The invention relates to the technical field of medical experimental apparatus. Provided are cross-matching of blood, blood types A, B, O, and Rh D, and irregular antibody screening sample-adding machine. The screening sample-adding machine is provided with a distribution pipe with both ends sealed and characterized in that at least one injector is arranged side by side on the distribution pipe; needle parts of the injectors penetrating the distribution pipe are located at the lower end of the distribution pipe; radial through holes are arranged on the injectors inside the distribution pipe; a sample-adding pipe is arranged on the distribution pipe next to each injector; the sample-adding pipes are communicated with the injectors through the distribution pipe. The sample-adding machine solves the problems that sample-adding machines special for cross-matching of blood, blood types A, B, O, and Rh D, and irregular antibody screening experiments do not exist and it is complex, inefficient, and error-prone to operate with ordinary straw or dropper. cross-matching of blood, blood types A, B, O, and Rh D, and irregular antibody screening sample-adding machine are provided.