41results about How to "Regulation of proliferation" patented technology

The invention discloses an application of an RSPO3 gene in sow ovarian granulosa cells. Two kinds of follicles with diameters of 3-5 mm and 7-10 mm respectively are used as experimental materials, andthe relationship between H3K4me3 and the expression level of genes related to ovarian follicle development is studied by a sequencing technology. The enrichment degree of H3K4me3 in a promoter regionof the RSPO3 gene is found to be significantly different in the follicles with different size by ChIP-Seq; the expression quantity of the RSPO3 gene in the follicles with different size is found to be significantly different by qRT-PCR; promotion of the degree of the H3K4me3 is found to promote transcription of the RSPO3 gene by promoting or inhibiting the degree of H3K4me3 in the sow ovarian granulosa cells, and inhibition of the degree of H3K4me3 is found to inhibit transcription of the RSPO3 gene; the RSPO3 is found to promote the proliferation of the sow ovarian granulosa cells and inhibit the apoptosis of the cells through overexpression or interference of RSPO3.

The invention discloses phenylalanine-derived chiral supramolecular hydrogel and application thereof, wherein the hydrogel forms a three-dimensional network through physical fiber winding, and the fiber aperture of the hydrogel is about 10 microns. The nanofiber in the LPG gel is in a left-handed spiral shape, and the nanofiber in the DPG gel is in a right-handed spiral shape, and the left and right spiral nanofibers have the same fiber diameter (50 + / - 10 nm) and screw pitch (480 + / - 40 nm). The phenylalanine-derived chiral supramolecular hydrogel is applied to the fields of ophthalmology andneuroscience, and is also applied to stem cells (such as retinal stem cells, neural stem cells and the like) derived from ectoderm.

The invention discloses a method for constructing plasmids of recombinant human SPINK5 genes and application of the plasmids. The method and the application have the advantages that the pathogenesis of melanoma with a high fatality rate is deeply explored, further research and development on novel therapeutic targets for inhibiting malignant behavior such as melanoma cell proliferation and invasion are carried out, and accordingly sufficient laboratory bases can be provided to clinical treatment for the melanoma; interference segments of coding genes SPINK5 of a Lympho-Epithelial Kazal-type related inhibitor (LETKI) are constructed and are applied to the aspects of melanoma cell proliferation and invasion, and accordingly novel experimental evidence can be provided to fundamental researchon the melanoma and research and development on clinical therapeutic targets. The full name of the SPINK5 is Serine Peptidase Inhibitor Kazal Type 5.

The invention discloses an adipose-derived stem cell-ECM modified SIS composite engineered bone and a preparation method thereof. The microstructure of the composite engineered bone comprises an SIS collagen fiber skeleton, human adipose-derived stem cells and an extracellular matrix layer, the extracellular matrix layer is composed of MC3T3-E1 cell guided and secreted matrix protein and polysaccharide, the extracellular matrix layer covers the surface of the SIS collagen fiber skeleton, and human adipose-derived stem cells adhere to the extracellular matrix layer. The composite engineered bone has high mechanical strength and a microstructure highly similar to natural bone, can provide good tissue support at the initial stage of implantation in vivo, can provide excellent bone-formation microenvironment for implantation of stem cells, after implantation, human adipose-derived stem cells can undergo multiplication and osteogenic differentiation rapidly, thus promoting rapid repair of large bone injury. In addition, the composite engineered bone has pure natural and endogenous characteristics, can avoid uncontrollable factors brought about by introduction of exogenous substances / genes and the like, has very good safety, and has wide clinical application prospects in bone regeneration field.

The invention discloses an application of an Interferon Regulating Factor 1 (IRF1) in regulating and controlling the immunomodulatory effect of human placenta, amnion and umbilical cord derived mesenchymal stem cells. The immunomodulatory effect is the effect of inhibiting T cell proliferation. The biological characteristics of human amniotic membrane, placenta and umbilical cord derived mesenchymal stem cells are researched by utilizing a single cell sequencing technology, and the IRF1 is found to be highly expressed in the placenta, amniotic membrane and umbilical cord derived mesenchymal stem cells in an inflammatory microenvironment. Transcription factor analysis and in-vitro experimental research further prove that the IRF1 can regulate and control the expression of MSC immunosuppressive molecules IDO1, CD274 and STAT1 and chemotactic factors CXCL9 and CXCL10, and in-vitro T cell proliferation experiments find that the IRF1 can regulate and control the T cell proliferation inhibition effect of the umbilical cord MSC, so that an important theoretical basis and a therapeutic target are provided for treatment of autoimmune diseases.

The invention discloses a traditional Chinese medicine for treating primary liver cancer. The traditional Chinese medicine comprises the following raw materials proportioning by weight: 20 to 30 parts of oldenlandia diffusa, 20 to 30 parts of lobelia, 20 to 30 parts of agrimonia pilosa, 20 to 30 parts of herba scutellariae barbatae, 10 to 20 parts of morel, 2 to 4 parts of aweto, 8 to 15 parts of flos buddlejae and 10 to 20 parts of glossy privet fruit, which are added with water and decocted into decoction. For a patient with liver ascites, 20 to 30 parts of herba rostellulariae and 10 to 15 parts of areca are added. The traditional Chinese medicine has the advantages of protecting the liver, promoting liver cell regeneration, expanding the blood vessel of the liver, reinforcing the blood circulation in the liver and increasing the blood flow of the liver, thereby improving liver nutrition and oxygen supply and reinforcing the immunological function. The sources of all the medicinal materials are extensive, the medicinal materials can be mostly collected in southern regions, various Chinese herbal medicines are adopted to carry out comprehensive treatment with obvious effect and convenient taking, each prescription composition conforms to the prescription of medicine politics, the synthetic action of each medicine is utilized to treat the liver cancer, so that the traditional Chinese medicine has no toxic or side effect on a human body.

The invention provides a method for adjusting and controlling ovarian granulosa cells based on circ-Atp5BP. The method comprises the following steps: (1) carrying out inoculation and subculture on nest granulosa cells; and (2) when the cell density is cultured to be 35 to 45 percent, transfecting the circ-Atp5BP gene or the pik3 gene into the nest granular cells. The application of circ-Atp5BP and pik3 in porcine ovarian granular cell proliferation adjustment and the targeting adjustment relation between circ-Atp5BP and pi3k are proved for the first time. The proliferation of the ovarian granular cells can be remarkably adjusted and controlled.

The invention relates to the technical fields of biology, regenerative medicine, biological printing (such as 3D biological printing), tissue engineering and the like. Particularly, the invention relates to a biological brick which can be used for biological printing (such as 3D biological printing) and tissue engineering, a composition (such as biological ink) containing the biological brick, a preparation method and application of the biological brick, and a structure (such as a three-dimensional structure) containing the biological brick or constructed by using the biological brick / biological ink, wherein the biological brick comprises cells, a core layer wrapping the cells (such as endothelial cells), and a shell layer packaging the core layer.

The present invention relates to a transgenic pig that expresses sTNFR1-Fc, wherein a gene encoding sTNFR1-Fc, which is a fusion protein of the extracellular domain of human soluble tumor necrosis factor receptor (sTNFR1) and an immunoglobulin Fc region, is introduced; a method for preparing the same; an organ isolated from the transgenic pig; a somatic donor cell line inserted with sTNFR1-Fc gene; a method for preparing a blood sample comprising sTNFR1-Fc; and a method for preparing human sTNFR1-Fc from the blood sample of the transgenic pig. As the transgenic pig can suppress immune response and inflammatory response by secreting an inhibitory substance that suppresses the activity of TNF-α in blood, it can be effectively used for xenograft. Furthermore, since the transgenic pig has a blood type O, it can be transplanted for suppressing inflammatory response, regardless of a blood type of recipient.

The invention discloses a fat stem cell-ECM modified SIS composite engineered bone and a preparation method thereof. The microstructure of the composite engineered bone includes a SIS collagen fiber skeleton, human fat stem cells and an extracellular matrix layer, and the extracellular matrix layer is composed of MC3T3‑E1 Cell-guided and secreted matrix proteins and polysaccharides, the extracellular matrix layer covers the surface of the SIS collagen fiber skeleton, and human adipose stem cells adhere to the extracellular matrix layer; the composite engineered bone has high mechanical strength and is highly similar to natural The microstructure of bone can provide good tissue support at the initial stage of implantation in the body, and can provide an excellent osteogenic microenvironment for stem cell implantation. After implantation, human adipose stem cells can rapidly proliferate and differentiate into osteoblasts, and promote the healing of large bone injuries. Rapid repair; in addition, the composite engineered bone has pure natural endogenous characteristics, which can avoid uncontrollable factors caused by the introduction of foreign substances / genes, etc., has good safety, and has broad clinical application prospects in the field of bone regeneration .

The present invention provides a method for controlling the proliferation and differentiation of cord blood-derived hematopoietic stem cells with excellent safety when proliferating them by culturing. The hematopoietic stem cells are inoculated into a medium containing a sonicated liquid component of cord blood. The proliferation and differentiation of the cord blood hematopoietic stem cells can be inhibited in the presence of the sonicated liquid component of cord blood. On the contrary, the proliferation of cord blood hematopoietic stem cells can be accelerated by inoculating the hematopoietic stem cells into a medium containing a non-sonicated liquid component of cord blood. Thus, according to the present invention, by using serum derived from cord blood, it is possible to regulate the inhibition of the proliferation and differentiation of cord blood hematopoietic stem cells and the acceleration of the proliferation of the same as desired.

Application of circular RNA hsa_circ_0004872 in the diagnosis of gastric cancer. The present invention belongs to the field of molecular biology and provides a circular RNA, the nucleotide sequence of the gene of the circular RNA hsa_circ_0004872 is shown in SEQ ID NO.1, and the circular RNA hsa_circ_0004872 is from the MAPK1 gene of chromosome 22 The 2nd, 3rd, and 4th exons of MAPK1 pre-mRNA are reversely cleaved, and the AG base at the 3' end of the 4th exon of MAPK1 pre-mRNA is connected with the CT base at the 5' end of the 2nd exon, forming a covalently closed loop shape RNA. The circular RNA hsa_circ_0004872 can be used as a gastric cancer diagnostic marker in the preparation of gastric cancer diagnostic reagents.

The invention relates to a care agent for improving the infant skin barrier efficacy and a preparation process thereof. A plant composition is added in the care agent; meanwhile, the care agent consists of a phase A, a phase B and a phase C. The ingredients and the proportion of the phase A, the phase B and the phase C are optimized; and meanwhile, a preparation method of the care agent is provided. Used raw materials pass toxicological and safety performance tests and are strictly selected; the use safety of a skin care product is ensured; on the basis of the characteristics of the infant skin, pure natural skin care grease is used; and a product is particularly suitable for the tender skin of infants. Through confirmation by experiments, the care agent is safe and non-irritant on the skin; the effects of improving the skin barrier, restoring the damaged skin in an auxiliary way and improving the skin state such as skin elasticity and moisture content increase are achieved; the raw materials of a formula can be easily obtained; the preparation method is simple and convenient; green effects and safety are realized; the care agent can be easily accepted by people groups; and good application prospects and market prospects are realized.

Novel use of circular RNA hsa_circ_0004872 for gastric cancer therapy. The present invention belongs to the field of molecular biology and provides a circular RNA, the nucleotide sequence of the gene of the circular RNA hsa_circ_0004872 is shown in SEQ ID NO.1, and the circular RNA hsa_circ_0004872 is from the MAPK1 gene of chromosome 22 The 2nd, 3rd, and 4th exons of MAPK1 pre-mRNA are reversely cut, and the AG base at the 3' end of the 4th exon of MAPK1 pre-mRNA is connected with the CT base at the 5' end of the 2nd exon, forming a covalently closed loop shape RNA. The circular RNA hsa_circ_0004872 can be used in the preparation of chemotherapy drugs for gastric cancer.

The invention discloses an anti-tumor combined drug and its application in the preparation of anti-cancer drugs. The combined drug includes a first preparation and a second preparation, and the anti-tumor combined drug is the first preparation in parts by weight. 10-40 parts of the preparation, 0.1-10 parts of the second preparation. The invention also discloses the use of chlorogenic acid in the preparation of inhibitors of toxic and side effects of anticancer drugs, and the anticancer drugs are BCR‑ABL small molecule inhibitors. The technical scheme of the present invention can improve the targeting of BCR-ABL small molecule inhibitors in chronic myeloid leukemia, reduce its toxic and side effects, and reverse the drug resistance of BCR-ABL small molecule inhibitors.

The invention discloses the application of RSPO3 gene in sow ovary granulosa cells. The invention uses two kinds of follicles with a diameter of 3-5 mm and 7-10 mm as experimental materials, and uses sequencing technology to study the relationship between H3K4me3 and expression levels of genes related to ovarian follicle development. Through ChIP-Seq, it was found that the enrichment degree of H3K4me3 in the promoter region of RSPO3 gene was significantly different in follicles of different sizes; through qRT-PCR, it was found that the expression of RSPO3 gene in different sizes of follicles was significantly different; by promoting or inhibiting maternal The level of H3K4me3 in porcine ovarian granulosa cells was found to promote the level of H3K4me3 can promote the transcription of RSPO3 gene, and inhibit the level of H3K4me3 can inhibit the transcription of RSPO3 gene; by overexpressing or interfering with RSPO3, it was found that RSPO3 can promote the proliferation of sow ovary granulosa cells and inhibit cell apoptosis .

The invention relates to cream with the effect of improving an infant skin barrier and a preparing process thereof. An effective plant composition is added into the cream, and meanwhile the cream is composed of a phase A, a phase B and a phase C, the component and ratio of the phase A, the phase B and the phase C are optimized, and the preparing method of the cream is provided. Raw materials in use pass toxicity and safety tests and are strictly selected to ensure safe use of the skin care product, based on the characteristics of infant skin, pure natural skin care grease is adopted, the cream is particularly suitable for the tender infant skin, it is proved through tests that the cream is safe and non-irritant to the skin and has the effects of improving the skin barrier, assisting in repairing damaged skin and improving the skin state such as improving skin elasticity and increasing water content, the component raw materials are easy to obtain, the preparing method is simple, green and safe, and the cream is easy to accept by people groups and has excellent application prospects and market prospects.