Tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts

Abstract

The invention discloses a tissue medium for Lonicera macranthoides Hand. Mazz Yulei No.1 sprouts. The medium comprises at least one of the following mediums: a callus induction medium which comprises B5, 2mg / L of 6-BA (6-benzylaminopurine), 2mg / L of KT (kinetin), 0.1mg / L of NAA (naphthylacetic acid), 5.5-6.0g / L of carragheenan and 30g / L of sucrose and has a pH value of 5.6-5.8; an adventitious bud differentiation medium which comprises B5, 1mg / L of 6-BA, 2mg / L of KT, 0.1mg / L of NAA, 5.5-6.0g / L of carragheenan and 30g / L of sucrose and has a pH value of 5.6-5.8; an adventitious bud subculture medium which comprises MB, 1mg / L of 6-BA, 0.8mg / L of IAA (indoleacetic acid), 5.5-6.0g / L of carragheenan and 30g / L of sucrose and has a pH value of 5.6-5.8; and a rooting medium which comprises 1 / 3MB, 1.5mg / L of IBA (indolebutyric acid), 0.1mg / L of NAA, 5.5-6.0g / L of carragheenan and 20g / L of sucrose and has a pH value of 5.6-5.8. The medium of the invention, which has the advantages of high callusinductivity, high dventitious bud differentiation rate and propagation coefficient, high rooting rate, high transplanting survival rate of test tube sprouts, robust sprout growth, normal leaf shape and leaf color, rapid subcutaneous rooting and new leaf germination, and the like, can satisfy needs of the large-scale cultivation of Lonicera macranthoides Hand. Mazz Yulei No.1, and has a good application prospect.

Description

technical field [0001] The invention belongs to the technical field of plant tissue culture and relates to a tissue culture medium for plant seedlings. Background technique [0002] Gray felted honeysuckle (Lonicera m acranthoides Hand. Mazz) is a perennial semi-evergreen winding small shrub or erect small shrub plant of Lonicera genus Lonicera. The content of chlorogenic acid in its flower buds is the highest among all kinds of honeysuckle in the country. One of the main varieties of commercial honeysuckle. [0003] Yulei No. 1 was approved by the Chongqing Forest Variety Approval Committee in 2008 as a new variety of Lonicera aureus. The flower yield of this variety is more than 400 kg per mu, which is 30-80% higher than that of the traditional planting of Lonicera chinensis. Its stress resistance and adaptability are also stronger than that of traditional home-grown Lonicera spp. ~30 days and the entire flowering period is full of flower buds, basically no flowering. Co...

AI Technical Summary

Problems solved by technology

Due to the large variation in plant traits of this variety, clonal breeding methods such as cuttings and grafting are usually used to propagate excellent single-plant clones. However, there are not many rootstocks and scion resources for grafting, and the survival rate of cuttings is lower than 40%, resulting in a low reproductive coefficient. , cannot meet production and market demand

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