5results about How to "Phenotype stable" patented technology

This invention relates to the interdisciplinary field of cell biology, experimental oncology, and translational medicine, specifically to a cancer-associated fibroblast cell line, its construction method, and its applications. This invention fills the research gap in mouse-derived oral squamous cell carcinoma-associated fibroblast cell line models and provides a method for constructing such a cell line. Through the inverted adherent culture and enzymatic digestion methods of this invention, a mouse-derived oral squamous cell carcinoma-associated fibroblast cell line with a clear origin, high purity, and long-term phenotypic and functional stability can be obtained. This allows for the establishment of a cryopreservable and resuscitable mouse oral squamous cell carcinoma-associated fibroblast cell bank, providing technical support for research related to the tumor microenvironment and immunotherapy.

The invention discloses an engineered adoptive macrophage based on a nano battery as well as a preparation method and application thereof, and belongs to the technical field of biological medicines. The preparation method disclosed by the invention comprises the following steps: taking an immunologic adjuvant working solution as a raw material, and obtaining nanoparticles with high drug loading capacity through an assembly effect; as the main energy of ChARM comes from an immunologic adjuvant, the high-drug-loading nano-particle is also called a high-energy-density nano-battery. Diluting the obtained nano particles with a cell culture medium, and co-incubating the diluted nano particles and macrophages to obtain engineered macrophages loaded with the anti-tumor or anti-inflammatory nano battery; the assembly mode is any one of supramolecular assembly, prodrug assembly or metal coordination assembly; the high-density drug-loaded nano battery can endow engineered cells with excellent biological functions. The method solves the technical problems that the preparation of the existing CAR-M is expensive, the gene modification is complex, and the nano-drug delivery system is unstable.

The invention discloses a zhou chicken molecular marker genotype and phenotype correlation analysis and breeding method. The method comprises the following steps: firstly, collecting zhou chicken samples, recording growth, slaughter and reproduction phenotype indexes, extracting genome DNA, screening specific SNP markers on GH, IGF-1 and PRL genes, judging genotypes through PCR amplification and Sanger sequencing, and screening AA (GH), GG (IGF-1) and TT (PRL) dominant genotypes through GLM model correlation analysis; and screening a core parent based on the dominant genotype, and carrying out artificial hybridization, hatching egg incubation, chick breeding, offspring genotype and phenotype double screening and multi-generation breeding to obtain an excellent new strain. According to the new strain, the weight of the 12-week-old cocks is larger than or equal to 1650 g, the weight of the hens is larger than or equal to 1300 g, the slaughter rate is larger than or equal to 88%, the first laying day age of the hens is smaller than or equal to 140 d, the annual egg yield is larger than or equal to 200, the growth speed and the annual egg yield are increased by 25% and 18% respectively compared with a traditional variety, the problems that traditional breeding is long in period and low in accuracy are solved, and accurate and efficient breeding is achieved.

This invention provides a method for constructing a mouse obesity model and its application, relating to the fields of biotechnology and medical animal model technology. This invention is the first to discover and confirm complement. C9 Genetic defects can lead to spontaneous obesity in mice, and based on this, a novel method for constructing an animal model of obesity has been developed. The model constructed using this method exhibits spontaneous obesity even under a normal diet, avoiding the interference of a forced high-fat diet. Under a high-fat diet, the obesity phenotype is accelerated and exacerbated, providing a flexible time window and a stronger phenotype for research. The model constructed using this method can not only be used for obesity research but also extended to the study of its entire spectrum of metabolic complications and the construction of an efficient drug screening platform, possessing significant scientific and commercial value.

The invention relates to the technical field of biology, in particular to a construction method and application of an animal blood stasis model. The nitroglycerin is utilized to successfully induce an animal blood stasis model. Experiments show that blood stasis related phenotypes of zebrafish embryos can be caused by nitroglycerin treatment, for example, blood flow velocity blocking, heart red blood cell dyeing intensity reduction, and blood coagulation factor and endothelial injury marker expression level increase, which indicates that a zebrafish blood stasis model can be successfully established by nitroglycerin treatment.