Tissue culture method of lycoris incarnata
A culturing method and tissue culturing technology are applied in the field of tissue culture of lycorale, which can solve the problems of insufficient number of bulbs, lag in garden application, limited distribution area, etc., and achieves efficient propagation, improved callus induction rate, and protection and safety. Effect
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[0035] The high-efficiency tissue culture method of Lycoris provided by the invention adopts multi-hormone combination technology:
[0036] (1) Cultivate clustered buds from the bulbs of Lycoris
[0037] Take 2 healthy lycoris bulbs, clean them, remove the root tissue and the outer brown cortex with a knife, cut off the upper 2 / 3 of the bulb tissue, and keep 1 / 3 of the base of the bulb disc. Then carefully remove the 2-3 layers of scales outside the bulb, and then cut the bulb along the middle and divide it into 2 small pieces. Rinse the cleaned small pieces under running water for 30 minutes, soak in 0.1% Tween 20 aqueous solution for 30 minutes, rinse with clean water 2 to 3 times, transfer to the ultra-clean workbench, and perform the following sterilization process: 75% alcohol, 30s; 4 % sodium hypochlorite, 15min; sterile water, 4-5 times, 5min / time. During the entire sterilization and cleaning process, the bottle should be shaken constantly to make the material fully c...
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