ITS fragment of skunk bush ribosome DNA and method for preparing same

Abstract

The invention relates to an ITS fragment of a dogwood ribosomal DNA and a method for producing the same, effectively solving the problems of variety identification, variety improvement, breeding and exploitation and use of germplasm resources. The ITS fragment consists of three parts which are ITS1, 5.8 S, and ITS2. The method comprises the following steps of: grinding leaves of a dogwood tree; adding PVP powder into the ground leaves and grinding the mixture into fine powder; placing the fine powder into an extraction buffer solution for configuration; removing supernatant; adding beta-Mercaptoethanol and extract made of Vc+2xCTAB into the residuum and mixing the mixture uniformly; adding a solution made of saturated phenol chloroform isoamyl alcohol twice at the same volume; removing impurities and configuring; getting supernatant and adding isopropyl alcohol into the supernatant; collecting sedimentations; and draining off alcohol, and thus obtaining the DNA; carrying out PCR amplification and purification to the DNA by a primer; and sequencing the purified PCR resultant to obtain the ITS fragment. The method is simple, scientific, economic and excellent in social effect.

Description

1. Technical Field [0001] The invention relates to the field of medicine, in particular to an ITS fragment of Cornus officinalis ribosomal DNA and a preparation method thereof. 2. Background technology [0002] Cornus, also known as cornus, cornus, jujube, jujube skin, etc., is a cornus plant Cornusofficinalis Sieb.et Zucc, the dried and mature pulp removed from the pit. It is a rare Chinese medicinal material in China. It has nourishing liver and kidney and astringent essence. The effect of solid off, mainly treats dizziness, tinnitus, waist and knee pain, impotence and nocturnal emission, enuresis, frequent urination, uterine bleeding, profuse collapse, internal heat and thirst, etc. Modern research has proved that: dogwood contains a variety of ingredients, such as organic acids, Iridoid glycosides, saponins, tannins, polysaccharides, amino acids, vitamins and minerals, etc., have a good function of regulating the immune system, lowering blood sugar, anti-inflammatory, and ant...

AI Technical Summary

Problems solved by technology

With the adjustment of the planting structure in rural areas in my country in recent years, the cultivation area of ​​dogwood is gradually expanding. Due to the uneven quality of dogwood fruit trees, the yield varies, and the quality varies greatly, which has seriously affected the production development and clinical application of dogwood.

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