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Method for detecting and evaluating conjunctiva irritation and mucous membrane irritation by utilizing chick embryo blood vascular system

A vascular system and irritation technology, which is applied in the field of detection and evaluation of conjunctival irritation and mucous membrane irritation by using chicken embryo vascular system, can solve problems such as insoluble, high cost, and health hazards of volunteers, and achieve a high degree of standardization The system is simple and the detection method is fast

Inactive Publication Date: 2017-10-20
秦瑶 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

437 is a bovine corneal turbidity and permeability test for eye irritation. The waste material from the slaughterhouse is used as the test system to detect the eye irritation of chemicals and products. Its advantage is that it is suitable for the detection of almost all raw materials and products. The disadvantage is that domestic materials Difficult source, primarily assesses corneal damage, cannot detect conjunctival irritation
460 is a method of single-layer cell culture, which is mainly suitable for the detection of chemicals such as surfactants. Its disadvantage is that it is not suitable for the detection of insoluble and colored chemicals, nor is it suitable for testing formulations and extracts with complex components. , formulations and finished products
492 is a method of tissue engineering cornea, which requires the use of commercialized artificial cornea models. At present, similar products cannot be produced in China, nor can they be effectively imported. Secondly, artificial cornea models are expensive and not suitable for a large number of daily tests.
The results of human experiments are the most authentic, but the cost is too high, there are health hazards to volunteers, and there are certain ethical problems

Method used

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  • Method for detecting and evaluating conjunctiva irritation and mucous membrane irritation by utilizing chick embryo blood vascular system
  • Method for detecting and evaluating conjunctiva irritation and mucous membrane irritation by utilizing chick embryo blood vascular system
  • Method for detecting and evaluating conjunctiva irritation and mucous membrane irritation by utilizing chick embryo blood vascular system

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0152] Embodiment 1 utilizes chicken embryo vasculature to detect and evaluate the method for sulfacetamide sodium conjunctival irritation

[0153] 1. Preparation of natural chorioallantoic membrane

[0154] Purchase 7d-year-old chicken embryos, store them in egg racks with the air chamber facing up, and transfer them to the laboratory, inspect them as eggs, and discard inactive and defective chicken embryos. The chicken embryos are incubated at an incubation temperature of 37.5°C±0.5°C and a relative humidity of 60%-70% until they are 10 days old. At the age of 10 days, check the chicken embryo according to the egg, mark the position of the air cell on the surface of the eggshell; peel off the eggshell part with dental serrated curved tweezers, expose the white egg film, add 1-2mL of 0.9% NaCl solution to make the egg film wet, and then The solution was decanted and the inner membrane was carefully removed with forceps, exposing the native chorioallantoic membrane.

[0155]...

Embodiment 2

[0170] Example 2 The method for detecting and evaluating eye cream conjunctival irritation by using chicken embryo vascular system

[0171] 1. Preparation of artificial chorioallantoic membrane

[0172] The purchased SPF grade fertilized chicken embryos with an embryonic age of 0 days were incubated in a constant temperature incubator with a temperature of 37°C ± 1°C and a relative humidity of 65% ± 5%. On day 3, place the embryos horizontally. On the 4th day, check the chicken embryos against the light, and discard the chicken embryos that are not fertilized or underdeveloped. For normally developing chicken embryos, a small hole is opened from the non-air cell side of the egg, the dust is wiped off with an alcohol cotton ball, and 2.5-3mL egg white is drawn out from the small hole with a syringe to form an artificial chorioallantoic membrane. The pores were sealed with paraffin. Then open a small rectangular window with a side length of 0.8-1 cm above the top of the egg. ...

Embodiment 3

[0187] Example 3 Method for detecting and evaluating the conjunctival irritation of shampoo using chicken embryo vasculature

[0188] 1. Preparation of artificial chorioallantoic membrane

[0189] The purchased SPF grade fertilized chicken embryos with an embryonic age of 0 days were incubated in a constant temperature incubator with a temperature of 37°C ± 1°C and a relative humidity of 65% ± 5%. On day 3, place the embryos horizontally. On the 4th day, check the chicken embryos against the light, and discard the chicken embryos that are not fertilized or underdeveloped. For normally developing chicken embryos, a small hole is opened from the non-air cell side of the egg, the dust is wiped off with an alcohol cotton ball, and 2.5-3mL egg white is drawn out from the small hole with a syringe to form an artificial chorioallantoic membrane. The pores were sealed with paraffin. Then open a small rectangular window with a side length of 0.8-1 cm above the top of the egg. The sma...

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Abstract

The invention discloses a method for detecting and evaluating conjunctival irritation and mucous membrane irritation by using chicken embryo vascular system, comprising the following steps: (1) preparation of chorioallantoic membrane; (2) conjunctival irritation test; (3) sticking mold Stimulation test; (4) prediction model and classification, the present invention uses the standardized chicken embryo as the test system, and its vascular system at different stages of development is similar to the human eye conjunctiva and oral submucosa tissue, and the chicken embryo has a high degree of standardization, stable source and batch The difference between blood vessels is small, the vascular system has biological activity and function, the test system is simple and cheap, no special equipment is required, and the detection method is fast, sensitive and universal. The invention can detect and evaluate the conjunctival irritation and mucous membrane irritation of the substance to be tested from both qualitative and quantitative aspects. The method of the present invention can also replace living animals and human volunteers, and be directly used for the detection of conjunctival irritation and mucosal irritation of substances such as chemicals or cosmetics, and rapid screening, classification and identification thereof.

Description

technical field [0001] The invention belongs to the technical field of conjunctival irritation and mucosal irritation, and in particular relates to a method for detecting and evaluating conjunctival irritation and mucosal irritation by using chicken embryo vascular system. Background technique [0002] With the improvement of living standards, more and more people start to use personal care and cleaning products, the former such as skin and eye products, the latter such as oral cleaning and care products. Most consumers are very concerned about their safety issues during the use of these products, including the irritation of the conjunctiva and cornea caused by unintentional contact with the eyes after using the products; and the irritation of oral care products to the oral mucosa and gums; Irritation of the vaginal mucosa, etc. Therefore, regulatory requirements for personal care and cleaning products must test products for health hazards. At the same time, during the res...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/50
CPCG01N33/5008G01N33/5088
Inventor 秦瑶程树军陈志杰
Owner 秦瑶
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