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Antisense RNA sequence combination and application thereof in production of abortive tilapia

A tilapia and antisense technology, applied in the fields of molecular biology and reproductive biology, can solve the problems of no abortion tilapia, endanger human health, hormone residues, etc., and achieve high success rate, stable representative type, egg small damage effect

Active Publication Date: 2020-11-24
FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, the hormone is easy to remain in the fish body, causing the drug residue to exceed the standard. At the same time, the excess methyltestosterone may be discharged into the water or ingested by the human body, thus endangering human health.
[0003] Currently there is no safe and effective method for aborting tilapia

Method used

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  • Antisense RNA sequence combination and application thereof in production of abortive tilapia
  • Antisense RNA sequence combination and application thereof in production of abortive tilapia
  • Antisense RNA sequence combination and application thereof in production of abortive tilapia

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Embodiment 1

[0056] Step 1, design of the first antisense RNA sequence of SF1-1 (Anti-SF1-1-I): CATCTGGTTCAGTCACTTTGCGTAAGCTGACGTCGTTCATGAACCTACAGACACATACGGCGGTTGAGTGATTTATTATCCTGCGATATGTTTACTTTTAGATTTTTATTTTT (SEQ ID No. 1).

[0057] The antisense RNA contains the first intron partial sequence and the first exon partial sequence plus a few bases of 5-terminal non-translated sequence, which is designed to interfere with the post-transcriptional processing of the SF1-1 gene and interfere with Translation initiation of the gene mRNA.

[0058] The second antisense RNA sequence design (Anti-SF1-1-Ⅱ):

[0059] AATAACTGACAAACTATTCTTAACAATGAAATGTGTTTATAGTTTGTTGTTGTGGTTTTGTTGCTTTCACCTGATCTGACTCGCTGCTGAGTCTCATCTGGTTC (SEQ ID No. 2).

[0060] The antisense RNA comprises the second intron partial sequence and the first exon partial sequence, and its design purpose is to interfere with the post-transcriptional processing of the SF1-1 gene.

[0061] Step 2, the first antisense RNA sequence design of ...

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Abstract

The invention provides an antisense RNA sequence combination and application thereof in production of abortive tilapia, and belongs to molecular biology and breeding biology technologies. The antisense RNA sequence combination comprises antisense RNA of steroid-producing factors SF1-1 and SF1-2; and nucleotide sequences of Anti-SF1-1-II, Anti-SF1-2-I and Anti-SF1-2-II are respectively shown as SEQID No. 1 to SEQ ID No. 4. According to the method, an antisense RNA fragment is introduced into an ovum through a fertilization hole, so that effective and accurate targeted intervention is realizedto regulate expression of genes; and the method is simple to operate, extremely small in ovum damage, high in success rate and stable in representative type after breeding, and has extremely strong application prospect.

Description

technical field [0001] The invention belongs to molecular biology and breeding biology technology, in particular to an antisense RNA sequence combination and its application in producing aborted tilapia. Background technique [0002] Nile tilapia (Oreochromis niloticus) is one of the main freshwater cultured fishes in the world. It has fast growth, strong fecundity and early sexual maturity. Female fish cannot eat during the breeding season and need to consume a lot of energy for breeding needs. At the same time, too many small fish can compete with big fish for feeding, which seriously affects the growth of adult fish. Therefore, there is an urgent need to breed all-male tilapia in production. At present, there are three main methods for breeding all-male tilapia: ①Cross breeding method, which mainly uses the chromosome difference between Nile tilapia and Olia tilapia for hybrid breeding, and the male rate of offspring can reach 85% to 97% . Yet this method is extremely...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/113C12N15/88A01K67/027
CPCC12N15/113C12N15/88A01K67/0275C12N2310/11A01K2207/05A01K2227/40A01K2267/02C12N2310/111A01K67/027
Inventor 强俊曹哲明徐跑何杰刘凯徐刚春李红霞陶易凡朱昊俊
Owner FRESHWATER FISHERIES RES CENT OF CHINESE ACAD OF FISHERY SCI
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