41 results about "Lonicera macranthoides" patented technology

The preparation method of chlorogenic acid and isochlorogenic acid compound uses leaf of lonicera macranthoides as raw material and adopts the following steps: defatting, using ethyl alcohol with a certain concentration to make extraction, concentrating ethyl alcohol extract, feeding the concentrated ethyl alcohol extract into macroporous resin column, eluting by using 0-10% ethyl alcohol to obtain chlorogenic acid and its isomer, then eluting with 20-35% ethyl alcohol to obtain isochlorogenic acid and its isomer; mixing then according to different ratio and making treatment so as to obtain total phenelic acid, its content can be up to 80%.

The invention discloses a breeding method of tissue culture seedling of lonicera macranthoides Yulei No.1, comprising the following steps of: taking lamina extracted in the current year as explant, sterilizing the lamina and inoculating the lamina on an induced medium to culture callus in an inducing way, transplanting the callus into a differential medium to carry out the differential cultivation of adventitious bud, cutting the obtained primary cluster buds into single buds and transplanting the single buds into a subculture medium to carry out the subculture of adventitious buds, when the sub-cultured cluster buds grow to the height of 1.5-2cm, cutting the sub-cultured cluster buds into single buds and transplanting the single buds into a rooting medium to culture in a rooting way, andtransplanting generated test-tube plantlet into a pearlite-peat soil mixed matrix with the volume ratio of 1:9 to culture, to obtain the tissue culture seedling of lonicera macranthoides Yulei No.1. According to the method, the induced medium, the differential medium, the subculture medium and the rooting medium can be optimized. The breeding method has the advantages of being high in callus induction rate, high in adventitious bud differentiation rate and multiplication coefficient, high in rooting rate, high in test-tube plantlet transplanting survival rate, robust in sprout growth, normal in lamina shape and color, subcutaneous in rooting, quick in the germination of new leaves and the like, so that the requirement of the large-area and large-scale cultivation of the lonicera macranthoides Yulei No.1 can be met.

The invention discloses triploid lonicera confusa and a cultivation method thereof. The cultivation method comprises the following steps: taking grown-up plants of diploid lonicera hypoglauca miq and lonicera macranthoides as original parents, planting the parents according to the conventional technology, and then mutually pollinating corresponding plants in a hybridization manner; inducing the parents to form diploid pollen when the parents just enter a green bud period; picking the induced flower in a white bud period, taking out and drying anther, and then screening the diploid pollen for low temperature storage; hybridizing the diploid pollen with haploid oocytes of corresponding plants to obtain hybrid seeds; breeding the hybrid seeds into complete plants and then screening triploid plants; and planting the triploid plant according to the conventional technology, and budding to obtain triploid lonicera confusa. Compared with the prior art, by adopting the method, the diploid pollen can be screened more quickly, the difficulty of sterility in a hybridization period can be solved, the breeding time can be obviously shortened, the operation is simple and feasible, the genetic advantages of the triploid lonicera confusa are higher than those of diploid or tetraploid plants, and the planting economic benefit is high.

The invention discloses a tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a callus induction medium which comprises B5, 2mg/L of 6-BA (6-benzylaminopurine), 2mg/L of KT (kinetin), 0.1mg/L of NAA (naphthylacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud differentiation medium which comprises B5, 1mg/L of 6-BA, 2mg/L of KT, 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud subculture medium which comprises MB, 1mg/L of 6-BA, 0.8mg/L of IAA (indoleacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1/3MB, 1.5mg/L of IBA (indolebutyric acid), 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 20g/L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious bud differentiation rate and propagation coefficient, high rooting rate, high transplanting survival rate of test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf germination, and the like, can satisfy needs of the large-scale cultivation of Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.

The invention provides an extraction method and application of total flavone extract from lonicera macranthoides leaves. The total flavone of lonicera macranthoides leaves is furthest extracted by adopting a water-extraction and alcohol precipitation process; and the flavone compound is selectively separated through macroporous resin, and is concentrated and dried to obtain the flavone compound with high purity. The process is simple, and is suitable for large-scale industrial production, and the total flavone content of the total flavone extract prepared from the lonicera macranthoides leaves is higher than 50 percent. The total flavone extract has excellent activity in treating autoimmune hepatitis, hyperglycemia and hyperlipidemia syndromes.

The invention relates to a method for preparing Lonicera macranthoides saponin and its application in treating liver cancer, breast cancer and carvical carcinoma. Said method comprises following steps: extracting Lonicera macranthoides and bud with ethanol, decoloring with active carbon, grading in macroreticular resinous column or polyamide column, washing with ethanol, basic hydrolyzing, decoloring with active carbon for the second time, crystallizing and getting said product. The invention is characterized by high productivity and purity of effective component, which is about 98%, and is especially for large- scale production. The effective dosage of Lonicera macranthoides saponin is toxic to external cancer cell, and inhibitive to mouse liver cancer H22, mouse S180 cancer and Lewis lung cancer.

The invention relates to lonicera japonica thunb tea and a preparation method thereof. The lonicera japonica thunb tea is prepared from the sprout of lonicera japonica thunb or the sprout of lonicera macranthoides hand by the steps of picking fresh sprout, naturally withering, removing green with steam, spreading, airing, shaping and drying. The lonicera japonica thunb tea provided by the invention has a dense slim cord shape, emerald green color and pure aroma, and is brewed with boiled water to drink; and the liquor color is light green, the aroma is pure, and the mouthfeel is fragrant and refreshing. The product contains tea polyphenol, chlorogenic acid, total flavonoids, vitamins, chlorophyll, carotenoids, multiple amino acids and multiple trace elements, and has certain effects of inhibiting bacteria, easing pain, resisting inflammation and clearing heat. The processing method is simple, and the cost is low.

The invention provides a special fertilizer for Lonicera macranthoides, which is characterized by comprising the following components in percentage by weight: 50.0-70.0% of composted chicken manure or any other poultry or livestock manure (by dry matter weight), 25.0-45.0% of inorganic fertilizer (by dry matter weight), and filler. The invention also discloses an application method of the special fertilizer for Lonicera macranthoides. The special fertilizer for Lonicera macranthoides has the advantages of high harmless degree, high organic content and abundant nutrient substances, and is beneficial to growth and ripening of Lonicera macranthoides.

The invention relates to an ethyl acetate extract of Lonicera macranthoides, a preparation method and application thereof. The extract is prepared by the method of: soaking Lonicera macranthoides by petroleum ether, then conducting percolation by petroleum ether, recovering the residue, volatilize the solvent in the residue, subjecting the residue to reflux extraction by ethanol, concentrating the extracted solution, then performing extraction with ethyl acetate, and collecting the supernatant, thus obtaining the ethyl acetate extract of Lonicera macranthoides. In vitro tests find that the ethyl acetate extract of Lonicera macranthoides provided by the invention can inhibit EGFR kinase activity, has obvious effect, and can resist skin cancer, leukemia, colon cancer, liver cancer, gastric cancer, breast cancer and other tumors.

The invention discloses a method for germination of lonicera macranthoides seeds. The method comprises the following concrete steps: soaking complete and full lonicera macranthoides seeds in a 100-500mu g/ml carbendazim or 500-1000mu g/ml mildothane solution for 12-24h, flushing with clear water, then putting in 4-DEG C wet river sand subjected to sterilization treatment, covering sand layers more than 5cm for carrying out wet sand lamination for 20-100 days, and then putting at 5-20 DEG C for illumination or dark germination. The method disclosed by the invention is simple, the germination ratio of the seeds is more than 60% by controlling the disinfection method, lamination time, germination temperature and illumination, the germination period is short, the decay proportion is low, the accuracy ratio of a germination test is high, and the problems of long dormancy period and difficult germination test of lonicera macranthoides are basically solved.

The invention discloses a twig cutting seedling breeding method for lonicera macranthoides. The method comprises the steps that (1) a disposable plastic cup and a disposable plastic bag are prepared, a hole is cut in the bottom of the disposable plastic cup, and a hole is cut in the plastic bag for subsequent use; (2) a matrix is put into the disposable plastic cup; (3) the opening of the disposable cup is wrapped by the plastic bag and then firmly tied, and the hole of the plastic bag is located at the center of the opening of the disposable plastic cup; and (4) a lonicera macranthoides cutting is inserted via the hole of the plastic bag into the matrix for seedling breeding. The method disclosed by the invention effectively solves the current problem of cutting rotting during twig cutting rooting of old lonicera macranthoides and can be applied in the large-area twig cutting seedling breeding of the lonicera macranthoides. Generalization and application of the method disclosed by the invention can greatly enhance a supply capability of lonicera macranthoides improved seedlings and can obtain huge economic benefits.

The invention brings forward a method for detecting storage condition of a lonicera macranthoides bud medicinal material. The method comprises the following steps: respectively measuring the color of lonicera macranthoides bud and content of six components of chlorogenic acid, caffeic acid, 3,5-dicaffeoylquinic acid, galuteolin, lonicera macranthoides saponins ethyl and Dipsacus asper wall saponins ethyl in lonicera confuse before and after the storage, combining changes of appearance colour and change degree of six components content before and after the storage, and evaluating whether the storage condition of the lonicera macranthoides bud is appropriate or not. The method is scientific and simple, and is in favor of guarantee of medicinal material quality, and fills the blank that no appropriate detection and evaluation method is existed for detecting storage condition of lonicera macranthoides bud.

The invention belongs to the technical fields of tree secondary metabolism active ingredients and natural medicines, and particularly relates to a method for preparing macranthoin G by taking medicinal tree seed eucommia ulmoides as a raw material, and use of the macranthoin G in preparation of a neuroprotective medicine. The macranthoin G is prepared from eucommia ulmoides tree plant materials as raw materials in manners of drawing, extracting and separating. Themacranthoin G is simple in preparation technology, low in cost, short in cycle and high in yield. An experiment proves that themacranthoin G plays a significant protection role on a model cell injury, and indicates that the macranthoin G can be applied to preparation of the neuroprotective medicine, so as to prevent and treat various neurodegenerative diseases.

The invention discloses an efficient and simple method for separating macranthoidin from lonicerae flos. The method comprises the following steps of: a, soaking and extracting lonicerae flos material, using saturated lime water, the amount of which is 5-8 times that of the lonicerae flos material adjusting the extract liquor to neutral, adding to an ultra-filtration membrane for carrying out ultra-filtration after filtering, concentrating permeate by a sodium filtration membrane, adding alcohol to the concentrated solution for precipitating and centrifuging, and concentrating the liquid under reduced pressure to obtain extract; and b, purifying the extract by adopting high-speed counter-current chromatography, carrying out online monitoring by an ultraviolet detector, collecting target components according to a graph, recycling the reagent, and drying under reduced pressure to obtain the macranthoidin. The method for producing macranthoidin is adopted, so that the process is simple, the yield is high, the pollution is small and the industrial expansion is easy.

The invention relates to the field of natural products, and in particular to a novel compound (lonimacranthoidin A) extracted and separated with the radix lonicerae macranthoides as a material by a chemical method for natural products, the framework of which belongs to syn-pimarane diterpenoid. The compound has a higher inhibiting effect on agricultural pathogenic fungi, and can be used in the preparation of chemicals for resisting agricultural pathogenic fungi.

The invention relates to a traditional Chinese medicinal composition for yin nourishing and pathogenic fire reducing, and its application. The traditional Chinese medicinal composition comprises, by weight, 5-15 parts of Radix Scrophulariae, 10-70 parts of Lonicera macranthoides, 5-15 parts of unprepared rehmannia root, 10-25 parts of Radix Puerariae, 5-15 parts of licorice root and 5-20 parts of Stevia Rebaudiana Bertoni. A method for processing the composition to prepare a preparation or a healthcare product mainly comprises the steps of water decoction or extraction, centrifugation, ultrafiltration, membrane concentration and the like. The traditional Chinese medicinal composition cooperatively utilizes the pharmacological properties of the above traditional Chinese medicinal materials, and has the efficacies of heat clearing, detoxifying, and heatstroke, cold and enteritis preventing and curing, so the composition can effectively absorb the benefiting and maintenance of nutritional and greasy products; and a beverage prepared by using the composition has the characteristics of fresh and sweet taste, abundant nutrition, no toxic side effects, and development of the application of the composition in the field of healthcare products.

The invention provides a method for identifying lonicera confusa with different base sources, which comprises the following steps: taking neochlorogenic acid, chlorogenic acid, 3, 5-O-dicaffeoylquinic acid, 4, 5-O-dicaffeoylquinic acid, lonicera macranthoides saponin B, dipsacus asper saponin B, cryptochlorogenic acid, isochlorogenic acid B, secoxyloganin and lonicera macranthoides saponin A reference substances; preparing a mixed reference substance solution; the method comprises the following steps: respectively preparing test sample solutions of different original lonicerae flos from samples of different original lonicerae flos; respectively carrying out ultra-high performance liquid chromatography analysis on the mixed reference substance solution and the test solutions of the different original lonicerae flos, and respectively constructing characteristic spectrums of the different original lonicerae flos; and taking an object to be detected, carrying out ultra-high performance liquid chromatography analysis, and comparing the obtained spectrum of the object to be detected with the characteristic spectrum of the lonicera confusa with different bases. The identification method has the advantages of being simple to operate, rich in map information, strong in characteristic and good in reproducibility.

The invention discloses a method and culture medium for obtaining lonicera macranthoides regeneration plants through tissue culture. The culture medium for obtaining the lonicera macranthoides regeneration plants is prepared from, by mass, 1,650 parts of major elements (metered by NH4NO3), 0.83 part of trace elements (metered by KI), 37.3 parts of ferric salt (metered by FeSO4.7H2O), 0.5 part of organic ingredients (), 0.1-1.5 parts of zeatin, 0.1-0.5 part of indolebutyric acid and 30,000 parts of saccharose. The differential culture medium for obtaining the lonicera macranthoides regeneration plants is high in pertinence and good in applicability. The method for obtaining the lonicera macranthoides regeneration plants through the tissue culture has the advantages of being not limited by regions, weather and seasons and being beneficial for massively producing single plants with the fine properties.