A three-dimensional expansion method with high activity of hepatic stem cells in vitro to maintain cell phenotype
A technology of liver stem cells and high activity, applied in the field of stem cells, can solve the problems of harsh three-dimensional culture conditions, difficult industrialization promotion, high technical requirements, etc., and achieve the effect of good stem cell characteristics, uncomplicated components, and simple culture and expansion methods
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0053] Rat liver stem cell line WB-F344 was purchased from Beijing Yuhengfeng Technology Co., Ltd.
[0054] Prepare medium, which consists of the following components:
[0055] DMEM / F12 1L
[0056] Fibroblast Growth Factor 18 mg / L
[0057] Insulin 13mg / L
[0058] Glutamic acid 0.125mg / L
[0059] Niacinamide 0.7mg / L
[0060] Selenite 0.15mg / L
[0061] β-mercaptoethanol 0.08 mg / L
[0062] α-actin 0.3mg / L
[0063] Sodium acetate 0.25mg / L
[0064] Sodium pyruvate 0.002mg / L
[0065] Fetal bovine serum accounted for 2.5% of the total medium weight.
[0066] Inoculum size 1×10 4 Liver stem cells / ml were seeded in chitosan porous gel with a pore size of 500 microns, placed in the above medium, the volume ratio of the gel to the medium was 2.5:1, at 37°C, the volume fraction was 5% CO 2 Cultured under the conditions of 21 days, the medium was changed on the 5th day of culture, and the medium was changed every 4 days thereafter.
[0067] During the culture process, the LDH c...
PUM
Property | Measurement | Unit |
---|---|---|
pore size | aaaaa | aaaaa |
pore size | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com