36 results about "Radicle" patented technology

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The present application relates to the technical field of corn breeding, and particularly relates to a high-efficiency composite corn breeding device, which comprises a fixed frame and a lifting frame, a discharging box for placing corn is movably installed on the lifting frame, a discharging plate is arranged at the bottom of the discharging box, a plurality of discharging grooves are formed in the bottom of the discharging box, a discharging hopper which is communicated with the corresponding discharging groove is fixedly connected to the bottom of the discharging box, a inverted U-shaped lower hopper is fixedly connected to the bottom of the discharging hopper, and a discharging flap which is slidably connected with the corresponding discharging hopper is fixedly connected to the discharging plate; the discharging hopper and the discharging flap cooperate to form a V-shaped groove structure which is matched with the shape of the corn kernels, and the V-shaped groove structure is matched with the oscillation of the discharging box, so that the uniform downward directional sowing of the embryo root tips of the corn kernels is realized, the posture of the seeds into the soil is consistent, the seedlings are neat and uniform, and the risk of lodging is reduced; meanwhile, the pushing plate and the spreading plate are linked to quickly spread the corn kernels and improve the arrangement efficiency, and the excess corn kernels are collected and blocked to avoid mixed sowing, missed sowing and seed waste during discharging.

This invention discloses a seed germination radicle stress detection device and method based on the principle of polarized light. The seed germination radicle stress detection device includes a culture module, a polarized light imaging system, an adjustment and driving mechanism, and a data processing unit. The culture module includes a transparent culture chamber and a seed fixing support set inside the transparent culture chamber. The advantages of this invention are: utilizing the birefringence effect generated by light passing through the radicle sheath, continuous observation of the entire process from the initial germination to radicle breakthrough can be achieved without contacting or damaging the sample; by acquiring images through a multi-angle rotating analyzer and combining it with a phase delay algorithm, weak birefringence signals can be effectively extracted, realizing quantitative calculation and visualization of stress; the core components are general-purpose optical elements and imaging modules, with a simple structure, controllable cost, and easy to popularize in agricultural research and teaching.

The invention provides a bacillus amyloliquefaciens strain BaY6 and application thereof, and belongs to the technical field of microorganisms. The invention provides a bacillus amyloliquefaciens strain BaY6, and the preservation number of the bacillus amyloliquefaciens strain BaY6 is CGMCC (China General Microbiological Culture Collection Center) NO.28509. The bacillus amyloliquefaciens strain BaY6 has the advantages that the preservation number is CGMCC NO.28509; a radicle inoculation test and a potting test show that the bacillus amyloliquefaciens strain BaY6 has a relatively good inhibition effect on fusarium oxysporum, fusarium laminatum, fusarium solani and fusarium moniliforme, can effectively prevent and treat plant diseases caused by various fusarium, also has an effect of promoting plant growth, and in addition, the bacillus amyloliquefaciens strain BaY6 can be used for preventing and treating plant diseases caused by fusarium oxysporum, fusarium laminatum, fusarium solani and fusarium moniliforme. Field colonization experiments show that the bacillus amyloliquefaciens strain BaY6 can be colonized in soil for a long time, the effects of preventing and treating plant diseases and promoting plant growth can be achieved for a long time, and the research result provides a scientific basis for biological prevention and treatment of apple replanting diseases.

The application discloses an intensive cultivation method of rare medicinal materials with plant allelopathy, and relates to the technical field of Chinese medicinal material cultivation, and comprises a radicle safety domain, an outer edge protection domain and a cultivation unit with a directional selection and penetration interface between the two domains, wherein the radicle safety domain is filled with a germination and cultivation substrate, and the outer edge protection domain is filled with a protection substrate and is configured with an allelopathy source plant component; seeds are placed in the radicle safety domain and seedling raising management is performed, a net outward seepage component is formed by irrigation and water replenishment within a sensitive window from seedling emergence to slow seedling, so that inhibitory small molecules outward migrate at the directional selection and penetration interface and are intercepted and inactivated on the outer edge side, while water and inorganic ion supplies are maintained; the outer edge grass and bacteria inhibiting effect is continuously maintained in the early stage of planting and flux maintenance is implemented.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

The invention relates to the technical field of seed cultivation, in particular to agricultural planting seed cultivation equipment facilitating illuminance adjustment, which comprises a seedling tray, supports are arranged on two sides of the seedling tray, illumination lamps are arranged on the supports, a sliding plate is slidably connected to the supports, and a light-transmitting cover, a diffusion cover and a light gathering cover are arranged on the sliding plate. According to different illumination requirements of a seed germination stage, a seedling stage and a seedling stage, self-adaptive adjustment of illumination spectrum, intensity and distribution mode is realized, a blue light mode is automatically switched at the initial stage of germination, direct light is converted into uniform scattered light in cooperation with a diffusion cover made of a frosted material, and the uniform scattered light is uniformly scattered. The method has the advantages that tender radicles are effectively prevented from being burnt by strong light, a red-blue light mixing mode is switched in a seedling stage, the seedling strengthening index is increased by the synergistic effect of promoting stem cell division by red light and inhibiting excessive elongation by blue light, and meanwhile, the illumination intensity is enhanced by a high-light-transmittance acrylic light-transmitting cover, so that the growth requirement is met.

The invention belongs to the technical field of agricultural probiotic screening application, and particularly relates to a radiation-resistant bacterium and application thereof. The radiation-resistant bacterium is bacillus safensis, and the preservation number of the bacillus safensis is CCTCC (China Center for Type Culture Collection) NO: M 20251993. Radiation-resistant microorganisms are applied to the fields of saline-alkali soil improvement and stress agricultural production, the traditional cognition that the microorganisms are mainly limited to the fields of basic research or radiation protection for a long time is broken through, and important expansion of application scenes of the microorganisms is achieved. Under the adverse conditions of salt, alkali and the like, although the growth of the radiation-resistant strain R1 in the initial stage is possibly inhibited, the physiological activity can be rapidly adjusted and recovered, and the stable growth and metabolic function can be maintained. The germination rate of crop seeds treated by the strain is remarkably improved in salt stress and alkali stress environments, and robust growth of germs and radicles can be effectively promoted. In addition, the Bacillus safensis strain is a common safe microorganism in the environment, has excellent environmental compatibility, and has a safety basis and a practical prospect for large-scale popularization and application.

The invention relates to the technical field of agricultural intelligent cultivation, and discloses a peanut seed efficient germination cultivation method based on an improved culture medium. The method comprises the following steps: acquiring native state information of peanut seeds in a culture container, wherein the native state information comprises seed coat color, cotyledon plumpness and radicle initial form data; then comparing the information with a pre-stored characteristic spectrum to generate a seed state vector with a differentiation identifier; inputting the vector into a culture medium formula decision network, and outputting a targeted culture medium component adjustment scheme including mineral element ratio, organic substance concentration and gel dosage by the network; and finally, driving an automatic liquid preparation system to perform quantitative mixing and split charging according to the scheme to form a customized solid culture medium. According to the invention, a closed loop from seed state perception to full-automatic preparation of the personalized culture medium is realized, and the precision and efficiency of peanut seed germination cultivation are improved.

The invention relates to the technical field of green agriculture, in particular to a tersterione conjugated derivative as well as a preparation method and application thereof. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, wherein R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, and R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The terleastone conjugated derivative has a good inhibition effect on radicles and germs of euphorbia lunulata, the activity of the terleastone conjugated derivative is obviously higher than that of terleastone, and the terleastone conjugated derivative has a wide application prospect in the aspect of biological weeding.

This invention discloses a method and application for promoting root bending and lateral root formation in seed-sown persimmon (Diospyros kaki). This invention belongs to the technical field of persimmon seedling cultivation and bonsai material cultivation. The method includes low-temperature stratification treatment of persimmon seeds; disinfection and imbibition treatment of the stratified seeds followed by sowing in a gibberellin-containing GA3 medium; periodic artificial adjustment of the radicle growth direction when the radicle reaches 1 cm in length to induce taproot bending; removal of 1 cm of root tip when the taproot reaches 4 cm in length to promote lateral root and fibrous root germination; and subsequent hardening-off and transplanting. This invention, by regulating early geotropism of the radicle and taproot apical dominance, enables the taproot of persimmon seed-sown seedlings to form a natural bending shape and significantly promotes lateral root germination, resulting in good ornamental value, growth vitality, and promising application prospects.

The application discloses a synthetic bacterial flora capable of improving salt tolerance of alfalfa, which is composed of Pseudomonas strain EC5, Agrobacterium strain EC17, Streptomyces strain EC15, Paracoccus strain RH39, Pseudomonas strain RH6, Pseudomonas strain RH37, Sporosarcina strain RH34 and Bacillus strain RH32. The synthetic bacterial flora has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing siderophore, producing biofilm, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, plumule length and fresh weight of the group seedling embryo of alfalfa. Under the stress of 200 mmol / L NaCl, seed soaking with the synthetic bacterial flora can significantly improve the plant height, stem diameter, leaf area and fresh and dry weight of the above-ground part of alfalfa.

The invention relates to the field of corn breeding, in particular to a corn breeding germination device which comprises a breeding box body, a breeding unit arranged on the breeding box body, a spraying unit arranged on the breeding box body and a gas exchange unit arranged on the breeding unit. The phenomena of central waterlogging and edge drought are eliminated, the germination rate of corn seeds is increased, the output air pipe is matched to directly supply oxygen to the root system, the porosity of the substrate layer is maintained, the micro-vibration effect can be generated through intermittent airflow, substrate hardening is prevented, the root system activity is improved, and the cultivation effect is further improved; water sprayed on the substrate layer is absorbed and guided, so that the middle part and the bottom of the substrate layer are kept wet, the growth rules of radicle hydrotropism and germ geotropism are met, primary roots are promoted to be quickly pricked down and secondary roots are promoted to synchronously develop, and meanwhile, excessive water is guided out.

The invention discloses a method for improving alkali resistance of cotton. The method comprises the step of treating cotton seeds with a procyanidine solution, wherein the concentration of the procyanidine solution is 1-10 mM. The method disclosed by the invention is suitable for treating conventional upland cotton, such as Ji 228, Zhong H242, Xinautumn No.1, glyphosate resistance, Zhong 9807, Zhong 9835, Zhong S 9612, 2012 HB-5, HB09, Lu 8H7H5 and the like. As the growth stage of hypocotyl and radicle after seed germination is the key stage of stress-resistant growth of cotton plants, the germinated seeds are used for experiments, and a method for enhancing the alkali resistance of cotton in the salinization environment by adding procyanidine is established, so that the inhibition of hypocotyl and radicle during cotton germination can be relieved.

The invention belongs to the technical field of microorganisms, and particularly relates to a compost pseudomonas, a microbial agent and application of the compost pseudomonas, the compost pseudomonas is P430, the compost pseudomonas is preserved in the China Center for Type Culture Collection, the preservation date is November 18, 2024, the preservation number is CCTCC NO: M 20242583, the strain has high siderophore production activity, the siderophore activity unit is 44.2% in 72 h, and the siderophore production time is 24 h. Under salt stress, the soybean emergence rate can be remarkably increased, soybean seedling growth can be remarkably promoted, and the inhibition effect of salt stress on rape radicle and bud growth can be remarkably relieved.

The invention belongs to the technical field of plant tissue culture, and discloses a Paeonia fulva tissue culture and rapid propagation method for inducing cluster buds by using immature embryos, which comprises the following steps: collecting fresh seeds at a specific development stage, sterilizing, and stripping embryos aseptically; inoculating the embryos into a germination culture medium containing 6-BA, IAA and GA3 to start culture; after radicle tail ends of the germinating embryos are cut off, the embryos are directionally inoculated to a subculture medium according to the original polarity, and sterile seedlings are obtained; transferring the seedlings into an induction culture medium containing 6-BA, IAA and charcoal to induce cluster buds, and further proliferating; cutting robust tender shoots, removing cotyledons, and inoculating into a rooting culture medium containing IBA (indolebutyric acid), NAA (naphthylacetic acid) and charcoal to induce rooting; and finally, carrying out strong seedling culture and domestication transplanting to obtain a survived plant. According to the method, the deep dormancy obstacle of the seeds is broken through, efficient and stable regeneration from embryos to seedlings is achieved, the breeding cycle is short, the rooting and transplanting survival rate is high, and the method is suitable for large-scale protection and production of rare resources.

The invention discloses a cultivation method suitable for ledum palustre in a high and cold environment. The cultivation method comprises the following cultivation steps that seed sources are screened and collected; carrying out stress resistance treatment on the seeds; performing quality optimization configuration; three-stage stress-resistant domestication seedling raising; performing frozen soil isolation planting; dynamic environment monitoring; and overwintering management. According to the application, gibberellin physiological activation and program-controlled gradient freezing processes are adopted to induce cold-resistant protein (LEA protein) expression, so that the seed germination rate is increased from 38% to 86%, an antioxidant enzyme system in the seeds is activated by low-temperature stress, the SOD activity is increased by 2.1 times, the malondialdehyde content is reduced by 62%, and the cell membrane stability is remarkably enhanced; a temperature gradient decreasing and short-day stress seedling raising system is constructed, radicle germination and cotyledon expansion are promoted, the activity of a photosynthetic assimilation key enzyme Rubisco is stimulated to be improved by 40%, the chlorophyll a / b ratio is optimized to 3.2, the proline accumulation amount reaches 180% of that of wild seedlings, the soluble sugar content is improved by 90%, and a low-temperature resistance physiological basis is formed.

This invention discloses the application of the OsATS3 gene in regulating rice's tolerance to waterlogging stress. The invention constructs an OsATS3 gene editing and knockout vector, transforms rice callus tissue using Agrobacterium-mediated transformation, and screens for OsATS3 gene knockout lines. These knockout lines exhibit phenotypes such as well-developed seedling roots, faster aboveground seedling growth, faster first leaf emergence, and higher seedling survival rate after seed germination under waterlogging conditions. This is significant for addressing problems such as slow seedling growth, inhibited radicle development, and low seedling survival rate in direct-seeded rice, and has broad application prospects in plant breeding.

The invention discloses a seed treatment method for preventing aphelenchoides besseyi var. Oryzae infection, which comprises the following steps: step 1, selecting rice seeds and soaking with warm water, step 2, mixing a plant source active substance, a low-dose chemical nematicide, a slow-release carrier, a synergistic additive and deionized water to prepare a composite treatment solution, step 3, completely immersing the pretreated seeds into a composite treatment solution, and step 4, naturally airing the seeds for 2 hours after the soaking is finished, then drying the seeds with hot air, and finally packaging, sealing and storing the seeds, through the synergistic effect of the composite plant source active substance and the low-dose chemical nematicide and the slow release technology, efficient prevention and control of the aphelenchoides besseyi are achieved, direct damage of a high-concentration agent to radicle cells is avoided, the slow release carrier can reduce damage of oxidative stress to embryonic cells, and the effect of preventing and controlling the aphelenchoides besseyi is achieved. Nematodes in seeds can be effectively killed, secondary infection of soil nematodes on seedlings can be inhibited, seed germination and robust growth of the seedlings can be promoted, and the protection period is prolonged to reduce the pesticide application requirement.

The application discloses application of OspPLA V gene in regulation and control of rice submergence stress tolerance. The application constructs an OspPLA V gene editing knockout vector, transforms rice callus by using an agrobacterium-mediated method, and selects to obtain a knockout strain of the OspPLA V gene. The knockout strain of the OspPLA V gene shows the following phenotypes: under the submergence condition, the seed germination is followed by developed root system of the seedling, faster growth of the seedling shoot, faster emergence of the first leaf, and high seedling rate. The application has great significance for solving the problems of slow seedling growth, inhibited radicle development and low seedling rate in the rice direct seeding process, and has a wide application prospect in plant breeding.

This invention discloses a dry heat treatment method for tomato seeds to enhance seed vigor and extend storage life, relating to the field of tomato seed storage technology. The method includes: selecting mature, plump, and undamaged commercial tomato seeds with consistent maturity and maintaining appropriate moisture content; placing the pre-treated seeds in a single layer on a tray and placing it in a dry heat treatment device, performing dry heat treatment sequentially according to a preset program; comparing the germination indicators of dry heat-treated and untreated seeds to determine the effect of the dry heat treatment; and sealing and storing tomato seeds with qualified germination rates. This invention, through precisely controlled stepwise heating and high-temperature constant-temperature sterilization, effectively activates the seed's internal repair enzyme system while inhibiting metabolic processes that lead to seed deterioration, avoiding physical or physiological damage to the seeds caused by high-temperature treatment, and effectively preventing seed coat cracking, radicle burn, or excessive dehydration.

The present application relates to the technical field of plant tissue culture, and particularly discloses a method for establishing a grape seedling hypocotyl induced somatic embryo regeneration system, which comprises the following steps: taking secondary embryos obtained by using seedless grape embryos to rescue deformed seedling hypocotyls as explants, inducing the explants in KBN medium to obtain embryogenic calli, non-embryogenic calli and secondary proembryo groups, inoculating the secondary proembryo groups in X6 medium to proliferate and culture the secondary proembryo groups and somatic embryos, performing secondary induction on the somatic embryos before cotyledon stage to establish a somatic embryo maintaining and proliferating circulation system, inoculating the somatic embryos into X3 medium to differentiate hypocotyls and radicles, and transferring the somatic embryos to WPM medium to grow into seedlings. The present application successfully induces the regeneration system of the secondary proembryo groups obtained by using seedless grape embryos to rescue deformed seedling hypocotyls, and successfully establishes a secondary proembryo group maintaining and proliferating circulation system, which can provide new recipient materials for grape genetic transformation.

The invention belongs to the technical field of plant disease resistance identification, and particularly relates to a rapid identification method of muskmelon root rot resistance, which comprises the following steps: S1, expanding propagation of pathogenic bacteria and preparation of spore suspension; s2, culturing muskmelon inoculums: after accelerating germination of muskmelon seeds, culturing the muskmelon seeds until the radicle length is 1-2.5 cm; s3, inoculating and culturing; s4, investigating disease conditions in a grading manner and calculating a disease index DI; s5, evaluating disease resistance. According to the method, the identification stage is advanced from the seedling stage needing long-time growth to the radicle stage, culture is carried out under the optimal condition, the disease development time is shortened from 25-40 d to 7 d, the single-batch identification time can be shortened by 55-65%, and the processes of resistant germplasm resource screening, filial generation identification and breeding are effectively accelerated. Besides, a grading standard based on root direct affected symptoms is established, interference of non-specific symptoms of the overground part caused by environmental pressure can be avoided, the accuracy of resistance evaluation is improved, and a more reliable basis is provided for a breeder to select parents and offspring.

The invention relates to the technical field of plant genetic engineering and agricultural biology, and discloses a method for improving drought resistance of plants based on agrobacterium tumefaciens K599 and application of the method. The method comprises the following steps: cutting off radicles of germinating plant seeds, immersing wound sections into an infection bacterium solution containing a K599 strain and acetosyringone, transplanting, and culturing to obtain the transgenic hairy roots. Through optimization, the peanut transformation efficiency reaches 78.33%, and the method is suitable for various plants such as pelargonium odoratissimum and bryophyllum pinnatum. Taking the peanut AhCYP76b6 gene as an example, the leaf withering degree of an overexpressed plant subjected to drought treatment is obviously lower than that of a control group, and the drought resistance of the overexpressed plant is proved to be obviously enhanced. The method is easy and convenient to operate, high in transformation efficiency and high in cross-species applicability, and an effective tool is provided for plant drought-resistant gene function research and germplasm innovation.

This invention discloses a tissue culture method for Allium tuberosum, belonging to the field of tissue culture technology for Allium tuberosum. The method is as follows: (1) Aseptic treatment of explants: Select root tips of Allium tuberosum plants, disinfect them to obtain root tip explants; (2) Induction culture: Inoculate the root tip explants into the culture medium, culture them in the dark at 20-24℃ for 2-3 days, and then transfer them to the conditions of 20-24℃, light intensity 1500-2000 lx, and light time 10-12 h / day for 5-7 days to obtain root tip embryonic roots; (3) Proliferation culture: Inoculate the induced root tip embryonic roots into the proliferation culture medium, culture them at 20-24℃, light intensity 1500-2000 lx, and light time 10-12 h / day for 5-7 days to obtain root tip embryonic roots; (3) Cultivate for 15-20 days under the conditions of 500-2000 lx light intensity and 10-12 h / day; (4) Rooting culture: Transfer seedlings with a height of 2-3 cm to the rooting culture medium and cultivate for 8-10 days under the conditions of 20-24℃, 1500-2000 lx light intensity and 10-12 h / day. Then add the treatment agent and continue to cultivate for 7-10 days under the conditions of 24-26℃, 1800-2200 lx light intensity and 10-12 h / day. Then outdoor transplanting can be carried out, which solves the problem that mountain leek tissue culture seedlings are prone to vitrification and the tissue culture time is long.

The invention discloses a synthetic flora capable of improving salt tolerance of alfalfa. The synthetic flora consists of a pseudomonas strain EC5, an agrobacterium strain EC17, a streptomyces strain EC15, a paracoccus strain RH39, a pseudomonas strain RH6, a pseudomonas strain RH37, a sporosarcina strain RH34 and a bacillus strain RH32. The synthetic flora provided by the invention has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing iron carriers, producing biological membranes, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, germ length and colony seedling embryo fresh weight of alfalfa seeds. Under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved after the seeds are soaked by the synthetic flora.

The invention discloses a method for preparing tobacco metaphase chromosomes by utilizing young tobacco plants and application, and belongs to the technical field of preparation of tobacco chromosome specimens. The method comprises the following steps: germinating tobacco seeds at room temperature for 5-7 days, stripping seed shells when the seeds germinate and radicles grow to about 2 mm, sequentially performing N2O treatment on seedlings with radicles, fixing with a stationary liquid, performing enzymolysis in an enzymatic hydrolysate, lightly grinding plant tissues by using a sterile grinding rod until cells are dispersed, centrifuging, airing, adding glacial acetic acid, performing uniform vortex mixing, and performing freeze drying to obtain the tobacco seed suspension. And dripping a small amount of suspension on a clean glass slide to prepare the tobacco metaphase chromosome specimen. According to the method, materials are easy to obtain, the period is extremely short, operation is safe, the chromosome quality is high, and a reliable and efficient standardized scheme is provided for tobacco cytogenetics, molecular cytology and breeding research.

The invention discloses a preservation method for delaying lignification of picked mung bean sprouting vegetables in low-temperature storage. Fresh mung bean sprouting vegetables which are uniform in size, complete in radicle, free of aging signs, free of mechanical damage and free of microbial infection are selected and dried, and then the sprouting vegetables are subjected to red and blue LED light composite treatment. According to the method, the mung bean sprouting vegetables which are easy to lignify in low-temperature storage and difficult to maintain the edible value are used as research objects, and red and blue LED light composite treatment is adopted to prevent lignification. Researches show that the red-blue LED light composite treatment (the ratio of the red light to the blue light is 1: 1) can significantly inhibit the lignification of the fresh mung bean sprouting vegetables, and also can significantly inhibit weightlessness, and the shelf life of the mung bean sprouting vegetables treated by the method disclosed by the invention is prolonged to 8 days from the original 1-3 days.

The application belongs to the technical field of microbial application, and particularly relates to Stutzerimonas kunmingensis for improving salt tolerance of crops and application thereof. The Stutzerimonas kunmingensis is Stutzerimonas kunmingensis S246, the preservation number is CCTCC NO: M 20242582, and the Stutzerimonas kunmingensis S246 is preserved in China Center for Type Culture Collection on November 18, 2024. The strain can synthesize indole acetic acid and secrete iron carrier. The strain is used as an active ingredient to be applied to soybeans and oilseed rape, and the strain can significantly improve the emergence rate of dry crop soybeans in salt stress soil, increase the functional leaf area of soybeans, and promote the growth of soybean seedlings. The strain promotes the seed germination of oilseed rape in a salt stress environment, relieves the inhibition of salt stress on the growth of the radicle and bud of oilseed rape, and further enhances the adaptation of dry crop soybeans and oilseed rape to salt stress. Therefore, the Stutzerimonas kunmingensis S246 has a wide application prospect in promoting the seed germination, emergence and growth of crops such as soybeans and oilseed rape in saline-alkali farmland.