721 results about "Graft survival" patented technology

Methods are provided for evaluating a subject for graft survival, e.g., in terms of predicting graft survival, identifying the presence of a deleterious graft condition, such as CAN and DT, identifying the severity and class of acute rejection, etc, in a subject are provided. In practicing the subject methods, the expression of at least one gene in a sample from the subject, e.g., a blood or biopsy sample, is assayed, e.g., at the nucleic acid and/or protein level, to evaluate the subject. Also provided are compositions, systems and kits that find use in practicing the subject methods. The methods and compositions find use in a variety of applications.

The invention relates to a seedling growing method of apocarya and belongs to the technical field of fruit trees and forest cultivation. The method includes the steps of sprouting breeding, grafting and post-grafting management. By means of the method, a large amount of scions are obtained each year and are grafted on the stock and the seedling with strong affinity in due time, so that stock grafting survival rate of the apocarya is improved. Grafted seedling preserving rate after large-amount grafting is over 90%. Further, the method achieves tree body stuntedness, shortens the juvenile phase and enables tree bodies to fruit earlier in the fourth year after grafting. The fruiting time is 6-8 years earlier than before. Simultaneously, the method provides a growing method for apocarya stock grafting seedlings and with strong operability for batch and variety seedling growing of the apocarya and greatly increases economical value of the apocarya.

The invention discloses a grafting method of camellia bud stocks, comprising the steps of: taking camellia bud seedlings which are not lignified or half-lignified as parental stocks, wherein, the camellia bud seedlings have 3-5cm height and more than 0.2cm diameter; taking the annual shoot camellia mother trees which are lignified or half-lignified as cions; grafting the stocks and the cions; packing grafting openings with preservative films when grafting; and sticking the preservative films with fire head irons. The invention adopts a preservative film-ironing technology, does not hurt the stocks, can completely seal the grafting openings when packaging, has good moisture retentiveness, has the hardly oxidized grafting openings which are fast healed, and has grafting survival rate which is high to 94.5%. Furthermore, the invention has simple and fast operation, can complete the grafting process by sticking the preservative films by means of light ironing with pastille fire head after being packaged, simplifies the complex grafting process of the camellia bud stocks, and is easy to learn, understand and operate. Therefore, farmers can fast skillfully master applied practical technique.

The invention relates to a cion treating and preserving method. Annual shoots or secondary shoots of over 0.3cm thickness that grow well and are free from damage by disease and insect are selected and cut off 0.5cm above sprout; cut-off shoots are sterilized and socked in 50ppm of solution of growth regulating agent for green plants for 2h, taken out and dried by airing, then mixed with commercial wax of 54 degrees, refined lard and colophony, ratio of which is 1:0.5:0.2, and heated to 100-200 DEG C; then the cion acquired is dipped into wax, wrapped by white thin film and put into a refrigerator for 1-6 months at the temperature of 0 DEG C to 4 DEG C; the cion is taken out, heated and transplanted as by a normal method in due time for grafting. Cion preservation: wax-sealed cion is stored at the temperature of 0 DEG C to 4 DEG C for further use; grafting is carried out when tree sap flows. The invention can prolong the preservation time of the cion and improve grafting survival rate; branches qualified for grating are treated in combination with winter pruning, and are taken out and grafted in due time.

The invention relates to 'a bark grafting method of apocarya' and belongs to the field of fruit trees and forest tree breeding. The method includes cutting a stock or a lignification branch of the apocarya in vigorous growth to the xylem, conducing inclining cutting on the portion above a cut to form a 45-degree cut, cutting downwards on an edge bark layer of the cut through a stem grafting knife for 3cm with cutting length as 3cm and cutting width determined by scion thickness and conducting scion branch processing and grafting. By means of the method, summer grafting survival rate of scions dormant in winter is greatly improved, the problem of scion grafting survival of ligneous plants like the apocarya which is introduced from the southern hemisphere in summer is effectively removed, and simultaneously grafting period of the apocarya can be prolonged.

The invention discloses a method for propagating apocarya seedlings. The method comprises the steps of selecting an annual apocarya seedling branch, shearing the branch to be the length being 10 cm, and soaking the lower end of the sheared branch into an 800 mg/L NAA solution for 4 h; placing a matrix into a paper bag, inserting a cutting into the matrix, enabling the paper bag obtained by cuttage to tightly abut against a mesh disc with a hole, and placing the paper bag on a seedbed; in the cutting rooting process, keeping the soil humidity to be about 70%, and keeping the temperature of the lower end of the cutting to range from 25 DEG C to 30 DEG C; conducting grafting 40 days after cuttage, adopting the bark peg grafting method, transferring the kraft paper bag, obtained after grafting, with the cutting into a large pot, filling the periphery of the pot with the matrix, pulling out 4/5 of the kraft paper bag, observing the grafting survival rate 15 days after grafting, completely removing the paper bag, and then conducting high-density nutrient and water management. According to the method, the apocarya nursery stock can grow more than 80 cm in the current year, and the survival rate is more than 90%.

The invention relates to a bud stock grafting method of Australian nuts. The grafting method comprises the following steps of: using Australian nuts for dibbled planting and culturing seedling sprouts of the nuts as bud stocks, using one-year-old branches which are lignified on the seed tree of Australian nuts as scion and grafting the branches on the bud stocks, temporarily planting the bud stocks in nutritional bags, planting the obtained nutritional bags with bud stocks on a seedling bed with shade net sheds and mulching film convex sheds and controlling the temperature and relative humidity in the sheds. The bud stock grafting method fully exerts the characteristics of strong regeneration capacity and vigorous growth of young tissues of the bud stocks, and effectively overcomes the defects that the forming layers of the young seedling stocks of the nuts are thin and the growth and coalescence are harder with the scions and grafting survival rate is low, thus greatly improving the grafting survival rate of the Australian nuts by more than 20 percent, shortening the period of out of nursery by more than one year, reducing the culturing cost of seedlings and increasing the economic benefit.

The invention discloses a method for grafting watermelons and cultivating grafted seedlings. The method comprises the following steps of: soaking seeds and accelerating germination: soaking stock seeds and scion seeds with clear water and carrying out moisturizing and germination acceleration for a certain time by a sterilizer; sowing and growing seedlings: horizontally placing and carrying out dibble seeding on the stock seeds subjected to germination acceleration in a nutrition cup, carrying out dibble seeding the scion seeds into a seedling-raising disk and cultivating the scion seeds in a greenhouse, wherein the scion seeds are seeded later than the stock seeds for 7-10 days; grafting: when a first rough leaf of the stock seeds is fully extended and two leaves of the scion seeds do not horizontally extend, grafting scions by adopting a top piece inserting method; and cultivating the grafted seedlings, strictly maintaining certain cultivation conditions and controlling insect pests at regular period. According to the method disclosed by the invention, the grafting survival rate is improved to be over 90 percent. In addition, nutrient soil is prepared from mud in a fish pond as a basic raw material, so that growing quality and grafting survival rate of the stock seedlings, the scion seedlings and the grafted seedlings are effectively improved.

The invention aims to provide a grafting method with minimum cut area for improving grafting survival rate, in particular a method for grafting different plants on the same plant at high survival rate and with simpleness, feasibility and wide application range. The grafting method comprises the following steps: (1) cion and parental stock selection, during which, an individual plant without disease and pest is selected as a parental stock and a wattle with the diameter similar to that of the parental stock is selected as a scion; (2) cion cutting, during which, 3 to 6 minutes of the scion is cut as a cion with one bud and one leaf (leafstalk only) left and the section perpendicular to the longitudinal direction of the wattle taken as a grafting surface; (3) parental stock cutting, during which, the parental stock is cut at the grafting height with the section perpendicular to the height direction of the parental stock taken as a grafting surface; (4) cion and parental stock combination, during which, the cion section and the parental stock section are tightly attached to align a cambium; and (5) interface binding and fixing, during which, an interface is fixed preliminarily by an adhesive tape and then the cion is tightly wrapped by a plastic film strip or a small-opening plastic bag so as to be fixed on the parental stock.

The invention discloses a mini watermelon grafting and seedlings raising method. The mini watermelon grafting and seedlings raising method comprises the following steps of stock seed selection, stock scion and seeding period, stock scion and grafting combined body, grafting combined body cutting and grafting combined body seedling. The grafting survival rate and the planting percent are improved. The grafting survival rate is more than 98% and the planting percent is more than 95% by means of the method, and the rafting survival rate is about 90% and the planting percent is 75-80% by means of a traditional grafting method. Compared with the traditional grafting method, the grafting survival rate is improved by more than 8%, and the planting percent is improved by 15-20%. A seedling raising period is shortened, namely the seedling raising period in winter and spring is 25-30 days by means of the mini watermelon grafting and seedlings raising method, but the seedling raising period is shortened, namely the seedling raising period in winter and spring is 40-50 days by means of the traditional grafting method, so that the seedling raising period in winter and spring is shortened by 10-20 days by means of the method compared with the traditional grafting method.

The invention discloses a cherry grafting method, and belongs to the technical field of agricultural planting. The method comprises the steps of stock preprocessing, scion preprocessing, grafting, later grated seedling managing and the like. Before cherry grafting, the stock is fertilized so that the nutrient supplying of the stock can be ensured; the scion is placed in the plant hormone to be immersed so that the wound healing after grafting can be promoted; during grafting, the incision attached position of the scion and the stock is coated with organic fertilizer and the like, and then enough nutrient can be supplied to the grafted scion, the incision of the scion and the stock can be fast healed as soon as possible, and therefore the grafting survival rate is improved and reaches to more than 97 percent.

The invention discloses a technique for grafting wild Chinese walnut, which comprises: cutting the fracture of a wild walnut or wingnut stock to form an inclined face (1); cutting off 3 to 5 centimeters of skin (2) of the upper end of the higher side of the inclined face (1) to form a 'V' shape (9); cutting off skin (5) in the direction of a bud on a cion branch; peeling the pulp skin layer (3) of the 'V' shape (9) of the stock to separate the pulp skin layer (3) from xylem (4); inserting the inclined plane (6) of a cion from top down while keeping the cion tightly adhering to the xylem (4) of the stock inward; sleeving the whole saw bite on the stock from the joint of the stock and the cion by using an agricultural mulching film; exposing cion branch; and fastening tightly. When the grafting technique is adopted, the operation is simple, the cost is low, and by using wild resources, the grafting survival rate is increased and fruiting is early.

The invention discloses a bud-stock grafting method for a camellia oleifera abel green branch with leaves. According to the method, a half-lignified green branch which is selected as a scion is grafted onto a nutritional bag stock seedling. A method for raising the green branch seedling with leaves through bud-stock grafting comprises the following steps of: seeding in a sand bed, bedding out at the seedling stage, and transplanting a nutrient cultivation stock seedling, wherein a stock is a camellia oleifera abel seedling, so that the root system is developed, and the capacity of absorbing inorganic nutrients and water is strong; the lignification degree of the scion is low, and tissue cells are active, so that a callus is formed quickly; and the reserved leaves can perform photosynthesis to generate organic nutrients for healing a graft union, so that the grafting survival rate is high, and the survival rate of forestation on mountains is higher than that of bud-stock grafted seedlings. Therefore, by adopting a novel technology of clonal reproduction for grafting the half-lignified green branch with the leaves, the grafting survival rate is more than 90 percent, the height of an annual grafted seedling is higher than 40cm, the ground diameter of the annual grafted seedling is 0.4cm, the root system is developed, the survival rate of the afforestation on the mountains is high, and stock seedlings of all seedling ages can be grafted all the year round as long as the stock seedlings have half-lignified green branches. The bud-stock grafting method for the camellia oleifera abel green branch with the leaves is suitable for large-scale industrial seedling raising application and the like. By adopting the novel bud-stock grafting method, the technical problem of low survival rate of forestation planting by using the bud-stock grafted seedlings is solved.

The invention discloses a grafting method taking masson pine tissue culture seedlings as stocks. The grafting method comprises processes of stock cultivation, scion preparation, seedling grafting and post-grafting management and protection; by taking the masson pine tissue culture seedlings cultivated for 4 to 6 months as the stocks and collecting bud sticks germinating on stock plants as scions, grafting is performed by adopting a split grafting method in December to January or in March to April; grafted seedlings are cultivated under the conditions of suitable light and humidity, and water and fertilizer management and pest control are performed by a conventional method. According to the grafting method disclosed by the invention, the masson pine tissue culture seedlings with developed roots, vigorous metabolism and rapid growth are cultivated through a tissue culture technological means to serve as the stocks, therefore not only can the seedling period of the stocks be shortened and the grafting survival percentage be increased but also the genetic gain of an excellent variety is ensured; adverse effects brought by the fact that heterogeneous-stock grafting is performed by taking slash pines as the stocks widely in masson pine grafting in production at present are effectively avoided, and the working effect of masson pine tissue culture stocks is greatly promoted; the grafting method has a broad application prospect, and has better economic benefit, social benefit and ecological benefit.

The hetero stock grafting process for pecan includes adopting thin-shell pecan stock and common pecan scion, raising the soil temperature for the stock to over 10 deg.c and cleft grafting. The present invention has the positive effects of raised grafting survival rate and capacity of providing high quality pecan tree.

The invention provides a grafting method of a superior walnut, which comprises the specific steps as follows: (1) cultivating a stock: selecting an ordinary walnut variety, wherein the sowing time is in October to December, the density is controlled at 5500-6000 plants per mu, and the basal diameter is 0.8-1.5cm; (2) grafting seedlings: a, collecting braches of the superior walnut and spring tips with the thickness of 0.8-1.5cm and cutting into the branch segment with the length of 12-15cm and 2-4 full buds left for scion; b, the grafting time lasts for 5-7 months, and the grafting air temperature is at 28-30 DEG C; and c, the grafting method adopts a budding method, and the grafting part is at 10-15cm from the stock to the ground and is wrapped by a plastic film; (3) later-period management: cutting the stock and protecting the bud after grafting for 10-20 days, and controlling the pests and disease and protecting the buds after 30 days; and (4) selling the seedlings: the seedlings are removed and sold step by step in the late October. By adopting the grafting method, the grafting survival rate of the walnut seedlings is improved to 85-90%, and the grafted walnut seedling begins to bear fruits after three years and achieves high yield after five years after being transplanted.

The invention belongs to the field of planting machinery and provides a grafting machine, which consists of a work table, a cutting engagement device and a seedling positioning device. The invention also provides a grafting method adopting the grafting machine. The seedling grafting machine has the beneficial effects that 1, the grafting mechanization is realized, and the problems of high work intensity and low work efficiency of artificial grafting are solved; 2, when the grafting machine provided by the invention is adopted, the cutting surfaces of stocks and cions are precisely aligned, at least one side of the forming layer is aligned, and the grafting survival rate is high; and 3, a wax coating mode is adopted for fixing the cutting surface combining part of the stocks and the cions, the wound infection is avoided, in addition, the seedling thickness increase is not influenced, and the binding rope unlocking treatment after the traditional grafting is omitted.