9 results about "Intergenic region" patented technology

The present invention relates to polynucleotides comprising a sequence of a live, infectious, attenuated Flavivirus wherein a nucleotide sequence encoding at least a part of a Filovirus glycoprotein is located at the intergenic region between the E and NS1 gene of said Flavivirus, such that a chimeric virus is expressed, characterised in that the encoded sequence C terminally of the E protein of said Flavivirus and N terminally of the signal peptide of the NS1 protein of said Flavivirus comprises in the following order: a further signal peptide of a Flavivirus NS1 protein, a filovirus glycoprotein wherein the N terminal signal peptide is absent, a TM domain of a flaviviral E protein.

The invention discloses a DNA bar code system and method for identifying glycyrrhiza medicinal plants and hybrid complexes thereof, and belongs to the technical field of molecular biology and traditional Chinese medicine identification. The system is formed by combining three bar codes of a nuclear gene segment ITS, a chloroplast gene segment ndhA and a chloroplast gene spacer trnH-psbA. Wherein ndhA is a core bar code which is screened and verified for the first time, and the specific variation site of the ndhA can be used for accurately distinguishing three kinds of medicinal liquorice, namely, Glycyrrhiza uralensis Fischhex DC., Glycyrrhiza glabra. And Glycyrrhiza inflata Batalin. The system fully utilizes the characteristics of chloroplast paternal heredity of glycyrrhiza, not only can identify homozygous species, but also can efficiently analyze complex hybridization and introgression types, and traces the source of a male parent. Compared with a traditional multi-bar code combination, the method has the advantages of being high in identification success rate (up to 98.08%), easy and convenient to operate and low in cost, and is suitable for liquorice germplasm resource identification, medicinal material market rapid screening and improved variety breeding.

The present application relates to molecular biology technology, specifically to a specific marker of Oplegnathus punctatus recd / mtl5 intergenic region, a detection method and application. The specific marker is a nucleotide sequence of the non-insertion and insertion specific marker of the Oplegnathus punctatus recd / mtl5 intergenic region DNA fragment, wherein the non-insertion sequence is SEQ ID NO: 1, and the insertion sequence is the base shown in SEQ ID NO: 2. Based on the above-mentioned marker, the present application further designs a pair of specific primers: in individuals carrying the DNA insertion variation, two bands of 111 bp and 499 bp can be amplified simultaneously, and the band size difference is 388 bp; in individuals not carrying the insertion variation, only a single band of 111 bp can be amplified. The detection technology has important significance and broad application prospect in the application scenarios of Oplegnathus punctatus gender identification, high-male seed preparation and family selection breeding.

The present application relates to the technical field of molecular biology, in particular to a specific marker of the ccl14 / pnut intergenic region of Oplegnathus fasciatus, a primer pair, a detection method, a kit and application. The specific marker is a nucleotide sequence of the non-insertion and insertion specific marker of the ccl14 / pnut intergenic region DNA fragment of Oplegnathus fasciatus, and the sequence is the base shown in SEQ ID NO: 1 and SEQ ID NO: 2. It is of great significance and application value to carry out female and male gender identification, high-male seed preparation and family selection research based on the ccl14 / pnut intergenic region of Oplegnathus fasciatus.

The present application relates to the field of molecular biology, further to an Oplegnathus punctatus intergenic region specific marker of MPP2 / ELF3 gene, primer pair and application and detection method. The specific marker of the intergenic region insertion variation of the Oplegnathus punctatus MPP2 / ELF3 gene is a DNA fragment specific to the non-insertion and insertion of the intergenic region of the Oplegnathus punctatus MPP2 / ELF3 gene, and the nucleotide sequence of the DNA fragment is shown as SEQ ID NO:1 and SEQ ID NO:2. The present application amplifies 469bp and 185bp bands in individuals with DNA insertion variation by using a pair of primers, and only amplifies 185bp bands in individuals without insertion variation. The detection method of the present application significantly shortens the identification time, improves the detection efficiency, and has important application value in the sex identification and family selection of the offspring of the hybridization between Oplegnathus punctatus males and Oplegnathus stripatus females.

The present invention generally refers to a method of determining the methylation level of a set of biomarkers in a subject suspected of having thyroid cancer, the method comprising: a) from an isolated or extracted genomic DNA obtained from a biological sample from the subject suspected of having thyroid cancer, determining the methylation level of one or more biomarkers selected from the group consisting of cg01378044 (located in TBX18 gene, chr6:85,462,137-85,462,137), cg18305394 (located in the 5' region of PCK1 gene, chr20:56,134,788-56,134,788), cg11925561 (located in an intergenic region of chromosome 5, chr5:3,304,588- 3,304,588), and cg14672100 (located in an intergenic region of chromosome 5, chr5:6,344,259-6,344,259) from the extracted genomic DNA. The present invention also refers to diagnostic, prognostic and patient selection uses of said method.

A nucleic acid molecule in the form of a plasmid comprising an origin of replication, a promoter for the transcription into viral RNA; a 3'NCT triplet upstream of regions for the insertion of gene(s) of interest, separated by Measles virus intergenic regions, and one 5'NCT sharing at least 95% of identity with SEQ ID NO: 11 over its full-length, wherein the 3'NCT triplet shares at least 95% of identity with SEQ ID NO:14 over its full-length, the corresponding helper nucleic acid molecules, a process for the production of virosomes based on these nucleic acid based molecules, the obtained virosomes and their therapeutical uses.

The present invention relates to polynucleotides comprising a sequence of a live, infectious, attenuated Flavivirus wherein a nucleotide sequence encoding at least a part of a arenavirus glycoprotein protein is located at the intergenic region between the E and NS1 gene of said Flavivirus, such that a chimeric virus is expressed, characterised in that the encoded sequence C terminally of the E protein of said Flavivirus and N terminally of the signal peptide of the NS1 protein of said Flavivirus comprises in the following order: —a further signal peptide of a Flavivirus NS1 protein, —an arenavirus Glycoprotein protein lacking the N terminal signal sequence and the GP2 transmembrane domain, —a TM1 and TM2 domain of a flaviviral E protein.

The present application relates to the technical field of molecular biology, and particularly relates to a specific marker of DNA insertion variation in the intergenic region of gins / ndrg4 of Oplegnathus fasciatus, a detection method and application. The specific marker is a nucleotide sequence of the non-insertion and insertion specific marker of the DNA fragment in the intergenic region of gins / ndrg4 of Oplegnathus fasciatus, and the sequence is the base shown in SEQ ID NO:1 and SEQ ID NO:2. Based on the above marker, a pair of special primers is further designed, which can simultaneously amplify two bands of 392bp and 793bp (the difference between the bands is 401bp) in the individual with DNA insertion variation, and only amplify a single band of 392bp in the individual without insertion variation. The detection technology not only significantly shortens the identification period and improves the detection efficiency, but also has important significance and broad application prospect in the application of gender identification of Oplegnathus fasciatus, preparation of high male seedling, family selection and the like.