Quick identification method for paniculate swallowwort root medicinal material and counterfeit species thereof

Abstract

The invention discloses a quick identification method for a paniculate swallowwort root medicinal material and counterfeit species thereof, namely rhizoma cynanchi stauntonii and blackend swallowwortroots. The quick identification method comprises the following steps: 1) extracting DNA of a to-be-detected sample; 2) taking the DNA of the to-be-detected sample as a template, and performing PCR amplification on a fragment of an ITS2 sequence containing ribosomal DNA; 3) splicing amplification products to obtain a complete ITS2 intergenic region; 4) analyzing interspecific variation sites of paniculate swallowwort root and counterfeit species thereof, namely rhizoma cynanchi stauntonii and blackend swallowwort roots, identifying as paniculate swallowwort roots if the 50-th site is a basic group C, and identifying as counterfeit species of paniculate swallowwort roots if the 50-th site is basic group deletion. The quick identification method is strong in applicability, is simple to operate, can realize quick and accurate identification for the paniculate swallowwort root medicinal material and counterfeit species thereof, and can identify a great number of samples within a short time.

Description

technical field [0001] The invention belongs to the technical field of identification of source varieties of traditional Chinese medicinal materials, and in particular relates to a rapid identification method of Xu Changqing medicinal material and its counterfeit products. Background technique [0002] The source of Xu Changqing is the dried roots and rhizomes of Cynanchum paniculatum, a plant of the genus Cynanchum in the Ascaridaceae family. It has a long history of medicinal use and has good pharmacological effects such as lowering blood pressure, lowering blood fat, anti-tumor, and anti-cancer. The 2015 edition of the Pharmacopoeia of the People's Republic of China contains 3 kinds of traditional Chinese medicines derived from 5 species of Vinerum genus: Xu Changqing, Baiqian and Baiwei. Among them, Baiqian comes from the dry rhizome and root of Cynanchum stauntonii or Cynanchum glaucescens of Astragalus; Baiwei comes from the dry root and rhizome of Cynanchum atratum or...

AI Technical Summary

Problems solved by technology

Due to the similarity in shape and traits, there are often situations of confusion, indistinguishability, misharvesting, misharvesting and misuse in the market, and Baiqian and Baiwei have records of reverse misuse since ancient times, and the phenomenon of mixed use and misuse still exists today. And people who use cheap Bai Wei to pretend to be Xu Changqing also appear from time to time

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