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New honeysuckle lonimacranthoide and preparation method and application thereof

A technology of sulfate esters and saponins, which is applied in the field of natural medicinal chemistry and can solve problems such as adverse reactions

Active Publication Date: 2014-07-02
河南金陵金银花药业有限公司 +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although most patients respond well to immunosuppressants, long-term application has certain adverse reactions, and the recurrence rate after drug withdrawal is as high as 80%

Method used

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  • New honeysuckle lonimacranthoide and preparation method and application thereof
  • New honeysuckle lonimacranthoide and preparation method and application thereof
  • New honeysuckle lonimacranthoide and preparation method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1. Isolation and identification of the compound Lonimacranthoide V from Lonicera cinerea

[0022] 10 kg of dried flower buds of Lonicerae pilosula were extracted with 100 kg of 95% (volume ratio) ethanol-water solution under reflux for 3 times, each time for 2 hours, and concentrated until there was no alcohol smell to obtain an extract (dry weight 1.7 kg); the obtained extract was added 10 times volume of water dissolved, filtered through filter paper to remove water-insoluble matter, the filtrate was adsorbed with macroporous resin HP-20, then eluted with water, 20%, 70% ethanol solution, combined with 70% ethanol solution eluate, concentrated to obtain gray felt Total saponins of Lonicerae pubescens 860g. The obtained total saponins of Lonicera tomentosa were subjected to silica gel column chromatography, and the eluent was chloroform-methanol-water (17:3:0.2→4:1:0.1→7:3:0.5→3:3:0.5), methanol Wherein chloroform-methanol-water (3: 3: 0.5) part carries out re...

Embodiment 2

[0025] Embodiment 2, water extraction method prepares Lonimacranthoide V from the gray felt wool honeysuckle

[0026] 1 kg of dried flower buds of Lonicerae pilosula, heated and extracted three times with water, the water consumption was 10 liters, the extraction time was 2 hours, the extraction temperature was 60°C, the extract was absorbed by macroporous resin D101, and then washed with water, 15%, 75% ethanol Take off, and 75% ethanol eluent recovers solvent under reduced pressure to obtain 87g of total saponins of Lonicera tomentosa. Gained total saponins of Lonicerae Lonicerae carry out silica gel column layer

[0027] Table 1

[0028] 1 H(500M Hz)and 13 C NMR(125M Hz)spectral data for Lonimacranthoide V in pyridine-d 5

[0029]

[0030] For analysis, the mobile phase was chloroform-methanol-water (17:3:0.2→4:1:0.1→7:3:0.5→3:3:0.5), methanol in turn; chloroform-methanol-water (3:3: 0.5) Repeated reverse-phase silica gel C-8 column chromatography (eluent is 30% et...

Embodiment 3

[0031] Embodiment 3, prepare Lonimacranthoide V from the gray felt wool honeysuckle with methanol cold soaking extraction method

[0032] 1Kg of dried flower buds of Lonicerae pilosula, extracted three times by cold immersion in methanol, the amount of methanol used was 20 liters, the extraction time was 20 days, the extraction temperature was room temperature, and the extract was concentrated to an alcohol-free extract (dry weight was 120g); Add 10 times the volume of water to dissolve the extract, filter paper to remove water-insoluble matter, absorb the filtrate with macroporous resin AB-8, then elute with water, 10%, 80% ethanol solution, and combine the 80% ethanol solution eluent , and concentrated to obtain 81g of total saponins of Lonicerae cinerea. Gained total saponins of Lonicera tomentosa are subjected to column chromatography (silica gel column chromatography: chloroform-methanol-water gradient elution system, RP-C18 column chromatography: water-methanol system, g...

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Abstract

The invention relates to the field of natural medicinal chemistry, and discloses a chemical structure of a new honeysuckle lonimacranthoide compound extracted and separated from lonicera macranthoides buds. The compound has the structure of the right formula. The invention also discloses a preparation method of the compound and application of the compound in the field of medicine, particularly application in immune hepatic injury.

Description

1. Technical field: [0001] The invention relates to the field of natural medicinal chemistry, and discloses a new honeysuckle sulfate saponin compound extracted and separated from the flower buds of Lonicerae cinerea, as well as the preparation method of the compound and its application in the field of medicine, especially in the preparation and treatment of autoimmunity The use of liver injury drugs. 2. Technical background: [0002] Lonicera macranthoides Hand.-Mazz. is a plant of Lonicera genus Lonicera. It has the effects of clearing away heat and detoxifying, antibacterial and anti-inflammatory. Dysentery, Fengreganmao, febrile disease and other diseases treatment. It was included in the 2005 edition of "Chinese Pharmacopoeia", and it was listed under the mountain silver flower together with red glandular honeysuckle and South China honeysuckle. The Institute of Botany, Chinese Academy of Sciences, Jiangsu Province, obtained a new sulfate saponin compound named Lonima...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J63/00C07H15/256C07H1/08A61K31/704A61P37/00A61P1/16
Inventor 陈雨冯煦王鸣赵友谊王奇志单宇孙浩管福琴
Owner 河南金陵金银花药业有限公司
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