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Novel use of circular RNA hsa_circ_0004872 for gastric cancer therapy

A ring-shaped, gastric cancer technology, applied in the field of molecular biology, can solve problems such as the poor five-year survival rate of gastric cancer patients, the median survival period of patients with gastric cancer metastasis is less than one year, and the limited survival period of gastric cancer patients, and achieve the goal of promoting proliferation Effect

Inactive Publication Date: 2021-04-02
SHANDONG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, these treatments have limited significance in improving the survival period of gastric cancer patients, making the five-year survival rate of gastric cancer patients poor, and the median survival period of patients with gastric cancer metastasis is less than one year

Method used

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  • Novel use of circular RNA hsa_circ_0004872 for gastric cancer therapy
  • Novel use of circular RNA hsa_circ_0004872 for gastric cancer therapy
  • Novel use of circular RNA hsa_circ_0004872 for gastric cancer therapy

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 Expression of hsa_circ_0004872 in gastric cancer

[0030] 1. hsa_circ_0004872 is a circular RNA formed by reverse splicing of exons 2, 3, and 4 of MAPK1

[0031] We screened the differentially expressed circular RNAs in gastric cancer and corresponding paracancerous tissues by using the next-generation circular RNA sequencing technology, and selected hsa_circ_0004872 and hsa_circ_0001566, which were significantly differentially expressed, as our candidate molecules for research. Through qRT-PCR detection of fresh gastric cancer tissues and corresponding paracancerous tissues, it was found that the expression of hsa_circ_0004872 was significantly down-regulated in gastric cancer tissues (85.7% p<0.0001), although hsa_circ_0001566 was also down-regulated in gastric cancer tissues (69.56%, p<0.0001). 0.0001), but not as obvious as hsa_circ_0004872, so we choose hsa_circ_0004872 (see Table 1 for the sequence, sequence number SEQ ID NO.1) as our research object.

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Embodiment 2

[0042] Example 2 The effect of hsa_circ_0004872 on the proliferation, invasion and metastasis of gastric cancer cells can significantly inhibit the proliferation of gastric cancer cells

[0043] 1. Effect of overexpression of hsa_circ_0004872 on proliferation, invasion and metastasis of gastric cancer cells

[0044] To study the effect of hsa_circ_0004872 on the biological effects of gastric cancer cells, we constructed the hsa_circ_0004872 overexpression vector pLCDH-circ-4872. It and the control vector were transfected into BGC-823 and SGC-7901 respectively. The results of qRT-PCR showed that after transfection of the overexpression plasmid, the expression level of hsa_circ_0004872 in gastric cancer cells was significantly increased ( figure 2 A).

[0045] After transfecting the hsa_circ_0004872 overexpression plasmid, the cell proliferation efficiency was detected by EdU assay and CCK8 assay, and it was found that overexpression of hsa_circ_0004872 significantly inhibited...

Embodiment 3

[0051] Example 3 The molecular mechanism of hsa_circ_0004872 inhibiting the proliferation, invasion and metastasis of gastric cancer cells

[0052] 1. Subcellular localization of hsa_circ_0004872 in gastric cancer cells

[0053] Since the functions of circRNAs are usually related to their different localizations in cells, current studies have shown that circRNAs formed by exons usually exist in the cytoplasm. To detect the localization of hsa_circ_0004872 in gastric cancer cells, we performed fluorescence in situ hybridization (FISH) experiments. We designed the hsa_circ_0004872 probe with Cy3 red fluorescent label (see Table 3 for the sequence). After the gastric cancer cells grew on the cell slides, the cells were fixed and permeabilized, and the Cy3 red fluorescently labeled probe was hybridized with the cell slides overnight, stained with DAPI, sealed, and photographed under a laser confocal microscope. The experimental results show that: hsa_circ_0004872 mainly exists i...

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Abstract

Novel use of circular RNA hsa_circ_0004872 for gastric cancer therapy. The present invention belongs to the field of molecular biology and provides a circular RNA, the nucleotide sequence of the gene of the circular RNA hsa_circ_0004872 is shown in SEQ ID NO.1, and the circular RNA hsa_circ_0004872 is from the MAPK1 gene of chromosome 22 The 2nd, 3rd, and 4th exons of MAPK1 pre-mRNA are reversely cut, and the AG base at the 3' end of the 4th exon of MAPK1 pre-mRNA is connected with the CT base at the 5' end of the 2nd exon, forming a covalently closed loop shape RNA. The circular RNA hsa_circ_0004872 can be used in the preparation of chemotherapy drugs for gastric cancer.

Description

technical field [0001] The invention belongs to the field of molecular biology, and in particular relates to a novel use of circular RNA hsa_circ_0004872 for gastric cancer treatment. Background technique [0002] Gastric cancer is a common malignant tumor of the digestive system. It is one of the fifth most common cancers in the world. Its mortality rate ranks third in the global cancer mortality rate. In 2018 alone, there were more than 1 million new gastric cancer patients, and it is estimated that they died of gastric cancer More than 783,000 patients. The traditional treatment for gastric cancer is surgical treatment, with radiotherapy or chemotherapy as adjuvant treatment. However, these treatments have limited significance in improving the survival period of gastric cancer patients, making the five-year survival rate of gastric cancer patients poor, and the median survival period of patients with gastric cancer metastasis is less than one year. Therefore, it is extr...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12Q1/6886C12N15/113
CPCC12Q1/6886C12Q2600/158C12Q2600/178
Inventor 刘志方马存英王效莹臧翊辰
Owner SHANDONG UNIV
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