Adipose-derived stem cells-ecm modified sis composite engineered bone and its preparation method

An adipose stem cell and human adipose stem cell technology, which is used in medical science, prosthesis, tissue regeneration, etc., can solve problems such as uncontrollable factors, and achieve the effects of good safety, high mechanical strength, and excellent biocompatibility.

Active Publication Date: 2020-05-26
河南源创生命干细胞库科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, stem cell therapy has shown good tissue regeneration ability, but there are still great difficulties in improving the efficiency of cell therapy, mainly the following two problems: ① introduction of exogenous substances or the use of genetic engineering techniques to induce the differentiation of stem cells, There are many uncontrollable factors; ②When stem cells are injected into the body, how to quickly adhere and fuse into the recipient tissue to exert their functions is still under debate. Therefore, improving the efficiency of cell therapy is an issue that remains to be studied

Method used

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  • Adipose-derived stem cells-ecm modified sis composite engineered bone and its preparation method
  • Adipose-derived stem cells-ecm modified sis composite engineered bone and its preparation method
  • Adipose-derived stem cells-ecm modified sis composite engineered bone and its preparation method

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Embodiment 1

[0027] The preparation method of the fat stem cell-ECM modified SIS composite engineered bone of embodiment 1 comprises the following aseptic operation steps:

[0028] 1) Osteoblast culture and passage: Prepare α-MEM medium containing 10% fetal bovine serum as the osteoblast culture medium; place MC3T3-E1Subclone 14 cells isolated from the MC3T3-E1 cell line of the anterior cranial bone of the mouse into Cultivate in a cell culture incubator with 5% carbon dioxide concentration and 37°C, discard the old culture medium every 48 hours and add an equal amount of new culture medium, and carry out cell passage when the confluence of MC3T3-E1Subclone 14 cells is greater than 90% , the process of subculture of the cells is as follows: discard the old culture medium, add PBS buffer solution without calcium and magnesium ions, and wash twice; then add 1 mL of 0.25 wt% trypsin containing EDTA, and incubate at 37°C for 1 min; Collect the cells and add them to a centrifuge tube, centrifug...

Embodiment 2

[0032] The preparation method of the fat stem cell-ECM modified SIS composite engineered bone of embodiment 2 comprises the following aseptic operation steps:

[0033] 1) Osteoblast culture and passage: Prepare α-MEM medium containing 10% fetal bovine serum as the osteoblast culture medium; place MC3T3-E1Subclone 14 cells isolated from the MC3T3-E1 cell line of the anterior cranial bone of the mouse into Cultivate in a cell culture incubator with 5% carbon dioxide concentration and 37°C, discard the old culture medium every 48 hours and add an equal amount of new culture medium, and carry out cell passage when the confluence of MC3T3-E1Subclone 14 cells is greater than 90% , the process of subculture of the cells is as follows: discard the old culture medium, add PBS buffer without calcium and magnesium ions, wash 3 times; then add 1.5mL of 0.25wt% trypsin containing EDTA, and incubate at 37°C for 1min ;Collect the cells and add them to the centrifuge tube, and centrifuge at 1...

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Abstract

The invention discloses a fat stem cell-ECM modified SIS composite engineered bone and a preparation method thereof. The microstructure of the composite engineered bone includes a SIS collagen fiber skeleton, human fat stem cells and an extracellular matrix layer, and the extracellular matrix layer is composed of MC3T3‑E1 Cell-guided and secreted matrix proteins and polysaccharides, the extracellular matrix layer covers the surface of the SIS collagen fiber skeleton, and human adipose stem cells adhere to the extracellular matrix layer; the composite engineered bone has high mechanical strength and is highly similar to natural The microstructure of bone can provide good tissue support at the initial stage of implantation in the body, and can provide an excellent osteogenic microenvironment for stem cell implantation. After implantation, human adipose stem cells can rapidly proliferate and differentiate into osteoblasts, and promote the healing of large bone injuries. Rapid repair; in addition, the composite engineered bone has pure natural endogenous characteristics, which can avoid uncontrollable factors caused by the introduction of foreign substances / genes, etc., has good safety, and has broad clinical application prospects in the field of bone regeneration .

Description

technical field [0001] The invention relates to the technical field of tissue engineering and stem cell therapy in biomedicine, in particular to an adipose stem cell-ECM modified SIS composite engineering bone applicable to bone regeneration and a preparation method thereof. Background technique [0002] About 3.5 million people in my country have bone defects due to different reasons every year, and about 1.5 million bone surgery transplants are performed. The demand for replacement bones is very large, especially for replacement bones for large bone injuries. Bone repair in vivo is a synergistic process between cells and scaffold materials. The three-dimensional microstructure of scaffold materials can provide host cells with a good microenvironment for adhesion, proliferation and migration. With the rapid development of tissue engineering technology, the use of tissue engineering principles to combine cells and scaffold materials in vitro and implant them into the body fo...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61L27/38A61L27/36A61L27/24A61L27/50
CPCA61L27/24A61L27/3633A61L27/365A61L27/3834A61L27/3847A61L27/3895A61L27/50A61L2430/02
Inventor 赵基源张迟李梅
Owner 河南源创生命干细胞库科技有限公司
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