37results about How to "Has a biological function" patented technology

The invention discloses a novel method for reducing and preparing functionalized nano-silver based on polyphenol. The preparation process is as follows: 1), stock solutions of all reaction liquids are prepared, and the stock solutions comprise a silver-bearing predecessor stock solution, a polyphenol compound stock solution and an alkaline stock solution; 2), a polyphenol compound solution is prepared: a certain amount of the polyphenol compound stock solution is taken and added into ultrapure water, and the stock solution and the ultrapure water are mixed uniformly; 3), the pH value and the temperature of the polyphenol compound solution are adjusted: a proper amount of the alkaline stock solution is taken and added into the polyphenol compound solution prepared in the step 2), the pH value of the mixed solution is adjusted, the mixed solution is placed and kept out of the sun, and the temperature of the mixed solution is adjusted; and 4), a nano-silver solution is generated: a certain amount of the silver-bearing predecessor stock solution is added into the mixed solution with adjusted pH value and temperature in the step 3) and is kept out of the sun, and reaction is performed for a certain time. The operation method is simple, the synthesis speed is high, the cost is low, the biocompatibility is high, the method is pollution-free, the sizes of the synthesized nano-silver particles are small, the monodispersity is good, and the nano-silver also has the performance of a polyphenol compound.

The invention discloses a nucleic acid aptamer delivery carrier, a pharmaceutical composition containing the carrier, and a preparation method and application of the carrier and pharmaceutical composition. The nucleic acid aptamer delivery carrier comprises a nano vesicle taking an erythrocyte membrane as a skeleton, and the nano vesicle is loaded with a cholesterol-modified nucleic acid aptamer.The obtained drug-loaded targeting nano vesicle has the specificity to tumor cells, can silence the expression of P-glycoprotein in drug-resistant tumor cells and reverse the drug resistance of the drug-resistant tumor cells, and at the same time realizes the effective killing of the drug-resistant tumor cells at a relatively low adriamycin concentration.

The invention relates to a method for preparing a constructed body from a biobrick containing endothelial cells, belonging to the technical fields of biology, regenerative medicine, biological printing (e.g., 3D biological printing), tissue engineering, etc. Particularly, the invention relates to a biobrick applicable to biological printing (e.g., 3D biological printing) and tissue engineering, a composition (e.g., biological ink) including the biobrick, a preparation method and application of the biobrick and the constructed body (e.g., a three-dimensional constructed body) including the biobrick or constructed by using the biobrick / biological ink. The biobrick comprises cells, a core wrapping cells (e.g., endothelial cells), and a shell encapsulating the core.

The invention discloses a detachable talus prosthesis which comprises a talonavicular subtalar joint assembly and a tibiotalar joint assembly. On one hand, the detachable talus prosthesis is formed by combining the talonavicular subtalar joint assembly and the tibiotalar joint assembly and is easy to manufacture; on the other hand, the subtalar joint surface and talonavicular joint surface are of porous structures, and the detachable talus prosthesis has a biological function and facilitate rapid fusion and growth of prosthesis and calcaneus and scaphoid bone.

The invention relates to a method for inducing in vitro directional differentiation of stem cells by cell co-culture. An annular gasket is arranged on a bottom of a culture dish. Firstly, stem cells are inoculated in an annular zone and are cultured in an incubator. When the stem cells attach a culture dish wall, a mixed solution containing agarose and sodium alginate is added to the annular zone. When the mixed solution is solidified, a calcium chloride solution is added for a carrying out a calcification reaction and a chitosan is added for carrying out a reaction to form an agarose / sodium alginate / chitosan flat composite gel layer on the stem cells. Inductive cells are inoculated on a surface of the composite gel. By means of the co-culture of the inductive cells and the stem cells, interactions among in vivo cells and between cells and soluble factors can be simulated. By means of the polysaccharide-based agarose / sodium alginate / chitosan flat composite gel, an in vivo extracellular matrix can be simulated. The directional differentiation of the stem cells can be adjusted and controlled through rigidity and thickness of the composite gel. Meanwhile, isolated co-culture of the stem cells and the inductive cells can be achieved so that differentiated cells are easy to collect. The method has an important effect in application of regenerative medicine.

The invention discloses a pharmaceutical composition for control of diabetes and complications thereof. The pharmaceutical composition comprises: sea cucumber polypeptide, alginate oligosaccharide, a kudzu vine root extract, a common duckweed extract, a radix trichosanthis extract, an extract of peach kernel and safflower, a ginkgo leaf extract, hirudin and nattokinase. The pharmaceutical composition provided by the invention can treat insulin resistant diabetes, the effective components are compounded to achieve better prevention and treatment effect on diabetes than single component, and the pharmaceutical composition also can prevent and treat some chronic complications associated with blood glucose rise. The pharmaceutical composition provided by the invention has the advantages of simple production process, low cost, high benefit, and obvious economic and social benefits.

The invention discloses a talus prosthesis including a subtalar articular surface and a talonavicular articular surface. The subtalar articular surface and / or the talonavicular articular surface are of porous structures. The talus prosthesis has biological functions, facilitates quick fusion and growth of the prosthesis and a calcaneus and a scaphoid, and thus has great medical value.

The invention relates to the technical fields of biology, regenerative medicine, biological printing (e.g., 3D biological printing), tissue engineering, etc. Particularly, the invention relates to a biobrick applicable to biological printing (e.g., 3D biological printing) and tissue engineering, a composition (e.g., biological ink) including the biobrick, a preparation method and application of the biobrick and a constructed body (e.g., a three-dimensional constructed body) including the biobrick or constructed by using the biobrick / biological ink. The biobrick comprises cells, a core wrapping cells (e.g., endothelial cells), and a shell encapsulating the core.

The invention discloses a food-borne selenium-rich antioxidative peptide and a preparation method thereof. A by-product, namely selenium-rich rice bran obtained through selenium-rich brown rice processing serves as a raw material and is preprocessed, then, selenoprotein is extracted, the selenoprotein is subjected to composite enzymatic hydrolysis, separation and purification and activity checking, and then the selenium-rich peptide with antioxidant activity is obtained. The method has the advantages that safety is high, conditions are mild, a process is simple, and product activity is high. When applied to foods and especially to functional foods, the obtained selenium-rich antioxidative peptide can play a role of antioxidation of an active peptide, can also improve the intake of selenium in food of people and has multiple beneficial effects. In addition, the selenium-rich rice bran is the by-product produced through selenium-rich brown rice processing, and the selenium-rich antioxidative peptide prepared from the selenium-rich rice bran can effectively improve the resource utilization rate and increase the added value of the product.

The invention relates to a polyselenoamino acid amphiphilic block copolymer as well as a preparation method and application thereof, and the polyselenoamino acid amphiphilic block copolymer has a structure as shown in a formula (I) which is described in the specification. The polyselenoamino acid amphiphilic block copolymer nano-micelle can be self-assembled to form nano-drug carriers (such as nano spherical micelles, nano rodlike micelles and nano vesicles) in different forms in different environments. The nano-drug carrier can be used for research and development of various drugs, can load drug molecules to prepare a sustained-release drug delivery system, and can be administrated in various modes, and the entrapped drugs can be released from a delivery system in a sustained-release modeaccording to needs, so that the administration frequency is reduced, the treatment effect is improved, and the toxic and side effects of the drugs are reduced.

The invention discloses an individual all-talus prosthesis having biological functions, which can prepare the talus prosthesis having individualizing and biological functions and a making method of the individual all-talus prosthesis. The making method of the individual all-talus prosthesis having biological functions comprises the following steps of obtaining raw data of talus of damaged sides and healthy sides; through image / backward design, obtaining a talus prosthesis model, and dividing the talus prosthesis model into a talonavicular hypozygal assembly and a tibiotalar joint facet assembly; performing 3D printing on the talonavicular hypozygal assembly, and designing a casting mould of the tibiotalar joint facet assembly; and through a 3D printing technique, printing the mould, through the mould, performing casting to obtain the tibiotalar joint facet assembly, and finally performing combination so as to obtain the astragalus prosthesis. The prosthesis comprises talonavicular hypozygal assembly, the talonavicular joint assembly and the tibiotalar joint assembly. Through the individual all-talus prosthesis having biological functions and the making method of the individual all-talus prosthesis, the motion function of the ankle joint can be guaranteed not to be influenced, individualized preparation can be realized, and the making cost of the prosthesis can be reduced.

The invention discloses a method for detecting and evaluating conjunctival irritation and mucous membrane irritation by using chicken embryo vascular system, comprising the following steps: (1) preparation of chorioallantoic membrane; (2) conjunctival irritation test; (3) sticking mold Stimulation test; (4) prediction model and classification, the present invention uses the standardized chicken embryo as the test system, and its vascular system at different stages of development is similar to the human eye conjunctiva and oral submucosa tissue, and the chicken embryo has a high degree of standardization, stable source and batch The difference between blood vessels is small, the vascular system has biological activity and function, the test system is simple and cheap, no special equipment is required, and the detection method is fast, sensitive and universal. The invention can detect and evaluate the conjunctival irritation and mucous membrane irritation of the substance to be tested from both qualitative and quantitative aspects. The method of the present invention can also replace living animals and human volunteers, and be directly used for the detection of conjunctival irritation and mucosal irritation of substances such as chemicals or cosmetics, and rapid screening, classification and identification thereof.

The invention discloses an anti-CD20 antibody-monomethyl auristatin E (MMAE) conjugate, its preparation method and application. The anti-CD20 antibody-monomethyl auristatin E conjugate is formed by coupling of monomethyl auristatin E to the anti-CD20 antibody through a linking arm. Specifically, the light chain amino acid sequence of the anti-CD20 antibody is shown as SEQ ID No.1, and its heavy chain amino acid sequence is shown as SEQ ID No.2. The anti-CD20 antibody-monomethyl auristatin E conjugate can be applied in preparation of antitumor drugs. The conjugate provided in the invention has the biological functions of resisting both the CD20 antibody and MMAE, and under cooperation of the two, the anti-tumor effect can be significantly enhanced.

The invention discloses an anti-CD20 monoclonal antibody-aplysiatoxin conjugate as well as a preparation method and application thereof. The preparation method comprises the steps of dissolving the anti-CD20 monoclonal antibody into a buffer solution, adding trichloroethyl phosphate to carry out reduction reaction; mixing restored anti-CD20 monoclonal antibody with aplysiatoxin having a connecting arm to carry out coupled reaction; and separating and purifying to obtain the anti-CD20 monoclonal antibody-aplysiatoxin conjugate after reaction is finished. Compared with the prior art, 8 aplysiatoxin molecules are coupled in the conjugate disclosed by the invention at most; the anti-CD20 monoclonal antibody-aplysiatoxin conjugate has the biological functions of the anti-CD20 monoclonal antibody and MMAE, has strong tumor targeting property, can kill CD20 positive tumor cells, and also reduces the toxic and side effects caused by independent dosing of the MMAE; and the anti-tumor effect is obviously reinforced.

The invention discloses an extraction method of nucleoprotein in animal cells. In the method, cell penetrating peptide, exogenous nucleoprotein and enhanced green fluorescent protein are utilized to jointly construct a recombinant nucleoprotein expression vector; recombinant nucleoprotein is mediated to express in nuclei; after the recombinant nucleoprotein is stably expressed in the cells, the cells are treated by using an operation solution; the cells are collected and crushed with ultrasonic wave; after the cells are centrifuged to remove precipitate, the supernatant fluid of the cells is collected, filtered and sterilized, and added into a cell culture solution; and 4 hours later, the extracted fluorescence labeled exogenous recombinant nucleoprotein enters the nuclei through cytoplasm and is positioned in the nuclei. According to the method, the extracted nucleoprotein can play functions of protein, so that morphological character of the cell changes; and results prove that the method can easily, quickly and efficiently extract the nucleoprotein with bioactivity in the cells.

The invention discloses a preparation method of yoghourt having the functions of assisting in reducing blood pressure and protecting the liver. The preparation method comprises the following steps of adding a mixture of 3-6g of casein hydrolysate rich in ACE restraint peptide and 1.5-6g of a purple sweet potato extract rich in anthocyan in a formula of raw materials for producing 1000g of yoghourt; and after uniformly mixing the raw materials, performing pasteurization, performing inoculation of lactic acid bacteria, and performing fermentation so as to obtain the fermented yoghourt having the functions of assisting in reducing blood pressure and protecting liver. According to the preparation method disclosed by the invention, the purple sweet potato extract rich in anthocyan and the casein hydrolysate rich in ACE activity restraint peptide are used as main functional raw materials for producing fermented milk products having the effects of assisting in reducing blood pressure and protecting the liver, and the used raw materials are food raw materials and have higher eating safety.

Collagen and hyaluronic acid or its sodium salt as main material are prepared into double layer composite material with compact layer and loose layer. The mass composition of the compact layer includes malonic acid swelling solution in 0.6-0.8 wt%; and the mass composition of the loose layer includes malonic acid swelling solution in 0.2-0.4 wt% and hyaluronic acid or its sodium salt in 5-20 wt% of dry collagen. The present invention has loose structure in one side and compact structure in the other side, and thus excellent biocompatibility capable of resisting the growth of fibroblast and other cell to the damaged part while promoting the regeneration and repairing of tissue in the damaged part. The double layer composite material has corresponding biodegrading function and may be degraded and absorbed without needing secondary operation to take out the implanted material. The present invention is suitable for repairing tissue damage.

The invention relates to a multifunctional nano-drug carrier, a preparation method thereof and a drug-loading composition, and belongs to the technical field of biomedical drug carriers and sustained-release drug carriers. The nano-drug carrier is formed by self-assembling a poly-seleno-amino acid amphiphilic block copolymer with a structure shown in the following formula, wherein n is more than or equal to 22 and less than or equal to 454, x is more than or equal to 2 and less than or equal to 50, y is more than or equal to 2 and less than or equal to 50, and n, x and y are integers. The nano-drug carrier provided by the invention can load drug molecules to prepare a sustained-release and controlled-release drug delivery system, and can be used for drug delivery in various modes, and theloaded drug can be released from the delivery system in a sustained-release and controlled-release mode according to needs, so that the drug delivery frequency is reduced, the treatment effect is improved, and the toxic and side effects of the drug are reduced. Compared with the prior art, the nano-drug carrier provided by the invention has the advantages of a common amino acid nano-drug carrier,has a plurality of biological functions of selenium, and is a novel multifunctional nano-drug carrier.

The invention provides an application of peanut citric acid transport protein gene AhFRDL1 in improving aluminum toxicity stress resistance of plants. The application comprises: transferring the peanut citric acid transport protein gene AhFRDL1 or a recombinant expression vector containing the peanut citric acid transport protein gene AhFRDL1 into plant cells, screening transgenic plants, and making the transgenic plants express the peanut citric acid transport protein gene so as to improve the aluminum toxicity stress resistance of the plants. In the invention, the fact that the peanut citric acid transport protein gene AhFRDL1 is gene for regulating and controlling secretion of citric acid in vitro of the plants and participates in the biological process of the aluminum toxicity stress resistance of plants is proved at first, and the peanut citric acid transport protein gene AhFRDL1 is used for improving the aluminum toxicity stress resistance of plants, which has long-term practical significance for further breeding aluminum toxicity resistant main crops on acid soil.

The invention discloses a method for constructing a cartilage patch stent based on polycaprolactone electrostatic spinning 3D printing and a device thereof. The method comprises the following steps: (1) designing an individualized 3D model of a stent according to characteristic parameters of the stent based on individual characteristics; (2) by utilizing an electrostatic spinning 3D printing technology and taking polycaprolactone as a raw material, printing the cartilage patch stent according to the individualized 3D model; (3) inducing the cartilage cells to be planted on the cartilage patchstent, amplifying the cartilage cells on the stent, secreting extracellular matrixes, and forming a cartilage cell-extracellular matrix-support complex. According to the preparation method, polycaprolactone is taken as the raw material, a technical means of combining electrostatic spinning and 3D printing is employed so as to construct the cartilage patch, and the prepared cartilage patch is widerin application range and excellent in biomechanical property.

The invention discloses an siRNA delivery carrier and a preparation method and application thereof. The siRNA delivery carrier comprises a nano vesicle and siRNA loaded in the nano vesicle, and the nano vesicle is prepared from an erythrocyte membrane. The invention further discloses an siRNA delivery carrier based on nucleic acid aptamer targeting, which comprises a nano vesicle, siRNA loaded in the nano vesicle and the cholesterol modified nucleic acid aptamer loaded on the surface of the nano vesicle. The nano vesicle is prepared from an erythrocyte membrane. In the siRNA delivery carrier based on nucleic acid aptamer targeting, which is prepared by the invention, the siRNA is loaded in the nano vesicle, the encapsulation efficiency is higher, and the siRNA delivery carrier is difficult to degrade.

The invention relates to the technical fields of biology, regenerative medicine, biological printing (e.g., 3D biological printing), tissue engineering, etc. Particularly, the invention relates to a biobrick applicable to biological printing (e.g., 3D biological printing) and tissue engineering, a composition (e.g., biological ink) including the biobrick, a preparation method and application of the biobrick and a constructed body (e.g., a three-dimensional constructed body) including the biobrick or constructed by using the biobrick / biological ink. The biobrick comprises cells, a core wrapping cells (e.g., endothelial cells), and a shell encapsulating the core.

The invention relates to ERG1 gene-deficient yeast engineering bacteria and a construction method thereof, wherein the ERG1 gene-deficient yeast engineering bacteria transforms yeast by a CRISPR / Cas9gene editing technology, an Erg1 gene sequence can be completely deleted, back mutation phenomena produced by yeast self-repair can be avoided, and engineering bacteria that can be used to verify other squalene-derived epoxidase genes can be obtained.

The invention relates to the technical fields of biology, regenerative medicine, biological printing (such as 3D biological printing), tissue engineering and the like. Particularly, the invention relates to a biological brick which can be used for biological printing (such as 3D biological printing) and tissue engineering, a composition (such as biological ink) containing the biological brick, a preparation method and application of the biological brick, and a structure (such as a three-dimensional structure) containing the biological brick or constructed by using the biological brick / biological ink, wherein the biological brick comprises cells, a core layer wrapping the cells (such as endothelial cells), and a shell layer packaging the core layer.

The invention discloses a preparation method of a double-protein co-emulsified Pickering emulsion, and belongs to the technical field of food processing. The food-grade Pickering emulsion is preparedfrom soybean protein isolate nanoparticles and lactoferrin nanoparticles, so that the food-grade Pickering emulsion is more stable in the day and has excellent anti-aggregation stability. The preparation method provided by the invention is of great significance to design and manufacture of a formula of the protein-stabilized Pickering emulsion and even development of soybean protein products withcertain unique functions. In addition, 7s and 11s proteins in the soybean protein used in the method have good surface activity characteristics, so that the Pickering emulsion prepared by the method has good stability. The bovine lactoferrin is high in isoelectric point and is positively charged under a neutral condition, so that the bovine lactoferrin can interact with other biological macromolecules to improve the stability of the Pickering emulsion.

The invention relates to a method for cell co-cultivation to induce stem cell directional differentiation in vitro. An annular spacer is installed at the bottom of the culture dish, stem cells are inoculated in the annular area, cultivated in an incubator, and then the agarose / Sodium alginate mixed solution is added to the annular area, after the solution is solidified, add CaCl 2 The solution is calcified, and chitosan solution is added to react to form a layer of agarose / sodium alginate / chitosan slab composite gel on the stem cells. Induced cells were seeded on the surface of the composite gel. The co-cultivation of induced cells and stem cells in the present invention can simulate the interaction between cells in vivo and between cells and soluble factors, and the polysaccharide-based agarose / sodium alginate / chitosan slab composite gel can simulate the extracellular matrix in vivo, Its stiffness and thickness can regulate the directional differentiation of stem cells, and at the same time realize the isolation and co-culture of stem cells and induced cells, making the differentiated cells easy to harvest, and will play an important role in the application of regenerative medicine.

The invention relates to a multifunctional nano-drug carrier of a targeted lactoferrin receptor, a preparation method of the multifunctional nano-drug carrier and a drug-loading composition comprising the multifunctional nano-drug carrier. The multifunctional nano-drug carrier is prepared by self-assembling a poly-seleno-amino acid amphiphilic block copolymer with a structure as shown in the following formula and then modifying the self-assembled poly-seleno-amino acid amphiphilic block copolymer with lactoferrin, where n is smaller than or equal to 454 and greater than or equal to 22, x is smaller than or equal to 50 and greater than or equal to 2, y is smaller than or equal to 50 and greater than or equal to 2, and n, x and y are all integers. The drug carrier disclosed by the invention can be used for loading drug molecules to prepare a sustained-release and controlled-release targeted drug delivery system, and can be used for directionally delivering the drug molecules to a diseased region so as to reduce the drug delivery frequency, improve the treatment effect and the like. When having the advantages of common amino acid drug carriers, the drug carrier not only has various biological functions of selenium, but also has specificity of the targeted lactoferrin receptor, is a novel targeted multifunctional drug carrier, and is suitable for research, development and clinical application of drug carriers of various drugs for various diseases related to lactoferrin receptor abnormality.

The present invention relates to the fields of biotechnology and medicine, specifically two anti-HER2 antibody-chaetocin conjugates Trastuzumab-vc-Chaetocin (T-L-C) and Pertuzumab-vc-Chaetocin (P-L-C) and their Synthetic preparation methods and applications. The antibody conjugate is obtained by conjugating a drug molecule with a linker and a monoclonal antibody, and its structure is shown below. The invention also provides a preparation method of the drug molecule with linking group. The antibody conjugate prepared by the present invention has a targeting effect on HER2-positive cells, which can not only enhance the killing of HER2-positive tumor cells, but also reduce the toxic and side effects caused by (+)-Chaetocin administered alone. Cell-induced cancers have enormous potential therapeutic applications.

The invention discloses a small molecule peptide and its preparation method and application as a nano drug carrier. The structural formula of the peptide is Ac-LVVLKKK(pY)-NH 2 , can self-assemble into spherical nanoparticles in Hepes solution. The spherical nanoparticles formed by the peptide can be used as a drug-carrying carrier to pack insoluble antitumor drugs, reduce their toxic and side effects, and improve their bioavailability. The small molecular polypeptide designed in the present invention has enzyme responsiveness and the ability of self-assembly morphology transformation, can respond to the alkaline phosphatase overexpressed in the tumor microenvironment, and carry insoluble anti-tumor drugs to tumor cells. Under the induction, it transforms from spherical nanoparticles into nanofibers, releases anti-tumor drugs and prolongs the residence time of drugs in cells. The invention discloses the construction, preparation method and application of the peptide, and provides a new idea for the development of the nano drug loading system of chemotherapy drugs.

The invention provides the application of peanut citrate transporter gene AhFRDL1 in improving plant resistance to aluminum toxicity stress. The application is to transfer the peanut citrate transporter gene AhFRDL1 or a recombinant expression vector containing the peanut citrate transporter gene AhFRDL1 into plant cells, screen transgenic plants, and make the transgenic plants express the peanut citrate transporter, thereby Improve plant resistance to aluminum toxicity stress. The present invention proves for the first time that the peanut citrate transporter gene AhFRDL1 is a gene that regulates the secretion of citric acid to the plant, and participates in the biological process of plants resisting aluminum toxicity stress. The peanut citrate transporter gene AhFRDL1 is used to improve the resistance of plants Under the stress of aluminum toxicity, it has long-term practical significance to further cultivate the main crops resistant to aluminum toxicity on acidic soil.