Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts

A tissue culture medium and a culture medium technology, applied in the field of plant tissue culture, can solve the problems of inability to meet production and market demands, variation in plant traits, and low survival rate of cuttings, and achieve good application prospects, high rooting rate, and high survival rate. Effect

Inactive Publication Date: 2012-07-18
CHONGQING UNIV OF ARTS & SCI
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Problems solved by technology

Due to the large variation in plant traits of this variety, clonal breeding methods such as cuttings and grafting are usually used to propagate excellent single-plant clones. However, there are

Method used

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  • Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts
  • Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts
  • Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts

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Embodiment Construction

[0024] In order to make the purpose, technical solution and advantages of the present invention clearer, the present invention will be further described in detail below in conjunction with the accompanying drawings. The explant No. 1 of Lonicerae cinerea Yulei used in the examples was provided by the greenhouse of the Flower Research Institute of Chongqing University of Arts and Science.

[0025] 1. Optimization of callus induction medium

[0026] Rinse the No. 1 explant of Lonicerae pilosula Yulei (the leaves extracted in the same year) with tap water, rinse with 70% ethanol solution for 30 seconds, and then rinse with 0.1% HgCl 2 Disinfect the solution for 8 minutes, and finally rinse with sterile water 4 to 5 times to thoroughly wash away residual HgCl 2 . Cut the sterilized leaves into 1cm 3 size, inoculated on callus induction medium. The callus induction medium used MS, MB and B5 as the basic medium, and added 6-BA 1mg / L, KT 2mg / L, NAA 0.1mg / L, carrageenan 6g / L and s...

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Abstract

The invention discloses a tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a callus induction medium which comprises B5, 2mg/L of 6-BA (6-benzylaminopurine), 2mg/L of KT (kinetin), 0.1mg/L of NAA (naphthylacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud differentiation medium which comprises B5, 1mg/L of 6-BA, 2mg/L of KT, 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; an adventitious bud subculture medium which comprises MB, 1mg/L of 6-BA, 0.8mg/L of IAA (indoleacetic acid), 5.5-6.0g/L of carragheenan and 30g/L of sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1/3MB, 1.5mg/L of IBA (indolebutyric acid), 0.1mg/L of NAA, 5.5-6.0g/L of carragheenan and 20g/L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious bud differentiation rate and propagation coefficient, high rooting rate, high transplanting survival rate of test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf germination, and the like, can satisfy needs of the large-scale cultivation of Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and relates to a tissue culture medium for plant seedlings. Background technique [0002] Gray felted honeysuckle (Lonicera m acranthoides Hand. Mazz) is a perennial semi-evergreen winding small shrub or erect small shrub plant of Lonicera genus Lonicera. The content of chlorogenic acid in its flower buds is the highest among all kinds of honeysuckle in the country. One of the main varieties of commercial honeysuckle. [0003] Yulei No. 1 was approved by the Chongqing Forest Variety Approval Committee in 2008 as a new variety of Lonicera aureus. The flower yield of this variety is more than 400 kg per mu, which is 30-80% higher than that of the traditional planting of Lonicera chinensis. Its stress resistance and adaptability are also stronger than that of traditional home-grown Lonicera spp. ~30 days and the entire flowering period is full of flower buds, basically no flowering. Co...

Claims

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Application Information

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IPC IPC(8): A01H4/00
Inventor 陈泽雄刘奕清廖林正黄登艳唐建民黄科
Owner CHONGQING UNIV OF ARTS & SCI
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