35 results about "Oreochromis" patented technology

The invention discloses a method for improving overwintering survival rate and qualities of oreochromis niloticus fries. Before overwintering, feeds with 12% to 14% fat level are fed to the fries, and soybean oil and fish oil are blent in a proportion of 4:1 to form fat sources. Fatty acids--linoleic acids and linolenic acids which are necessary to growth of oreochromis niloticus are mainly increased so as to improve energy reserve. Low-temperature stress resistance of the oreochromis niloticus can be improved by low temperature acclimatization and the low temperature adaptability is improved. During overwintering, feeds with 1.5% to 2% compound Chinese herbal medicine are fed to the fries, so that damages of the low-temperature stress to fish bodies can be effectively relieved, the non-specificity immunity of the fish bodies is improved, and the overwintering survival rate and fry qualities are improved. Meanwhile, according to the above three steps, material selection and facility configuration are easy, the price is relatively low, processes are simple, traditional problems that overwintering costs are high, the survival rate of the fries is low, and qualities of the fries are poor are excellently solved, and environment-friendly and physically beneficial breeding requirements can be met.

The invention discloses an SNP marker relevant with the growth speed of Oreochromis niloticus and application of the SNP marker. The SNP marker is a basic group G or T at a site 501 from 5' terminal of a nucleotide sequence represented by SEQ ID NO: 1. The SNP marker is closely relevant with the growth speed of the Oreochromis niloticus and can be effectively applied to molecular marker assistant breeding of the Oreochromis niloticus.

The invention discloses a method for improving the streptococcus iniae infection-resisting capacity of GIFT oreochromis niloticus during high-temperature stress. The method comprises the following steps: preparing basic ration and a compound Chinese medicinal herb formula, and feeding feed. Due to the adoption of the method, the immunity index of the GIFT oreochromis niloticus during high-temperature stress can be effectively increased, the liver damage is reduced, meanwhile, the streptococcus iniae infection-resisting capacity of the GIFT oreochromis niloticus can be improved, the morbidity is reduced, and the survival rate of the GIFT oreochromis niloticus infected with streptococcus iniae can be increased. Several Chinese medicinal herbs involved in the method are relatively available and relatively low in cost; a production technology is simple. The basic ration with the compound Chinese medicinal herbs has relatively good palatability, and after the basic ration is taken by the GIFT oreochromis niloticus, drug residues in bodies of the GIFT oreochromis niloticus are relatively few, and therefore, the GIFT oreochromis niloticus meets the requirements on green healthy aquatic products.

The invention belongs to the technical field of genetic engineering and particularly relates to an oreochromis niloticus sex chromosome specific molecular marker, namely an X chromosome specific molecular marker NtX1, which has a nucleotide sequence represented by SEQ ID No.3; through polymerase chain reaction(PCR) amplification, the X chromosome specific molecular marker NtX1 and a Y chromosome specific molecular marker NtY1 and/or a Y chromosome specific molecular marker NtY2 can be used to differentiate YY supermale oreochromis niloticus, XY male oreochromis niloticus and XX female oreochromis niloticus and promote the mass production of all-male oreochromis niloticus fry through a YY supermale oreochromis niloticus breeding route; therefore, the breeding output of oreochromis niloticus is increased obviously, the breeding cost is reduced, and the economic benefit is increased.

The invention discloses a preparation method of feed capable of protecting the liver of GIFT oreochromis niloticus and boosting the immunity of the GIFT oreochromis niloticus and a feeding method of the feed. The preparation method comprises the steps of feed preparation and chitosan addition. The feeding method of feed comprises the step of feed feeding. After the GIFT oreochromis niloticus is fed for 9 weeks by adopting the feeding method, the immunity index of the GIFT oreochromis niloticus is obviously increased, the liver stress is decreased, the oxidative stress resistance capacity of the liver is enhanced, the activity of superoxide dismutase, catalase, glutathione and glutathion peroxidase in the liver is obviously improved, and the level of the malonaldehyde is lowered; the level of glucose, cholesterol and low-density cholesterol in serum is obviously lowered; the level of alkaline phosphatase and high-density cholesterol is obviously increased. The chitosan added to the feed is metabolites generated by fermentation of microorganisms without antagonism and is small in molecular weight, easy to absorb, low in cost and relatively easily available. After the feed is taken by the GIFT oreochromis niloticus, the feed is easy to digest and absorb by the GIFT oreochromis niloticus, and residues are barely remained in bodies; the feed has a very good immunopotentiator effect and meets the requirements on novel green healthy additives.

The invention discloses a compound feed capable of improving the reproductive capacity of Oreochromis niloticus parent fish and a preparation method thereof. The compound feed can obviously improve the reproductive capacity of Oreochromis niloticus parent fish and reinforce the disease resistance. The feed comprises the following ingredients in percentage by weight: fish meal 5-15%, soybean meal 10-30%, dephenolized cottonseed meal 5-15%, double-low rapeseed meal 10-20%, high-gluten flour 10-30%, corn protein powder 5-10%, rice bran 10-20%, phosphatide oil 0.5-1%, soybean oil 1-3%, compound vitamins 0.2-0.5%, compound minerals 1.0-2.0%, phagostimulant 0.3-1.0%, vitamin C phosphate 0.1-0.3%, calcium dihydrogen phosphate 1-3%, anti-mildew agent 0.01-0.05%, Temianhuang 0.2-0.8%, choline chloride 0.05-0.15%, and vitamin E 0.01-0.03%. The feed and the preparation method thereof belong to the technical field of aquatic feeds.

The invention discloses a method for reducing fat deposition in the livers of Oreochromis niloticus during high-density cultivation. The method comprises the following steps: preparation of a basic ration; preparation of a compound Chinese herbal medicine; addition of the compound Chinese herbal medicine; and feeding of fries. Compared with the prior art, the invention has the following advantages: (1) the method provided by the invention can effectively improve the absorption and utilization of fat in feeds, reduce fat deposition in livers, lower the incidence rate of fatty livers and promote aquaculture of healthy Oreochromis niloticus; (2) the compound Chinese herbal medicine used in the method is a green and environment-friendly preparation, is small in residues in Oreochromis niloticus and low in negative effect on environment, and conforms to requirements on green and environmental-friendly feed additives; and (3) with a compound Chinese herbal medicine feed used in the invention, the absorption efficiency of Oreochromis niloticus is good, no residue is left in the body of Oreochromis niloticus, and excrement of the Oreochromis niloticus is free of pollution to environment.

The invention discloses a method for increasing the survival rate of adults of live oreochromis niloticus. The hormone level, the alexin level and the hepatic antioxidant adaptive capacity can be improved within a short time by increasing breeding density and performing tensioning net stress, and nonspecific immunity is improved. The contents of protein, glucose and triglyceride in blood of the oreochromis niloticus can be increased by feeding feedstuff with high protein level of 36%-39% before the oreochromis niloticus is conveyed, and energy reserve is improved. Moreover, 0.5%-0.8% of compound heartleaf houttuynia herb is added in the feedstuff and contains herba houttuyniae, scutellaria baicalensis, radix isatidis, fructus forsythiae, honeysuckle and the like, and the anti-stress capability of the oreochromis niloticus can be improved. In a conveying process, the water temperature is controlled in a range of 19-23 DEG C, the salinity of water is 8%o-12%o, and dissolved oxygen is sufficient. The metabolic activity of the oreochromis niloticus is low, but the immunity of the oreochromis niloticus is high, so that energy loss in the conveying process is reduced, excretion of metabolin is reduced, and the anti-stress capability is improved. After the oreochromis niloticus is treated by using the method, the survival rate of the adults of the live oreochromis niloticus and the live oreochromis niloticus conveying distance are obviously improved during conveyance.

The invention discloses recombinant lactococcus lactis based on the fbsA gene of Streptococcus agalactiae. The recombinant lactococcus lactis comprises the fbsA gene of Streptococcus agalactiae or segments of the fbsA gene, the fbsA gene or the segments of the fbsA gene preferably has (or have) a sequence shown in SEQ ID No. 1, the deposit number of the recombinant lactococcus lactis is CCTCC M 2020140, and expression of recombinant FbsA protein with a theoretical molecular weight of 34.6 kDa can be induced under the culture condition that niusin exists. The invention also provides a vaccine for the Streptococcus agalactiae disease of Oreochromis sp., and the vaccine is prepared from the recombinant lactococcus lactis, and is capable of producing protective antibodies in Oreochromis sp. after Oreochromis sp. is fed with the vaccine in an immune mode of oral gavage, so that Oreochromis sp. is assisted to resist infection of Streptococcus agalactiae effectively.

The invention discloses a method for breeding GIFT, oreochromis niloticus. The breeding method includes the steps that fodder is fed to the GIFT, oreochromis niloticus, the throwing amount of the fodder is 1.5%-3% the body weight of the oreochromis niloticus, and the throwing frequency of the fodder is twice a day; according to the breeding conditions, the water temperature is 30-40 DEG C, the pH value is 7.2-7.8, the dissolved oxygen content is 6-7 mg/L, and the ammonia nitrogen content is smaller than 0.1 mg/L. By the adoption of the breeding method, the fodder throwing amount of the GIFT, oreochromis niloticus can be reduced, meanwhile, the quality of the fed GIFT, oreochromis niloticus is good, and the physiological property is good.

The invention discloses a pond ecological poly-culture method of oreochromis niloticus and macrobrachium rosenbergii. The pond ecological poly-culture method comprises the following steps: step 1, culture pond construction: a plurality of net cages are arranged in a culture pond, have a certain distance from the edge of the culture pond and are not in contact with the bottom of the culture pond; step 2, fry and young shrimp putting: firstly, macrobrachium rosenbergii young shrimps are put in a space outside the net cages in the culture pond, and then, oreochromis niloticus fries are put in the net cages; step 3, feeding management: firstly, macrobrachium rosenbergii feed is fed on the periphery of the culture pond, and then, oreochromis niloticus feed is fed into the net cages. The pond ecological poly-culture method discloses by the invention has the benefits that the farmer breeding cost is reduced, the pond water quality is regulated, and the yield of the oreochromis niloticus and the macrobrachium rosenbergii can also be improved.

Oreochromis niloticus alexin C9 gene clone and application thereof. The invention belongs to functional study of the Oreochromis niloticus infected Streptococcus agalactiae. The invention discloses immune-related gene OnC9 of Oreochromis niloticus, and the nucleotide sequence is shown in the table SEQ ID NO.1. And the invention discloses the sequence of the immune-related protein OnC9 of Oreochromis niloticus, and the amino acid sequence thereof is shown in the sequence table SEQ ID NO.2. The method uses the disclosed nucleotide sequence of Oreochromis niloticus OnC9 to design a primer of fluorogenic quantitative PCR detection (C9-qf:5'-GGCTTGAAGTCGCTCTCCA-3'; C9-qr:5'-GCCAAATGCTTCTACACTTCG-3'), and carries out the fluorogenic quantitative PCR detection for the healthy Oreochromis niloticus or Oreochromis niloticus infected Streptococcus agalactiae. The method can be used for researching immune response of Oreochromis niloticus after infecting Streptococcus agalactiae.

The invention discloses a prevention and treatment method of common diseases of oreochromis mossambila. The prevention and treatment method comprises the steps that a pond is built; healthy fry is selected, and disinfection is carried out; pond disinfection is carried out; the throw-in density of the fry is controlled; a chemical additive is added to feed; pond prevention and treatment are carriedout. The pond is built, and the probability of the diseases of fish bodies is reduced from the source; the health fry are selected, disinfection is carried out, miscellaneous fishes, various spawns,aquatic insects, parasites and pathogenic bacteria can be fast killed in the pond, the reasonable throw-in density is specified, freshness of water and the environmental sanitation of the pond are kept, the diseases are eliminated in time, the high-quality feed is adopted, vitamin B, C and E is added, and different agents are added corresponding to different seasons; meanwhile, a four-fixed mode is used for feeding, the vitality of mycetozoa is reduced, and the diseases are prevented from being generated.

The invention relates to a fish feeder, in particular to an oreochromis sp formula feed, and a preparation method of the oreochromis sp formula feed. The oreochromis sp formula feed comprises the following components in parts by mass: 22 to 31 parts of fish meal, 7 to 15 parts of corn protein powder, 7 to 14 parts of stillage, 4 to 9 parts of bone powder, 6 to 12 parts of herba hyperici japonica, 3 to 7 parts of barbed skullcap herb, 3 to 7 parts of nutgrass galingale rhizome, 2 to 7 parts of pagoda tree flower bud, 2 to 6 parts of Indian kalimeris herb, 1 to 5 parts of platycodon grandiflorum, 1 to 4 parts of broom cypress, 3 to 8 parts of fiddlehead, 2 to 6 parts of fish oil, 1 to 4 parts of honey, 1 to 4 parts of olive oil, and 1 to 4 parts of monocalcium phosphate. The oreochromis sp formula feed has the advantages that after feeding, the coefficient of the oreochromis sp is low; in muscles of the oreochromis sp, the content of crude protein is high, the content of crude fat is low, and the content of flavor amino acid is high.

The present invention describes a process for obtaining extracellular matrix from the skin of tilapia (Oreochromis niloticus) comprising the steps of chemical and enzymatic decellularization, detoxification, chemical disinfection, crosslinking, bleaching, dehydration and sterilization by gamma radiation, more specifically the steps comprised by each of said procedures and use of the extracellular matrix for treating ruptured of tissues, dermatitises, acute, chronic and traumatic lesions, battlefield wounds, necrotic wounds, lacerations, abrasions, bruises and other lesions and conditions. The present invention falls within the fields of pharmacy, medicine and veterinary medicine, dentistry, chemistry, tissue engineering, molecular biology and biotechnology.

The preset invention relates to a promoter to target a fluorescent protein to the muscles of fish, such as A. nigrofasciatus, for ornamental purposes, which is a Mlc3 (myosin, light polypeptide 3, skeletal muscle) promoter. The Mlc3 promoter has the nucleotides of tilapia (Oreochromis niloticus) myosin light chain 3 (Mlc3) promoter region, which is potential to be a tilapia Mlc3 promoter to enhance protein expression in muscle of fish, particularly for the generation of ornamental fish.

Vibrio vulnificus can cause infections in aquaculture-raised fish and is considered an opportunistic human pathogen. We isolated V. vulnificus from diseased hybrid tilapia (Oreochromis niloticus X O. aureus) cultured in a North American water reuse aquaculture facility. We have characterized the isolate using biochemical and molecular methods, developed a disease infection model, and determined that formalin-inactivated whole-cell vaccine provides protection against V. vulnificus. The V. vulnificus isolate was determined to be biotype 1, 16S rRNA type B, vcg type C, and vvhA type 2. Fish vaccinated with the formalin-inactivated whole-cell vaccine responded to vaccination as measured by agglutinating antibody titer. In two separate trials, vaccinated tilapia exhibited relative percent survival of 73 and 60% following challenge with the homologous isolate. In additional trials, vaccinated tilapia exhibited survival values of up to 87.5% following challenge with a heterologous isolate. Use of a mineral oil adjuvant enhanced protection.

The invention discloses a batch breeding method for redhump eartheaters. A soil-based pond with muddy at the bottom is used as a spawning pond, a flat-bottomed ceramic container that is put upside down at the bottom of the pond is used as an egg attaching device, parent fishes are selected and freely bred in a season with a suitable temperature according to a proper male-to-female ratio, fertilized ova are hatched in a shaded aquarium, and fries are cultivated in a fish tank. The batch breeding method has the advantages of high spawning rate, high survival rate in hatching, few fry loss in hatching, high fry yield, simple operation flow and easiness in implementation, and can be applicable to large-scale fry production of oreochromis niloticus and fishes laying eggs on stone plates.