Adipose-derived stem cells-ecm modified sis composite engineered bone and its preparation method

An adipose stem cell and human adipose stem cell technology, which is used in medical science, prosthesis, tissue regeneration, etc., can solve problems such as uncontrollable factors, and achieve the effects of good safety, high mechanical strength, and excellent biocompatibility.
CN106963986BActive Publication Date: 2020-05-26河南源创生命干细胞库科技有限公司 +1
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
河南源创生命干细胞库科技有限公司
Publication Date
2020-05-26

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Abstract

The invention discloses a fat stem cell-ECM modified SIS composite engineered bone and a preparation method thereof. The microstructure of the composite engineered bone includes a SIS collagen fiber skeleton, human fat stem cells and an extracellular matrix layer, and the extracellular matrix layer is composed of MC3T3‑E1 Cell-guided and secreted matrix proteins and polysaccharides, the extracellular matrix layer covers the surface of the SIS collagen fiber skeleton, and human adipose stem cells adhere to the extracellular matrix layer; the composite engineered bone has high mechanical strength and is highly similar to natural The microstructure of bone can provide good tissue support at the initial stage of implantation in the body, and can provide an excellent osteogenic microenvironment for stem cell implantation. After implantation, human adipose stem cells can rapidly proliferate and differentiate into osteoblasts, and promote the healing of large bone injuries. Rapid repair; in addition, the composite engineered bone has pure natural endogenous characteristics, which can avoid uncontrollable factors caused by the introduction of foreign substances / genes, etc., has good safety, and has broad clinical application prospects in the field of bone regeneration .
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Description

technical field

[0001] The invention relates to the technical field of tissue engineering and stem cell therapy in biomedicine, in particular to an adipose stem cell-ECM modified SIS composite engineering bone applicable to bone regeneration and a preparation method thereof. Background technique

[0002] About 3.5 million people in my country have bone defects due to different reasons every year, and about 1.5 million bone surgery transplants are performed. The demand for replacement bones is very large, especially for replacement bones for large bone injuries. Bone repair in vivo is a synergistic process between cells and scaffold materials. The three-dimensional microstructure of scaffold materials can provide host cells with a good microenvironment for adhesion, proliferation and migration. With the rapid development of tissue engineering technology, the use of tissue engineering principles to combine cells and scaffold materials in vitro and implant them into the body fo...

Claims

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