Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Method for increasing content of benzyl isoquinoline alkaloids (BAIs) in Macleaya cordata (Willd.) R.Br. through over-expressed berberine bridging enzyme (BBE) and application of method

A technology of overexpression and overexpression, which is applied in the fields of application, horticultural methods, botanical equipment and methods, etc., can solve problems such as the depletion of medicinal plant resources, the degradation of varieties, and the reduction of active ingredients, so as to improve the callus induction rate and conversion rate effect

Inactive Publication Date: 2019-03-01
MICOLTA BIORESOURCE INC LTD
View PDF0 Cites 1 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

my country is rich in wild medicinal plant resources, but due to the destruction of the natural environment and the over-exploitation and abuse of medicinal plants in recent years, the medicinal plant resources in my country are on the verge of exhaustion
Wild resources can no longer meet people's needs, and there are problems such as the reduction of active ingredients and the degradation of varieties in artificial cultivation. Therefore, the use of biotechnology to regulate the secondary metabolism of medicinal plants can effectively improve the metabolic pathways of plants and increase the resistance of plants. Disease resistance, stress resistance, content of active ingredients, drug efficacy, yield and quality are of great significance for optimizing medicinal plant germplasm resources and sustainable development

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for increasing content of benzyl isoquinoline alkaloids (BAIs) in Macleaya cordata (Willd.) R.Br. through over-expressed berberine bridging enzyme (BBE) and application of method
  • Method for increasing content of benzyl isoquinoline alkaloids (BAIs) in Macleaya cordata (Willd.) R.Br. through over-expressed berberine bridging enzyme (BBE) and application of method
  • Method for increasing content of benzyl isoquinoline alkaloids (BAIs) in Macleaya cordata (Willd.) R.Br. through over-expressed berberine bridging enzyme (BBE) and application of method

Examples

Experimental program
Comparison scheme
Effect test

Embodiment Construction

[0054] In order to better illustrate the content of the invention, the present invention will be further verified by specific examples below.

[0055] 1. Cloning of McBBE and PsBBE genes

[0056] 1.1 Materials

[0057] 1.1.1 Reagents

[0058] Plant polysaccharide polyphenol total RNA extraction kit (TIANGEN RNAprep Pure Plant Kit) was purchased from Tiangen Biological Co., Ltd.; agarose gel DNA recovery kit (TaKaRa MiniBEST Agarose Gel DNA Extraction Kit), cDNA reverse transcription kit (PrimeScriptTM 1st Strand cDNA Synthesis Kit) was purchased from Bao Biological Engineering (Dalian) Co., Ltd. dsRNA HS Assay Kit and Taq enzyme were purchased from Yubao Bioengineering (Dalian) Co., Ltd. In- HD Cloning Kit, restriction enzymes KpnI-HF and XbaI-HF were purchased from NEB, and DNAmarker was purchased from Tiangen Biological Co., Ltd. Anhydrous ethanol was purchased from Sinopharm Chemical Reagent Co., Ltd. The gene fragment of PsBBE (berberine bridging enzyme in poppy) w...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

No PUM Login to View More

Abstract

The invention provides a method for increasing the content of benzyl isoquinoline alkaloids (BAIs) in Macleaya cordata (Willd.) R.Br. through over-expressed berberine bridging enzyme (BBE) and application of the method. The method comprises the steps: a complete encoding sequence of a key gene, namely the BBE participating in alkaloid metabolism in the Macleaya cordata (Willd.) R.Br. is amplifiedthrough a PCR technology, by combining with an In-Fusion homologous recombination method efficient vector construction technology, the BBE gene in the Macleaya cordata (Willd.) R.Br. is successfully integrated into a plant expression vector pCAMBIA2301D, and then the expression vector is guided into an explant of the Macleaya cordata (Willd.) R.Br. through an agrobacterium tumefaciens mediated method to increase the content of the BAIs in the Macleaya cordata (Willd.) R.Br.. The content of the certain BAIs, such as (S)-reticuline, (S)-scoulerine, (S)-cheilanthifolin and (S)-tetrahydrocolumbamine, in over-expressed Macleaya cordata (Willd.) R.Br. positive plants obtained through the method is remarkably increased by about three times, and the early foundation can be provided for deeply researching the BBE gene function and increasing the content of the BAIs in the Macleaya cordata (Willd.) R.Br..

Description

technical field [0001] The invention relates to a method and an application for overexpressing berberine bridging enzymes to increase the content of BAIs in Boluohui, belonging to the technical fields of genetic engineering and molecular biology. technical background [0002] Medicinal plants are plants that have the curative effect of preventing and curing diseases in Chinese traditional medicine and are used as the source of medicines. They have a very important position in Chinese medicine. The active ingredients of many medicinal plants are their secondary metabolites, which have attracted widespread attention due to their unique curative effect and low toxicity and side effects. my country is rich in wild medicinal plant resources, but due to the destruction of the natural environment and the over-exploitation and abuse of medicinal plants in recent years, the medicinal plant resources in my country are on the verge of exhaustion. Wild resources can no longer meet peop...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/53C12N15/82A01H4/00A01H5/00A01H6/64
CPCA01H4/00C12N9/0073C12N15/8243C12Y114/13037
Inventor 黄鹏曾建国
Owner MICOLTA BIORESOURCE INC LTD
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com