Method for identifying honeysuckle and lonicera confuse and application of same

Abstract

The invention relates to a method for identifying honeysuckle and lonicera confuse and an application of the method. The method disclosed by the invention comprises the step of performing PCR (polymerase chain reaction) amplification on ITS2 nucleotide sequence, and particularly comprises the steps of 1) extracting the DNA (deoxyribonucleic acid) of a sample; 2) performing PCR amplification on the segments of the ITS2 sequence containing ribosome DNA; 3) splicing the amplification products to obtain a complete ITS2 intergenic region; and 4) establishing the NJ (neighbour-joining) tree of the ITS2 sequence of the sample. The method disclosed by the invention can be used for effectively solving the problems of variety identification, variety improvement and breeding of honeysuckle medicinal materials, development and utilization of germplasm resources, and the like; and the method disclosed by the invention is wide in applicability, simple to operate, easy to grasp, high in accuracy, and capable of successfully realizing rapid and accurate identification on honeysuckle medicinal materials or powder and lonicera confuse medicinal materials or powder.

Description

Technical field [0001] The invention relates to the technical field of identification of the source varieties of Chinese medicinal materials, in particular to a method and application for identifying honeysuckle and Lonicera japonica medicinal materials by using ITS2 fragments of ribosomal DNA. Background technique [0002] Honeysuckle (Flos Lonicerae Japonica) is the dry bud or the first blooming flower of Lonicera japonica Thunb. Flos Lonicerae is Lonicera macranthoides Hand.-Mazz. , Lonicera hypoglauca Miq. Lonicera confusa DC. Lonicera fulvotomentosa Hsu et S. Lonicera confusa DC. C. Cheng's dry buds or first-opening flowers. Honeysuckle and Lonicera japonica are both commonly used precious Chinese medicinal materials. They are widely used in clinical practice. They have the effects of clearing away heat and detoxification, and dispersing wind-heat. However, the two are different in the content of medicinal ingredients and their effects are also focused. Their traits are ...

AI Technical Summary

Problems solved by technology

Both honeysuckle and mountain silver flower are commonly used precious Chinese herbal medicines, which are widely used clinically. They have the effects of clearing away heat and detoxification, and evacuating wind-heat. Their properties are very similar, and it is extremely difficult to distinguish the two by traditional methods. The identification method used in the Pharmacopoeia has a certain degree of subjectivity, and must require that the medicinal materials have a complete shape, no damage, and poor operability. Requires extensive professional knowledge and identification experience

At present, the mixed use of honeysuckle and mountain silver flower has appeared in the market, which affects the quality and safety of the drug to a certain extent.

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