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Double layer composite collagen base guide tissue regeneration film and its preparing method

A technology that guides tissue regeneration and double-layer compounding. It is applied in medical science, surgery, etc. It can solve the problems of increasing the chance and impact of trauma, and the practicability of products with unstable preparation processes. It achieves excellent biocompatibility and promotes regeneration and repair. , to achieve the effect of regenerative healing

Inactive Publication Date: 2007-06-06
INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

From the late 1970s to the early 1980s, Nyman, Gottlow, Becker and others successively studied non-degradable materials such as polyacetal, polytetrafluoroethylene (PTFE), silicone membrane, etc. Good biocompatibility, but because it cannot be absorbed by the tissue, a second operation is required to remove the material, which increases the chance of trauma
In these research works, due to the limitation of the main material (such as the selection of type II collagen to make it more suitable for cartilage tissue) or the instability of the preparation process (such as the preparation of multilayer film materials by mechanical pressing, there may be split Separation of layer structure) affects the practicability of these developed products

Method used

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  • Double layer composite collagen base guide tissue regeneration film and its preparing method
  • Double layer composite collagen base guide tissue regeneration film and its preparing method

Examples

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Effect test

Embodiment 1

[0027] Adopt the method described in Chinese patent ZL94118836.1 (CN 1086145C) to extract and prepare the collagen swelling solution, the swelling solution is diluted to 0.6% with 0.3% malonic acid, and 100 grams of the swelling solution diluted with collagen is poured into a diameter of 100mm and a height of 2.5cm glass dish. The petri dish was first placed in a refrigerator at -35°C for 2 hours. After taking it out, the frozen petri dish was quickly placed on the shelf of a vacuum freeze dryer and freeze-dried for 50 hours under a vacuum of 10-100u. Take out the lyophilized collagen material from the lyophilizer and place it between two polytetrafluoroethylene plates, and flatten it with a smooth object such as a roller to form a relatively dense layer.

[0028] Dissolve 1 gram of medical-grade sodium hyaluronate in 50ml of water, oxidize with 50ml of 20mM sodium periodate solution, 5ml of 0.2M sodium perchlorate, 18 hours at 4°C, and slowly add 0.04M hydrogen sulfite dropwi...

Embodiment 2

[0033] The dense collagen layer and the treatment with sodium hyaluronate are the same as in Example 1.

[0034] The preparation process of the loose layer is as follows: Weigh an appropriate amount of collagen swelling solution and treated sodium hyaluronate, dilute and dissolve respectively, the concentration and ratio are the same as in Example 1, add the sodium hyaluronate solution into the collagen swelling solution, and use 0.1M Adjust the pH value to 7-9 with Tris base or 0.1M sodium bicarbonate solution, and shake it in a constant temperature air bath at 20°C for 24 hours at a low speed (<60 rpm) to make the oxidized sodium hyaluronate molecules and collagen A crosslinking reaction occurs. After the reaction, carefully discard the excess liquid, and prefreeze the remaining solid suspension according to the conditions in Example 1 until a certain viscous ice-water structure is formed to tightly cover the prepared dense layer, and continue to prefreeze until it is comple...

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Abstract

Collagen and hyaluronic acid or its sodium salt as main material are prepared into double layer composite material with compact layer and loose layer. The mass composition of the compact layer includes malonic acid swelling solution in 0.6-0.8 wt%; and the mass composition of the loose layer includes malonic acid swelling solution in 0.2-0.4 wt% and hyaluronic acid or its sodium salt in 5-20 wt% of dry collagen. The present invention has loose structure in one side and compact structure in the other side, and thus excellent biocompatibility capable of resisting the growth of fibroblast and other cell to the damaged part while promoting the regeneration and repairing of tissue in the damaged part. The double layer composite material has corresponding biodegrading function and may be degraded and absorbed without needing secondary operation to take out the implanted material. The present invention is suitable for repairing tissue damage.

Description

technical field [0001] The invention belongs to the field of biomedical materials, in particular to a double-layer composite collagen-based guided tissue regeneration material and a preparation method thereof. The invention is applicable to tissue defects caused by trauma, tumor surgery, etc., and is used for guided regeneration and repair of defective tissues, so as to realize regenerative healing of defective tissues. As a functional biomaterial, the material prepared by the invention can also be used as a tissue engineering scaffold for the research and development of tissue engineering artificial organs. technical background [0002] Medical guided tissue regeneration materials are an important part of medical biomaterials and a new type of biomaterials developed in recent years. The concept of guided tissue regeneration (guided tissue regeneration, GTR) was first proposed by Nyman et al. in the 1980s. It refers to relying on mechanical barriers to selectively guide cel...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/24A61L31/04A61L33/12
Inventor 张其清李学敏刘玲蓉亢凯
Owner INST OF BIOMEDICAL ENG CHINESE ACAD OF MEDICAL SCI
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