2results about How to "Meet the basic requirements" patented technology

The present application belongs to the field of microbial application and feed processing technology, and discloses a bacillus subtilis, named bacillus subtilis YT1, which is preserved in China Center for Type Culture Collection, located at Wuhan University, No. 299, Bayi Road, Wuchang District, Wuhan City, Hubei Province, with a preservation number of CCTCC NO: M 20242767 and a preservation date of December 10, 2024. The present application also discloses a microbial preparation and the application of the bacillus subtilis or the microbial preparation in the processing of silage. The present application first simultaneously possesses high efficient lignocellulose degradation ability, excellent fermentation promotion characteristics, significant antioxidant activity and good probiotic characteristics in a strain of bacillus subtilis. This characteristic enables a single bacterial agent to achieve multiple goals such as fiber degradation, fermentation promotion, oxidation protection, which traditionally requires multiple compounded bacterial agents, and fundamentally overcomes the problems of poor compatibility between strains, unstable synergistic effect and high application cost of existing compounded additives.

The invention belongs to the technical field of detection, and particularly relates to a method for determining the content of a glyceryl monostearate polyethylene glycol 100 mixture in a skin external preparation. The method comprises the following steps: respectively taking a proper amount of a glyceryl monostearate-polyethylene glycol 100 mixture reference substance, preparing reference substance solutions with different concentrations by using a diluent, and taking the reference substance solutions as standard curve solutions; taking a proper amount of skin external preparation, shaking and dispersing with a solvent, and treating to obtain a test solution; preparing a series of reference substance solutions, detecting through an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method to respectively obtain peak areas of target peaks in the reference substance solutions, and carrying out standard curve fitting on the peak areas and the concentration of the solutions; and taking the test solution, detecting the test solution by an HPLC-CAD (High Performance Liquid Chromatography-Computer Aided Design) method to obtain the peak area of the test solution, and calculating the content of the glyceryl monostearate-polyethylene glycol 100 mixture in the test solution by a standard curve method. A liquid chromatography-electrospray detector is adopted, and the method is good in specificity, good in linear relation and high in accuracy.