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Coconut immature inflorescence tissue culture method

A tissue culture and immature technology, applied in the field of coconut immature inflorescence tissue culture, can solve the problems of poor test repeatability, failure to achieve success, long time, etc., to improve the callus induction rate, shorten the tissue culture period, and stabilize the method Effect

Active Publication Date: 2019-03-22
COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] Coconut is a highly heterozygous plant, and none of the culture media and cultivation methods published so far is widely applicable, and they are all limited to a certain type of genotype, or even in the same variety, some Plants can successfully induce callus, while some cannot
The Coconut Research Institute in Sri Lanka has a specialized tissue culture center, and they have been facing this problem for decades, which is difficult to overcome
[0006] 2. Poor test repeatability
The regenerated plants obtained by the Philippine Coconut Agency for the first time have already set fruit, but subsequent experiments have not been successful
[0008] 3. The tissue culture cycle is long
It will take longer to establish a mature technology system that can be used on a large scale
[0010] In addition, problems such as low reproductive coefficient and difficult reproduction need to be solved urgently.
[0011] In summary, there is currently no mature technical solution available for production

Method used

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  • Coconut immature inflorescence tissue culture method
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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0059] A kind of coconut immature inflorescence tissue culture method, comprises the following steps:

[0060] The immature inflorescence of Wenye No. 2 variety was taken as the explant; through the callus induction stage, callus propagation stage, somatic embryo induction stage, somatic embryo maturation stage, somatic embryo germination stage, regenerated plant strong root stage and grouping Cultivate at the stage of transplanting and cultivating seedlings;

[0061] 1.1 Callus induction stage:

[0062] The callus induction medium includes the following components: 250 μM 2,4-D, 5 μM BAP, 5 μM 2iP, 4 g / L phytogel, 3 g / L activated carbon and 30 g / L sucrose are added to the modified Y3 medium;

[0063] The pH value of the medium is 5.75, the culture condition is dark culture at 28°C, and the culture time is 1 month.

[0064] 1.2 Stage of callus propagation:

[0065] The callus propagation medium includes the following components: 250 μM 2,4-D, 5 μM BAP, 5 μM 2iP, 10 μМTDZ, 4...

Embodiment 2

[0081] A kind of coconut immature inflorescence tissue culture method, comprises the following steps:

[0082] The immature inflorescence of Wenye No. 2 variety was taken as the explant; through the callus induction stage, callus propagation stage, somatic embryo induction stage, somatic embryo maturation stage, somatic embryo germination stage, regenerated plant strong root stage and grouping Cultivate at the stage of transplanting and cultivating seedlings;

[0083] 1.1 Callus induction stage:

[0084] The callus induction medium includes the following components: 200 μM 2,4-D, 3 μM BAP, 3 μM 2iP, 2 g / L phytogel, 1 g / L activated carbon and 20 g / L sucrose are added to the modified Y3 medium;

[0085] The pH value of the medium is 5.75, the culture condition is dark culture at 27°C, and the culture time is 1 month.

[0086] 1.2 Stage of callus propagation:

[0087] The callus propagation medium includes the following components: 200 μM 2,4-D, 3 μM BAP, 3 μM 2iP, 5 μМTDZ, 2 ...

Embodiment 3

[0102] A kind of coconut immature inflorescence tissue culture method, comprises the following steps:

[0103] The immature inflorescence of Wenye No. 2 variety was taken as the explant; through the callus induction stage, callus propagation stage, somatic embryo induction stage, somatic embryo maturation stage, somatic embryo germination stage, regenerated plant strong root stage and grouping Cultivate at the stage of transplanting and cultivating seedlings;

[0104] 1.1 Callus induction stage

[0105] The callus induction medium includes the following components: 300 μM 2,4-D, 8 μM BAP, 8 μM 2iP, 8 g / L phytogel, 8 g / L activated carbon and 40 g / L sucrose are added to the modified Y3 medium;

[0106] The pH value of the medium is 5.80, the culture condition is dark culture at 29°C, and the culture time is 3 months.

[0107] 1.2 Callus propagation stage

[0108] The callus propagation medium includes the following components: 300 μM 2,4-D, 8 μM BAP, 8 μM 2iP, 20 μМTDZ, 8 g / L...

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Abstract

The invention discloses a coconut immature inflorescence tissue culture method. The method adopts immature inflorescence of coconuts as explants, and the formula of a culture medium is strictly controlled in each stage of tissue culture. The highest rate of callus induction can reach 97.2%, the highest rate of somatic embryo induction can reach 98.0%, the highest rate of regeneration of somatic embryos can reach 99.1%, and the highest transplantation survival rate can reach 97.5% after the tissue culture. The formula and the method of the culture medium provided by the invention are more broad-spectrum, and are suitable for various varieties such as Hainan local high varieties, Wenye No.2 and Wenye No.3. The culture method has good repeatability and short cycle, and is beneficial to factory and commercial application.

Description

technical field [0001] The invention relates to a tissue culture method for coconut immature inflorescences, belonging to the technical field of plant tissue culture. Background technique [0002] Tissue culture technology is a method of rapidly propagating clones using explants such as roots, stems, leaves, flowers, and fruits of plants, and has been successful in various plants. Tissue culture technique is the most effective vegetative method for rapid propagation of coconuts. As early as 1978, Fisher and Tsai attempted to use coconut embryos for tissue regeneration, but only obtained callus tissue and failed to regenerate plants. In 1994, Verdeil et al. successfully obtained regenerated plants from immature inflorescences of coconuts for the first time. Afterwards, despite the efforts of many research groups, the test-tube plantlets produced by this method are still unreliable and the reproducibility is not strong. Chan et al. (1998) obtained regenerated plants from coc...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00
CPCA01H4/001A01H4/008
Inventor 范海阔刘蕊
Owner COCONUT RES INST OF CHINESE ACAD OF TROPICAL AGRI SCI
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