153 results about "Group A - blood" patented technology

The invention provides an SNP (Single Nucleotide Polymorphism) site of an ABO blood group variant, and a B subtype variant blood group identification kit and method, and relates to the technical field of biology. The SNP (Single Nucleotide Polymorphism) sites of the ABO blood group variant comprise the 842th c.842Cgt from an initiation codon of an ABO * B.01 allele coding region of an ABO gene; mutation of T. A substance for detecting the SNP site of the ABO blood group variant can be applied to preparation of a B subtype variant blood group identification method and product, so that the substance can be effectively applied to rapid detection of patients and blood donors, the B subtype variant blood group of an individual can be defined, and potential possible blood transfusion reaction can be prevented in time.

The invention discloses for the first time pluripotent cells, including induced pluripotent stem cells, embryonic stem cells, hypo-immune pluripotent cells, cells that have been derived therefrom, and cells that have been biologically differentiated therefrom into particular tissue lineages that are ABO blood type O Rhesus Factor negative or otherwise evade rejection resulting from blood type antigen mismatch. The invention further provides universally acceptable “off-the-shelf” pluripotent cells and derivatives thereof for generating or regenerating specific tissues and organs.

The invention discloses an anti-idiotype antibody for detecting and neutralizing an anti-D antibody and application of the anti-idiotype antibody, the anti-idiotype antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises at least one of an HCDR1 sequence, an HCDR2 sequence and an HCDR3 sequence, and the amino acid sequence of the HCDR1 has the amino acid sequence shown as SEQ ID No.9; the amino acid sequence of the HCDR2 has an amino acid sequence as shown in SEQ ID No. 10; the amino acid sequence of the HCDR3 has the amino acid sequence as shown in SEQ ID No. 11. The anti-idiotype antibody can be specifically combined with an anti-D antibody, and can be applied to clinical blood type detection or prevention or treatment of anti-D antibody related diseases.

The invention discloses an anti-idiotype antibody for neutralizing an anti-D antibody, the anti-idiotype antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises at least one of an HCDR1 sequence, an HCDR2 sequence and an HCDR3 sequence, and the amino acid sequence of the HCDR1 has an amino acid sequence as shown in SEQ ID No.25; the amino acid sequence of the HCDR2 has an amino acid sequence as shown in SEQ ID No. 26; the amino acid sequence of the HCDR3 has an amino acid sequence as shown in SEQ ID No. 27. The anti-idiotype antibody can be specifically combined with an anti-D antibody, and can be applied to clinical blood type detection or prevention or treatment of anti-D antibody related diseases.

The invention discloses a neutralizing anti-idiotype antibody of an anti-D antibody, the neutralizing anti-idiotype antibody comprises a heavy chain variable region and a light chain variable region, the heavy chain variable region comprises at least one of HCDR1, HCDR2 and HCDR3 sequences, the amino acid sequence of the HCDR1 is as shown in SEQ ID No.1, the amino acid sequence of the light chain variable region is as shown in SEQ ID No.2, the amino acid sequence of the light chain variable region is as shown in SEQ ID No.3, and the amino acid sequence of the light chain variable region is as shown in SEQ ID No.4. The amino acid sequence of the HCDR2 is as shown in SEQ ID No. 2; the amino acid sequence of the HCDR3 is as shown in SEQ ID No. 3. The neutralizing anti-idiotype antibody has the effect of neutralizing anti-D-mediated erythrocyte agglutination, can be used for detecting a mixed sample containing various anti-D blood group accidental antibodies, and can also be used for risk assessment and clinical diagnosis and treatment by using Rh-HDFN.

The invention relates to the technical field of blood inventory management, in particular to a radio frequency tag reuse method and system for blood inventory management. Comprising the following steps that blood is collected, radio frequency tags corresponding to carriers are distributed, information of the radio frequency tags is read, the blood is put in storage, different kinds of blood are recorded and stored in the corresponding carriers, and rechecking is conducted through the number of the empty carriers; the blood storage information is acquired, the blood information is classified, the ex-warehouse quantity of blood of different blood types every day is acquired, and the blood demand quantity of different blood types is predicted, the demand quantity is predicted based on the ex-warehouse quantity, blood sampling and stock adjustment strategies can be planned in advance, blood shortage or excess is avoided, scientific classification and fine management of blood are achieved, and the blood quality is improved. The clinical blood use requirement is better met, the real-time stocktaking and alarming functions can remind workers to supplement insufficient blood types in time, and the timeliness and stability of blood supply are ensured.

The invention relates to the field of blood type detection, and particularly discloses a full-automatic blood type analyzer and a control system thereof, the quality of blood type test paper is evaluated by monitoring the damage degree, the smudginess degree and the wrinkle degree of the blood type test paper, early warning is carried out, replacement of the blood type test paper is reminded, and the situation that the blood type test paper with unqualified quality is used in blood type detection is avoided; the quality of the blood sample is evaluated by monitoring the time for collecting the blood sample, and the blood type test paper is replaced to collect the blood sample again, so that the use of unqualified blood samples in blood type detection is avoided; the temperature, the humidity and the light intensity of a blood type testing environment are collected, the influence coefficient of the testing environment on a measurement result is analyzed, whether the testing environment is poor or not is judged, and early warning and prompting are carried out; the detection accuracy and reliability of the blood type analyzer are improved from multiple dimensions, so that the blood type analyzer plays a better role in the scenes of medical first aid preliminary blood type judgment, blood type screening on a blood donation site, family self-blood type detection and the like.

The invention discloses an intelligent intensive blood sampling management system based on a GPU, and belongs to the technical field of blood sampling management. The intelligent intensive blood sampling management system based on the GPU comprises a data acquisition unit and a data analysis unit. The problem that efficient management of the intensive blood sampling process is difficult to achieve in the prior art is solved, bar code and two-dimensional code information in the sample image can be rapidly and accurately recognized through the deep learning algorithm based on GPU acceleration, the sample information extraction speed and accuracy are improved, and the efficiency of blood sampling is improved. The trained deep learning model is used for analyzing the sample images, blood types and detection items can be automatically identified, errors caused by manual judgment are effectively avoided, samples are accurately classified according to the analysis result, the sample processing efficiency is improved, an automatic sorting device is accurately controlled according to the classification result, and the sorting efficiency is improved. And the sample test tubes are conveyed to designated positions, so that manual intervention is reduced, and meanwhile, the sorting accuracy and efficiency are improved.

This invention discloses a method for preparing A, B, and Lewis blood group antigens, wherein the A, B, and Lewis blood group antigens are specifically blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigen. A one-pot photosensitive method was used to achieve blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b The method for obtaining large quantities of antigens, as described in this invention, features mild reaction conditions, shortened purification steps, simplicity, high efficiency, environmental friendliness, low cost, and high yield and purity of the target substance. This invention provides a practical and rapid chemical synthesis route for blood type A antigens. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigens provide tools for the development of norovirus vaccines and related antibodies, and therefore have great application prospects.

The invention relates to a blood type specificity IgG antibody typing detection reagent card, a kit and a preparation method thereof. The blood type specificity IgG antibody typing detection reagent card comprises a plurality of accommodating holes and information identifiers, magnetic beads coated with anti-erythrocyte antibodies, acridinium ester-labeled anti-human IgG antibodies of different subtypes, and a working solution are respectively arranged in the accommodating holes. The preparation method comprises the following steps: preparing magnetic beads coated with anti-erythrocyte antibodies and different subtypes of anti-human IgG antibodies marked by acridinium ester; respectively mixing the magnetic beads coated with the anti-erythrocyte antibodies and the acridinium ester-labeled anti-human IgG antibodies of different subtypes with a protection solution; and the information identification is arranged on the outer side of the containing hole. The blood type specificity IgG antibody typing detection kit comprises the reagent card, a pre-excitation solution and an excitation solution. The reagent card is high in specificity, easy and convenient to operate and suitable for large-scale clinical detection, and the detection efficiency and accuracy are improved.

The present application discloses a blood type card puncturing and sample addition system, including a workbench, and a circulating conveying assembly, a puncturing device and a sample addition device installed on the workbench, wherein the circulating conveying assembly includes an annular conveying bar, a power device and a plurality of blood type card carrier boxes, the power device acts on the conveying bar and can drive the conveying bar to rotate along a circular route, the blood type card carrier box is connected to the conveying bar and can be driven to move by the conveying bar; the puncturing device and the sample addition device are sequentially arranged at different positions of the circular route, and correspond to the top of the blood type card carrier box. The present invention arranges a circulating conveying assembly, and a plurality of blood type card carrier boxes are circulated, and puncturing, sample addition and robot picking can be operated independently and mutually unaffected, and the overall structure is compact, the volume is small, and the work efficiency is greatly improved.

1. The name of the product of this design: blood type analyzer. 2. Purpose of the product of this design: medical testing instrument used to analyze and detect blood type. 3. The design key point of this appearance design product lies in the shape. 4. The picture or photo that best illustrates the key points of the design: main view.

The present application relates to the technical field of biomedical equipment, and particularly provides a blood type detector and a shaking and mixing device of the blood type detector, aiming to solve the problem that the mixing effect of blood samples and detection reagents of the existing blood type detector is poor, thereby affecting the detection result. To this end, the shaking and mixing device comprises a shaking and mixing mechanism and a cup loading component mounted on the shaking and mixing mechanism, the cup loading component is used for loading a reaction cup, and the shaking and mixing mechanism can vibrate with the cup loading component and the reaction cup in the cup loading component to mix the blood sample and the detection reagent in the reaction cup. By arranging the cup loading component on the shaking and mixing mechanism, the reaction cup can be directly fixed on the shaking and mixing mechanism, so that the vibration generated by the shaking and mixing mechanism can be better transmitted to the reaction cup, thereby improving the mixing effect of the blood sample and the detection reagent in the reaction cup, and further improving the accuracy of the detector.

The invention relates to the technical field of science popularization teaching aids, in particular to a blood type measuring science popularization teaching aid and a use method thereof, and the blood type measuring science popularization teaching aid comprises an operation table, a display screen, an A blood type typing resisting reagent area, a B blood type typing resisting reagent area and an extraction assembly; the extraction assembly comprises four blood simulation discs, four positioning bodies, a supporting driving mechanism, a rotating shaft, a rotating disc, four rebound mechanisms and four positioning mechanisms; the four blood simulation discs with the four positioning bodies are respectively inserted into the four slots and are respectively fixed by matching the positioning mechanisms with the positioning bodies; the support driving mechanism drives the rotating shaft to rotate and drives the turntable to rotate; after the rotating disc rotates for a plurality of circles, one blood simulation disc is randomly selected, the positioning mechanism of the blood simulation disc is loosened, the corresponding rebound mechanism pushes out a part of the blood simulation disc and the positioning body, and then the blood simulation disc and the positioning body are taken out together, so that the blood simulation disc can be randomly drawn, and the authenticity and challenge of an experiment are improved.

1. The name of this design product: Blood type test card. 2. Purpose of this design product: This design product is a test card used to detect the blood type of humans or animals. 3. The key point of the design of this product lies in its shape. 4. The picture or photo that best illustrates the design points: three-dimensional picture.

The blood type detection card comprises a main body, limiting clamping grooves are formed in the two sides of the front end of the upper surface of the main body, a plurality of detection blocks are fixedly connected to the middle of the upper surface of the main body, an inlet is formed in the center of the upper surface of each detection block, and the limiting clamping grooves are communicated with the limiting clamping grooves. The rear end of the upper surface of the main body is fixedly connected with a plurality of detection strips, and the outer wall of the rear end of the main body is fixedly connected with a flexible connecting strip. Compared with the prior art, the blood type detection card provided by the utility model is small in size and simple in structure, the blood type detection card is convenient to use due to the arranged specification card, so that a worker can carry the blood type detection card conveniently, and can carry out a test conveniently as required; therefore, the use convenience and the application range of the blood type detection card are improved.

The utility model relates to a simple and easy device for detecting human blood type, including flushing liquid containing container, detection subassembly and liquid suction piece, flushing liquid containing container has the containing cavity that contains flushing liquid, and containing cavity has top opening to allow detection subassembly inserts containing cavity, and containing cavity inboard wall is provided with first limit piece, the antibody for blood type detection is fixed in detection subassembly, the antibody can be combined with blood type antigen specificity, detection subassembly has first end and second end, liquid suction piece detachably fixed in second end of detection subassembly, detection subassembly outside wall is provided with second limit piece, wherein, when first end of detection subassembly inserts containing cavity, first limit piece and second limit piece cooperate and form longitudinal limit to fix detection subassembly in containing cavity, the utility model has the characteristics of simple operation, high accuracy, fast detection.

The invention discloses a risk assessment system for difficulty in cross matching of blood, and relates to the technical field of clinical medical examination and blood transfusion management. The system comprises a data input module, a risk calculation module, a risk layering module and a result output and suggestion module. The data input module obtains key information of a patient, wherein the key information comprises an erythrocyte irregular antibody screening result, multiple myeloma diagnosis, hematology department medical history, age of more than or equal to 60 years, pregnancy-related disease state and blood type identification difficulty; the risk calculation module adopts a preset scoring algorithm to endow each factor with a weight and calculates a total risk score; the risk layering module divides the patients into three levels of low risk, medium risk and high risk; and the result output module provides differentiated clinical management paths corresponding to the risk levels. According to the method, early warning of difficulty in cross matching of blood is achieved through quantitative evaluation, blood transfusion process efficiency can be optimized, clinical risks can be reduced, and the method has good prediction performance (AUC = 0.819) and a high negative prediction value (93.7%).

The invention provides a freeze-dried universal platelet rich plasma (PRP) preparation and a preparation process thereof, aiming at solving the problems that the existing PRP is limited in blood type matching, difficult in biological activity preservation, short in validity period and the like. According to the preparation, 18-19% of the type A, 7-8% of the type B and 73-74% of the type AB are mixed with PRP of different blood types and freeze-dried. Tests show that agglutination does not exist in cross blood matching, the titers of the anti-A antibody and the anti-B antibody are both lower than 1: 2, and blood group reaction is effectively avoided. Freeze-drying causes little damage to growth factors, still has biological activity within 24 months, and has a good cell proliferation test effect. Animal experiments show that wound healing can be remarkably promoted. In addition, the culture medium is good in stability in the environment of 4 DEG C, and the moisture, growth factors and the sterile state all meet the requirements. The preparation disclosed by the invention has the advantages of obvious universal advantages, good biological activity preservation and strong stability, and provides a better choice for clinical treatment.

The invention aims to provide a gene identification method for an A-type variant blood group in an ABO blood group system, namely, a screening method for the A-type variant blood group is established on the basis that an SNP site which can cause wrong typing of the ABO blood group is obtained through screening. The SNP sites are located at 188-190 sites of an A subtype gene with a nucleotide sequence of SEQ ID NO: 1, and are A deletion. The invention provides a new application of ABO blood type A variant gene detection, thereby providing a gene diagnosis, prenatal gene screening and genetic counseling approach for effectively avoiding blood type error typing and acute hemolytic transfusion reaction. The application effect shows that the SNP site of the gene and the detection primer provided by the invention can be effectively used for rapid detection of the ABO blood type A variant gene mutation site in peripheral blood of a clinical patient.

The invention belongs to the technical field of data storage, and particularly discloses a full-automatic blood type analyzer data storage safety management method, which comprises the following steps of: classifying blood type detection data generated by a full-automatic blood type analyzer to construct blocks, and calculating an original hash value of each block to form a data chain; complete relevance storage of the blood type detection data is realized, the integrity of each data block is ensured, and any data tampering can cause the change of the hash value, so that the data change can be found in time, and the detection efficiency is improved. In addition, when it is recognized that the data chain changes, local locking operation or all locking operation is intelligently selected by combining the system performance monitoring data at the change moment and the proportion condition of the change blocks, and the flexibility of the locking operation after data changes is displayed. According to the method, system performance reduction caused by forced execution of all locking is avoided, and data locking is ensured on the premise that the system stability is maximized.

The present invention discloses a neutralizing anti-idiotypic antibody of an anti-D antibody, comprising a heavy chain variable region and a light chain variable region, wherein the heavy chain variable region comprises at least one of HCDR1, HCDR2, and HCDR3 sequences, wherein: the amino acid sequence of the HCDR1 is shown in SEQ ID No. 1; the amino acid sequence of the HCDR2 is shown in SEQ ID No. 2; and the amino acid sequence of the HCDR3 is shown in SEQ ID No. 3. This neutralizing anti-idiotypic antibody has the ability to neutralize anti-D-mediated hemagglutination of red blood cells and can be used to detect mixed samples containing multiple blood type antibodies unexpectedly containing anti-D. It can also be used for risk assessment and clinical diagnosis and treatment of Rh-HDFN.

The invention discloses a blood type and microbiological detection data correlation analysis method based on a statistical model, and belongs to the technical field of medical treatment. Through data preprocessing and feature fusion, standardized processing and dimensionality reduction expression of blood type information and microbiological detection high-dimensional features are realized, and a joint feature matrix is constructed; an integrated Gaussian mixture network is adopted to carry out multi-level distribution modeling, and high-resolution clustering of microbial spectrums of different blood group populations is realized in combination with a category cascade discrimination mechanism. Further, a complex nonlinear relation between the blood type and the microorganism data is dynamically analyzed in a multi-scale space by utilizing a self-adaptive multi-scale correlation mining algorithm. Then, screening and analyzing key causal variable pairs of the blood type-microbiome through sparse regression and causal inference by adopting an interpretable correlation modeling and variable screening method; the accuracy and generalization ability of correlation analysis are improved, and accurate prediction and high-reliability risk judgment are realized.

The utility model discloses a blood scraping spoon for a blood type detection experiment, and belongs to the technical field of medical experiment equipment. The spoon comprises a rod body, a spoon body a and a spoon body b, the rod body is a long-strip-shaped cylinder, the spoon body a is fixed to one end of the rod body, the spoon body b is fixed to the other end of the rod body, and the rod body, the spoon body a and the spoon body b are of an integrated structure and are made of straw regeneration degradable materials; the spoon body a and the spoon body b are in small spoon shapes with thin-walled edges and are the same in structure, one ends, far away from the rod body, of the spoon body a and the spoon body b are linear, and openings of the spoon body a and the spoon body b face the same direction; the length of the rod body is 7 cm, the diameter of the rod body is 3-4 mm, and the inner diameter of the spoon body a and the inner diameter of the spoon body b are 4-5 mm. The rod body is divided into two identical parts, one part of the rod body and the spoon body a are light blue, and the other part of the rod body and the spoon body b are light yellow. According to the utility model, peripheral blood can be fully scraped, two antibodies can be effectively ensured not to be confused in the process of uniformly mixing the antibodies and the blood, and the correctness of a result is ensured.

1. The name of the design product: full-automatic blood type analyzer. 2. The use of the design product: using column agglutination method, together with the matching detection reagent, for ABO system blood type typing, Rh system blood type typing, irregular antibody screening, cross matching and direct anti-human globulin test on human blood samples in clinical application. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

To provide pluripotent cells including induced pluripotent stem cell, embryonic stem cells and hypo-immune pluripotent cells that are ABO blood type O Rhesus Factor negative and evade rejection resulting from blood type antigen mismatch.SOLUTION: Provided is a modified pluripotent cell or a cell derived therefrom where the cell is ABO blood type O Rhesus Factor negative (Rh-), and the cell has: (a) reduced or eliminated ABO blood type antigen selected from the group consisting of A1, A2 and B; and / or (b) reduced or eliminated expression of Rh protein antigen selected from the group consisting of Rh C antigen, Rh E antigen, Kell K antigen (KEL), Duffy (FY) Fya antigen, Duffy Fy3 antigen, Kidd(JK), Jkb antigen, MNS antigen U and MNS antigen S.SELECTED DRAWING: None

The invention provides an SNP (Single Nucleotide Polymorphism) marker of an Rh blood group system and application of the SNP marker in detecting RhD variation phenotypes. RhD variation blood groups of red blood cells can be confirmed by detecting an SNP site. The SNP marker is located at the 121st basic group of a nucleotide fragment of SEQ ID NO: 1, that is, the 922nd basic group from an initiation codon in an RHD gene coding region is mutated, and G is mutated into C. The SNP marker of an Rh blood group system is obtained through detection and screening, and whether an individual to be detected has an RhD variation phenotype or not is determined by detecting whether the SNP marker exists or not. The application effect shows that the SNP locus and the detection primer provided by the invention can be effectively used for rapid detection of the RHD gene of clinical patients and blood station blood donors.

1. Name of the Design Product: Blood Type Detection Plate (Capsule Type). 2. Use of the Design Product: For detecting blood types. 3. Design Key Points of the Design Product: The combination of shape and pattern. 4. Picture or Photograph that Best Illustrates the Design Key Points: Perspective view.

The present invention belongs to the field of biological detection technology, and provides a CD38 monoclonal antibody-MOFs composite biomaterial. The CD38 monoclonal antibody-MOFs composite biomaterial includes a core and a coating layer, wherein the core is a MOFs composite biomaterial, and the coating layer is a CD38 monoclonal antibody or an irregular blood type antibody. In the CD38 monoclonal antibody-MOFs composite biomaterial provided by the present invention, Zu-MOF as a host not only does not affect the antibody's ability to detect its corresponding antigen, but also can be stored for a long time, avoiding high temperature and biological damage. The use of the CD38 monoclonal antibody-MOFs composite biomaterial provided by the present invention helps to dissipate the agglutination caused by the interference of the CD38 monoclonal antibody and improve the accuracy of the blood matching results.

Provided are a primer and probe composition for cat blood type detection, a detection method, and use. The primer and probe composition for detecting the wild-types and the mutants of the above four sites is designed according to the single nucleotide polymorphism (SNP) mutation of genotyping of 179G>T, 268T>A, 364C>T and 1322delT four sites of a cat cytidine monophospho-N-acetylneuraminic acid (CMAH) gene; an amplification curve is plotted by utilizing a real-time fluorescence polymerase chain reaction (PCR) technology, and the recognition of bases of four SNP sites is achieved by analysis of the amplification curve, so as to determine cat blood types. The detection method of the present disclosure is carried out entirely in a closed space, thereby avoiding the problem of environmental aerosol pollution.