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Grafting method for hybrid citrus seed seedlings

A technology of seedlings and seeds, which is applied in the field of grafting of citrus seedlings, can solve the problems affecting the length of hybrid citrus childhood and the long cycle of hybrid breeding, so as to improve the survival rate of grafting, have economic and technical feasibility, and increase the yield. The effect of seedling rate

Active Publication Date: 2015-06-03
CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

These stages all affect the length of the childhood period of hybrid citrus. "Childhood" refers to the number of years from seed sowing to flowering. The hybrid breeding cycle of existing methods is relatively long

Method used

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  • Grafting method for hybrid citrus seed seedlings
  • Grafting method for hybrid citrus seed seedlings
  • Grafting method for hybrid citrus seed seedlings

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] In this example, the hybrid of Yamashita red Wenzhou satsuma xW Murcott orange was used as the test material. Seeds were collected on September 15, 2012, and the seed development period was 150 days. The seedlings of the erect root system were cultivated by germination, and were grafted in two ways.

[0030] The grafting method of the present invention, (1) selects for use through about one year of pre-cultivation, the good annual Ziyang fragrant orange rootstock container seedling of uprightness; Grading by the size of rootstock seedling and nursery stock height, height difference 10cm classification is put neatly; Minimum control height is not The height of the seedlings is less than 50 cm, and the thickness of the seedlings at the root and neck is not less than 0.4 cm; on the day of grafting, keep the above-ground part of Ziyang orange container seedlings of similar size to more than 40 cm, and the rootstock seedlings of the same level are cut from the same height, an...

Embodiment 2

[0040] A method for grafting citrus hybrid seedlings, comprising the following steps:

[0041] (1) Seed collection: Seed collection begins 150 days after fruit development; the seed collection period is calculated from the time of flowering and pollination.

[0042] (2) Seedling cultivation: Peel off the outer and inner layers of seed coats of hybrid citrus seeds that are not yet fully mature, and cultivate them without soil at a constant temperature of 28°C until the seedlings germinate. From the germination of the radicle, cultivate the hypocotyls that grow upright After cultivating for 7 days, until the hypocotyl length reaches more than 2cm, the root tip growth point is cut off, and the rest of the hypocotyl is kept (the remaining radicle and the mesocotyl, cotyledon and embryo part thereon are taken as a scion whole), Cultured for another 1 day for grafting;

[0043] (3) Grafting: ①Select the annual Ziyang orange rootstock container seedlings with good uprightness after ...

Embodiment 3

[0054] After adopting the grafting method of embodiment 2, some hybrid seedlings have early flowering phenomenon ( image 3 ). The early flowering phenomenon refers to the phenomenon that after the citrus seeds are sown, although they are not physiologically mature and cannot pass through childhood normally, they can bloom directly from the terminal buds. The early flowering phenomenon generally refers to the direct flowering of the terminal buds of the seedlings within one year, and also includes the direct flowering of some test-tube seedlings in the test tube. However, plants with early flowering phenomenon often do not bear fruit directly. The present invention is also beneficial to early flowering and fruiting of individual plants that bloom early. See image 3 .

[0055] The fastest speed in the early flowering plant population is the seeds harvested on September 7, 2013. On September 18 of that year, the seedlings were grafted with the present invention, bloomed on ...

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Abstract

The invention provides a grafting method for hybrid citrus seed seedlings. The method comprises the following steps: (1) collecting seeds, namely starting to collect the seeds when fruits grow for 150 days, wherein the harvesting period of the seeds starts from flowering pollination; (2) breeding the seedlings, namely starting from radicle germination after the seeds are sown, breeding hypocotyledonary axes which vertically grow for 6-7 days until the length of the hypocotyledonary axis exceeds 2cm, cutting root tip growth points, keeping remaining radicles, mesocotyl thereon, cotyledon and germ parts, and breeding for 1-2 days for grafting; (3) performing grafting, namely cutting off the top ends of stock container seedlings which are one year old over the ground for 40cm, grafting hybrid seedlings at the cutting openings of the top ends of the stock container seedlings by using a cut grafting method, and sleeving a plastic film bag. According to the grafting method for the hybrid citrus seed seedlings, the seedling survival percent of the hybrid citrus seed seedlings is improved to be greater than or equal to 99 percent, the grafting survival rate is improved to be greater than or equal to 95.9 percent, and the growth of grafting buds is accelerated.

Description

technical field [0001] The invention relates to the hybrid breeding of citrus, in particular to a method for grafting citrus seed seedlings. Background technique [0002] "Self-crossing is harmful, hybridization is beneficial" is a common phenomenon in nature. Purposeful sexual hybridization is an important means of cultivating new plant varieties. [0003] About the method for cultivating hybrid citrus varieties in the prior art, it all will go through the stages of "combining hybridization combination pollination, hybrid fruit management, picking hybrid seeds, and seedling cultivation (seedling refers to the sapling formed by seed germination, thus distinguishing Grafted seedlings), grafted seedling stage and post-management” and other stages. The traditional method is to gather fully ripe fruit, or pick the fruit for storage when the hybrid fruit is eight mature, and take out the seeds to cultivate seedlings in the spring of the following year. When the seedlings grow ...

Claims

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Application Information

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IPC IPC(8): A01G1/06
CPCA01G2/30
Inventor 曹立彭良志淳长品江才沦凌丽俐谌智慧
Owner CITRUS RES INST OF CHINESE ACAD OF AGRI SCI
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