724 results about "Graft survival" patented technology

Methods are provided for evaluating a subject for graft survival, e.g., in terms of predicting graft survival, identifying the presence of a deleterious graft condition, such as CAN and DT, identifying the severity and class of acute rejection, etc, in a subject are provided. In practicing the subject methods, the expression of at least one gene in a sample from the subject, e.g., a blood or biopsy sample, is assayed, e.g., at the nucleic acid and / or protein level, to evaluate the subject. Also provided are compositions, systems and kits that find use in practicing the subject methods. The methods and compositions find use in a variety of applications.

The invention discloses a container seedling raising method for camellia oleifera bud-stock grafting. The container seedling raising method mainly comprises the six steps of cultivating camellia oleifera bud stocks, selecting and preparing nursery soil, preparing nutritional bags, preparing cions, grafting and transplanting and managing grafted container seedlings. According to the grafting method disclosed by the invention, the stocks are not injured, when packaging is performed, grafting ports can be completely closed, the moisture retention is good, the grafting survival rate can achieve 97%, and big seedlings, sound seedlings and improved seedlings can be cultivated; and furthermore, the container seedling raising method is simple and fast to operate, the complex camellia oleifera improved bud-stock grafting becomes simple and fast, and the container seedling raising method is further easy to learn, easy to understand and easy to operate.

The invention relates to a seedling growing method of apocarya and belongs to the technical field of fruit trees and forest cultivation. The method includes the steps of sprouting breeding, grafting and post-grafting management. By means of the method, a large amount of scions are obtained each year and are grafted on the stock and the seedling with strong affinity in due time, so that stock grafting survival rate of the apocarya is improved. Grafted seedling preserving rate after large-amount grafting is over 90%. Further, the method achieves tree body stuntedness, shortens the juvenile phase and enables tree bodies to fruit earlier in the fourth year after grafting. The fruiting time is 6-8 years earlier than before. Simultaneously, the method provides a growing method for apocarya stock grafting seedlings and with strong operability for batch and variety seedling growing of the apocarya and greatly increases economical value of the apocarya.

The invention relates to a healing agent for jujube grafting and an application method thereof, and belongs to a plant regulator. The healing agent comprises an agent A, an agent B and an agent C, wherein the agent A comprises naphthylacetic acid, heteroauxin and gibberellin; the agent B is carbendazim; the agent C is a nutrient. The application method comprises the following steps: dissolving the agent A into 60-85% of alcohol, mixing with the agent B and the agent C to prepare a healing solvent solution; soaking a jujube scion into the healing agent solution for 5-20 minutes and then using. By adopting the healing agent, a plant graft union growth regulator, nutrient, sterilization and moisture are achieved at the same time, and the healing agent has the functions of promoting callus formation, disinfecting, sterilizing, supplying nutrients, preventing moisture loss and the like. Not only is the jujube grafting survival rate improved by adopting a scion soaking method, but also the grafting speed is obviously improved, the labor intensity is reduced, and the grafting cost is saved.

The invention discloses a grafting method of camellia bud stocks, comprising the steps of: taking camellia bud seedlings which are not lignified or half-lignified as parental stocks, wherein, the camellia bud seedlings have 3-5cm height and more than 0.2cm diameter; taking the annual shoot camellia mother trees which are lignified or half-lignified as cions; grafting the stocks and the cions; packing grafting openings with preservative films when grafting; and sticking the preservative films with fire head irons. The invention adopts a preservative film-ironing technology, does not hurt the stocks, can completely seal the grafting openings when packaging, has good moisture retentiveness, has the hardly oxidized grafting openings which are fast healed, and has grafting survival rate which is high to 94.5%. Furthermore, the invention has simple and fast operation, can complete the grafting process by sticking the preservative films by means of light ironing with pastille fire head after being packaged, simplifies the complex grafting process of the camellia bud stocks, and is easy to learn, understand and operate. Therefore, farmers can fast skillfully master applied practical technique.

The invention provides a litchi grafting method, which comprises the following steps of scion and stock selection, grafting preparation, grafting and post-grafting management. Seedling litchi seedlings have arbor wood roots, the root systems are developed, and the grafting survival rate is ensured through selecting the seedling litchi seedlings as stocks; the seedling litchi seedlings and high-quality litchi seedlings belong to one family, the affinity between scions and stocks is high, the grafting success rate is greatly improved, and the growth speed of the grafted seedlings after the grafting can also be accelerated; the litchi seedlings have higher drought resistance capability, and the grafting survival rate can be ensured under the drought conditions, so the grafted litchi seedlings can bloom and bear fruit in three years. The litchi grafting method has the advantages that simplicity and convenience are realized, the survival rate is high, the coverage rate of high-quality litchi varieties can be improved, and the advanced blooming and fruiting of litchi can be realized.

A method is disclosed for preparing a soft tissue site, and augmenting the soft tissue site, such as the breast(s) of a subject through use of devices that exert a distractive force on the breast(s) and grafting of autologous fat tissue such as domes with sealing rims for surrounding each of the breasts and a regulated pump. The method for preparing the soft tissue site, and enhancing fat graft results, entails application of the distracting force to the targeted soft tissue site for several weeks prior to the graft procedure. A related aspect of the invention includes following the preparation steps by transfer of fat from other areas of the subject to the subject's breast(s), and then reapplication of the distractive force to the breast(s) that received the autologous fat graft. Alternatively, fat from genetically related sources may be used, and the fat may be further processed prior to injection. Substantial breast augmentation, high rates of graft survival and negligible graft necrosis (data demonstrating 80% survival and only 20% necrosis is presented) or calcification result from the practice of these methods.

The invention relates to a cion treating and preserving method. Annual shoots or secondary shoots of over 0.3cm thickness that grow well and are free from damage by disease and insect are selected and cut off 0.5cm above sprout; cut-off shoots are sterilized and socked in 50ppm of solution of growth regulating agent for green plants for 2h, taken out and dried by airing, then mixed with commercial wax of 54 degrees, refined lard and colophony, ratio of which is 1:0.5:0.2, and heated to 100-200 DEG C; then the cion acquired is dipped into wax, wrapped by white thin film and put into a refrigerator for 1-6 months at the temperature of 0 DEG C to 4 DEG C; the cion is taken out, heated and transplanted as by a normal method in due time for grafting. Cion preservation: wax-sealed cion is stored at the temperature of 0 DEG C to 4 DEG C for further use; grafting is carried out when tree sap flows. The invention can prolong the preservation time of the cion and improve grafting survival rate; branches qualified for grating are treated in combination with winter pruning, and are taken out and grafted in due time.

The invention relates to 'a bark grafting method of apocarya' and belongs to the field of fruit trees and forest tree breeding. The method includes cutting a stock or a lignification branch of the apocarya in vigorous growth to the xylem, conducing inclining cutting on the portion above a cut to form a 45-degree cut, cutting downwards on an edge bark layer of the cut through a stem grafting knife for 3cm with cutting length as 3cm and cutting width determined by scion thickness and conducting scion branch processing and grafting. By means of the method, summer grafting survival rate of scions dormant in winter is greatly improved, the problem of scion grafting survival of ligneous plants like the apocarya which is introduced from the southern hemisphere in summer is effectively removed, and simultaneously grafting period of the apocarya can be prolonged.

The invention belongs to the field of fruit tree and forest tree breeding and specifically relates to a high-scion and head-changing grafting method of carya illinoensis. The high-scion and head-changing grafting method of carya illinoensis comprises the following steps of: before sprouting of carya illinoensis in spring, keeping the trunk by about 1 m away from the ground surface and sawing off the part thereabove to form a stumping cut, and then downwards cutting open at the (symmetrical) edges of the two sides of the stumping cut by using a grafting knife, thereby forming two cuts which are 3cm long and have width determined according to the thickness of the scion, and then grafting; next, bounding with a plastic film, binding the graft with a kraft bag, wherein the diameter of the kraft bag is determined by the thickness of the stock, sawdust is filled in the kraft bag to be higher than the scion, and the humidity of the sawdust is 70%; and then tightening the upper opening of the kraft bag; and finally, covering the kraft bag with a plastic bag; one month later, removing the plastic bag is removed and tearing off the kraft bag. With the method, the high-scion grafting survival rate of carya illinoensis is higher than 98%; therefore, the high-scion grafting survival rate of carya illinoensis is effectively realized.

The invention discloses a method for the high square bud grafting of Juglans sigllata Dode. The method comprises the following steps: selection of the appropriate grafting period, collection and storage of scions as well as the grafting, and management after grafting. According to the invention, a summer high square bud grafting method is adopted to reduce the erosion to a grafting bud caused by the bleeding sap of the Juglans sigllata Dode, so that the high grafting optimization for Juglans sigllata Dode low yielding trees is successfully realized, the grafting survival rate reaches 78 to 92 percent, in addition, wounds can be healed up quickly, the operation is simple, and the grafting efficiency is high and a tree crown can recover quickly, so that the breed improvement problem of Juglans sigllata Dode in Guizhou mountainous area is solved. The method for the high square bud grafting of the Juglans sigllata Dode is simple and low in cost, and has a good using effect and a broad prospect for industrialization application.

The invention discloses a method for propagating apocarya seedlings. The method comprises the steps of selecting an annual apocarya seedling branch, shearing the branch to be the length being 10 cm, and soaking the lower end of the sheared branch into an 800 mg / L NAA solution for 4 h; placing a matrix into a paper bag, inserting a cutting into the matrix, enabling the paper bag obtained by cuttage to tightly abut against a mesh disc with a hole, and placing the paper bag on a seedbed; in the cutting rooting process, keeping the soil humidity to be about 70%, and keeping the temperature of the lower end of the cutting to range from 25 DEG C to 30 DEG C; conducting grafting 40 days after cuttage, adopting the bark peg grafting method, transferring the kraft paper bag, obtained after grafting, with the cutting into a large pot, filling the periphery of the pot with the matrix, pulling out 4 / 5 of the kraft paper bag, observing the grafting survival rate 15 days after grafting, completely removing the paper bag, and then conducting high-density nutrient and water management. According to the method, the apocarya nursery stock can grow more than 80 cm in the current year, and the survival rate is more than 90%.

The invention discloses a macadamia nut green branch grafting method. The method comprises enabling half-lignified green branches with 1 / 2 of leaf blades to serve as cion, enabling normally-growing nutrition bag seedlings without limiting seedling ages to serve as rootstock, adopting a cut-grafting method to perform grafting, and after grafting, planting the rootstock in a plastic film arched shed under a sunshelter to perform cultivation and management. The half-lignified green branches serve as the cion to be grafted to the nutrition bag rootstock seedlings. Due to the fact that lignification of the cion is low, tissue cells are active, calluses are formed quickly, the reserved leaf blades can make nutrient substance through photosynthesis so that the nutrient substance is supplied to grafting openings for healing, the macadamia nut green branch grafting method improves grafting survival rate by 15-20% in comparison with a hard branch grafting method, and the grafting survival rate is over 95%. The grafting survival rate of macadamia nut is greatly improved, a nursery stage is shortened, seedling cultivating cost is reduced, and the grafting survival rate is not limited to time and rootstock roughness. Besides, in the green branch grafting process, regardless of the seedling ages, as long as the half-lignified green branches are available, the green branches can be grafted all the year round, and the macadamia nut green branch grafting method is suitable for multiple effects of massive industrial seedling culture and provides technological support for macadamia nut seedling culture development.

The invention relates to a mature mango breed rapid updating dwarfing culture method. Cutting trunk time has no season restriction, grafting is immediately carried out after cutting trunk, cut grafting method is adopted, plastic film is adopted to cover grafting sprout, and tearing film is used for banding, and management of plant diseases and insect pests and intertillage fertilization are cared after grafting. The invention has the beneficial effects that: when the method is used for rapidly replacing breed, dwarfing and especially improvement of breed of mature mango garden and inferior mango garden, on one hand grafting survival rate is improved, breed improvement period is shortened, on the other hand, the improved fruit garden goes into operation early, economic benefit is increased, the aim of dwarfing the crown of mango tree can be realized by adopting the method, fruit picking and plant diseases and insect pests operation are easier, labour and time are saved, and management cost is reduced.

The invention provides a grafting and seedling-cultivating method for a Theaceae plant. A grafting and seedling-cultivating manner of the grafting and seedling-cultivating method is that seeds of the Theaceae plant are refrigerated; pre-germination is conducted in due time according to the shoot growing condition of a scion supply plant at every time, and the pre-germinated seeds are cultivated into root stocks in a seedling cultivating container; and a root stock 1.2 to 2.0mm in diameter is selected, and the upper end of the root stock is cut off at a distance of 3 to 5cm from the ground, so as to graft the root stock. The method provided by the invention is particularly suitable for the grafting and seedling cultivation of oil camellia and has the advantages that the grafting can be realized for multiple times within one year, so that the seedling cultivation cost is effectively reduced; through selecting and grafting appropriate root stock materials, the grafting survival rate is effectively increased; and through appropriately controlling conditions of pre-germination, seedling cultivation, and the like, the seedling cultivation period is effectively shortened.

The invention relates to a bud stock grafting method of Australian nuts. The grafting method comprises the following steps of: using Australian nuts for dibbled planting and culturing seedling sprouts of the nuts as bud stocks, using one-year-old branches which are lignified on the seed tree of Australian nuts as scion and grafting the branches on the bud stocks, temporarily planting the bud stocks in nutritional bags, planting the obtained nutritional bags with bud stocks on a seedling bed with shade net sheds and mulching film convex sheds and controlling the temperature and relative humidity in the sheds. The bud stock grafting method fully exerts the characteristics of strong regeneration capacity and vigorous growth of young tissues of the bud stocks, and effectively overcomes the defects that the forming layers of the young seedling stocks of the nuts are thin and the growth and coalescence are harder with the scions and grafting survival rate is low, thus greatly improving the grafting survival rate of the Australian nuts by more than 20 percent, shortening the period of out of nursery by more than one year, reducing the culturing cost of seedlings and increasing the economic benefit.

The invention discloses a method for grafting watermelons and cultivating grafted seedlings. The method comprises the following steps of: soaking seeds and accelerating germination: soaking stock seeds and scion seeds with clear water and carrying out moisturizing and germination acceleration for a certain time by a sterilizer; sowing and growing seedlings: horizontally placing and carrying out dibble seeding on the stock seeds subjected to germination acceleration in a nutrition cup, carrying out dibble seeding the scion seeds into a seedling-raising disk and cultivating the scion seeds in a greenhouse, wherein the scion seeds are seeded later than the stock seeds for 7-10 days; grafting: when a first rough leaf of the stock seeds is fully extended and two leaves of the scion seeds do not horizontally extend, grafting scions by adopting a top piece inserting method; and cultivating the grafted seedlings, strictly maintaining certain cultivation conditions and controlling insect pests at regular period. According to the method disclosed by the invention, the grafting survival rate is improved to be over 90 percent. In addition, nutrient soil is prepared from mud in a fish pond as a basic raw material, so that growing quality and grafting survival rate of the stock seedlings, the scion seedlings and the grafted seedlings are effectively improved.

The invention aims to provide a grafting method with minimum cut area for improving grafting survival rate, in particular a method for grafting different plants on the same plant at high survival rate and with simpleness, feasibility and wide application range. The grafting method comprises the following steps: (1) cion and parental stock selection, during which, an individual plant without disease and pest is selected as a parental stock and a wattle with the diameter similar to that of the parental stock is selected as a scion; (2) cion cutting, during which, 3 to 6 minutes of the scion is cut as a cion with one bud and one leaf (leafstalk only) left and the section perpendicular to the longitudinal direction of the wattle taken as a grafting surface; (3) parental stock cutting, during which, the parental stock is cut at the grafting height with the section perpendicular to the height direction of the parental stock taken as a grafting surface; (4) cion and parental stock combination, during which, the cion section and the parental stock section are tightly attached to align a cambium; and (5) interface binding and fixing, during which, an interface is fixed preliminarily by an adhesive tape and then the cion is tightly wrapped by a plastic film strip or a small-opening plastic bag so as to be fixed on the parental stock.

The invention discloses a mini watermelon grafting and seedlings raising method. The mini watermelon grafting and seedlings raising method comprises the following steps of stock seed selection, stock scion and seeding period, stock scion and grafting combined body, grafting combined body cutting and grafting combined body seedling. The grafting survival rate and the planting percent are improved. The grafting survival rate is more than 98% and the planting percent is more than 95% by means of the method, and the rafting survival rate is about 90% and the planting percent is 75-80% by means of a traditional grafting method. Compared with the traditional grafting method, the grafting survival rate is improved by more than 8%, and the planting percent is improved by 15-20%. A seedling raising period is shortened, namely the seedling raising period in winter and spring is 25-30 days by means of the mini watermelon grafting and seedlings raising method, but the seedling raising period is shortened, namely the seedling raising period in winter and spring is 40-50 days by means of the traditional grafting method, so that the seedling raising period in winter and spring is shortened by 10-20 days by means of the method compared with the traditional grafting method.

The invention provides a method for grafting carya illinoensis, the grafting temperature and humidity as well as stock and scion seedling cultivation before the grafting are strictly controlled, and the method is realized through the following steps of: building a potting cutting orchard, cultivating the stock and automatic temperature control and humidity control grafting; and because the control of the key carya illinoensis grafting links is reinforced, temperature difference between day and night is eliminated, the scion and the stock cut are prevented from expansion with heat and contraction with cold due to the temperature difference, enough water is ensured, the grafting failure due to the water loss of a scion bud is avoided, the grafting survival rate of the carya illinoensis can reach above 85%, and a better horticulture foundation is laid for successfully marketing the carya illinoensis.

The invention discloses a mango grafting cap cut-grafting method which can improve the grafting survival rate, and belongs to the technical field of fruit tree grafting. The mango grafting cap cut-grafting method comprises the steps of slantly downwards cutting from the lower portion of a bud (3) to form a scion slant cutting face (11), then upwards cutting along a forming layer of a scion (1) from a scion tip part (12) to form a scion long cutting face (13) and a cut scion phloem (14), slantly upwards cutting from one side of a root stock (2) to form a root stock slant cutting face (21), then downwards cutting along a forming layer of the root stock (2) from a root stock tip part (22) to form a root stock long cutting face (23) and a cut root stock phloem (24), pasting the scion long cutting face (13) and the root stock long cutting face (23) together in an aligning mode, wherein the cut phloems are pasted to slant cutting faces respectively, and finally conducting tight sealing and binding to finish the grafting process. The mango grafting cap cut-grafting method is simple and practical, high in survival rate and capable of used in popularization and application of mango seedling nursery stock grafting, top grafting seed exchanging, stem cutting seed exchanging and other aspects.

The invention discloses a cherry grafting method, and belongs to the technical field of agricultural planting. The method comprises the steps of stock preprocessing, scion preprocessing, grafting, later grated seedling managing and the like. Before cherry grafting, the stock is fertilized so that the nutrient supplying of the stock can be ensured; the scion is placed in the plant hormone to be immersed so that the wound healing after grafting can be promoted; during grafting, the incision attached position of the scion and the stock is coated with organic fertilizer and the like, and then enough nutrient can be supplied to the grafted scion, the incision of the scion and the stock can be fast healed as soon as possible, and therefore the grafting survival rate is improved and reaches to more than 97 percent.

The invention discloses a technique for grafting wild Chinese walnut, which comprises: cutting the fracture of a wild walnut or wingnut stock to form an inclined face (1); cutting off 3 to 5 centimeters of skin (2) of the upper end of the higher side of the inclined face (1) to form a 'V' shape (9); cutting off skin (5) in the direction of a bud on a cion branch; peeling the pulp skin layer (3) of the 'V' shape (9) of the stock to separate the pulp skin layer (3) from xylem (4); inserting the inclined plane (6) of a cion from top down while keeping the cion tightly adhering to the xylem (4) of the stock inward; sleeving the whole saw bite on the stock from the joint of the stock and the cion by using an agricultural mulching film; exposing cion branch; and fastening tightly. When the grafting technique is adopted, the operation is simple, the cost is low, and by using wild resources, the grafting survival rate is increased and fruiting is early.

The invention discloses a bud-stock grafting method for a camellia oleifera abel green branch with leaves. According to the method, a half-lignified green branch which is selected as a scion is grafted onto a nutritional bag stock seedling. A method for raising the green branch seedling with leaves through bud-stock grafting comprises the following steps of: seeding in a sand bed, bedding out at the seedling stage, and transplanting a nutrient cultivation stock seedling, wherein a stock is a camellia oleifera abel seedling, so that the root system is developed, and the capacity of absorbing inorganic nutrients and water is strong; the lignification degree of the scion is low, and tissue cells are active, so that a callus is formed quickly; and the reserved leaves can perform photosynthesis to generate organic nutrients for healing a graft union, so that the grafting survival rate is high, and the survival rate of forestation on mountains is higher than that of bud-stock grafted seedlings. Therefore, by adopting a novel technology of clonal reproduction for grafting the half-lignified green branch with the leaves, the grafting survival rate is more than 90 percent, the height of an annual grafted seedling is higher than 40cm, the ground diameter of the annual grafted seedling is 0.4cm, the root system is developed, the survival rate of the afforestation on the mountains is high, and stock seedlings of all seedling ages can be grafted all the year round as long as the stock seedlings have half-lignified green branches. The bud-stock grafting method for the camellia oleifera abel green branch with the leaves is suitable for large-scale industrial seedling raising application and the like. By adopting the novel bud-stock grafting method, the technical problem of low survival rate of forestation planting by using the bud-stock grafted seedlings is solved.

The invention relates to a breeding method for grafting and cultivating other types and breeds of hibiscus economic and ornamental plants by taking an extremely salt-resistant hibiscus hamabo as a wildstock to improve the salt resistance of scion. The pot experiment proves that the growth vigor of hibiscus which is grafted and propagated by taking the hibiscus hamabo as the wildstock is exuberant,the grafting survival ratio is high (above 90 percent), the hibiscus is resistant to the soil salt above 8 thousandth, but the hibiscus propagating by cuttings is moderately damaged by 4 thousandth soil salt. The hibiscus plant after being improved can be popularized to be cultivated and applied on the wide coastal shoal, thereby improving the utilization and yield of the land resources on the coastal shoal; at the same time, the breeding method has the great significance on enriching the ecological landscape of the coastal shoal and improving the ecological environment of the coastal shoal.

The invention discloses a grafting method taking masson pine tissue culture seedlings as stocks. The grafting method comprises processes of stock cultivation, scion preparation, seedling grafting and post-grafting management and protection; by taking the masson pine tissue culture seedlings cultivated for 4 to 6 months as the stocks and collecting bud sticks germinating on stock plants as scions, grafting is performed by adopting a split grafting method in December to January or in March to April; grafted seedlings are cultivated under the conditions of suitable light and humidity, and water and fertilizer management and pest control are performed by a conventional method. According to the grafting method disclosed by the invention, the masson pine tissue culture seedlings with developed roots, vigorous metabolism and rapid growth are cultivated through a tissue culture technological means to serve as the stocks, therefore not only can the seedling period of the stocks be shortened and the grafting survival percentage be increased but also the genetic gain of an excellent variety is ensured; adverse effects brought by the fact that heterogeneous-stock grafting is performed by taking slash pines as the stocks widely in masson pine grafting in production at present are effectively avoided, and the working effect of masson pine tissue culture stocks is greatly promoted; the grafting method has a broad application prospect, and has better economic benefit, social benefit and ecological benefit.

The hetero stock grafting process for pecan includes adopting thin-shell pecan stock and common pecan scion, raising the soil temperature for the stock to over 10 deg.c and cleft grafting. The present invention has the positive effects of raised grafting survival rate and capacity of providing high quality pecan tree.