3R-A1 based on an NK3 receptor. The synthetic peptide NK3R-A1 based on the NK3 receptor has the tumor angiogenesis resisting effect. The molecular weight of the synthetic peptide is 1330.46, and the specific sequence of the synthetic peptide is NGRGGDFF(MeF)GLM-NH2. The synthetic peptide is prepared through an Fmoc solid-phase polypeptide synthesis method and can be used for preparing anti-tumor preparations. Through in-vitro cell viability experiments, cell scratch experiments and cell migration experiments, the angiogenesis resisting effect of the synthetic peptide is verified; meanwhile, through further chicken chorioallantoic membrane carrier experiments and euangiotic mice S180 transplantation tumor model experiments on the synthetic peptide, it is verified that the synthetic peptide is obvious in tumor inhibiting effect, has the tumor angiogenesis resisting effect and has no obvious toxic or side effect; the synthetic peptide has good medical application prospects and also provides a targeting sequence with great potential for tumor treatment.