12 results about "Chorioallantoic membrane" patented technology

The invention discloses a combined evaluation method for in-vitro and in-vivo red fading and relieving effects of cosmetic raw materials, which comprises the following steps: in-vitro model evaluation: inducing a human immortalized umbilical vein endothelial cell model by adopting TNF-alpha, and detecting the secretion amount of a prostacyclin I2 factor to obtain a score of an in-vitro model; in-vivo model evaluation: adopting a chick embryo chorioallantoic membrane model, and obtaining a score of the in-vivo model according to the degree of vasoconstriction caused by the to-be-detected raw material sample acting on the chick embryo chorioallantoic membrane; and comprehensive evaluation: based on the score of the in-vitro model and the score of the in-vivo model, comprehensively evaluating the red fading and relieving efficacy of the raw material sample to be tested. According to the technical scheme, the defect that in the prior art, no cosmetic raw material soothing efficacy evaluation method for the vascular reactivity increasing mechanism exists is overcome, efficacy evaluation is more accurate, the method has the advantages of being comprehensive, short in test period, easy to operate, economical and convenient, and an effective reference basis is provided for screening and compounding schemes of red fading and soothing raw materials.

To provide avian model allowing the expansion of human or animal circulating tumor cells (CTCs), and to provide uses thereof for monitoring and determining the sensitivity of patients or animals suffering from cancer to one or more therapeutic agents and for screening novel therapeutic agents for the treatment of cancer.SOLUTION: Provided is an avian embryo-containing egg model comprising human or animal CTCs isolated from a sample of a patient or animal suffering from cancer, the CTCs being implanted at the level of the chorioallantoic membrane (CAM) of an embryo-containing egg, and the embryo-containing egg being at a developmental stage corresponding to the formation of the CAM and corresponding to at least 8 days of chicken development at the time of implantation.SELECTED DRAWING: None

The utility model discloses a cosmetic eye irritation detection and analysis device which comprises a base, and the right side of the top of the base is fixedly connected with an analysis device main body. Through the arrangement of the groove, the analysis device is started, the start button is pressed down to start the automatic sample dropping pipettor, after the automatic sample dropping pipettor drips a sample to a chick embryo chorioallantoic membrane in the groove under the automatic sample dropping pipettor, the stepping motor is automatically started and drives the rotating base plate to rotate by 36 degrees, and the chick embryo chorioallantoic membrane is automatically dropped into the chick embryo chorioallantoic membrane in the groove under the automatic sample dropping pipettor. The chick embryo chorioallantoic membrane in the next groove is moved to the position under the automatic sample dropwise adding pipettor for dropwise adding the sample, and a pause button is controllably pressed until the ten chick embryo chorioallantoic membranes are dropwise added completely, so that the stepping motor and the automatic sample dropwise adding pipettor stop working, and then the chick embryo chorioallantoic membranes can be covered with a preservative film for constant-temperature culture; the dripping process is automatically and quickly completed, so that the analysis efficiency is improved, the use of a user is facilitated, and the practicability of the analysis device is improved.

The invention discloses an anti-sugar moisturizing brightening mask composition without irritation to eyes. The anti-sugar moisturizing brightening mask composition comprises the following raw materials in percentage by weight: 92.99% of water; 2% of glycerin; 0.2% of Giga Moist PM-II hydrolyzed sclerotium gum, and 0.2% of Giga Moist PM-II hydrolyzed sclerotium gum; 0.03% of beta-glucan; 3% of 1, 2-propylene glycol; 0.1% of cosmetic grade sodium hyaluronate; 1% of PPG-24-glyceryl polyether-24; 0.1% of Xinhaidew acetyl chitosamine; 0.6% of Kesen K708; 0.05% of essence; according to the composition disclosed by the invention, the irritation test is carried out by using a cosmetic eye irritation-chick embryo chorioallantoic membrane vascular test method CAMVA method, and the detection method not only shortens the component irritation screening detection period of the composition, but also reduces the cost; meanwhile, a chick embryo chorioallantoic membrane blood vessel test method meets animal protection ethical requirements, the prepared composition finished product contains components capable of resisting saccharification and brightening skin, can be used by various skin types, can effectively reduce fine wrinkles and wrinkles and brighten the skin color, and finally has the effects of resisting wrinkles, preserving moisture and reducing dull skin for sensitive skin consumers.

The invention provides a method for evaluating eye irritation of cosmetics, and relates to the technical field of detection. The method comprises the following steps: firstly, acquiring a first image of a chick embryo chorioallantoic membrane before contacting a to-be-detected sample; removing the to-be-detected sample contact film after a preset time to obtain a second image; performing color channel separation on the two images, and selecting a green channel image to perform gray value analysis to obtain a first gray value and a second gray value; and finally evaluating the eye irritation level of the sample to be detected according to the gray value difference. According to the method disclosed by the invention, quantitative evaluation on eye irritation is realized by utilizing the optical characteristic of chick embryo blood vessels to green light, the subjective defect that a traditional chick embryo chorioallantoic membrane test depends on visual inspection is overcome, the objectivity, accuracy and repeatability of a detection result are remarkably improved, the operation is simple and convenient, and animal ethical dispute is avoided.

The present invention relates to an injection and live detection device for chicken embryo model for VDAs efficacy research, which comprises a container for receiving chicken embryo, an injection mechanism for injecting chorioallantoic membrane blood vessels, and a live detection mechanism for monitoring changes in blood perfusion, wherein the container is provided with a downwardly concave receiving groove, a light-transmitting hole penetrating the container is provided below the container, the light-transmitting hole is communicated with the receiving groove, the injection mechanism comprises a handleless needle, an extension tube, and a micro-injection pump, one end of the extension tube is used as a first connection end, and the other end is used as a second connection end, the second connection end is connected to the syringe in the micro-injection pump, and the first connection end is connected to the handleless needle and forms a clearance fit. The present invention has the following advantages and effects: it can realize micro, accurate, and slow administration of drugs to the chorioallantoic membrane of chicken embryos, and can detect the efficacy of VDAs on vascular changes and blood perfusion in real time and live.

The invention discloses eye drops for treating vascular hyperplasia of eyes and a preparation method of the eye drops, and belongs to the technical field of biological pharmacy. The eye drops comprise a recombinant human angiostatin protein, an endostatin protein and a pharmaceutically acceptable carrier, wherein the recombinant human angiostatin protein and the endostatin protein are used for preparing the eye drops. Experiments show that the eye drops show remarkable vascular inhibition activity in a chick embryo chorioallantoic membrane model and a diabetic rat retina hyperplasia model, and the two proteins are combined for use to achieve a synergistic effect; the invention provides a safe and effective new way for local treatment of ocular vascular hyperplasia diseases.

The invention provides a bovine nodular dermatosis virus passage attenuating method and application thereof.The method comprises the steps that a bovine nodular dermatosis virus LSDV NM2020 strain serves as a parent strain, 25 generations of continuous passage is conducted through 9-day-old SPF chick embryo chorioallantoic membrane, 30 generations of continuous passage is conducted in Vero E6 cells in a gradual cooling mode, and finally the attenuated strain is obtained through plaque cloning and purification; the strain is named as an LSDV-Z strain. The low virulent strain shows good safety in cattle while keeping the growth titer close to that of a parent strain, does not cause clinical symptoms and does not have an external detoxification phenomenon. A virus attack protection test proves that the LSDV-Z strain can provide 100% immune protection for virulent virus attack. The method disclosed by the invention has the advantages of short attenuation period, high efficiency, no need of transgenic operation and the like, and the obtained LSDV-Z strain can be used as a safe and effective candidate vaccine strain and is used for preventing and controlling bovine nodular dermatosis.

The application discloses a combined evaluation method for the in-vitro and in-vivo redness-reducing soothing effect of a cosmetic raw material, which comprises in-vitro model evaluation, adoption of a human immortalized umbilical vein endothelial cell model induced by TNF-alpha, acquisition of the in-vitro model score by detecting the secretion amount of prostacyclin I2 factor, in-vivo model evaluation, adoption of a chicken embryo chorioallantoic membrane model, acquisition of the in-vivo model score by the degree of blood vessel contraction caused by the action of the sample to be tested on the chicken embryo chorioallantoic membrane, and comprehensive evaluation of the redness-reducing soothing effect of the sample to be tested based on the in-vitro model score and the in-vivo model score. The technical scheme of the application makes up for the defect of the prior art that there is no cosmetic raw material soothing effect evaluation method for the mechanism of increased vascular reactivity, the effect evaluation is more accurate, and the method has the advantages of comprehensiveness, short test cycle, simple operation, economy and convenience, and provides an effective reference for the screening and compounding scheme of redness-reducing soothing raw materials.

To provide a method capable of evaluating the multipotency of mammalian cells without using experimental mammals, such as immunodeficient mice, that can reproduce results from teratoma formation tests employing the experimental mammals, that has better cost effectiveness and time effectiveness than cases employing the experimental mammals, and that consists of relatively simple procedures.SOLUTION: A method of evaluating the multipotency of mammalian cells comprises the following steps: (a) transferring the mammalian cells onto a chorioallantoic membrane of an embryonated egg, and then culturing the embryonated egg; and (b) evaluating the multipotency of the mammalian cells using, as an indicator, teratoma formation in the embryonated egg cultured in the step (a).SELECTED DRAWING: None

The invention belongs to the technical field of biological medicine, and discloses application of a chick embryo allantoic membrane model in lung adenocarcinoma drug resistance mechanism research. The chick embryo allantoic membrane model is obtained by hatching fertilized white-shell eggs in a bird incubator at 37.5 DEG C and 65% humidity. After cancer cells are transplanted to Y-shaped blood vessels of the chorioallantoic membrane, H2228 cells continuously expressing Venus fluorescent protein are transplanted to the chorioallantoic membrane so as to confirm the implantation condition. Before and after medicine injection, all organs of the cut tumor and embryo are shot under fluorescence, and the fluorescence intensity of all the organs is quantitatively analyzed through a fluorescein filter. According to the invention, a chick embryo allantoic membrane model is used for verifying the cancer inhibition effect of Chb-M'in ALK mutant lung adenocarcinoma cells for the first time, the experiment cost is relatively low, and the experiment period is relatively short.

The invention discloses a method for constructing an evaluation model for removing redness based on cytokine-stimulated chicken embryo angiogenesis: determining the safe concentration of the test substance; dividing the cell culture into a model control group, a negative control group, a positive control group and a test substance group, collecting the supernatant after the incubation; detecting cytokines in the supernatant; selecting fertilized chicken embryos in the rapid growth period of blood vessels, dividing them into a test substance group, a negative control group, a positive control group and a model control group, and recording the distribution of chorioallantoic blood vessels; adding the supernatant collected from the corresponding groups to the chorioallantoic membrane, and recording the distribution of chorioallantoic blood vessels after incubation; counting the bifurcation of blood vessels, calculating the angiogenesis rate and the vascular inhibition rate, and evaluating the effect of removing redness of the test substance. The test substance conditions of the invention are low, and cells are used as the experimental system, with high sensitivity; the experimental cycle is short, and a large number of samples can be quickly detected. Using cells and chicken embryos as experimental models is simple to operate, and the results are intuitive, which is an ideal model for predicting the effect of removing redness.