Tissue culture method of lycoris incarnata

A culturing method and tissue culturing technology are applied in the field of tissue culture of lycorale, which can solve the problems of insufficient number of bulbs, lag in garden application, limited distribution area, etc., and achieves efficient propagation, improved callus induction rate, and protection and safety. Effect

Active Publication Date: 2019-07-09
INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, due to its limited distribution area, the small number of bulbs and the inability to reproduce by seeds, the garden application of this excellent variety lags behind.

Method used

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  • Tissue culture method of lycoris incarnata
  • Tissue culture method of lycoris incarnata
  • Tissue culture method of lycoris incarnata

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Experimental program
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Embodiment 1

[0035] The high-efficiency tissue culture method of Lycoris provided by the invention adopts multi-hormone combination technology:

[0036] (1) Cultivate clustered buds from the bulbs of Lycoris

[0037] Take 2 healthy lycoris bulbs, clean them, remove the root tissue and the outer brown cortex with a knife, cut off the upper 2 / 3 of the bulb tissue, and keep 1 / 3 of the base of the bulb disc. Then carefully remove the 2-3 layers of scales outside the bulb, and then cut the bulb along the middle and divide it into 2 small pieces. Rinse the cleaned small pieces under running water for 30 minutes, soak in 0.1% Tween 20 aqueous solution for 30 minutes, rinse with clean water 2 to 3 times, transfer to the ultra-clean workbench, and perform the following sterilization process: 75% alcohol, 30s; 4 % sodium hypochlorite, 15min; sterile water, 4-5 times, 5min / time. During the entire sterilization and cleaning process, the bottle should be shaken constantly to make the material fully c...

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Abstract

The invention relates to a tissue culture method of lycoris incarnata, and belongs to the technical field of plant tissue culture and rapid propagation. The tissue culture method of the lycoris incarnata comprises the following steps of 1, inoculating sterilized lycoris incarnata bulbs into a cluster-bud induction culture medium for induction culture to obtain first bulblets; 2, inoculating the first bulblets into a callus induction culture medium for inducing callus culture to obtain callus lumps; 3, inoculating the callus lumps to a callus differential culture medium for differentiation culture to obtain second bulblets; 4, inoculating the second bulblets to a bud strengthening and rooting culture medium for bud strengthening and rooting culture. The culture method is high in safety andstrong in differentiation capacity, and efficient breeding of the lycoris incarnata can be achieved.

Description

technical field [0001] The invention relates to the technical field of plant tissue culture rapid propagation, in particular to a method for tissue culture of Lycoris edulis. Background technique [0002] There are more than 20 kinds of Amaryllis plants, 15 of which are distributed in China. They have special ornamental characteristics of "flowers and leaves do not meet each other". They are also known as "Chinese tulips" and are very popular bulbous flowers all over the world. Lycoris is usually propagated by bulbing, bulb base cutting and tissue culture. The method of bulb division and bulb base cutting is simple and easy to implement, but the reproduction coefficient is not high and the speed is slow. It is difficult to meet the large demand for Lycoris bulbs in recent years. , Therefore, rapid propagation through tissue culture is an effective way to obtain a large number of Lycoris bulbs. [0003] At present, there have been many reports on the tissue culture of Lycori...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 张凤姣王忠孙海楠王涛庄维兵束晓春
Owner INST OF BOTANY JIANGSU PROVINCE & CHINESE ACADEMY OF SCI
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