42 results about "Group A - blood" patented technology

This invention discloses a method for preparing A, B, and Lewis blood group antigens, wherein the A, B, and Lewis blood group antigens are specifically blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigen. A one-pot photosensitive method was used to achieve blood group A. tri Antigen, B tri Antigen, Le a Antigen and Le b The method for obtaining large quantities of antigens, as described in this invention, features mild reaction conditions, shortened purification steps, simplicity, high efficiency, environmental friendliness, low cost, and high yield and purity of the target substance. This invention provides a practical and rapid chemical synthesis route for blood type A antigens. tri Antigen, B tri Antigen, Le a Antigen and Le b Antigens provide tools for the development of norovirus vaccines and related antibodies, and therefore have great application prospects.

The present application relates to the technical field of biomedical equipment, and particularly provides a blood type detector and a shaking and mixing device of the blood type detector, aiming to solve the problem that the mixing effect of blood samples and detection reagents of the existing blood type detector is poor, thereby affecting the detection result. To this end, the shaking and mixing device comprises a shaking and mixing mechanism and a cup loading component mounted on the shaking and mixing mechanism, the cup loading component is used for loading a reaction cup, and the shaking and mixing mechanism can vibrate with the cup loading component and the reaction cup in the cup loading component to mix the blood sample and the detection reagent in the reaction cup. By arranging the cup loading component on the shaking and mixing mechanism, the reaction cup can be directly fixed on the shaking and mixing mechanism, so that the vibration generated by the shaking and mixing mechanism can be better transmitted to the reaction cup, thereby improving the mixing effect of the blood sample and the detection reagent in the reaction cup, and further improving the accuracy of the detector.

The invention relates to the technical field of science popularization teaching aids, in particular to a blood type measuring science popularization teaching aid and a use method thereof, and the blood type measuring science popularization teaching aid comprises an operation table, a display screen, an A blood type typing resisting reagent area, a B blood type typing resisting reagent area and an extraction assembly; the extraction assembly comprises four blood simulation discs, four positioning bodies, a supporting driving mechanism, a rotating shaft, a rotating disc, four rebound mechanisms and four positioning mechanisms; the four blood simulation discs with the four positioning bodies are respectively inserted into the four slots and are respectively fixed by matching the positioning mechanisms with the positioning bodies; the support driving mechanism drives the rotating shaft to rotate and drives the turntable to rotate; after the rotating disc rotates for a plurality of circles, one blood simulation disc is randomly selected, the positioning mechanism of the blood simulation disc is loosened, the corresponding rebound mechanism pushes out a part of the blood simulation disc and the positioning body, and then the blood simulation disc and the positioning body are taken out together, so that the blood simulation disc can be randomly drawn, and the authenticity and challenge of an experiment are improved.

The utility model relates to a simple and easy device for detecting human blood type, including flushing liquid containing container, detection subassembly and liquid suction piece, flushing liquid containing container has the containing cavity that contains flushing liquid, and containing cavity has top opening to allow detection subassembly inserts containing cavity, and containing cavity inboard wall is provided with first limit piece, the antibody for blood type detection is fixed in detection subassembly, the antibody can be combined with blood type antigen specificity, detection subassembly has first end and second end, liquid suction piece detachably fixed in second end of detection subassembly, detection subassembly outside wall is provided with second limit piece, wherein, when first end of detection subassembly inserts containing cavity, first limit piece and second limit piece cooperate and form longitudinal limit to fix detection subassembly in containing cavity, the utility model has the characteristics of simple operation, high accuracy, fast detection.

The invention discloses a risk assessment system for difficulty in cross matching of blood, and relates to the technical field of clinical medical examination and blood transfusion management. The system comprises a data input module, a risk calculation module, a risk layering module and a result output and suggestion module. The data input module obtains key information of a patient, wherein the key information comprises an erythrocyte irregular antibody screening result, multiple myeloma diagnosis, hematology department medical history, age of more than or equal to 60 years, pregnancy-related disease state and blood type identification difficulty; the risk calculation module adopts a preset scoring algorithm to endow each factor with a weight and calculates a total risk score; the risk layering module divides the patients into three levels of low risk, medium risk and high risk; and the result output module provides differentiated clinical management paths corresponding to the risk levels. According to the method, early warning of difficulty in cross matching of blood is achieved through quantitative evaluation, blood transfusion process efficiency can be optimized, clinical risks can be reduced, and the method has good prediction performance (AUC = 0.819) and a high negative prediction value (93.7%).

The invention provides a freeze-dried universal platelet rich plasma (PRP) preparation and a preparation process thereof, aiming at solving the problems that the existing PRP is limited in blood type matching, difficult in biological activity preservation, short in validity period and the like. According to the preparation, 18-19% of the type A, 7-8% of the type B and 73-74% of the type AB are mixed with PRP of different blood types and freeze-dried. Tests show that agglutination does not exist in cross blood matching, the titers of the anti-A antibody and the anti-B antibody are both lower than 1: 2, and blood group reaction is effectively avoided. Freeze-drying causes little damage to growth factors, still has biological activity within 24 months, and has a good cell proliferation test effect. Animal experiments show that wound healing can be remarkably promoted. In addition, the culture medium is good in stability in the environment of 4 DEG C, and the moisture, growth factors and the sterile state all meet the requirements. The preparation disclosed by the invention has the advantages of obvious universal advantages, good biological activity preservation and strong stability, and provides a better choice for clinical treatment.

The invention discloses a blood type and microbiological detection data correlation analysis method based on a statistical model, and belongs to the technical field of medical treatment. Through data preprocessing and feature fusion, standardized processing and dimensionality reduction expression of blood type information and microbiological detection high-dimensional features are realized, and a joint feature matrix is constructed; an integrated Gaussian mixture network is adopted to carry out multi-level distribution modeling, and high-resolution clustering of microbial spectrums of different blood group populations is realized in combination with a category cascade discrimination mechanism. Further, a complex nonlinear relation between the blood type and the microorganism data is dynamically analyzed in a multi-scale space by utilizing a self-adaptive multi-scale correlation mining algorithm. Then, screening and analyzing key causal variable pairs of the blood type-microbiome through sparse regression and causal inference by adopting an interpretable correlation modeling and variable screening method; the accuracy and generalization ability of correlation analysis are improved, and accurate prediction and high-reliability risk judgment are realized.

1. The name of the design product: full-automatic blood type analyzer. 2. The use of the design product: using column agglutination method, together with the matching detection reagent, for ABO system blood type typing, Rh system blood type typing, irregular antibody screening, cross matching and direct anti-human globulin test on human blood samples in clinical application. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The application discloses a risk assessment system for cross matching difficulty, and relates to the technical field of clinical medical examination and blood transfusion management. The system comprises a data input module, a risk calculation module, a risk stratification module and a result output and suggestion module. The data input module obtains key information of a patient, including irregular red blood cell antibody screening results, multiple myeloma diagnosis, hematology department medical history, age of 60 years or older, pregnancy-related disease state and blood type identification difficulty; the risk calculation module adopts a preset scoring algorithm to assign weights to each factor and calculate a total risk score; the risk stratification module divides the patient into three grades of low risk, medium risk and high risk; and the result output module provides a differentiated clinical management path corresponding to the risk grade. The application realizes early warning of cross matching difficulty through quantitative assessment, can optimize the efficiency of the blood transfusion process and reduce clinical risk, has good prediction performance (AUC=0.819) and a high negative predictive value (93.7%).

A disposable blood typing device is provided for determining ABO and RhD blood types using binary logic-based interpretation. The device includes a sample application area, test zones containing dried anti-A, anti-B, and optionally anti-D reagents, and a result output mechanism configured to display a binary outcome for each antigen. Binary values are assigned based on visible agglutination reactions, which are processed through a logical mapping structure—such as a printed chart, mechanical indicator, or electronic logic circuit—to determine the corresponding blood type. The device may incorporate visual indicators, control regions, waste containment, and optional digital components for electronic display or wireless data transmission. Designed for single use, the system facilitates self-administered or point-of-care testing without requiring clinical expertise, and may be integrated with mobile applications or health records systems. The invention enhances test accuracy, usability, and data interoperability while minimizing contamination risk and interpretation error.

The present application relates to the technical field of biomedical equipment, and particularly provides a blood type detector, aiming to solve the problem of low automation degree of the existing blood type detector, resulting in poor user experience. To this end, the blood type detector comprises a shell, a feeding device and a sample filling device installed in the shell, the feeding device comprises a feeding mechanism and a fixing mechanism for carrying a blood collection tube, the feeding mechanism can move with the fixing mechanism and the blood collection tube fixed on the fixing mechanism to make the blood collection tube communicate with the sample filling device, the sample filling device can fill the blood sample in the blood collection tube into a reaction cup, and the feeding mechanism can also cooperate with the sample filling device to unload the blood collection tube on the fixing mechanism from the fixing mechanism. Through such a setting, the detector of the present application can automatically complete the feeding and unloading of the blood collection tube, thereby improving the automation degree of the detector.

The invention relates to a blood type auxiliary study and judgment method, which comprises the following steps: performing mixing, centrifugation and reaction treatment on a weak agglutination classified blood sample in blood type detection operation, performing precipitation on the sample, and analyzing the light transmittance to obtain the light transmittance variance of the sample and the reactivity and reaction acceleration of the reaction sample. And performing threshold comparison and judgment by combining a light transmission change rate upper limit calibration threshold, a light transmission change acceleration upper limit calibration threshold and a local turbidity gradient variance upper limit calibration threshold under dynamic adjustment of a temperature adjustment factor reflected by the real-time temperature, so as to determine the optimal precipitation time of the sample, effectively control the precipitation time to be too large, and simultaneously ensure the precipitation accuracy of the sample. It is guaranteed that the sample can fully react under the optimal precipitation duration, then the blood detection process is accurately controlled, blood type study and judgment are assisted, and the method has wide application prospects in practical application.

This invention provides a simple, rapid, low-cost, high-throughput, and accurate integrated solution that eliminates the need for nucleic acid extraction and purification. It enables targeted capture, amplification, and first-generation sequencing analysis of all key regions of the ABO gene directly from various clinical samples, including whole blood, saliva, oral swabs, and dried blood spots. The operation is simple and low-cost, thus achieving accurate ABO blood typing, subtype analysis, and the discovery of new variants. The method described in this invention can be applied to the identification of difficult blood types, and can discover new ABO alleles, blood group gene chimeras, and rare blood types such as subtype-related intron mutations.

The invention discloses a blood group reagent quality control kit and application thereof, the blood group reagent quality control kit comprises a plurality of independently subpackaged composite quality control products, and each quality control product simultaneously comprises quality control red blood cells with known antigen spectra and quality control plasma with known antibody spectra; the quality control red blood cells are human red blood cells with known ABO blood type, RhD blood type and Rh haplotype; the quality control plasma contains blood group antibodies with known specificity and immune globulin types, and the blood group antibodies comprise IgM antibodies and / or IgG antibodies. The invention also discloses application of the blood group reagent quality control kit in indoor quality control of blood group serology detection and an indoor quality control method of blood group serology detection. The kit provided by the invention can realize integrated and efficient detection, and synchronously complete indoor quality control of a plurality of core items such as blood type identification, antibody screening, cross matching of blood and the like.

The invention relates to the technical field of blood collection devices, and particularly discloses a blood collection device for blood type detection, which comprises a box body, an automatic blood collection device, a power supply module and a tablet computer. The automatic blood collection device is arranged on the lower portion of the box body, an opening is formed in the middle of the box body, the bottom face of the box body is inclined upwards, the power supply module is arranged on the upper portion of the box body, and the tablet computer is assembled on the top face of the box body. And the working part of the circulating jacking mechanism is vertical to the bottom surface of the opening and faces the bottom surface. The bottom surface of the opening is provided with a collecting hole for the capillary needle tube to penetrate through, the two supplementing units are arranged corresponding to the circulating jacking mechanism and respectively push the capillary needle tube and the liquid collecting cup to the working part, and the circulating jacking mechanism is matched with the two supplementing units, can jack the liquid collecting cup and is assembled with the liquid collecting cup in a clamping manner, and drives the assembly body to move towards the collecting hole, so that the collecting end of the capillary needle tube penetrates through the collecting hole. The purposes of reducing the manual labor intensity of blood type detection and improving the blood sample collection efficiency are achieved.

The invention relates to a primer group for MNS blood type genotyping detection, a kit and application, and belongs to the field of biomedicine clinical molecular detection. The primer group comprises one pair of amplification primers designed according to a GYPA gene specific sequence and five sequencing primers, and one pair of amplification primers designed according to a GYPB gene specific sequence and five sequencing primers. The specific amplification primer can be used for accurately and specifically amplifying the GYPA and the GYPB respectively, and the specific sequencing primer can be used for carrying out high-resolution sequencing reaction on the GYPA and GYPB gene subtypes. The invention has the following technical effects: the specificity of the GYPA and GYPB gene specific amplification primers designed by adopting a mismatch and double specific base combination method is high, and the GYPA and GYPB gene subtypes can be accurately distinguished by matching with the specific sequencing primers provided by the invention. The kit provided by the invention can be used for accurately judging the MNS blood type genetic typing of an experimental sample. The method is quick and simple to operate and low in cost, and has wide application prospect and clinical reference value.

The application discloses a multiplex PCR detection system, a kit and application for RH gene typing detection. The application can efficiently and accurately identify common Rh genotypes in Asian population through a single PCR amplification reaction. The application not only effectively makes up for the limitations of traditional serological methods in detecting complex blood types such as D variant, but also has the advantages of high standardization, low cost, simple operation and low clinical promotion threshold.

The invention discloses a Taq enzyme for detecting an Asian DEL blood type, a PCR reaction solution and application of the Taq enzyme and the PCR reaction solution. The amino acid sequence of the Taq enzyme for detecting the Asian DEL blood type is as shown in SEQ ID NO: 2. The conventional detection point mutation PCR needs to detect the genome DNA concentration and dilute nucleic acid according to the detection result so as to prevent the generation of a false positive result, and the reason is that the PCR is not sensitive enough to the difference generated by the mutation of a single basic group, and the difference between a mutant type and a wild type cannot be effectively expanded. The Taq enzyme constructed and expressed by the invention can effectively distinguish the difference of single bases on a genome. The generation of false positive reaction results can still be avoided when the concentration of the genome reaches 30mu g / mL.

This invention discloses a primer set, kit, and application for prenatal screening of RhD, belonging to the field of gene detection technology. Based on genetic data of the Chinese population, this invention proposes four specific primer pairs. Through specific amplification, it can effectively distinguish four genes: RhD complete deletion gene, RhD-CE(2-9)-D hybrid gene, RhD1227A, and RhD wild-type gene. Compared with existing technologies, by incorporating RhD exon9 amplification, it can detect the RhD1227A gene, which accounts for approximately 16.3% to 32.6% of RhD-negative blood types in the Chinese population, reducing the probability of false positives due to paternal RhD1227A gene carriage. This provides a more accurate, low-cost, rapid, and convenient option for prenatal screening of RhD in the Chinese population.

The application provides an agglutination strength interpretation method and device and a blood type analyzer, and relates to the medical field. The agglutination strength interpretation method photographs a blood type card located on the blood type analyzer to obtain a first image, wherein the blood type card comprises a plurality of microcolumn tubes containing blood gel, and the blood gel comprises serum, red blood cells and gel liquid; a template image is acquired; a target region of the first image is identified according to the acquired template image, and the target region is used to indicate the region of the blood gel on the first image; and the agglutination strength of the blood gel in the target region of the first image is interpreted. After the blood type analyzer photographs the blood type card to obtain the first image, the template image can be acquired, and the position of the blood gel on the template image is accurate. In this way, the accuracy of interpreting the agglutination strength of the blood gel in the target region of the first image is also high.

This invention provides a SNP site, identification kit, identification method, and application for the Jk(a+w) blood type, which triggers immune hemolytic transfusion reactions. The SNP site is the c.613C>T mutation at position 613, starting from the start codon of the SLC14A1 gene coding region. In the proband, the c.613C>T mutation was detected in one allele, while the other allele carried other known inactivating mutations. The allele carrying the c.613C>T mutation resulted in reduced expression of the Kidd protein in the proband's erythrocytes; the other allele, carrying other inactivating mutations, did not express the Kidd protein. The combined effect of these two mutated alleles resulted in the Jk(a+w) phenotype. This provides a genetic variation basis for the establishment of rare blood type banks and the assurance of transfusion compatibility.

1. The name of the design product: blood type analyzer. 2. The use of the design product: medical testing instrument, for analyzing and detecting the blood type of blood. 3. The design points of the design product: in shape. 4. The picture or photo that best indicates the design points: perspective view.

The invention relates to a hemophilia A perioperative period blood coagulation factor drug delivery model and application thereof, and relates to the technical field of biological information. The hemophilia A perioperative period blood coagulation factor administration model is constructed by adopting a nonlinear mixed effect modeling method based on a two-chamber model structure, and covariants of the hemophilia A perioperative period blood coagulation factor administration model comprise blood type, body weight, age, perioperative period anti-fibrinolytic drug use condition and perioperative period NSAID analgesic drug use condition NSAID. The model can be used for predicting an FVIII concentration-time curve of a clinical individual patient, and individual fine optimization of the administration dosage and the administration time is realized.

The present application relates to the technical field of biomedical equipment, and particularly provides a blood type detector, aiming to solve the problem of low automation degree of the existing blood type detector, resulting in poor user experience. For this purpose, the blood type detector comprises a shell, a consumable storage component, a shaking and mixing device and a loading and unloading device installed in the shell. The consumable storage component can store a plurality of reaction cups. The shaking and mixing device can carry the reaction cup and mix the blood sample added into the reaction cup with the detection reagent. The loading and unloading device can load the reaction cup in the consumable storage component onto the shaking and mixing device and unload the reaction cup from the shaking and mixing device. Through such a setting, the detector of the present application can automatically realize the loading and unloading of the reaction cup, improve the automation degree of the detector, and provide better user experience.

The utility model discloses a blood type detection placing rack, which comprises a middle placing rack, a side placing rack and a connecting structure, the middle placing rack comprises a bottom plate, two groups of middle test tube racks respectively and fixedly arranged on the upper surface of the bottom plate, and middle detection card placing frames respectively and fixedly arranged on the opposite surfaces of the two groups of middle test tube racks; the side placing frames are arranged on the two sides of the middle placing frame respectively. According to the blood type detection placing rack, the middle placing rack, the side placing racks and the connecting structures are arranged, test tubes and blood type detection cards are placed on the middle placing rack and the side placing racks, and when the test tubes and the blood type detection cards need to be taken and reagents need to be dropped, the two side placing racks can be pulled towards the two sides, so that the test tubes and the blood type detection cards are placed on the connecting structures; therefore, the side detection card placing frame between the two adjacent middle detection card placing frames can be pulled out, so that the distance between the side detection cards and the middle detection cards is increased, and convenience is brought to the use of medical staff.

The application discloses a Taq enzyme for detecting Asian DEL blood type, a PCR reaction solution and application thereof. The Taq enzyme for detecting Asian DEL blood type has an amino acid sequence shown in SEQ ID NO: 2. The conventional PCR for detecting point mutation needs to detect the concentration of genomic DNA, and dilute nucleic acid according to the detection result, so as to prevent the generation of false positive results. The reason is that the difference generated by PCR to single base mutation is not sensitive enough, and the difference between the mutant and the wild type cannot be effectively distinguished. The expressed Taq enzyme can effectively distinguish the single base difference on the genome. The concentration of the genome reaches 30 mu g / mL, and the generation of false positive reaction results can still be avoided.

The utility model discloses a reagent classification storage frame special for blood transfusion laboratory, reagent classification storage frame special for blood transfusion laboratory includes setting on the first storage area of base, second storage area, third storage area and fourth storage area, set at least one first installation groove for the installation irregular antibody screening cell reagent on the first storage area, set at least one second installation groove for the installation blood type reverse type reagent on the second storage area, set at least one third installation groove for the installation blood type positive type and Rh (D) blood type type reagent on the third storage area, and the fourth storage area is provided with the fourth installation groove for the installation agglutination amine cross matching blood reagent, the utility model discloses four storage areas and sets up the installation groove of corresponding specification reasonably, realizes the orderly classification and fixed placement of reagent, effectively avoids the risk of reagent bottle falling and taking confusion, simultaneously, through the corresponding design of name mark and installation groove, further improves the accuracy and efficiency of reagent taking.

The utility model discloses a position correcting mechanism and a blood type analyzer, and relates to the technical field of blood type analyzers, the position correcting mechanism comprises a position correcting assembly and a driving assembly, and the position correcting assembly is arranged below a carrier and is provided with a movably arranged position correcting end; the driving assembly is in transmission connection with the position correcting assembly, so that the position correcting assembly is provided with a first position spaced from the carrier and a second position abutting against the carrier; when the position correcting assembly is located at the second position, the end face of the position correcting end horizontally extends and is used for being connected with the bottom end of the carrier in an abutting mode so that the carrier can be corrected to be in the horizontal state; according to the technical scheme, the position correcting mechanism capable of correcting the carrier can be provided.

The utility model provides a blood type percolation detection card which can be used for identifying the blood type of a person or an animal and comprises an upper shell, a lower shell and a test strip, the upper shell is provided with a sample adding hole, the lower shell is provided with a limiting groove, the test strip is fixed in the limiting groove, a protrusion is arranged in the limiting groove, and the protrusion can jack up the test strip to prevent the test strip from making contact with the lower shell and is used for accelerating the capillary action, so that the result obtaining speed is increased; the clinging degree of the test strip and the sample adding hole can be adjusted; the periphery of the sample adding hole is provided with an elliptical recess for storing a dropped blood sample or flushing fluid; a bulge is also arranged on the back surface of the elliptical recess and is used for reinforcing and fixing the test strip. The device has the advantages of simplicity in manufacturing, small size, light weight, low price, short detection time and accurate detection result, and is suitable for large-scale detection.

The invention relates to a blood typing kit designed to make blood typing more flexible, whether in or out of the laboratory. The kit comprises coverslips containing ready-made antibodies of anti-A, anti-B, and anti-D sera, a lancet in addition to the coverslips, and compressed alcohol cotton for sterilization.