74 results about "Radicle" patented technology

The invention provides a germination form measurement method and system based on improved U-Net to realize automatic measurement, reduce labor cost and significantly improve efficiency, thereby optimizing tomato planting and management. According to the application, the seed germination form acquisition system is used for acquiring images of seed culture and seed germination processes, a data set is constructed, and the data set is used for training the improved U-Net model. According to the invention, the up-sampling of the model is replaced by the DBB module, and the CAA is introduced into the jump joint and the decoder. The MIOU index and the MPA index of the model can be respectively improved by 2.03% and 0.39% compared with those of the basic U-Net. According to the method, based on the segmentation result of the improved model, the radicle length can be extracted in combination with the canny algorithm. Compared with the ImageJ measurement result, the correlation coefficient of the model can reach 0.991, and the measurement efficiency is improved by 300 times. According to the method, the influence of drought, salinization and different molds on tomato radicle germination can be evaluated, and an efficient solution is provided for nondestructive monitoring and crop management in precision agriculture.

The present disclosure provides methods of gene transformation of sunflower based on Agrobacterium-mediated gene delivery to meristem cells of fresh imbibed / germinated sunflower seeds. One embodiment of the present disclosure is a method of directly transforming a sunflower from a mature seed with a heterologous polynucleotide, comprising: generating an explant from a germinated sunflower seed by removing cotyledons and primary leaves having intact meristem and tender shoot radicles; contacting the sunflower explant comprising the cotyledon with Agrobacterium comprising a heterologous polynucleotide in an infection medium supplemented with a plant hormone using vacuum and ultrasound treatment; co-culturing the explant from a germinating sunflower plant in a culture medium lacking the plant hormone in the presence of the Agrobacterium genus; culturing the mature seed explant in a regeneration medium comprising a selective agent; and culturing the seed with the regenerated tender shoots and with the secondary roots in a culture medium lacking the selective agent.

The invention discloses a growth-promoting rhizosphere bacterium streptomyces cerevisiae QS9-7 and application thereof, and belongs to the technical field of microorganisms. According to the invention, a strain of Streptomyces setonii QS9-7 is obtained through separation, and the preservation number of the Streptomyces setonii QS9-7 is CGMCC (China General Microbiological Culture Collection Center) No.32329. Experiments show that the strain QS9-7 has a relatively strong inhibition effect on various plant pathogens, and shows excellent biocontrol bacterium potential. The strain can effectively improve radicle length, germ length, lateral root number and germination rate of plant seeds and plant height and stem diameter of plant seedlings, obviously promotes plant growth, and has high application value in green prevention and control of plant diseases.

The invention relates to a streptomyces sp. Strain EC15 and an application of the streptomyces sp. Strain EC15. The streptomyces strain EC15 is preserved in the China General Microbiological Culture Collection Center (CGMCC), the preservation address is No.3, yard 1, Beichen West Road, Chaoyang District, Beijing, the preservation date is December 25, 2024, and the preservation number is CGMCC NO.33195. The streptomyces strain EC15 has the advantages that the streptomyces strain EC15 can be used for preparing the streptomyces strain EC15; the strain has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, producing a biological membrane, producing an iron carrier, producing IAA and producing ACC deaminase. The EC15 bacterial liquid is used for soaking seeds under the stress of 200 mmol / L and 250 mmol / L NaCl, so that the germination potential, the germination rate, the germination index, the radicle length, the germ length and the fresh weight of group seedlings of the alfalfa seeds can be obviously improved. After the EC15 strain is inoculated, under the stress of 200 mmol / L NaCl, the plant height, stem diameter, leaf area and overground part fresh and dry weight of alfalfa plants can be obviously improved.

The invention provides a composite flora for preventing and treating cotton verticillium wilt and a microbial agent and application thereof, and belongs to the technical field of agricultural microorganisms. The composite flora and the microbial agent provided by the invention comprise pseudoarthrobacter oxydans CRB-17 and bacillus velezensis CRB-46; the preservation numbers of the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are respectively CCTCC (China Center for Type Culture Collection) NO: M20252254 and CCTCC NO: M20252255, and the pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are both preserved in the China Center for Type Culture Collection on October 20, 2025. The pseudoarthrobacter oxydans CRB-17 and the bacillus velezensis CRB-46 are stably colonized in the rhizosphere of the cotton, have a strong direct antagonism effect on verticillium dahliae, can effectively and continuously prevent and treat the verticillium wilt of the cotton, effectively promote the germination of cotton seeds, the elongation of radicles and hypocotyls, promote the growth of the cotton and improve the defensive power of the cotton.

The invention provides a method for carrying out genetic transformation on safflower carthamus in a tissue-free culture mode, which comprises the following steps: cutting off radicles of newly germinated safflower carthamus seedlings, taking the radicles as explants, infecting the explants in a mode of smearing agrobacterium rhizogenes containing a target gene on wounds, burying the infected explants in soil for culturing, and carrying out tissue-free culture on the explants to obtain the safflower carthamus. The transgenic safflower hairy roots are obtained. The method fills the blank of a safflower non-tissue culture genetic transformation system, the whole transformation period is 20-30 days, and the method can be applied to rapid and efficient genetic transformation, gene editing and variety improvement of safflower.

The invention discloses a mountain land wind-resistant and drought-resistant persimmon tree planting method which comprises the following steps: transplanting mountain land persimmon tree grafted seedlings in spring of the first year, planting at least one rootstock seed seedling which just germinates and does not damage radicles around the grafted seedlings while planting the persimmon tree grafted seedlings or in advance, and cultivating the rootstock seed seedling into rootstock ground seedlings after one year; in spring of the next year, the rootstock ground seedlings are inarching to nearby grafted seedling trunks; before successful inarching, the planted grafted seedlings are supported manually in an auxiliary mode to resist wind. The technical problem that persimmon trees transplanted through an existing persimmon tree grafted seedling transplanting method are poor in wind resistance and drought resistance can be solved.

The present application relates to the technical field of corn breeding, and particularly relates to a high-efficiency composite corn breeding device, which comprises a fixed frame and a lifting frame, a discharging box for placing corn is movably installed on the lifting frame, a discharging plate is arranged at the bottom of the discharging box, a plurality of discharging grooves are formed in the bottom of the discharging box, a discharging hopper which is communicated with the corresponding discharging groove is fixedly connected to the bottom of the discharging box, a inverted U-shaped lower hopper is fixedly connected to the bottom of the discharging hopper, and a discharging flap which is slidably connected with the corresponding discharging hopper is fixedly connected to the discharging plate; the discharging hopper and the discharging flap cooperate to form a V-shaped groove structure which is matched with the shape of the corn kernels, and the V-shaped groove structure is matched with the oscillation of the discharging box, so that the uniform downward directional sowing of the embryo root tips of the corn kernels is realized, the posture of the seeds into the soil is consistent, the seedlings are neat and uniform, and the risk of lodging is reduced; meanwhile, the pushing plate and the spreading plate are linked to quickly spread the corn kernels and improve the arrangement efficiency, and the excess corn kernels are collected and blocked to avoid mixed sowing, missed sowing and seed waste during discharging.

This invention discloses a seed germination radicle stress detection device and method based on the principle of polarized light. The seed germination radicle stress detection device includes a culture module, a polarized light imaging system, an adjustment and driving mechanism, and a data processing unit. The culture module includes a transparent culture chamber and a seed fixing support set inside the transparent culture chamber. The advantages of this invention are: utilizing the birefringence effect generated by light passing through the radicle sheath, continuous observation of the entire process from the initial germination to radicle breakthrough can be achieved without contacting or damaging the sample; by acquiring images through a multi-angle rotating analyzer and combining it with a phase delay algorithm, weak birefringence signals can be effectively extracted, realizing quantitative calculation and visualization of stress; the core components are general-purpose optical elements and imaging modules, with a simple structure, controllable cost, and easy to popularize in agricultural research and teaching.

The invention provides a bacillus amyloliquefaciens strain BaY6 and application thereof, and belongs to the technical field of microorganisms. The invention provides a bacillus amyloliquefaciens strain BaY6, and the preservation number of the bacillus amyloliquefaciens strain BaY6 is CGMCC (China General Microbiological Culture Collection Center) NO.28509. The bacillus amyloliquefaciens strain BaY6 has the advantages that the preservation number is CGMCC NO.28509; a radicle inoculation test and a potting test show that the bacillus amyloliquefaciens strain BaY6 has a relatively good inhibition effect on fusarium oxysporum, fusarium laminatum, fusarium solani and fusarium moniliforme, can effectively prevent and treat plant diseases caused by various fusarium, also has an effect of promoting plant growth, and in addition, the bacillus amyloliquefaciens strain BaY6 can be used for preventing and treating plant diseases caused by fusarium oxysporum, fusarium laminatum, fusarium solani and fusarium moniliforme. Field colonization experiments show that the bacillus amyloliquefaciens strain BaY6 can be colonized in soil for a long time, the effects of preventing and treating plant diseases and promoting plant growth can be achieved for a long time, and the research result provides a scientific basis for biological prevention and treatment of apple replanting diseases.

The application discloses an intensive cultivation method of rare medicinal materials with plant allelopathy, and relates to the technical field of Chinese medicinal material cultivation, and comprises a radicle safety domain, an outer edge protection domain and a cultivation unit with a directional selection and penetration interface between the two domains, wherein the radicle safety domain is filled with a germination and cultivation substrate, and the outer edge protection domain is filled with a protection substrate and is configured with an allelopathy source plant component; seeds are placed in the radicle safety domain and seedling raising management is performed, a net outward seepage component is formed by irrigation and water replenishment within a sensitive window from seedling emergence to slow seedling, so that inhibitory small molecules outward migrate at the directional selection and penetration interface and are intercepted and inactivated on the outer edge side, while water and inorganic ion supplies are maintained; the outer edge grass and bacteria inhibiting effect is continuously maintained in the early stage of planting and flux maintenance is implemented.

The application discloses a kind of similar bundle-like arabino-galactoside protein coding gene GhFLA2 In the application of regulating cotton seed vigor, the application obtains several overexpression materials, RNA interference materials and gene knockout materials by transgenic technology GhFLA2 It is found that overexpression GhFLA2 Can promote radicle elongation during seed germination and cotyledon unfolding after germination, GhFLA2 After the expression amount of the application is reduced or knocked out, radicle elongation and cotyledon development are inhibited.The application provides a new gene target for cotton seed vigor improvement.

The invention relates to the technical field of seed cultivation, in particular to agricultural planting seed cultivation equipment facilitating illuminance adjustment, which comprises a seedling tray, supports are arranged on two sides of the seedling tray, illumination lamps are arranged on the supports, a sliding plate is slidably connected to the supports, and a light-transmitting cover, a diffusion cover and a light gathering cover are arranged on the sliding plate. According to different illumination requirements of a seed germination stage, a seedling stage and a seedling stage, self-adaptive adjustment of illumination spectrum, intensity and distribution mode is realized, a blue light mode is automatically switched at the initial stage of germination, direct light is converted into uniform scattered light in cooperation with a diffusion cover made of a frosted material, and the uniform scattered light is uniformly scattered. The method has the advantages that tender radicles are effectively prevented from being burnt by strong light, a red-blue light mixing mode is switched in a seedling stage, the seedling strengthening index is increased by the synergistic effect of promoting stem cell division by red light and inhibiting excessive elongation by blue light, and meanwhile, the illumination intensity is enhanced by a high-light-transmittance acrylic light-transmitting cover, so that the growth requirement is met.

The invention belongs to the technical field of desert plant cultivation, and discloses a sophora japonica seedling sterile culture method and application thereof, and the method comprises the following steps: collecting sophora japonica pods, shelling, and selecting sophora japonica seeds for later use; putting the culture medium into a nutrition bowl, putting the nutrition bowl into a light-transmitting culture bottle, and sealing the culture bottle by a breathable sealing film; performing steam sterilization, cooling and storing at room temperature; disinfecting the surfaces of the sophora japonica seeds, placing the disinfected sophora japonica seeds on a culture medium for accelerating germination, and finishing accelerating germination when radicles break through seed coats; under a sterile condition, sowing the germinated sophora japonica seeds in a culture medium, sealing a culture bottle, and transferring the sealed culture bottle to a culture room for culture to obtain sophora japonica seedlings. According to the method, a sterile culture system and a treatment method are provided, sophora argentea seedlings which are high in plant, developed in root system, large in leaf number and large in total fresh weight can be rapidly cultivated, and the sophora argentea seedlings can be used for interaction influence research of plants and microorganisms; the sterile sophora japonica seedlings can be obtained.

The invention relates to the technical field of crop performance detection, in particular to a rapid identification method and device for corn root nitrogen sensitivity, and the method comprises the following steps: providing a corn germination seedling with two side radicles consistent in length in a pair of side radicles; providing a high-nitrogen nutrient solution and a low-nitrogen nutrient solution; providing a set of device, wherein the device comprises two culture areas which are isolated from each other; adding the high-nitrogen nutrient solution into a first culture area, and adding the low-nitrogen nutrient solution into a second culture area; the root system of the single corn seedling is subjected to isolated culture, at least one side radicle of the single corn seedling is placed in the high-nitrogen nutrient solution of the first culture area, and meanwhile the other homologous side radicle of the single corn seedling is placed in the low-nitrogen nutrient solution of the second culture area; after a preset culture time, respectively measuring the lengths of lateral radicles in the high-nitrogen nutrient solution and the low-nitrogen nutrient solution; and evaluating the root nitrogen sensitivity of the single corn plant according to the difference between the lateral radicle length in the high-nitrogen nutrient solution and the lateral radicle length in the low-nitrogen nutrient solution.

The invention discloses a method for cultivating strong towel gourd seedlings through low-temperature treatment. The method comprises the following steps of shell breaking, wherein the edges of seeds are broken; seeds are soaked with warm water, and then the seeds are fished out, cleaned and spin-dried; accelerating germination, namely accelerating germination of the soaked seeds at 28-30 DEG C; sprouting: when the seeds begin to sprout, picking out the seeds which just sprout in batches; low-temperature treatment: placing the seeds with white buds under the low-temperature condition of 3-4 DEG C, and storing in a shading manner for 2 days; sowing: sowing the seeds subjected to low-temperature treatment in a seedbed, and covering with soil after sowing; and seedling cultivation: cultivating the seedlings into towel gourd seedlings. According to the method, the stage after germination and before sowing is selected, the method of treating the germinated seeds at low temperature is adopted, the storage time of the germinated seeds is prolonged, the radicle length is controlled, the germination potential is promoted to be relatively consistent, during one-time sowing, the seedling plant height and the hypocotyl length are reduced, the strong seedling index is increased, the seedling group growth vigor and quality are neat and consistent in appearance, and strong seedling formation is promoted.

The invention belongs to the technical field of agricultural probiotic screening application, and particularly relates to a radiation-resistant bacterium and application thereof. The radiation-resistant bacterium is bacillus safensis, and the preservation number of the bacillus safensis is CCTCC (China Center for Type Culture Collection) NO: M 20251993. Radiation-resistant microorganisms are applied to the fields of saline-alkali soil improvement and stress agricultural production, the traditional cognition that the microorganisms are mainly limited to the fields of basic research or radiation protection for a long time is broken through, and important expansion of application scenes of the microorganisms is achieved. Under the adverse conditions of salt, alkali and the like, although the growth of the radiation-resistant strain R1 in the initial stage is possibly inhibited, the physiological activity can be rapidly adjusted and recovered, and the stable growth and metabolic function can be maintained. The germination rate of crop seeds treated by the strain is remarkably improved in salt stress and alkali stress environments, and robust growth of germs and radicles can be effectively promoted. In addition, the Bacillus safensis strain is a common safe microorganism in the environment, has excellent environmental compatibility, and has a safety basis and a practical prospect for large-scale popularization and application.

The invention relates to the technical field of agricultural intelligent cultivation, and discloses a peanut seed efficient germination cultivation method based on an improved culture medium. The method comprises the following steps: acquiring native state information of peanut seeds in a culture container, wherein the native state information comprises seed coat color, cotyledon plumpness and radicle initial form data; then comparing the information with a pre-stored characteristic spectrum to generate a seed state vector with a differentiation identifier; inputting the vector into a culture medium formula decision network, and outputting a targeted culture medium component adjustment scheme including mineral element ratio, organic substance concentration and gel dosage by the network; and finally, driving an automatic liquid preparation system to perform quantitative mixing and split charging according to the scheme to form a customized solid culture medium. According to the invention, a closed loop from seed state perception to full-automatic preparation of the personalized culture medium is realized, and the precision and efficiency of peanut seed germination cultivation are improved.

The invention relates to the technical field of green agriculture, in particular to a tersterione conjugated derivative as well as a preparation method and application thereof. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, wherein R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The structural formula of the terreus ketone conjugated derivative is as shown in a formula 1, and R2 in the structural formula 1 comprises at least one of hydrogen, C1-3 alkyl, C2-3 alkenyl, C2-3 alkynyl, C1-3 alkoxy, C3-5 cycloalkyl, C3-5 cycloalkyloxy, halogen, nitryl, cyano, trifluoromethyl, 3-7-membered heterocyclic radical, C6-10 aryl and C6-10 heteroaryl. The terleastone conjugated derivative has a good inhibition effect on radicles and germs of euphorbia lunulata, the activity of the terleastone conjugated derivative is obviously higher than that of terleastone, and the terleastone conjugated derivative has a wide application prospect in the aspect of biological weeding.

This invention discloses a method and application for promoting root bending and lateral root formation in seed-sown persimmon (Diospyros kaki). This invention belongs to the technical field of persimmon seedling cultivation and bonsai material cultivation. The method includes low-temperature stratification treatment of persimmon seeds; disinfection and imbibition treatment of the stratified seeds followed by sowing in a gibberellin-containing GA3 medium; periodic artificial adjustment of the radicle growth direction when the radicle reaches 1 cm in length to induce taproot bending; removal of 1 cm of root tip when the taproot reaches 4 cm in length to promote lateral root and fibrous root germination; and subsequent hardening-off and transplanting. This invention, by regulating early geotropism of the radicle and taproot apical dominance, enables the taproot of persimmon seed-sown seedlings to form a natural bending shape and significantly promotes lateral root germination, resulting in good ornamental value, growth vitality, and promising application prospects.

The application discloses a synthetic bacterial flora capable of improving salt tolerance of alfalfa, which is composed of Pseudomonas strain EC5, Agrobacterium strain EC17, Streptomyces strain EC15, Paracoccus strain RH39, Pseudomonas strain RH6, Pseudomonas strain RH37, Sporosarcina strain RH34 and Bacillus strain RH32. The synthetic bacterial flora has the functions of dissolving organic phosphorus, dissolving inorganic phosphorus, dissolving potassium, producing siderophore, producing biofilm, producing IAA and producing ACC deaminase. Seed soaking and germination under the stress of 200 mmol / L and 250 mmol / L NaCl can obviously improve the germination potential, germination rate, germination index, radicle length, plumule length and fresh weight of the group seedling embryo of alfalfa. Under the stress of 200 mmol / L NaCl, seed soaking with the synthetic bacterial flora can significantly improve the plant height, stem diameter, leaf area and fresh and dry weight of the above-ground part of alfalfa.

The invention belongs to the technical field of gene engineering, and particularly relates to a method for rapidly inducing hairy roots of melon crops to detect gene editing efficiency, which comprises the following steps: connecting gRNA to be detected to a gene editing vector containing a red fluorescent protein gene expression cassette to obtain a recombinant gene editing vector; and transfecting agrobacterium rhizogenes by using the recombinant gene editing vector to obtain recombinant agrobacterium rhizogenes, cutting radicles from melon germinated seeds, transfecting the seeds by using the recombinant agrobacterium rhizogenes, sowing the seeds, and screening plants with red fluorescent hairy roots as transgenic plants to count the gene editing efficiency. According to the method, the detection period is greatly shortened to 15-20 days, the detection efficiency is improved, the operation is simple and convenient, the red fluorescent protein tdTomato is introduced, so that the identification of the positive root can be observed under an ultraviolet lamp irradiation condition or a naked eye condition, the operation visualization is realized, and the detection accuracy and convenience are greatly improved.

The invention relates to the field of corn breeding, in particular to a corn breeding germination device which comprises a breeding box body, a breeding unit arranged on the breeding box body, a spraying unit arranged on the breeding box body and a gas exchange unit arranged on the breeding unit. The phenomena of central waterlogging and edge drought are eliminated, the germination rate of corn seeds is increased, the output air pipe is matched to directly supply oxygen to the root system, the porosity of the substrate layer is maintained, the micro-vibration effect can be generated through intermittent airflow, substrate hardening is prevented, the root system activity is improved, and the cultivation effect is further improved; water sprayed on the substrate layer is absorbed and guided, so that the middle part and the bottom of the substrate layer are kept wet, the growth rules of radicle hydrotropism and germ geotropism are met, primary roots are promoted to be quickly pricked down and secondary roots are promoted to synchronously develop, and meanwhile, excessive water is guided out.

The invention discloses a camellia sasanqua radicle cuttage method, which relates to the technical field of plant cultivation, and comprises the following steps: selecting healthy and full camellia sasanqua flowers and fruits, and collecting camellia sasanqua radicles before the fruits are ripe; the camellia sasanqua radicles are disinfected and then soaked in a rooting accelerant; inserting the seedlings into a seedling culture container containing a mixed matrix of perlite, vermiculite and coco coir; the environment temperature interval is controlled to be 20-28 DEG C, the relative humidity interval is 75%-90%, and the illumination intensity interval is 3,000-6,000 lx; a nutrient solution is sprayed regularly, and the mixed matrix is kept moist; 45-60 days after cuttage, transplanting the camellia sasanqua radicles into nutrient soil for continuous cultivation. The technical breakthrough of camellia sasanqua cutting propagation is realized through key technologies such as radicle collection time optimization, hormone treatment, matrix proportioning, environment regulation and control and the like. Compared with a traditional cuttage method, the method has the remarkable advantages of being high in survival rate, rapid in rooting, developed in root system, high in adaptability and the like, is suitable for large-scale breeding, popularization and application of camellia sasanqua and has wide market prospects.

The invention relates to the technical field of directional sowing, in particular to a melon directional sowing method based on seed gravity center posture regulation, which comprises the following steps: placing melon seeds on a micro-vibration platform, collecting vibration trajectory data through a multi-axis sensor, and calculating the three-dimensional gravity center position of the seeds; determining the theoretical axis direction of radicle growth according to the three-dimensional gravity center position and the morphological characteristics of the pre-stored seeds; based on the theoretical axis direction, the clamping mechanism is controlled to adsorb the seeds at a grabbing angle parallel to the direction; under the state of maintaining the grabbing angle, the seeds are planted into the sowing holes, so that the theoretical axis direction of radicles is kept consistent with the gravity direction. According to the method, posture recognition is carried out by starting from a physical body and combining gravity center structure information, the problems that surface similar seeds are difficult to recognize and the misjudgment rate is high are effectively solved, the accuracy and adaptability of sowing posture judgment are remarkably improved, preconditions are provided for directional drip irrigation and root system induction in subsequent intelligent cultivation, and the method is suitable for popularization and application. And the mechanical integration is good.

The invention provides a high-efficiency juicy peach seed germination cultivation method based on an improved culture medium, which comprises the following steps: step 1, seed collection and pretreatment: seeds are subjected to low-temperature stratification treatment; step 2, preparation of an improved culture medium: based on the MS culture medium, adding the following components: 6-benzyladenine (6-BA), indolebutyric acid (IBA), gibberellin (GA), activated carbon, cane sugar and agar; step 3, seed germination and culture: inoculating the disinfected seeds to an improved culture medium under a sterile condition, keeping the culture medium moist, and promoting radicle breakthrough; step 4, seedling domestication and transplanting: when 2-3 true leaves grow on the seedlings, the seedlings are transferred to nutrient soil for domestication, illumination and humidity are adjusted step by step, and the seedlings are transplanted to a field after domestication. By improving the optimal proportion of the culture medium, the germination rate of the juicy peach seeds is remarkably increased. When the culture medium is improved, gibberellin (GA), 6-BA and other plant hormones are utilized, the dormancy period can be effectively shortened, the seed germination process is accelerated, and the seedling raising period is shortened.

The invention discloses a method for improving alkali resistance of cotton. The method comprises the step of treating cotton seeds with a procyanidine solution, wherein the concentration of the procyanidine solution is 1-10 mM. The method disclosed by the invention is suitable for treating conventional upland cotton, such as Ji 228, Zhong H242, Xinautumn No.1, glyphosate resistance, Zhong 9807, Zhong 9835, Zhong S 9612, 2012 HB-5, HB09, Lu 8H7H5 and the like. As the growth stage of hypocotyl and radicle after seed germination is the key stage of stress-resistant growth of cotton plants, the germinated seeds are used for experiments, and a method for enhancing the alkali resistance of cotton in the salinization environment by adding procyanidine is established, so that the inhibition of hypocotyl and radicle during cotton germination can be relieved.

The invention belongs to the technical field of microorganisms, and particularly relates to a compost pseudomonas, a microbial agent and application of the compost pseudomonas, the compost pseudomonas is P430, the compost pseudomonas is preserved in the China Center for Type Culture Collection, the preservation date is November 18, 2024, the preservation number is CCTCC NO: M 20242583, the strain has high siderophore production activity, the siderophore activity unit is 44.2% in 72 h, and the siderophore production time is 24 h. Under salt stress, the soybean emergence rate can be remarkably increased, soybean seedling growth can be remarkably promoted, and the inhibition effect of salt stress on rape radicle and bud growth can be remarkably relieved.

The invention belongs to the technical field of plant tissue culture, and discloses a Paeonia fulva tissue culture and rapid propagation method for inducing cluster buds by using immature embryos, which comprises the following steps: collecting fresh seeds at a specific development stage, sterilizing, and stripping embryos aseptically; inoculating the embryos into a germination culture medium containing 6-BA, IAA and GA3 to start culture; after radicle tail ends of the germinating embryos are cut off, the embryos are directionally inoculated to a subculture medium according to the original polarity, and sterile seedlings are obtained; transferring the seedlings into an induction culture medium containing 6-BA, IAA and charcoal to induce cluster buds, and further proliferating; cutting robust tender shoots, removing cotyledons, and inoculating into a rooting culture medium containing IBA (indolebutyric acid), NAA (naphthylacetic acid) and charcoal to induce rooting; and finally, carrying out strong seedling culture and domestication transplanting to obtain a survived plant. According to the method, the deep dormancy obstacle of the seeds is broken through, efficient and stable regeneration from embryos to seedlings is achieved, the breeding cycle is short, the rooting and transplanting survival rate is high, and the method is suitable for large-scale protection and production of rare resources.

The invention belongs to the technical field of plant molecular genetics, and particularly relates to a high-polysaccharide pumpkin identification primer pair and an identification method and application thereof. The invention discloses molecular markers Primer1 and Primer2 for identifying high-polysaccharide pumpkins, and provides two specific primer pairs with high polymorphism for identifying the high-polysaccharide pumpkins, and the nucleotide sequences of the two specific primer pairs are respectively shown as SEQ ID NO.3 to SEQ ID NO.6. The invention also provides a method for identifying the high-polysaccharide pumpkins. The method comprises the following steps: performing PCR amplification on DNA (deoxyribonucleic acid) of radicles, cotyledons, first new leaves or plant young leaves of pumpkin seeds through specific primers, performing agarose electrophoretic separation and EB dyeing, detecting polymorphism on an ultraviolet scenograph, and quickly identifying high-polysaccharide pumpkins according to differences of amplified products on marked polymorphic sites. The method has the advantages of accuracy, stability, low cost, simple operation and the like. Furthermore, the method can be used for pumpkin molecular marker-assisted breeding to improve the breeding efficiency and has a good application prospect.