The application provides an asymmetric anti-adhesion
composite film and a preparation method and application thereof. The
composite film comprises a bioactive layer and an
electrospun fiber layer, and the
electrospun fiber layer comprises a first
fiber layer and a second
fiber layer. The first
fiber layer comprises fibers with a first
diameter. The second
fiber layer comprises fibers with the first
diameter and fibers with a second
diameter, and the first diameter is larger than the second diameter. The
composite film has strong mechanical and degradation resistance properties, and is suitable for anti-adhesion applications of various moving organoids or tissues, so that the composite film still has
structural integrity and
functional integrity for a long time in these environments. One side of the composite film has
adhesive fibers, and has stronger adhesion in a wet state, so that the composite film cannot be easily displaced when applied to moving organoids or tissues.