Culture method of deciduous tooth pulp stem cells

A dental pulp stem cell and culture method technology, applied in the direction of non-embryonic pluripotent stem cells, animal cells, vertebrate cells, etc., can solve the problems of excessive tissue digestion, poor stability between batches, and unfavorable cells
CN105087474AInactive Publication Date: 2015-11-25GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
GUANGZHOU SALIAI STEMCELL SCI & TECH CO LTD
Publication Date
2015-11-25
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention relates to the field of stem cells and discloses a culture method of deciduous tooth pulp stem cells. The culture method includes: taking pulp tissue, cutting the pulp tissue into pieces, and then adding I-type collagenase; digesting for 10-20min under conditions of 37 DEG C and 200rpm; stopping digestion of a serum-free culture medium; blowing and beating discrete cell mass to obtain single discrete cells; adding cell suspension to re-suspend the cells, and adjusting cell density; culturing the cells in a carbon dioxide incubator at temperature of 37 DEG C and with humidity of 95%; when the cells grow to be fused by 80-90%, using digestive liquid of trypsase to digest the cells before passage culture, wherein the cell suspension is composed of the serum-free culture medium, epidermal growth factor and basic fibroblast growth factor. The cells cultured by the method are good in shape, have tendency of fusiform cluster growing and are high in activity and quick in proliferation, good stem cell characteristics of the deciduous tooth pulp stem cells can be maintained, and the culture method is suitable for large-scale culture of the deciduous tooth pulp stem cells.
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Description

technical field

[0001] The invention relates to the field of stem cells, in particular to a method for culturing dental pulp stem cells, in particular to a method for cultivating dental pulp stem cells in deciduous teeth. Background technique

[0002] In 2003, Miura et al. found a kind of pluripotent stem cells in the dental pulp tissue of exfoliated deciduous teeth. Compared with bone marrow mesenchymal stem cells and permanent tooth pulp stem cells, it has a higher proliferation rate, cell population doubling ability, and clone formation ability. and highly differentiated ability, they were named deciduous dental pulp stem cells (stemcellsfromdeciduousteeth, SHED). Human deciduous tooth pulp stem cells are a kind of mesenchymal stem cells (mesenchymal stem cells, MSCs), mesenchymal stem cells with self-replication ability and multi-lineage differentiation potential, can be induced to differentiate into dentin, osteoblasts, chondrocytes, adipogenic cells Cells, nerve cells...

Claims

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