Biological brick containing endothelial cells and application of biological brick

A technology of endothelial cells and bio-bricks, which is applied in the field of biology and can solve the problems of low survival rate of three-dimensional constructs, restrictions on the application of bioprinting technology, and size limitations.

Active Publication Date: 2018-05-29
SICHUAN REVOTEK CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This greatly limits the application of bioprinting technology
In addition, some known bioprinting techniques need to use a large number of cells when constructing micro-tissue blocks, which further severely limits the application of bioprinting techniques
[0008] Therefore, none of the currently known 3D bioprinting technologies can realize the construction of complex tissues or organs with three-dimensional structures in a manner that controls the number of cells and precisely arranges cells, and the cell survival rate of the prepared three-dimensional constructs is low, and the size severely restricted

Method used

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  • Biological brick containing endothelial cells and application of biological brick
  • Biological brick containing endothelial cells and application of biological brick
  • Biological brick containing endothelial cells and application of biological brick

Examples

Experimental program
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preparation example Construction

[0277] The methods of the present invention for making constructs are compatible with other bioprinting methods known in the art. For example, the method of the present invention can be used in bioprinters developed by Cyfuse, Organovo and EnvisionTEC. Three main types of bioprinters have been developed, namely, inkjet bioprinters, extrusion bioprinters and laser-assisted bioprinters (see Murph SV and Atala A. (2014) Nature Biotechnology, 32(8): 773 -785). Alternatively, the methods of the present invention for making constructs can be used in bioprinting methods using automated or non-automated mechanical processes (rather than printers), or in bioprinting using manual placement or manual deposition methods (e.g. using pipettes) method. In certain preferred embodiments, bioprinting is performed by inkjet. In certain preferred embodiments, bioprinting is performed by extrusion. In certain preferred embodiments, bioprinting is performed by manual placement or manual deposit...

specific Embodiment approach

[0323] The invention will now be described with reference to the following examples, which are intended to illustrate the invention, but not to limit it.

[0324] Reagents, kits or instruments whose sources are not specified in the examples are all conventional products commercially available on the market. Those skilled in the art understand that the examples describe the present invention by way of example and are not intended to limit the scope of the claimed invention.

Embodiment 1

[0325] Embodiment 1. The preparation of biological brick

[0326] This example provides an exemplary method of making a biobrick. The preparation method of the bio-brick should be carried out under sterile conditions. In addition, if the bio-brick is intended to be applied to the human body, the biosafety level of its preparation method should reach the GMP workshop level.

[0327] The instrument used in this method is a granulator (BUCHI, Encapsulator, B-395Pro), and the diameters of the concentric nozzles it is equipped with are as follows: the inner layer nozzle, 200 μm; the outer layer nozzle, 300 μm.

[0328] The materials used in this method are as follows:

[0329] (1) Materials used to prepare the nuclear layer

[0330] Type I collagen: 4mg / ml, neutralized with sterile 1M NaOH;

[0331] Sodium alginate: Dissolve and dilute with deionized water;

[0332] Vascular endothelial growth factor VEGF;

[0333] Type I collagen was mixed with 2% (w / v) (ie, 2 g / 100 ml, the ...

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Abstract

The invention relates to the technical fields of biology, regenerative medicine, biological printing (such as 3D biological printing), tissue engineering and the like. Particularly, the invention relates to a biological brick which can be used for biological printing (such as 3D biological printing) and tissue engineering, a composition (such as biological ink) containing the biological brick, a preparation method and application of the biological brick, and a structure (such as a three-dimensional structure) containing the biological brick or constructed by using the biological brick / biological ink, wherein the biological brick comprises cells, a core layer wrapping the cells (such as endothelial cells), and a shell layer packaging the core layer.

Description

[0001] This application is a divisional application of the application date of April 7, 2016, the title of the invention is "a bio-brick containing endothelial cells and its use", and the application number is 201610213282.2. technical field [0002] The invention relates to the technical fields of biology, regenerative medicine, bioprinting (such as 3D bioprinting), tissue engineering and the like. In particular, the present invention relates to a bio-brick comprising endothelial cells that can be used for bioprinting (such as 3D bioprinting) and tissue engineering, a composition (such as bio-ink) comprising the bio-brick, a method for preparing the bio-brick As well as its use, as well as a microvessel-containing construct (such as a three-dimensional construct) comprising said biobrick or constructed using said biobrick / bioink. Background technique [0003] In human tissues, cells are arranged and distributed according to certain rules and orders. Tissues with different ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61L27/58A61L27/54A61L27/50A61L27/38C12Q1/02C12N5/07
CPCA61K9/5036A61K9/5052A61K35/12A61K35/28A61K35/44A61K35/545A61K49/0004A61L27/20A61L27/26A61L27/38A61L27/3804A61L27/3808A61L27/3834A61L27/50A61L27/507A61L27/54A61L27/58A61L2300/236A61L2300/252A61L2300/412A61L2300/414A61L2430/22B33Y70/00C12N5/06C12N5/0663C12N5/069C12N5/0697C12N2500/25C12N2500/30C12N2500/32C12N2501/15C12N2501/165C12N2501/998C12N2513/00C12N2533/54C12N2533/74C12Q1/02G01N33/5008G01N2500/10C08L5/04
Inventor 康裕建左潇
Owner SICHUAN REVOTEK CO LTD
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