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Biobrick including endothelial cells and application thereof

A technology of endothelial cells and bio-bricks, applied in the field of biology, can solve the problems of size limitation, limited application of bioprinting technology, low survival rate of three-dimensional construct body cells, etc.

Active Publication Date: 2016-10-26
SICHUAN REVOTEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

This greatly limits the application of bioprinting technology
In addition, some known bioprinting techniques need to use a large number of cells when constructing micro-tissue blocks, which further severely limits the application of bioprinting techniques
[0007] Therefore, none of the currently known 3D bioprinting technologies can realize the construction of complex tissues or organs with three-dimensional structures in a manner that controls the number of cells and precisely arranges cells, and the cell survival rate of the prepared three-dimensional constructs is low, and the size severely restricted

Method used

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  • Biobrick including endothelial cells and application thereof
  • Biobrick including endothelial cells and application thereof
  • Biobrick including endothelial cells and application thereof

Examples

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preparation example Construction

[0276] The methods of the present invention for making constructs are compatible with other bioprinting methods known in the art. For example, the method of the present invention can be used in bioprinters developed by Cyfuse, Organovo and EnvisionTEC. Three main types of bioprinters have been developed, namely, inkjet bioprinters, extrusion bioprinters and laser-assisted bioprinters (see MurphSV and Atala A. (2014) Nature Biotechnology, 32(8): 773- 785). Alternatively, the methods of the present invention for making constructs can be used in bioprinting methods using automated or non-automated mechanical processes (rather than printers), or in bioprinting using manual placement or manual deposition methods (e.g. using pipettes) method. In certain preferred embodiments, bioprinting is performed by inkjet. In certain preferred embodiments, bioprinting is performed by extrusion. In certain preferred embodiments, bioprinting is performed by manual placement or manual depositi...

specific Embodiment approach

[0322] The invention will now be described with reference to the following examples, which are intended to illustrate the invention, but not to limit it.

[0323] Reagents, kits or instruments whose sources are not specified in the examples are all conventional products commercially available on the market. Those skilled in the art understand that the examples describe the present invention by way of example and are not intended to limit the scope of the claimed invention.

Embodiment 1

[0324] Embodiment 1. The preparation of biological brick

[0325] This example provides an exemplary method of making a biobrick. The preparation method of the bio-brick should be carried out under sterile conditions. In addition, if the bio-brick is intended to be applied to the human body, the biosafety level of its preparation method should reach the GMP workshop level.

[0326] The instrument used in this method is a granulator (BUCHI, Encapsulator, B-395Pro), and the diameters of the concentric nozzles it is equipped with are as follows: the inner layer nozzle, 200 μm; the outer layer nozzle, 300 μm.

[0327] The materials used in this method are as follows:

[0328] (1) Materials used to prepare the nuclear layer

[0329] Type I collagen: 4mg / ml, neutralized with sterile 1M NaOH;

[0330] Sodium alginate: Dissolve and dilute with deionized water;

[0331] Vascular endothelial growth factor VEGF;

[0332] Type I collagen was mixed with 2% (w / v) (ie, 2 g / 100 ml, the ...

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Abstract

The invention relates to the technical fields of biology, regenerative medicine, biological printing (e.g., 3D biological printing), tissue engineering, etc. Particularly, the invention relates to a biobrick applicable to biological printing (e.g., 3D biological printing) and tissue engineering, a composition (e.g., biological ink) including the biobrick, a preparation method and application of the biobrick and a constructed body (e.g., a three-dimensional constructed body) including the biobrick or constructed by using the biobrick / biological ink. The biobrick comprises cells, a core wrapping cells (e.g., endothelial cells), and a shell encapsulating the core.

Description

technical field [0001] The invention relates to the technical fields of biology, regenerative medicine, bioprinting (such as 3D bioprinting), tissue engineering and the like. In particular, the present invention relates to a bio-brick comprising endothelial cells that can be used for bioprinting (such as 3D bioprinting) and tissue engineering, a composition (such as bio-ink) comprising the bio-brick, a method for preparing the bio-brick As well as its use, as well as a microvessel-containing construct (such as a three-dimensional construct) comprising said biobrick or constructed using said biobrick / bioink. Background technique [0002] In human tissues, cells are arranged and distributed according to certain rules and orders. Tissues with different functions have different cell arrangements. For example, epithelial cells that make up epithelial tissue are closely arranged in a single layer to ensure their protective function; muscle cells are arranged in cords to ensure t...

Claims

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Application Information

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IPC IPC(8): A61L27/58A61L27/54A61L27/50A61L27/38C12Q1/02C12N5/07
CPCA61K9/5036A61K9/5052A61K35/12A61K35/28A61K35/44A61K35/545A61K49/0004A61L27/20A61L27/26A61L27/38A61L27/3804A61L27/3808A61L27/3834A61L27/50A61L27/507A61L27/54A61L27/58A61L2300/236A61L2300/252A61L2300/412A61L2300/414A61L2430/22B33Y70/00C12N5/06C12N5/0663C12N5/069C12N5/0697C12N2500/25C12N2500/30C12N2500/32C12N2501/15C12N2501/165C12N2501/998C12N2513/00C12N2533/54C12N2533/74C12Q1/02G01N33/5008G01N2500/10C08L5/04
Inventor 康裕建左潇
Owner SICHUAN REVOTEK CO LTD
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