352 results about "Radicle" patented technology

The invention discloses a short-spike and root-grafting rapid propagation method for camellia azalea. The short-spike and root-grafting rapid propagation method comprises the following steps: selecting general camellia oleifera seeds, sterilizing the general camellia oleifera seeds with potassium permanganate solution and then carrying out stratification, sprouting and anvil culture; after radicles are 3-4 cm in length, grafting the radicles; selecting one-year branches with strongly-grown crown peripheries, full sprout eyes, no pest and disease damage and half lignification to lignification as scions with the length of 2-3 cm; soaking base parts with a plant growth regulator solution; after the scions are grafted, planting the grafted scions in a container bag filled with nutrient-rich substrate on a seed bed in a sunshelter for culturing; and covering, humidifying, shielding and carrying out standard management to form a high-quality grafted container seedling of the camellia azalea. According to the short-spike and root-grafting rapid propagation method, the grafting is realized at the smooth parts of radicles and the root systems of plants are developed, so that the survival rate and the growth amount of nursery stock can be effectively increased and the culture time of the stock is greatly shortened; and the scions are short, and thus the utilization rate and the propagation coefficient of the scions are improved. The short-spike and root-grafting rapid propagation method has the advantages of convenience for operation, high work efficiency and better economic benefit and social benefit; and according to the method, the scale propagation effect of the camellia azalea stock is ensured.

The invention discloses a new application of sodium hydrosulfide for promoting germination of wheat seeds under the stress of heavy metals, namely, the application is that the sodium hydrosulfide is taken as a sprout-promoting agent of germination in the soil polluted by heavy metals of Al<3+>, Cd<2+>, Cr<3+>, Cr<6+>, Pb<2+>, Hg<2> and the like. The invention can effectively improve the germinability of the wheat seeds under the stress of heavy metals, and induces the seeds to germinate fast and regularly. Research proves that the sodium hydrosulfide of the hydrogen sulfide donor is adopted to preprocess the wheat seeds, which can remarkably induce the activity expression of hydrolytic enzyme of amylase, protease, lipase and the like, in the seeds, enhances the oxygenolysis of storage matter, relieves the oxidative damage caused by the stress of the heavy metals in the seeds and maintains the lower membrane lipid overoxidation level in the seeds and the seedlings, thus being beneficial to the normal growth of embryo and radicle. The invention solves the key problem of restoring the production of the wheat in the areas polluted by heavy metals, has simple operation; the needed reagent is single and is easy to be obtained.

The invention provides a pharmaceutical composition for treating embolism and a preparation method thereof, the pharmaceutical composition comprises a hydroxyl-contained biocompatible polymer materialand a monomer containing unsaturated double bonds and anion groups, as well as a polymer generated by polymerization reaction of an optional vinyl monomer, wherein the polymerization is initiated through free radicles, and bleomycin or pingyangmycin is combined on the anion groups of the generated polymer. The preparation method combines the bleomycin or the pingyangmycin on a carrier of the polymer, thereby being capable of fully playing the dual effects of an anti-tumor antibiotic and a hardening agent owned by the bleomycin or the pingyangmycin during the embolism treatment. The anion partof the polymer can be properly combined with the bleomycin or the pingyangmycin which is rich in amino groups, thereby not only realizing the higher drug loading, but also leading the drug in an emboliaztion agent to be exchanged by cation in human body, and further realizing the slow release. In addition, the embolic carrier of the polymer has the advantages of simple prepration technology andlow cost, thereby being applicable to large-scale industrial production.

The invention discloses a method for efficiently and rapidly propagating Dendrobium candidum test-tube seedlings in a large scale. The method provided by the invention comprises the following steps of: disinfecting Dendrobium candidum capsules, rinsing and slicing off to obtain seeds; sowing seeds into a liquid culture medium and carrying out a shaking culture for 50-60 days; germinating seeds to form protocorms and further forming gemmules; putting the gemmules into a strong seedling culture medium to be cultured for 2-2.5 months to form plantlets with 2-3 leaves and 1-2 radicles; transferring the radicles into a strong seedling rooting culture medium and covering an opening sealing membrane on a bottle opening of a tissue bottle; transferring the tissue bottle to a plastic greenhouse seedling bed with an outer shade; providing sunlight or natural light and culturing for 2-3 months to obtain bigger seedlings; taking out the bigger seedlings from the bottle, washing, disinfecting and airing; then transplanting the bigger seedlings into cultivation matrix of a plastic greenhouse to be cultured for 24-30 months to obtain the product. The process provided by the invention can be directly used for industrially producing germchit in a large scale and has the advantages of less pollution, high seed germination rate, low variation rate, strong germchit, low production cost, high transplanting survival rate and the like.

The invention discloses the technical field of fruit tree planting and provides a planting method of cherry trees. According to planting treatment, fruit pits are taken out, and airing is performed to obtain seeds; 8-12-fold damp sand are mixed with the seeds, and the mixture is refrigerated in a refrigerator under the temperature of 0 DEG C; the mixture is placed into a 1:40-1:60 pig manure compost leaching solution and a gibberellin mixing solution with the concentration of 5% to be soaked, the soaking time is 5-10 min, the seeds are taken out after soaking is finished and sown in a seedling basket, a matrix with the ratio of turfy soil to river sand being 1:1 is adopted, and germination is accelerated under the room temperature; germination acceleration is stopped after radicles extrude seed coats and the length of the seeds is 1-3 times during seed germination, the germinated seeds are taken out and the seed coats are soaked into rooting powder. On the basis of seed sand storage freezing treatment, seed dormancy is broken through, the germination rate is effectively increased through soaking in the pig manure compost leaching solution and the gibberellin mixing solution, the seed germination rate is improved to 90% or above, the number and length of seed roots are effectively increased, the problem that a root system is shallow is avoided, and the rooting rate can be further increased through the rooting powder soaking solution.

The invention discloses an Ammopiptanthus introducing and cultivating method, which comprises the following steps of: sterilizing Ammopiptanthus seeds for 5-10 minutes by using 0.3-0.5% KMNO4, washing the Ammopiptanthus seeds for three times by using sterile water, immersing the Ammopiptanthus seeds for 10-15 minutes by using boiling water, immersing the Ammopiptanthus seeds for 12 hours by usingsterile water, and then putting out the Ammopiptanthus seeds; then, spraying distilled water on the immersed seeds at regular time, so that moisture required for seed imbibition is kept; and, fixedlyplanting the seeds on an aquiculture device after the seeds sprout to break through seed coats and radicle grows to 15-18 mm, transplanting seedlings in a farmland after 3-4 pairs of euphylla grow, and inevitably keeping soil moist after the seedlings are transplanted for one month. Compared with the existing Ammopiptanthus cultivating method, the introduction water cultivation method disclosed by the invention has the advantages of being saving in seedling space and resource, high in seedling survival rate, rapid in growth speed and convenient for transplanting; and seedling damage can be minimized.

The invention discloses an aseptic grafting method solving the difficulty of soybean transformed adventitious buds in rooting. Hypocotyls of soybean seedlings aseptically germinated are used as rootstocks. Genetically transformed soybean buds are scions to be grafted to the rootstocks. The buds grafted with the scions are provided with radicles below the hypocotyls with no need of reforming a root system, grafting is performed aseptically with no need of tedious operations of binding and fixing the scions and rootstocks, the buds which are grafted with the scions can heal with the rootstocks to grow into complete plants able to survive in transplanting and can also grow, develop, flower and fruit normally, and survival rate of grafted seedlings is 100%. The technical problem that the soybean transformed adventitious buds root difficulty is solved effectively.

The invention discloses a method for regenerating a plant by use of the callus of camellia japonica, belonging to the field of biotechnology. The method is characterized by comprising the following steps of: collecting the fruits of camellia japonica in early September, and disinfecting and cleaning; removing the inner and outer seed coats of seeds, and inoculating to a callus induction medium; transferring the material to a callus transition medium in the 25th -28th day; after the callus turns from light yellow into yellow with a little red, transferring the material into a callus differentiation adventitious bud medium; after the material is formed and bud sticks of 3-4cm grow, soaking the base part in IBA, and transferring to a paper bridge of an MV liquid medium to induce rooting; starting in the 20-22 days to grow transparent radicles with a little red; and then culturing for a certain period of time so that the radicels extend and are gradually lignified. The method for regenerating a plant by use of callus of camellia japonica, disclosed by the invention, provides a process with a relatively short period and a relatively high reproduction coefficient to large-scale asexual reproduction of camellia japonica, and lays a foundation for the molecular breeding of camellia japonica.

The invention discloses a method for mutagenizing barbadosnut seeds by ethylmethane sulfonate. The method disclosed by the invention comprises the following steps of: first, performing pre-bud forcing treatment at 30 DEG C on the barbadosnut seeds, selecting the seeds which are broken and exposed with unexpanded radicles after 3-7 days and soaking the seeds in a 0.9% EMS (Ethylmethane Sulfonate) liquor and soaking for 7 hours; forcing buds of the treated seeds at 28 DEG C and culturing for 3-4 days; and sowing mutagenized survival seeds in a soft peat soil substrate with radicles facing downward, and earthing by 1cm to breed. According to the method, the defects in the prior art are overcome, the mutagenizing concentration and time of EMS are reduced and the mutagenizing efficiency of the barbadosnut seeds by ethylmethane sulfonate is improved so as to facilitate selection by mutation of barbadosnut and promote industrialized development of the barbadosnut.

The invention discloses a method for promoting seed germination of the Kwangsi mayten herb. The optimal external factor combination of the seed germination of the Kwangsi mayten herb is that a constant temperature is 25 DEG C; illumination intensity is 3,000lx and illumination time is 12h/d; a culture substrate adopts peat soil; growth regulator treating fluid adopts gibberellin (GA3) with the concentration of 3mg/L. Under the conditions, not only is the survival rate the highest and reaches 88.0 percent, but also indexes of germinability, an average root length, germinating performance, the healthy and strong degree of radicles and the like can achieve better effects. The method lays a good foundation for nursery stock breeding of the Kwangsi mayten herb and has very active influence on regeneration and continuous utilization of resources of medicinal plants, which are in imminent danger and in shortage.

The invention provides a grafting method for hybrid citrus seed seedlings. The method comprises the following steps: (1) collecting seeds, namely starting to collect the seeds when fruits grow for 150 days, wherein the harvesting period of the seeds starts from flowering pollination; (2) breeding the seedlings, namely starting from radicle germination after the seeds are sown, breeding hypocotyledonary axes which vertically grow for 6-7 days until the length of the hypocotyledonary axis exceeds 2cm, cutting root tip growth points, keeping remaining radicles, mesocotyl thereon, cotyledon and germ parts, and breeding for 1-2 days for grafting; (3) performing grafting, namely cutting off the top ends of stock container seedlings which are one year old over the ground for 40cm, grafting hybrid seedlings at the cutting openings of the top ends of the stock container seedlings by using a cut grafting method, and sleeving a plastic film bag. According to the grafting method for the hybrid citrus seed seedlings, the seedling survival percent of the hybrid citrus seed seedlings is improved to be greater than or equal to 99 percent, the grafting survival rate is improved to be greater than or equal to 95.9 percent, and the growth of grafting buds is accelerated.

The invention relates to a new use of inducing wheat seeds to resist salt and bud through hydrogen sulfide donor sodium hydrosulfide, in particular to application of using hydrogen sulfide donor sodium hydrosulfide to improve the germination rate of wheat seeds under salt stress. The application can remarkably improve the germination situation of the wheat seeds under salt stress and promote the seeds to germinate fast and tidily. Research shows that the pretreatment of taking sodium hydrosulfide as hydrogen sulfide (H2S) can remarkably improve the activity of amylase, lipase, protease and other hydrolases in the wheat seeds, relieve the oxidative damage of the seeds because of the salt stress, maintain ionic relative balance in the internal microenvironment of the wheat seeds and promote the growth of germs and radicle. The application is single in reagent, easy to get the reagent and simple to operate, and provides a method with important reference value for solving the problem of sowing wheat on widely distributed alkaline land and salinized farmland.

The invention discloses a method for creating a new high oleic acid germplasm by treating rape germinating seeds with low-dose <60>Co-gamma rays, and belongs to a plant breeding method. The method comprises the following steps: firstly placing dried seeds of common rape in an illuminating incubator at 25 DEG C for germination treatment; carrying out low-dose radiation with the <60>Co-gamma rays after seed radicle germination, wherein, the radiation dose is 80Gray; preserving the radiation seeds in the illuminating incubator at 25 DEG C; and planting buds in a field after plumules grow to about 1cm. In the method, the oleic acid content of radiation progenies is directionally selected by a bulk method of breeding, and a plant N1379T with high oleic acid content up to 85% is obtained through positive breeding of continuous five generations.

The invention discloses a method for screening bio-control bacteria of cucumber fusarium wilt, and provides a method for identifying the bio-control nature of to-be-identified bacteria to pathogenic bacteria of the cucumber fusarium wilt including experimental treatment. The experiment treatment sequentially includes following steps of 1), accelerating sprouting of sterilized cucumber seeds to obtain the sprouting seeds; 2), subjecting the sprouting seeds to soaking in suspension liquid of the to-be-identified bacteria prior to cultivating the sprouting seeds till radicle length reaches by 1.5-2.0cm; 3), inoculating cucumber seedlings to suspension liquid of the pathogenic bateria of the cucumber fusarium wilt; 4), transferring the cucumber seedlings to MS solid media to cultivate, and identifying the bio-control nature of the to-be-identified bacteria to the patheogenic bacteria of the cucumber fusarium wilt by surveying disease indexes and/or observing disease phenotypes. The method is applicable to screening the bio-control bacteria of the cucumber fusarium wilt. In the method, the cucumber seedlings used as samples can truly reflect disease prevention effect of the bio-control bacteria, and can screen bacterial strains of various bio-control mechanisms.

The invention discloses aspergillus tubingensis with disease prevention and growth promoting functions as well as preparation and an application of aspergillus tubingensis metabolites. The bacterial strain No. of aspergillus tubingensis is QF05, and the registration No. of aspergillus tubingensis in CGMCC (China general microbiological culture collection center) is CGMCC No.13563. Aspergillus tubingensis QF05 has an inhibition function on botrytis cinerea, colletotrichum capsici, fusarium wilt of cucumbers, alternaria solani and pseudomonas syringae pv.lachrymans. Sterile fermentation filtrate of aspergillus tubingensis QF05 can remarkably increase the germination rate of pepper seeds and promote elongation of radicle. Aspergillus tubingensis QF05 metabolites containing bacteria effectively promote growth of true leaves of cucumbers and effectively increase the plant height and the yield of the cucumbers.

The invention discloses a seedling method for promoting growing of nursery stock lateral roots and belongs to a plant breeding method. The method includes that (1) collected and mixed seeds of water oaks and apocarya are disinfected and hidden in sand; (2) germinated seeds are taken out successively in next early spring; (3) after a part of radicles of the germinated seeds is cut, the germinated seeds are transplanted into a clean sand bed, a plastic tunnel is covered on the sand bed and used for preserving heat and moisture so as to promote germinating of seed embryos and growing of radicles; and (4) when seedlings of the seeds emerge, the seedlings are transferred from the sand bed, a part of newly grown roots are cut again, and the seedings are planted in a field according to certain strain row gaps. In a sand hidden period and a germination accelerating period of forest tree seeds, measures of cutting parts of radicles of the embryos twice are taken, a large amount of lateral roots and fibrous roots are seduced to grow at the root breaking positions of seedling main roots, and the number of the lateral roots and the fibrous roots are increased by 100-350%. Increasing of root system area promotes growth and development of the seedlings, proportion of portions of the seedlings above the ground and under the ground is adjusted, and survival rate of afforestation of seedling transplanting is improved. The method has wide application and popularizing prospects in nursery stock cultivating of deep-root tree varieties.

The invention relates to the technical field of rice cultivation, in particular to a special seed-dressing agent for rice. The special seed-dressing agent for rice is mainly prepared from 14-17% of enteromorpha prolifera polysaccharides, 10-15% of fulvic acid, 20-25% of oyster shell powder, 18-23% of 50% carbendazol, 24-28% of 25% azoxystrobin, 3-6% of sodium silicate, 0.003-0.005% of gibberellins and 0.004-0.006% of naphthylacetic acid. The special seed-dressing agent for rice is great in germination accelerating effect, capable of increasing the emergence rate of rice seeds and guaranteeing completeness, robustness and uniformity of seedlings and further capable of increasing chlorophyll and radicle amount of seedling leaves and effectively preventing soil-borne diseases such as pellicularia sasakii and rice blast of rice seeds.

The invention relates to nonconiferous cabbage nomadic small spore culture technique, and it belongs to biotechnology field. It is specially used in nonconiferous cabbage nomadic small spore culturing. It includes material getting and disinfect. The density of inoculate small spore is 1*105-6/ml, it is high temperature induced at 35 degrees centigrade heat induce 24~48 hours, dark development 15-20d is cultured under condition of 25 degrees centigrade. Then plant rebirth, subculture and transplantation are done. Chromosome is doubled quantitatively by adding 0.1 weight percent colchicines for treatment or using 0.1 weight percent colchicines dipping radicle in transplantation. The successful ratio of inducing nonconiferous cabbage gene type can reach 68.5 percent by using this invention, each flower bud introduce 4-10 in average, and even more than 92 at most.

The invention discloses an adventitious bud inducing and rooting medium for radicle of Carya cathayensis Sarg, which comprises four culture media for different induction periods: (1) a primary culture medium, (2) a radicle adventitious bud induction culture medium, (3) an adventitious bud enrichment culture medium, (4) an adventitious bud rooting medium. The four culture media all takes MS as a basic culture medium and are added with sucrose, agar A and other substances and have a pH value of 5.7. A tissue culture method for adventitious bud inducing and rooting of the radicle of the Carya cathayensis Sarg with the four culture media comprises the following five steps: 1, collecting and disinfecting explants; 2, performing primary culture; 3, inducing the adventitious bud of the radicle; 4, performing adventitious bud enrichment culture; and 5, performing rooting induction. The culture of the five steps lays a solid foundation for rapidly culturing saplings of the Carya cathayensis Sarg with good quality. When the medium is used for tissue culture, regenerated plantlet can maintain the excellent properties of parents; the propagation speed of the saplings is improved; the propagation number is expanded; seeds are effectively utilized; and the seedling period is shortened so as to contribute to meeting social requirements.

The method of the present invention includes the step of excising one or more portions selected from a radicle, a germ, and an embryonic axis of a plant tissue inoculated with Agrobacterium after cultivation in a coculture medium. The present invention provides a method of gene introduction that can transform a Triticum plant at high efficiency compared to conventionally known Agrobacterium methods, and provides a method of producing a transformed plant.

A 7-(4,4-dimethyl-3-aminomethyl pyrrolidine-1-yl) substituted neoquinoline carbonic acid derivative, which can be used as antibacterial agent or feed additive, and its preparing process are disclosed.

The invention belongs to the field of crop genetic engineering and relates to a sterile grafting method for solving the problem that barbadosnut converted adventitious budling can hardly take root. The sterile grafting method includes that the barbadosnut converted adventitious budling obtained by screening is taken as scions which are grafted to a hypocotyledonary axis serving as a stock and provided with part of radicle primordium, and the scions and the stock after grafted are good in degree of healing. By the application of the sterile grafting method, the problem that the barbadosnut converted adventitious budling can hardly take root can be well solved, and significant roles in obtaining more barbadosnut converted seedlings and improving quality of the same are played.

The invention provides a preparation method of a drought resisting seed soaking agent special for corn. The preparation method has the advantages that firstly, leach liquor is prepared by 'microwave-assisted secondary crushing plus synergistic extraction of double algae active ingredients by an enzymolysis approach plus double bacteria liquid co-fermentation', the leach liquor is rich in bioactive constituents such as mycose, natural organic matters, nitrogen phosphorus and potassium, and active bacteria, acts on seed surface layers, improves the drought stress resistance of corn seeds, directly supplies nutrition and effective biological active bacteria to the corn seeds, and greatly promotes seed germination; secondly, the special drought resisting seed soaking agent has a film forming characteristic, acts on the corn seed surface layers, forms natural biological activity protective films, and protects the seeds from a seeding phase to a sprouting phase, reduces evaporation of water contained in the corn seeds, promotes accelerated division of embryo cells, and enables radicles and germs to smoothly break through seed coats and germinate, and the protective films can be naturally degraded without residues; thirdly, a use method of the special drought resisting seed soaking agent is seed soaking or spraying, safety, environment friendliness, simpleness and high efficiency are realized, and the operation is facilitated.

The invention discloses a key gene LhHB9 for somatic embryo radicle elongation of liriodendron hybrids and application thereof. The DNAsequence of the key gene LhHB9 for somatic embryo radicle elongation of liriodendron hybrids is as shown in SEQ ID NO. 1. A cDNA sequence of the LhHB9 gene of liriodendron hybrids is cloned, an overexpression vector of the LhHB9 gene is constructed, liriodendron hybrids embryogenic callus is taken as a receptor material, and the genetic transformation is carried out by an agrobacterium-mediated method. After transgenic callus is obtained by G418 screening, it is detected and the transgene was preliminarily determined to be sucessful. Through the induction of the LhHB9 gene transgenic callus embryo, it is found that the transgenic somatic embryo radicle hasobvious elongation. The statistical data shows that the 35S:LhHB9 somatic embryo radicle is the most significant compared with the control CK, indicating that overexpression of the target gene can promote the elongation of the radicle and has good practicability.

The invention discloses Zea mays zinc iron-regulated transporter ZmZIP4 and ZmZIP6 genes and applications thereof. The Zea mays zinc iron-regulated transporter ZmZIP4 and ZmZIP6 genes are separated from Zea mays, and the cDNA sequences of the genes are represented by SEQ ID No.1 and SEQ ID No.3 respectively. Subcellular localization shows that a zinc iron-regulated transporter encoded by the two genes is localized in the plasma membrane and endoplasmic reticulum of a cell. Quantitative real-time RT-PCR expression analysis shows that the ZmZIP4 plays a role in the embryo growth process, and the ZmZIP6 is related to embryo maturation. Yeast complementation experiments show that each of the ZmZIP4 and the ZmZIP6 has a zinc and iron transportation function. The separated ZmZIP4 and ZmZIP6 genes have important application prospects in the regulation of zinc and iron absorption, transportation and storage of plants, and promotion of the embryo maturation, and radicle and germ growth.